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Method for amplifying lymphocyte by interleukin 15 receptor and interleukin 2 complex

A technology of lymphocytes and interleukins, applied in the field of immunology, can solve problems such as inability to enhance innate immunity and stress immunity, and achieve the effect of improving immunity and resisting viruses and bacteria

Inactive Publication Date: 2010-06-16
浙江中赢控股集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the biological effects of interleukin 15 need to be realized through the formation of ligand-receptor complexes, it is currently not possible to fully utilize interleukin 15 to expand NK and CD8 cells in vitro or in vivo to enhance innate immunity and acquired adaptive immunity
In fact, direct treatment of NK and CD8 cells with interleukin-15 receptors can form interleukin-15 and interleukin-15 receptor heterodimers, but this heterodimer has an antagonistic effect on interleukin-15

Method used

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  • Method for amplifying lymphocyte by interleukin 15 receptor and interleukin 2 complex
  • Method for amplifying lymphocyte by interleukin 15 receptor and interleukin 2 complex
  • Method for amplifying lymphocyte by interleukin 15 receptor and interleukin 2 complex

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Interleukin 15 receptor (IgG1 Fc)-interleukin 2 complex and interleukin 15 receptor (IgG1 Fc)-interleukin 15 complex in vitro expansion of lymphocytes.

[0039] NK and CD8 cells from healthy donors were cultured in RPMI1640 supplemented with 10% human serum. Three days after interleukin 2 was added to the culture medium, the interleukin 15 receptor (IgG1 Fc)-interleukin 2 complex ( figure 1 ) or interleukin-15 receptor (IgG1 Fc)-interleukin-2 complexes were cultured for several days. Interleukin 2 and Interleukin 5 were used as controls, such as figure 2 and image 3 shown. The number of NK and CD8 cells increased significantly under the action of interleukin 2, interleukin 15 receptor (IgG1 Fc)-interleukin 15 complex and interleukin 15 receptor (IgG1 Fc)-interleukin 2 complex. However, the increase of cells in the control group was far less obvious than that in the experimental group under the single action of interleukin-2 and interleukin-15. An increase in cell...

Embodiment 2

[0042] Lysis of Tumor Cells by NK Cells Activated by Interleukin 15 Receptor (IgG1 Fc)-Interleukin 2 Complex and Interleukin 15 Receptor (IgG1 Fc)-Interleukin 15 Complex

[0043] Previous studies have shown that injecting IL-15 into mice with tumors can enhance the anti-tumor ability of mice and delay the lifespan of mice. The present invention uses interleukin 2, interleukin 15 receptor (IgG1 Fc)-interleukin 2 complexes and NK cells activated by interleukin 15 receptors (IgG1 Fc)-interleukin 15 complexes to co-culture with tumor cells to study the use of interleukin 15 Antitumor feasibility of receptor (IgG1 Fc)-interleukin 15 complex and interleukin 15 receptor (IgG1 Fc)-interleukin 2 complex.

[0044] NK cells from healthy donors were isolated from lymphocytes and sequentially added interleukin 2, interleukin 15 receptor (IgG1 Fc)-interleukin 2 complex or interleukin 15 receptor (IgG1 Fc)-interleukin 15 complex activation for 14 days . SK-N-AS, K562, and CD19+ chronic lym...

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Abstract

The invention discloses a technology in the aspect of immunology, in particular to a method for amplifying natural killer (NK) cells and CD8 lymphocyte by interleukin 2 and interleukin 15 receptor (IgG1 Fc) and interleukin 2 complex. A polypeptide complex is cultured by an interleukin 2 and the interleukin 15 receptor which are protein having the expression function, and the aim of changing the immune state is achieved by the lymphocyte or a lymphocyte precursor cell activated and amplified by the polypeptide complex. The patient is helped to resist a tumour, viruses and bacteria by improving the immunity through the interleukin 15 receptor (IgG1 Fc) and interleukin 2 complex, and the realizing effect is good. The invention has wide prospect in the aspect of immunology.

Description

technical field [0001] The invention belongs to the field of immunology, specifically refers to the use of interleukin 2, interleukin 15 receptor (IgG1 Fc)-interleukin 2 complex to expand natural killer cells (NK) and CD8 lymphocytes. technical background [0002] Previous studies have shown that interleukin 2 and interleukin 15 are involved in the activation process of innate immunity and acquired adaptive immunity of NK cells, dendritic cells and CD8 cells. However, previous efforts to expand these cells with interleukin-15 or interleukin-2 in vitro and in vivo have been limited. [0003] Interleukin 15 and interleukin 2 activate CD8 and NK cells by binding to specific receptors on the surface of CD8 and NK lymphocytes. Interleukin 2, interleukin 15 and interleukin 15 receptor are strongly expressed in activated dendritic cells and monocytes. Interleukin 2 and interleukin 15 can respectively bind to interleukin 15 receptors to form heterodimer complexes to activate CD8 a...

Claims

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Application Information

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IPC IPC(8): C12N5/0783
Inventor 徐以兵吴忠福董升炬
Owner 浙江中赢控股集团有限公司
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