The invention belongs to the field of cytobiology and particularly relates to a culture medium and method for culturing DCs. The culture medium for culturing the DCs comprises a first component, a second component and a third component. The first component is an RPMI1640 culture medium containing bovine serum albumin, interleukin 4, GM-CSF and stem cell factors (SCF). The second component is an RPMI1640 culture medium containing bovine serum albumin, interleukin 14, interleukin 2, interleukin 4 and GM-CSF. The third component is an RPMI1640 culture medium containing bovine serum albumin, CD40L, GM-CSF and interleukin 4. The result shows that the antigen presentation capacity of the DCs cultured by the culture medium is higher, the sensitization capacity to T cells is higher, and the killing capacity to tumors is greatly improved. Use of serum is avoided, and the risk of pathogenic microorganisms is relieved.