36 results about "Immune mechanisms" patented technology

The invention discloses a polyphenol-based tissue adhesive for promoting wound healing and a preparation method thereof, and relates to the technical field of medical tissue adhesives. The preparation method of the tissue adhesive comprises the following steps: step 1, preparing a polyphenol compound and recombinant mussel mucin into a solution, and uniformly stirring for later use, namely a solution A; 2, preparing a temperature response type high polymer material into a solution for later use, namely a solution B; and 3, mixing the solution A prepared in the step 1 and the solution B prepared in the step 2, fully and uniformly mixing, shaking and centrifuging, and discarding the supernatant. By selecting the temperature response type high polymer material, the tissue adhesive is endowed with the characteristic of temperature sensitivity, so that the modulus of the adhesive at the human body temperature is improved, and the colloid contraction can further promote wound closure. The mussel mucin is introduced to stimulate collagen regeneration of the wound part, activate the immune mechanism and remodel the wound microenvironment so as to achieve the purpose of promoting healing.

The invention discloses an auxiliary diagnosis system for renal transplantation rejection and typing thereof based on a peripheral blood immune cell map. Comprising a peripheral blood single cell acquisition unit, a mass spectrum flow cytometry unit, an original data preprocessing unit, a data conversion and normalization processing unit, a peripheral blood immune cell grouping acquisition unit, a peripheral blood immune cell map construction unit, a feature vector acquisition unit, a rejection prediction unit and a rejection typing prediction unit. According to the invention, a kidney transplantation two-stage diagnosis architecture is created for the first time; rejection detection and typing identification are realized through classification of an independently trained random forest binary classifier, and different TCMR / ABMR immune mechanisms are accurately matched; the traditional biopsy limitation is broken through, an auxiliary diagnosis scheme with high precision, low damage, moderate cost and typing identification capability is provided for the kidney transplantation rejection, and the method has important significance on timely discovery of the rejection of a kidney transplantation patient and improvement of prognosis.

The invention discloses a multichannel tissue-derived neutrophil detection chip and an application system, the chip is a multichannel detection chip integrating migration-related markers and functional markers, rapid and synchronous identification of neutrophil tissue sources is realized, the migration-related markers are CD62L and CXCR4, the functional markers are CD44 and MPO, and the migration-related markers are CD62L and CXCR4. According to the method, migration related markers and functional state markers are subjected to integrated and collaborative analysis, tissue sources are locked from the cell state and function two dimensions, four-channel parallel detection is performed, and the tissue sources of the neutrophil are accurately judged through expression profile modes of the four components instead of a single marker, so that the neutrophil detection accuracy is improved. According to the method, the neutrophil tissue source is rapidly, synchronously and accurately identified, the technical bottleneck of neutrophil tissue source detection is broken through, high efficiency, accuracy and universality are achieved, and an efficient and accurate tool is provided for immune mechanism research and clinical diagnosis.

The invention discloses an underwater robot networking environment sensing method based on an immune mechanism, and the method comprises the steps: distributing a recognition main body according to the environment sensing task demands of an underwater robot, constructing an environment database, adding a dynamic updating mechanism, and updating the environment database, a sensor is arranged for an underwater robot to collect original data, the original data serve as antigens to be matched with recognition subjects, the recognition subjects are screened and copied according to the matching result, the complexity degree of the current underwater environment is obtained to determine the number of the copied recognition subjects, and the copied recognition subjects are evaluated and optimized. And executing an environment perception task according to the optimized copy identification main body and an initial identification main body of the copy identification main body, sharing information through networking, setting a task expected result, calculating a difference degree between an actual result and the task expected result, and performing adjustment according to difference degree output. Therefore, the dynamic adaptability and the cooperation efficiency of the underwater robot networking in the underwater environment sensing process are improved.

The invention discloses a toxoplasma gondii attenuated strain with deletion of a Tgsrs51 gene and application of the toxoplasma gondii attenuated strain, and belongs to the technical field of gene engineering. A Tgsrs51 gene is deleted in a genome of the toxoplasma gondii attenuated strain; and the ID (Identity) number of the Tgsrs51 gene on a ToxoDB (Database) website is TGME49308840. A Tgsrs51 gene deleted strain of a toxoplasma gondii type II Pru strain is constructed by utilizing a CRISPR-Cas9 technology, and experiments prove that the Tgsrs51 gene deletion can remarkably reduce the invasion efficiency of the toxoplasma gondii Pru strain, the secretion capacity of micronematosome MIC2 and the tachyzoite proliferation rate, lead to in-vitro growth limitation, remarkably improve the survival rate of infected mice and remarkably reduce the number of intracerebral cysts. The invention provides a theoretical basis for researching a molecular mechanism and an immune mechanism of toxoplasma gondii pathopoiesis, and provides a new strategy for prevention and control of toxoplasmosis.

The invention discloses a distributed energy agent regulation and control method and system based on deep reinforcement learning, and the method comprises the following steps: collecting distributed energy operation data and network constraint data in a micro-grid or park power distribution network, and carrying out the preprocessing; constructing a reinforcement learning environment state representation vector; configuring a distributed energy agent set; obtaining a disturbance antigen fingerprint entry set, and generating an immunoaffinity score sequence; generating a normal regulation and control action instruction set, combining and issuing to execute or enter a perturbation sampling process; generating a feasible action domain and a perturbation action candidate set, and executing exploration control contract verification; a target perturbation action is selected and issued for execution or a corrected perturbation action is generated and issued for execution; and updating the deep reinforcement learning strategy network. According to the method, deep reinforcement learning and disturbance immune mechanisms are adopted, distributed energy intelligent agent cooperative regulation and control are achieved, and the method has the advantages of being high in safety, high in adaptability and good in stability.

The invention provides the primer and the detection kit for detecting the culicoides chromogen immune mouse B cell receptor repertoire, the amplified B cells and plasma cell clone can be identified and quantified by screening the BCR repertoire by utilizing the kit, and the kit has the advantages of high throughput, rapid and accurate detection and the like. Experiments show that a basis is provided for the research on the immune mechanism of the culicoides leucoides allergen.

The invention belongs to the technical field of bioengineering, and particularly relates to a preparation method and application of an N protein polyclonal antibody of a horse coronavirus. The polyclonal antibody is prepared by truncation expression of the horse coronavirus N protein, and a material is provided for research on inhibition of host innate immune mechanism by the horse coronavirus N protein. Prokaryotic expression is carried out on the N protein of the equine coronavirus, the polyclonal antibody is prepared, and a method and a material are provided for molecular research and mechanism research of the equine coronavirus. According to the invention, in combination with bioinformatics analysis of an N protein of the horse coronavirus, a pGEX-6p-N3 recombinant plasmid is constructed, and prokaryotic expression is carried out, so that a purified recombinant protein is obtained. After a mouse is immunized by the recombinant protein, a polyclonal antibody is obtained, an indirect ELISA method detects that the polyclonal antibody has good titer, Western Blot detection has good reactivity, and the polyclonal antibody can be applied to an indirect immunofluorescence test.

The invention provides a method for detecting subtype pSTAT4 and Ki67 signals of peripheral blood T lymphocytes of machin through flow cytometry, and belongs to the technical field of biological detection. According to the method, the integrated analysis of the multi-dimensional functional state of the cynomolgus monkey T lymphocyte is realized, key signal channel activation and proliferation information of different subgroup cells can be synchronously obtained through a single experiment, and the detection comprehensiveness and efficiency are greatly improved. According to the method, an accurate tool capable of directly evaluating the response intensity and proliferation potential of T cells is provided for immune mechanism research based on a machin model, particularly immune therapy or disease research related to regulation and control of pathways such as IL-12 / STAT4 and the like, and the data dimension and reliability of preclinical immunological evaluation are remarkably enhanced.

The application belongs to the technical field of bio-agriculture, and particularly relates to a gene MOS4 and application of an encoded protein thereof in regulation of plant resistance to parasitic Phytophthora. MOS4 Through gene function research, it is found that overexpression of the gene MOS4 significantly improves the plant resistance to parasitic Phytophthora, and that the expression of the gene MOS4 weakens the plant resistance to parasitic Phytophthora, and that the gene MOS4 positively regulates the plant resistance to parasitic Phytophthora. Further immune mechanism research finds that the gene MOS4 regulates the related genes of a salicylic acid (SA) signal path, interacts with a transcription factor ERF019, and jointly activates the plant immune response. In addition, a dual luciferase experiment proves that the gene MOS4 and the gene ERF019 cooperatively regulate the transcription of PR1. The application provides a new molecular target for prevention and treatment of plant diseases caused by parasitic Phytophthora, and has important agricultural application value.

This invention belongs to the field of bio-agricultural technology, specifically relating to genes. DISC1 The application of its encoded proteins in regulating plant resistance to Phytophthora parasiticans. This invention, through the study of... DISC1 Studies on the function of genes and their encoded proteins have revealed that... DISC1 Negative regulation of plant resistance to Phytophthora parasiticum. Overexpressed genes. DISC1 Significantly reduces plant resistance to Phytophthora parasiticum, gene DISC1 The mutant plants exhibited significantly enhanced resistance to Phytophthora parasiticum. Further research into the immune mechanism revealed that the gene... DISC1 This invention regulates plant immune responses by modulating genes related to the jasmonic acid (JA) signaling pathway. It provides a novel gene resource for the control of plant resistance to Phytophthora parasiticum.

The present application relates to the field of genetic engineering, in particular to a sgRNA group targeting DYA gene, a primer pair for amplifying target sequence, a plasmid group and application thereof. The sgRNA group designed by the present application edits the sheep DYA gene, and after gene editing, large fragment deletion occurs and causes frame shift mutation, which causes great damage to the protein structure and function encoded by the DYA gene. The experimental results show that different fragment lengths of gene editing can be caused in the target region, the overall editing efficiency of the first exon reaches 76.4%, and the overall editing efficiency of the second exon reaches 84.09%. Editing occurs on both sets of chromosomes in the genome (i.e. homozygote): the homozygote editing efficiency of the first exon is 30.9%, and the homozygote editing efficiency of the second exon is 9.1%. The sgRNA group provided by the present application has important significance in the study of ruminant evolution and related immune mechanisms.

This invention discloses a method for constructing a PBMC-derived humanized mouse scleroderma model. Human peripheral blood mononuclear cells are transplanted into immunodeficient mice, and a human immune system is reconstructed in the mice. Bleomycin is injected into the local skin of the mice in a round-point manner to induce fibrosis and form a scleroderma model. This application can more comprehensively simulate the complex pathological process of immune abnormalities and tissue fibrosis coexisting in human scleroderma, and provides a more realistic and systematic experimental platform for in-depth exploration of the immune mechanism of this disease.

The application belongs to the technical field of data processing, and particularly relates to a data storage and visual processing system. The system comprises: a heterogeneous data acquisition component for multi-source data preprocessing and feature extraction; a secure distributed storage component for maintaining data integrity and synchronously resisting antigen information by using a hash linked list and a redundancy mechanism; an immune clone detection and analysis device for simulating an immune mechanism to recognize abnormalities through affinity matching and perform clone variation to accurately lock tampering areas; an adversarial generation evolution device for constructing a game defense mechanism to improve the detection accuracy and early warning capability for unknown attacks; and a dynamic defense visual rendering engine for assigning different visual coding parameters to different security level data areas according to the detection results. The application realizes the transformation from passive data display to active defense display by deeply coupling the immune adaptive mechanism and the adversarial generation network, and enhances the detection efficiency and visual feedback capability of the system for complex and dynamic variation attacks.

This invention relates to the field of biotechnology, specifically disclosing a method for constructing and applying a rare gudgeon strain susceptible to GCRV based on ISG58 knockout. The key technical points are as follows: This invention employs CRISPR / Cas9 gene editing technology to design dual-target sgRNAs targeting the ISG58 gene of rare gudgeon (target 1 induces a -2 bp deletion, and target 2 mediates an -8 bp deletion). The sgRNA sequence is optimized using ZiFiT target design software, and target specificity is ensured by NCBI BLAST alignment. The in vitro transcribed sgRNA and Cas9 protein are co-microinjected into rare gudgeon fertilized eggs to obtain F0 generation chimeras; after self-crossing of F1 generation heterozygotes, homozygous mutants conforming to Mendelian inheritance laws are successfully screened in the F2 generation. This invention, based on ISG58 knockout, obtains a rare gudgeon strain more sensitive to GCRV infection, which not only provides an important model for subsequent research on antiviral immune mechanisms in fish but also serves as an indicator organism for GCRV in aquaculture environments.

The application relates to the technical field of power regulation, and discloses a multi-mode coordinated control method and system based on a wind-solar-storage new energy power station, which comprises the following steps: acquiring real-time operation data of wind power, photovoltaic power generation and an energy storage system; predicting power grid load and wind power, photovoltaic power generation in a future period of time; recommending an energy scheduling scheme set by using a collaborative recommendation algorithm; determining an optimal energy scheduling scheme by using an improved Bayesian optimization algorithm based on an immune mechanism; and performing corresponding control operations on wind energy, light energy and energy storage equipment according to the obtained optimal energy scheduling scheme, so that efficient collaborative work of various energies is realized; and the application further discloses a multi-mode coordinated control system based on the wind-solar-storage new energy power station. The application can quickly find the optimal energy scheduling scheme, efficiently realize coordinated control of the wind-solar-storage new energy power station, better meet the power grid load demand, and maximize the energy utilization efficiency.

The invention is applicable to the technical field of biology, and provides a streptococcus suis type 2 multi-epitope inhalation type subunit vaccine and application thereof. According to the invention, 4 CTL epitopes, 7 HTL epitopes and 6 B cell epitopes are screened out aiming at conserved epitopes of SS2 virulence factors SSU05-1022 and SpaA, the epitopes are fused by AAY / GPGPG / EAAAK linkers, L7 / L12 ribosomal protein is introduced as an adjuvant, and the inhalation type subunit vaccine 1022-SpaA V3 is constructed; the vaccine can improve the levels of sIgA and serum IgG of respiratory mucosa and activate related immune cells by virtue of 60 micrograms of intranasal primary immunization-enhanced immunization, so that a mucosa and system dual immune mechanism is formed; the survival rate of mice after SS2 multi-strain challenge reaches up to 100%, and the bacterial load can be reduced; and the vaccine is safe and non-toxic, is inhaled, saves cost and is suitable for industrial large-scale production.

Provided is an Fc variant which has improved half-life by binding to and unbinding from FcRn in a pH-dependent manner, and which has improved selective binding to Fcγ receptors. Compared to a wild-type human antibody Fc domain and conventional antibodies approved as antibody therapeutic agents, the present human antibody Fc domain variants have a lower capacity to bind to immune-inhibiting receptors FcγRIIb and FcγRIIIb and have a higher capacity to bind to immune-activating receptor FcγRIIa (increased A / I ratio), thereby having a remarkably improved effector function and having maximized half-life in blood in which excellent pH-selective FcRn binding and unbinding capacity is exhibited, and thus bind to numerous peptide drug therapeutics having short half-life and retention time in the body so that long-term drug efficacy through increased blood half-life can be exhibited, and can maximize the immune mechanism of therapeutic protein drugs.

The invention discloses a basic group editing and monitoring system for gene linkage imbalance. The basic group editing and monitoring system comprises guide RNA (Ribonucleic Acid) designed for molecular targets obtained by screening transcription factor mediated gene linkage imbalance, a magnetic navigation-lipidosome nano-particle carrier and a reagent used by a space group imaging technology. According to the invention, magnetic navigation is combined with ferric oxide magnetic nanoparticles-lipid nanoparticles, so that the beneficial effects of improving editing targeting and efficiency and achieving accurate delivery are achieved; according to the method, multiple sites of the gene are edited at the same time, and overall intervention is carried out on the multiple sites of the gene linkage imbalance; the imaging system introduced by the invention can realize monitoring and spatial characterization of linkage imbalance, and is helpful for analyzing an immune mechanism.

The invention belongs to the technical field of biological agriculture, and particularly relates to application of a gene MOS4 and an encoding protein thereof to regulation and control of phytophthora parasitica resistance of plants. The gene function research on the gene MOS4 shows that the resistance of plants to phytophthora parasitica is remarkably improved by overexpressing the gene MOS4, the weakening of the phytophthora parasitica resistance of expression plants of the gene MOS4 is reduced, and the gene MOS4 is found to positively regulate the phytophthora parasitica resistance of the plants. The further study on the immune mechanism finds that the gene MOS4 regulates a salicylic acid (SA) signal channel related gene and interacts with a transcription factor ERF019 to jointly activate the plant immune response. In addition, a dual luciferase experiment proves that MOS4 and ERF019 cooperatively regulate and control transcription of PR1. The invention provides a new molecular target for preventing and treating plant parasitic phytophthora diseases, and has important agricultural application value.

The invention belongs to the technical field of biological agriculture, and particularly relates to application of a gene DISC1 and an encoding protein thereof to regulation and control of phytophthora parasitica resistance of plants. The functional research on the DISC1 and the encoded protein thereof finds that the gene DISC1 negatively regulates the resistance of plants to parasitic phytophthora. The over-expressed gene DISC1 significantly reduces the resistance of the plant to phytophthora parasitica, and the phytophthora parasitica resistance of the gene DISC1 mutant plant is significantly enhanced. The further study on the immune mechanism finds that the gene DISC1 regulates and controls the plant immune response by regulating and controlling the jasmonic acid (JA) signal channel related genes. The invention provides a new gene resource for preventing and treating parasitic phytophthora diseases of plants.

The invention discloses a method for regulating and controlling in-vitro programmed polarization of macrophages. Carrying out ultrasonic treatment on MXene to obtain MXene nanosheets, and adding the MXene nanosheets into a DMEM (Dulbecco Modified Eagle Medium) to obtain a regulation culture medium; the method comprises the following steps: culturing macrophages by using a DMEM culture medium until the macrophages are adhered to the wall, replacing the DMEM culture medium with a regulation culture medium, placing the macrophages in a rotating magnetic field generated by a rotating permanent magnet after the macrophages endocytose MXene nanosheets, enabling the MXene nanosheets to polarize the macrophages into M1-type macrophages through electromagnetic induction, and after the rotating magnetic field is removed, enabling the M1-type macrophages to become M2-type macrophages, the in-vitro program polarization of the macrophages is realized. The invention provides a simple method for establishing the macrophage polarization model, reduces the cost of establishing the model, improves the repeatability of a model experiment, and promotes related immune mechanism research.

This invention relates to the fields of artificial intelligence and big data processing, specifically disclosing a method for constructing high-dimensional user profiles based on multi-source data fusion and dynamic updates. This method constructs an initial high-dimensional feature vector, introduces an immune mechanism to identify "self" and "non-self" behaviors, establishes a dynamic antibody library with a lifecycle, and combines clonal selection, high-frequency mutation, and natural apoptosis mechanisms to achieve adaptive evolution of the profile. It also constructs a cross-user immune network, utilizes common group behaviors to generate regulatory factors to correct individual biases, and integrates adversarial attack detection, version management, and viability assessment modules. Through the above technical solutions, this invention improves the accuracy, timeliness, and anti-interference capability of user profiles, providing highly reliable data support for precision marketing, intelligent recommendation, and risk prevention and control.

The application discloses a kind of recombinant genes for increasing the protein expression level and expression length of co-transfected mRNA, and belongs to mRNA genetic engineering technology;The polypeptide encoded by the recombinant gene is a polypeptide with ribonuclease III activity, which is not inhibited by ribonuclease inhibitor and does not degrade single-stranded RNA;The recombinant gene and the target gene can be constructed in series or in parallel to form a fusion protein or an independently expressed recombinant protein;Or isolated by 2A peptide, internal ribosome entry site, etc., to express independent recombinant enhancement protein and target protein;The recombinant enhancement protein inhibits the innate immune mechanism and mRNA degradation mechanism of the host cell by degrading dsRNA, tRNA, miRNA and its precursor molecules, so that the protein expression level of co-transfected gene is increased by 3-4 times, and the expression time is prolonged by nearly 1 time;In addition, the present application inhibits the innate immune response to degrade impurities by rR3GE, and promotes the storage time of LNP preparation.

The application belongs to the technical field of biotechnology, and provides a Streptococcus suis type 2 (SS2) multi-epitope inhalation subunit vaccine and application thereof.Four CTL epitopes, seven HTL epitopes and six B cell epitopes are screened from the conserved epitopes of SS2 virulence factors SSU05-1022 and SpaA, and the four CTL epitopes, the seven HTL epitopes and the six B cell epitopes are fused by AAY / GPGPG / EAAAK linkers and introduced into L7 / L12 ribosomal protein as an adjuvant to construct an inhalation subunit vaccine 1022-SpaA V3.The vaccine is intranasally primed and boosted at 60 μg, can improve respiratory mucosa sIgA and serum IgG levels, activate related immune cells, and form a mucosal and systemic double immune mechanism.The survival rate of mice after SS2 multi-strain challenge is up to 100%, and the bacterial load can be reduced.The vaccine is safe and non-toxic, inhalation can save cost, and is suitable for industrial scale production.

Disclosed is polyethylene glycol (PEG)-modified IL-2, wherein the molecular weight of PEG is approximately 40 kD. Also disclosed are a use thereof in treatment of tumors, and a method for using PEG-modified IL-2 for treatment of tumors.

The invention is a composition for the treatment of HPV infections, which, with its components containing [3-caryophyllene, geranyl acetate, geraniol, and nerol, modulates the E7 and E2 protein pathways to provide a natural supportive therapeutic effect in managing HPV infections, exhibits antiviral, antibacterial, and immunomodulatory activity, significantly reduces viral replication, and suppresses protein expression, Contains Eugenia aromaticum (clove oil), which prevents viral replication by disrupting the structural integrity of cell membranes, exhibiting antimicrobial and antiviral activity with its high eugenol content, and Contains Cymbopogon martinii (palmarosa oil), which disrupts viral cell walls and inhibits the replication process, exhibiting antiviral and antibacterial properties with its high geraniol and citronellal content, and Contains Melaleuca viridiflora (niaouli oil), which strengthens cellular immune mechanisms to limit the intracellular spread of viruses and exhibits antiviral and immunomodulatory effects with its viridiflorol content.

The invention provides a power line communication self-healing method and system based on an immune mechanism, and the method comprises the steps: obtaining power line communication data, constructing a digital communication cell based on the power line communication data, obtaining the cell state data of the digital communication cell after communication transmission, the method comprises the following steps: constructing and training a communication immune model comprising a channel detection sub-module and a decoupling analysis sub-module, performing damage detection and root cause analysis on cell state data based on the communication immune model, generating a damage root cause analysis result, performing damage positioning according to the damage root cause analysis result, and performing damage healing based on a hierarchical collaborative self-healing mechanism. Therefore, the accurate positioning of the anomaly source and the rapid repair of the communication anomaly are realized.

Provided are Fc variants having improved half-life by binding to and unbinding from FcRn in a pH-dependent manner and which have improved selective binding to Fcγ receptors. The human Fc domain variants have lower capacity to bind to immune inhibiting receptor FcγRIIb and have higher capacity to bind to immune activating receptor FcγRIIa (increased A / I ratio) than a wild-type human antibody Fc domain and conventional antibodies approved as antibody therapeutic agents, thereby having remarkably improved ADCP induction ability and having maximized half-life in blood in which excellent pH-selective FcRn binding and unbinding capacity is exhibited, and thus bind to numerous peptide drug therapeutics having a low half-life and retention time in the body to enable the peptide drug therapeutics to have an increased blood half-life and exhibit long-term drug efficacy, and can maximize the immune mechanism of therapeutic protein drugs to be effectively used as an improved antibody drug.

The present disclosure provides an immunotherapeutic composition comprising (i) metallic cations (ii) an organic immunosuppressant (iii) phosphate species. The composition is a) capable of delivering antigens, b) activating immunological mechanisms in the immune cells c) inhibiting immune-suppressive cells in the body. The disclosure also provides a method of preparing said immunotherapeutic composition and a method of treatment of cancer and infectious diseases involving administration of said immunotherapeutic composition loaded with suitable antigens.