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446results about How to "Detection time is short" patented technology

Micro-fluidic device and method for detecting circulating tumor cell on basis of dimension

The invention provides a micro-fluidic device and a method for detecting circulating tumor cells on the basis of dimensions. The micro-fluidic device comprises a solution storage chamber, a micro-fluidic chip, a waste liquid collecting syringe and a power system which are connected in sequence, wherein the micro-fluidic chip is made of a glass substrate layer and a PDMS (Polydimethylsiloxane) chip layer in a laminated manner; the PDMS chip layer comprises an injection port, a block mass filtering area, a target sell screening area and a discharge port which are communicated in sequence; the block mass filtering area is formed by a column array; the target cell screening area comprises a plurality of main pipelines and side pipelines which are extended in a parallel manner at intervals and are communicated through filtering channels; the front ends of the main pipelines are opened; the filtering channels are formed in the rear ends of the main pipelines; the front ends of the side pipelines are sealed; the rear ends of the side pipelines are opened; the main pipelines and the side pipelines have a first height greater than the dimensions of the circulating tumor cells; the filtering channels have a second height smaller than the dimensions of the circulating tumor cells. The micro-fluidic device and the method for detecting the circulating tumor cells on the basis of the dimensions are good in sensitivity, simple to operate, low in cost, large in flux and short in time.
Owner:SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI

Method and device for rapidly and quantitatively detecting tumor marker with immunochromatography test strip marked by quantum dots

The invention relates to a method and a device for rapidly and quantitatively detecting a tumor marker with an immunochromatography test strip marked by quantum dots. The method comprises the following steps of: replacing collaurum particles by using quantum dots as a signal marker, coupling with an antibody corresponding to the tumor marker to be tested, and spraying or directly coating on a conjugate releasing pad, wherein the antibody on another site corresponding to the tumor marker and a second antibody are coated on a nitrocellulose membrane to form a T line and a C line respectively; assembling a sample pad, a combining pad, a reaction membrane and a water absorbing pad according to a certain sequence to prepare the immunochromatography test strip; detecting qualitatively and quantitatively according to fluorescent stripes presented on the T line and the C line and the strength of fluorescence; and providing a quantum dot fluorescence immune detection device. The detection device is simple, the operation is simple and rapid, the time consumption for detection is short, and the result judgment is easy. The method and device are particularly suitable for early-stage screening, diagnosis, judgment prediction and prognosis of the tumor markers in households, communities, hospitals and the like, and evaluation of treatment effect and followed observation of high risk groups.
Owner:TIANJIN UNIV

Establishment method for small fragmental DNA (Deoxyribose Nucleic Acid) library based on Ion ProtonTM sequencing platform and application of establishment method

The invention belongs to the field of gene sequencing, and discloses an establishment method for a small fragmental DNA (Deoxyribose Nucleic Acid) library based on an Ion ProtonTM sequencing platform, and a relevant solvent composition solution. The establishment method disclosed by the invention comprises the steps of DNA sample extraction, tail end repairing, fragment selection, joint connection, PCR (Polymerase Chain Reaction) amplification, library detection, high-throughput sequencing and the like, wherein a reagent I, a reagent II and a reagent III are respectively adopted in the steps of tail end repairing, joint connection and PCR amplification. The establishment method disclosed by the invention is precise and simple in step, simple to operate, small in reagent variety and low in cost, the loss and waste of the library are reduced, and the working efficiency is improved; in addition, the establishment method can be applied to noninvasive antenatal diagnosis on pregnant woman peripheral blood fetus chromosome aneuploid based on the Ion ProtonTM sequencing platform, the diagnosis result is high in accuracy rate and is safe and economic, and the birth rate of chromosome aneuploid fetuses can be effectively controlled.
Owner:BOAO BIOLOGICAL CO LTD

New type nucleic acid detection method and detector tube

The invention discloses a new type nucleic acid detection method. Lysate, cleanout fluid and reaction liquid in a detector tube are isolated by arranging a plurality of separating layers in the detector tube, meanwhile, through the control on an external heat source, the separating layers are respectively melted in different detection stages so as to control time sequence in all detection stages, a mixing device which is controlled by an external magnetic body to move and can be magnetized is used in the lysate, mixes samples added into the detector tube, and nucleic acid extraction and purification of the samples are realized, then nano magnetic beads in the detector tube are driven by the external magnetic body to pass the cleanout fluid and then enter into the reaction liquid, nucleic acid carried by the nano magnetic beads is purified in the cleanout fluid and then is eluted in the reaction liquid, an amplified reaction of the nucleic acid in the reaction liquid occurs, finally, an external device realizes the detection on the sample nucleic acid by the optical detection, so that a plurality of steps of performing extraction, purification, cleaning, elution and amplified reaction on the nucleic acid in one detector tube are realized.
Owner:USTAR BIOTECHNOLOGIES (HANGZHOU) CO LTD

Three-dimensional visual inspection method, system and device based on shape attention mechanism

PendingCN110879994AAddressing the lack of shape priorsEasy to detectThree-dimensional object recognitionPattern recognitionVoxel
The invention belongs to the field of computer deep reinforcement learning and pattern recognition, particularly relates to a three-dimensional visual inspection method, system and device based on a shape attention mechanism, and aims to solve the problems that the precision of a single-stage detector is lower than that of a two-stage detector, and the two-stage detector consumes a large amount oftime and is not suitable for a real-time system. The method comprises the steps of representing point cloud data through three-dimensional grid voxels; extracting features and encoding a spatial sparse feature map; after projecting to a top view, extracting different scale features; combining the features by adopting a deconvolution layer; extracting a shape attention feature map through the attention weight and the convolutional coding layer; and obtaining a target category, a target position, a target size and a target direction through the target classification network and the regression positioning network. According to the method, a sampling strategy based on distance constraint and an attention mechanism based on shape prior are used, instability caused by uneven data distribution is relieved, the problem that a single-stage detector lacks shape prior is solved, and the method is high in precision, short in consumed time, high in real-time performance and good in robustness.
Owner:INST OF AUTOMATION CHINESE ACAD OF SCI

Kit, primers, probe sequence and method for detecting fetus chromosome aneuploid

The invention provides a kit, primers, probe sequences and a method for detecting fetus chromosome aneuploid. The kit comprises the multiple pairs of specific primers and a plurality of specific Taqman probes all of which are designed according to 13#, 18# and 21# chromogene sequences of the human, and further comprises a reaction system used for digital PCR, wherein the nucleotide sequence of the specific primers and the specific Taqman probes is SEQ ID NO: 1-90. The detection method comprises the steps that firstly, the specific primers and the specific Taqman probes are designed according to the 13#, 18# and 21# chromogene sequences of the human; secondly, peripheral blood of a (12-23)-week pregnant woman is collected for plasma DNA extracting; thirdly, the reaction system of the digital PCR amplification gene sequence is prepared; fourthly, the numbers of 13#, 18# and 21# chromosomes of a fetus are calculated through digital PCR detection for the number of the chromosomes, digital PCR is used for detecting FAM and VIC fluorescence of all reaction units of a chip, and the copy number of the 13# chromosome, the copy number of the 18# chromosome and the copy number of the 21# chromosome are calculated respectively. By means of the kit and the method, the number of the 13# chromosome, the number of the 18# chromosome and the number of the 21# chromosome of the fetus can be efficiently, quickly and accurately detected without invasion, and the kit and the method are beneficial to clinic application and popularization.
Owner:内蒙古因诺生物技术有限公司

Lane line detection method based on vanishing point estimation and semantic segmentation

The invention relates to the technical field of image processing, and provides a lane line detection method based on vanishing point estimation and semantic segmentation. The lane line detection method comprises the following steps: firstly, detecting a vanishing point through a convolutional neural network, secondly, performing inverse perspective transformation of self-parameter learning throughestimated vanishing point coordinates, and projecting an image into an overlooking view angle easy for network learning. In the top view, binary segmentation is carried out through a semantic segmentation network, then post-processing instantiation is carried out, and a lane line fitting equation is obtained and displayed in an original image. According to the method, the problem of lane line detection in different road scenes is solved by utilizing the strong feature extraction capability of the convolutional neural network, and the time of instantiation operation of a lane line detection algorithm is saved. According to the technical scheme, the edge of the fuzzy lane line can be effectively detected, noise is suppressed, time consumed for detection of vanishing points and straight lines is short, accuracy is high, and lane line recognition real-time performance is improved.
Owner:SOUTH CHINA UNIV OF TECH

Method and system for automatically detecting ultrasound image of fetal head circumference

The invention provides a method and a system for automatically detecting an ultrasound image of a fetal head circumference. The method comprises the following steps: acquiring the ultrasound image of the fetal head circumference, and acquiring a corresponding binary image; acquiring all curve arc sections of the binary image, and forming a curve arc section set by the acquired curve arc sections; using each curve arc section in the curve arc section set as a base arc section in sequence; classifying other curve arc sections, which are different curve arc sections on the same ellipse with respect to the current base arc section, into one group, to form curve arc section groups; acquiring the curve arc section group with a maximum ellipse matching rate; judging whether the maximum ellipse matching rate is greater than a preset ellipse matching rate threshold of the system or not, if so, determining an ellipse matched with the current curve arc section group as a fetal head circumference ellipse, and acquiring a perimeter of the fetal head circumference according to the fetal head circumference ellipse; otherwise, judging that a matching result is wrong, and acquiring the ultrasound image of the fetal head circumference again. The method and the system for automatically detecting the ultrasound image of the fetal head circumference are simple in calculation, accurate in detection and high in robustness.
Owner:VINNO TECH (SUZHOU) CO LTD

Method for detecting content of mud in limestone

The invention relates to the technical field of detection of mixing materials, in particular to a method for detecting content of mud in limestone. The detection method particularly comprises the steps as follows: A, preparing a standard sample, that is, obtaining mine soil samples to prepare mud powder with a grain size smaller than 0.08mm, obtaining mine limestone, screening limestone samples with a grain size larger than 0.08mm and limestone powder with a grain size smaller than 0.08mm, and preparing a methylene blue testing sample by using the mud powder, the limestone powder and the limestone according to the following principles; B, detecting consumption V of methylene blue solution of the standard sample obtained in the step A by using a methylene blue testing method and building a corresponding relationship among V, P and W; and C, obtaining W1 of the to-be-detected sample obtained through a passing rate P1 of the to-be-detected sample with a grain size smaller than 0.08mm, the consumption V1of a methylene blue solution and the relationship among the V, the P and the W in the contrast step B, so as to obtain the content of mud in the sample to be detected. The detection method is accurate in test result and short in time for detection.
Owner:SICHUAN ESHENG CEMENT GROUP

Kit for detecting equine piroplasmosis and preparation method and using method thereof

The invention discloses a detection kit for identifying and diagnosing whether an equine animal is infected with piroplasmosis or not, and a preparation method and a using method thereof. A detection film for detecting equine piroplasmosis and a piroplasmosis universal primer are arranged in the kit for detecting the equine piroplasmosis. The using method of the kit comprises the following steps:extracting DNA (deoxyribonucleic acid) from blood of a horse to be detected, further using the piroplasmosis universal primer to perform amplification, combining an amplification product with the detection film, enabling biotin at the terminal of a PCR (polymerase chain reaction) product to be in action with streptavidin-peroxidase, and then playing a catalysis role in enabling a substrate of peroxidase, namely an enhanced chemiluminescence reagent (ECL-detection) to emit fluorescence, thereby exposing an X-photosensitive film; and performing development and fixing treatment on the film so asto emerge a shadow in a region corresponding to specificity hybridization of a probe on the detection film and the PCR product, and further judging whether the detected animal is infected with the piroplasmosis or not according to whether the shade is left on the film or not.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI

FCM (flow cytometry) based method for quickly and quantitatively detecting total viruses in freshwater environment

An FCM based method for quickly and quantitatively detecting total viruses in a freshwater environment belongs to the field of biotechnology and water environment monitoring, and comprises six main steps including film filtration, settlement, storage, coloration, dilution and detection. The specific operation processes are as follows: passing a sampled water sample through a 0.22 micrometer filter film; adding glutaraldehyde, settling glutaraldehyde for 15 minutes at the temperature of 4 DEG C, and then refrigerating glutaraldehyde by liquid nitrogen; storing glutaraldehyde in a refrigerator at the temperature of minus 80 DEG C; adding Na2EDTA and fluorescent dye SYBRGreen I before detection, and performing coloration for 10 minutes at the temperature of 80 DEG C; diluting the sample by Milli-Q water after the sample cools down to the room temperature; and using the flow cytometer to detect. The method disclosed by the invention overcomes the problem that the present detection method for freshwater total viruses is time consuming and labor consuming, realizes quick and quantitative detection of the freshwater viruses, and has a wide application prospect in fields such as freshwater virus detection and aquatic bioecology study.
Owner:NANKAI UNIV
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