The invention provides a hyper-branched
DNA rolling circle amplification-based
virus detection sensor and a test method, the hyper-branched
DNA rolling circle amplification-based
virus detection sensor comprises a carbon-based
field effect transistor biosensor, the carbon-based
field effect transistor biosensor is a P-type device and sequentially comprises a substrate S I layer, an S < 2 > layer, a CNT channel layer and an HfO2
dielectric layer from bottom to top, and a source
electrode and a drain
electrode are symmetrically arranged on the S < 2 > layer; wherein the surface of the CNT channel layer is modified and fixed with a primer, and the primer is used for carrying out HRCA reaction on the interface of the channel layer to cause the current rise of the device; the
biosensor based on the carbon-based
field effect transistor is used for detecting viruses, the device reaches the micron level,
batch production is easy, cost is low, carrying is convenient, the ultrasensitivity of the carbon-based
field effect transistor to
surface charges is utilized, a large number of negatively charged
DNA molecules can be generated through the HRCA reaction, the detection process is short in time consumption, and the detection efficiency is high. And the defect of long detection time caused by low concentration of DNA of a
virus sample in the existing detection technology is overcome.