The invention relates to the technical field of
biology, and discloses a kit for detecting
chlamydia trachomatis and an application of the kit. The kit comprises a capture probe, a sulfydryl
signal probe, a carrier, a silver
staining initiating liquid and silver enhancement liquid, the capture probe is modified by a first modifier, the sulfydryl
signal probe is marked by gold nano-particles, and asecond modifier wraps the carrier. According to the kit, specific conserved sequences in the
chlamydia trachomatis are selected as templates to design the sulfydryl
signal probe marked by the gold nano-particles and the capture probe modified by the first modifier, the sulfydryl signal probe, the capture probe and target sequence specificity are combined to form a
sandwich compound, the sandwichcompound can be anchored on the carrier through the second modifier capable of being combined with the first modifier, silver shells are formed on the surfaces of the gold nano-particles under matching of the silver
staining initiating liquid and the silver enhancement liquid through catalytic actions of the gold nano-particles, different developing signal differences are achieved, and efficient,special, high-
throughput and sensitive detection of the
chlamydia trachomatis can be achieved by naked eyes and micro-plate readers.