73 results about "Indirect elisa" patented technology

The invention discloses an indirect ELISA (enzyme-linked immuno sorbent assay) detection method of a porcine epidemic diarrhea virus antibody SIgA, and belongs to the technical field of animal antibody detection. In order to solve the problems of limited detection range and single immune evaluation dimension of a porcine epidemic diarrhea virus detection method in the prior art, the invention provides an indirect ELISA detection method of a porcine epidemic diarrhea virus antibody SIgA, and the detection method adopts a pichia pastoris eukaryotic secretion platform to prepare a high-activity PEDV S1 antigen; meanwhile, a secreting type IgA antibody is used as a detection target; the method has the advantages of high detection sensitivity, low background noise, better variant compatibility and practical popularization value, and provides a clinical and industrial-level solution for maternal immunity monitoring, piglet passive protection evaluation and vaccine immunity strategy optimization.

The invention discloses an EmAGO2 truncated recombinant protein, an echinococcosis multilocularis indirect ELISA (enzyme-linked immuno sorbent assay) diagnostic kit based on the truncated recombinant protein and a use method of the kit. The method comprises the following steps: designing a specific primer by selecting a sequence of 1-738bp at the N end of an Em-AGO2 gene, carrying out PCR (Polymerase Chain Reaction) amplification by taking reverse transcription echinococcosis multilocularis DNA (Deoxyribonucleic Acid) as a template, connecting an amplification product with a pCE2-TA / Blunt vector, and then transforming into a competent cell for culturing, so as to obtain a pCE2-T-EmAGO2 plasmid; respectively carrying out double enzyme digestion on the plasmid and an expression vector pET-28a (+), and connecting enzyme digestion fragments to obtain a recombinant protein expression plasmid pET-28a-EmAGO2; the preparation method comprises the following steps: taking EmAGO2 as a template, transforming the EmAGO2 into competent cells, selecting positive clones for induced expression, and carrying out ultrasonication treatment, separation and purification, concentration and desalination treatment on expressed thalli to obtain the EmAGO2 truncated recombinant protein. Furthermore, a mouse anti-EmAGO2 truncated recombinant protein and goat anti-rabbit IgG are respectively used as an antigen and an antibody to establish an indirect ELISA diagnostic kit for the echinococcosis multilocularis, and the kit has high sensitivity, specificity and good repeatability, and can be used for early diagnosis of the echinococcosis multilocularis.

The invention discloses a method for establishing indirect ELISA (enzyme-linked immunosorbent assay) for detecting mycobacterium bovis Lprl, which comprises the following steps: preparing mycobacterium bovis Lprl protein, taking the purified protein as an antigen, and determining conditions such as optimal antigen coating concentration, serum dilution, serum incubation time, secondary antibody dilution concentration, incubation time and the like. The established indirect ELISA detection method does not have cross reaction with other bovine pathogens, the specificity is good, the detection accuracy is high, and the total coincidence rate of test results of intradermal allergy with bovine tubercle bacillus is 95.8%; the intra-batch variation coefficient of the detected samples is 2.43%-3.61%, the inter-batch variation coefficient is 2.83%-3.96%, and the intra-batch variation coefficient and the inter-batch variation coefficient are both smaller than 10%. A new diagnosis target is developed, the established indirect ELISA method has the characteristics of high sensitivity, strong specificity, good repeatability and the like, the operation is easy, and a new method is provided for the detection work of the mycobacterium bovis.

The application belongs to the technical field of biology and particularly relates to an African swine fever virus pNP868R protein monoclonal antibody and a preparation method and application thereof. The application takes the pNP868R protein prepared by eukaryotic expression as an antigen, immunizes BALB / c mice, uses hybridoma technology, and obtains a hybridoma cell line capable of stably secreting the anti-African swine fever virus pNP868R protein monoclonal antibody through cell fusion, indirect ELISA method screening, limited dilution method subcloning and hybridoma continuous passage culture. The application also obtains an African swine fever virus pNP868R protein monoclonal antibody. The monoclonal antibody has high affinity with the pNP868R protein and can be applied in Western-Blot experiments, indirect immunofluorescence experiments, immunoprecipitation experiments and the capture of proteins interacting with pNP868R, and can be used for the diagnosis, prevention or treatment of African swine fever.

The application relates to a detection method of anti-preS1 antibody, an anti-preS1 antibody detection kit and application, the application carries out multi-sequence comparison and conservation analysis on preS1 sequences of different genotypes (A, B, C, D and CD mixed type) of HBV, takes type A (AB697487.1), type C (FJ032351.1) and type D (LC519808.1) as reference sequences, based on the sequence consistency standard of 85% of full length and 90% of the key region of functional antibody binding, a highly conservative preS1 full-length antigen sequence is systematically screened out in natural variation, the sequence can cover antigen variation of A-D type HBV, and the sequence is taken as a coating antigen to establish an indirect ELISA detection system, a good linear relationship is presented in the range of 4.88-625 ng / mL, and the Cut-off value is 33.6 ng / mL; clinical verification shows that the method can effectively distinguish chronic hepatitis B patients from healthy control groups, compared with detection methods based on partial fragments or overlapping short fragments, the false negative rate can be significantly reduced, and the method is suitable for HBV infection immune state evaluation, anti-virus curative effect monitoring and prognosis analysis.

The invention provides a fish creatine kinase sensitization and immune cross reactivity analysis method which comprises the following steps: preparing a fish muscle water-soluble protein crude extract, separating 40-45kDa protein components in the fish muscle crude extract through SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), and screening a target sensitization protein combined with IgE / IgG through immunoblotting; the method comprises the following steps: extracting total RNA of grass carp and synthesizing cDNA, designing a creatine kinase specific primer for amplification to obtain a creatine kinase gene sequence, and carrying out bioinformatics analysis after sequencing verification; the method comprises the following steps: cloning a creatine kinase gene to an expression vector, transforming escherichia coli BL21, performing IPTG induced expression, and performing urea gradient dialysis renaturation on an inclusion body to obtain the recombinant grass carp creatine kinase. Detecting the IgE binding activity of the recombinant grass carp creatine kinase and the serum of the allergic patient through dot hybridization; the IgG cross reactivity of the recombinant grass carp creatine kinase and the blue crab arginine kinase is verified through indirect ELISA; the IgE cross reaction degree of the recombined grass carp creatine kinase and the blue crab arginine kinase is quantified through inhibitory dot hybridization.

This invention discloses an indirect ELISA detection kit and method for bovine pasteurellosis, relating to the field of biotechnology. The indirect ELISA kit includes an enzyme-labeled plate coated with an antigen; the amino acid sequence of the antigen is shown in SEQ ID NO.2. The indirect ELISA detection kit constructed in this invention exhibits high specificity and sensitivity; the intra-assay coefficient of variation is between 1.79% and 7.54%, and the inter-assay coefficient of variation is between 2.23% and 6.91%, both below 10%, demonstrating good inter-assay and intra-assay repeatability. In testing 30 clinical samples, the overall concordance rate with the standard "Diagnostic Techniques for Bovine Hemorrhagic Sepsis (GB / T27530-2025)" was 96.67%. The indirect ELISA detection kit demonstrates good overall performance and can be applied to the detection of bovine pasteurellosis in clinical serum samples.

This invention relates to the application of the avian adenovirus serotype 4 multi-epitope recombinant chimeric protein rcF2 in the preparation of a kit for detecting avian adenovirus serotype 4 antibodies. The amino acid sequence of the avian adenovirus serotype 4 multi-epitope recombinant chimeric protein rcF2 is shown in SEQ ID NO.1. This invention utilizes the multi-epitope recombinant chimeric protein rcF2 to establish an indirect ELISA detection method and kit for detecting avian adenovirus serotype 4. This recombinant chimeric protein can be recognized by avian adenovirus serotype 4 positive sera, and the detection sensitivity of positive sera samples can reach 1:16000. It shows no cross-reactivity with antibodies against other pathogens, indicating that the ELISA detection method based on this multi-epitope recombinant chimeric protein rcF2 is simple to operate, enhances detection sensitivity, and has good specificity and repeatability.

This invention discloses anti-leptin monoclonal antibodies and their applications, relating to the field of biotechnology. This invention develops anti-leptin monoclonal antibodies 2A122H7 and 6B62E3, which can effectively recognize leptin. The ELISA kits prepared using these monoclonal antibodies 2A122H7 and 6B62E3 include indirect ELISA and double-antibody sandwich ELISA kits, exhibiting high specificity and sensitivity for the tested samples, and are convenient, efficient, and cost-effective. This invention provides effective technical support for advancing research on the regulatory mechanisms of economic traits such as feed intake, fat deposition, and reproduction in poultry.

The invention belongs to the technical field of biological medicine, and discloses an antibody for treating advanced rectal cancer and application thereof.The antibody is a monoclonal antibody 3C1 of targeted carcino-embryonic antigen (CEA), the sequence of a heavy chain variable region (VH) of the antibody is as shown in SEQ ID NO: 1, the sequence of a light chain variable region (VL) of the antibody is as shown in SEQ ID NO: 5, and the indirect ELISA detection titer reaches 1: 512000; also provided is an antibody conjugate (ADC) in which the antibody and raltitrexed are coupled by a linker, the drug-antibody ratio (DAR) being 3.0-4.0, the monomer purity being greater than or equal to 95%; the antibody conjugate (ADC) can be used for treating CEA positive advanced rectal cancer, animal experiments show that the tumor inhibition rate of ADC is 86% (significantly higher than 35% of free raltitrexed), the weight loss rate of mice is low, the toxicity of small intestines is light, the problems that existing drugs are poor in targeting and high in toxicity are solved, and the antibody conjugate has the advantages of high efficiency and safety.

The invention belongs to the technical field of bioengineering, and particularly relates to a preparation method and application of an N protein polyclonal antibody of a horse coronavirus. The polyclonal antibody is prepared by truncation expression of the horse coronavirus N protein, and a material is provided for research on inhibition of host innate immune mechanism by the horse coronavirus N protein. Prokaryotic expression is carried out on the N protein of the equine coronavirus, the polyclonal antibody is prepared, and a method and a material are provided for molecular research and mechanism research of the equine coronavirus. According to the invention, in combination with bioinformatics analysis of an N protein of the horse coronavirus, a pGEX-6p-N3 recombinant plasmid is constructed, and prokaryotic expression is carried out, so that a purified recombinant protein is obtained. After a mouse is immunized by the recombinant protein, a polyclonal antibody is obtained, an indirect ELISA method detects that the polyclonal antibody has good titer, Western Blot detection has good reactivity, and the polyclonal antibody can be applied to an indirect immunofluorescence test.

This invention constructs pET-32a-CP1 and pET-32a-CP2 expression vectors based on the CP1 and CP2 protein genes of Giardia duodenalis, transforms them into Rosetta (DE3) competent cells, and induces expression using IPTG. After SDS-PAGE analysis of the recombinant protein expression, Ni-NTA affinity chromatography is used to purify the proteins. Two indirect ELISA methods are then established using these as coating antigens. The indirect ELISA methods based on the recombinant proteins CP1 and CP2 of this invention exhibit good specificity, sensitivity, and repeatability, with concordance rates of 98% and 94% with microscopic detection results, respectively.

The invention belongs to the technical field of biology, and particularly relates to a recombinant monoclonal antibody of sheep anti-bluetongue virus VP7 protein. A sheep anti-bluetongue virus antibody library is obtained through a single cell V (D) J sequencing technology, a sheep anti-bluetongue virus VP7 protein recombinant monoclonal antibody is screened and successfully expressed, and it is identified through indirect ELISA and cellular immunofluorescence experiments that the recombinant monoclonal antibody has high affinity with bluetongue virus and can be specifically combined with VP7 protein. The recombinant monoclonal antibody is used as a competitive antibody to establish a competitive ELISA detection method aiming at the bluetongue virus VP7 protein, high specificity of bluetongue antibody detection can be realized, and a basis is provided for mastering the epidemiology of bluetongue virus and evaluating the immune effect of vaccines.

The invention discloses a CD147 nano antibody D2 as well as a preparation method and application thereof, and belongs to the technical field of molecular biology and immunology. The preparation method comprises the following steps: screening from an alpaca nano antibody phage library by utilizing a phage display technology, and sequencing to obtain 9 CD147 nano antibodies; 3 nano antibody genes are selected and cloned into pCold II, and prokaryotic expression recombinant plasmids are successfully constructed; the method comprises the following steps: carrying out inducible expression for 20 hours under the conditions that the final concentration of IPTG (isopropyl-beta-d-thiogalactoside) is 0.2 mmoL / L, the temperature is 16 DEG C and the speed is 100 rpm / min, carrying out nickel column purification, and then carrying out Coomassie brilliant blue dyeing and Western blot identification to determine and obtain the CD147 nano antibody which is named as C36, D2 and H4; the binding activity of the three nano antibodies and the CD147 protein is detected through indirect ELISA (enzyme-linked immuno sorbent assay). The result shows that the CD147 antigen and the three nano antibodies have binding activity and are dose-dependent. Under the same conditions, when the incubation concentration of the nano-antibody is as low as 1.25 micrograms / mL, the D2 binding effect is the best, the amino acid sequence of the nano-antibody is shown as SEQ ID NO.8, and the nucleotide sequence of the nano-antibody is shown as SEQ ID NO.10. The invention provides a more accurate method for detecting the CD147.

The invention discloses a CD147 nano antibody G45 as well as a preparation method and application thereof, and belongs to the technical field of molecular biology and immunology. A phage display technology is used for screening from a natural alpaca nano antibody phage library, finally three nano antibody genes are selected and cloned into pCold II, and prokaryotic expression recombinant plasmids are successfully constructed; the method comprises the following steps: carrying out inducible expression for 20 hours under the conditions that the final concentration of IPTG (isopropyl-beta-d-thiogalactoside) is 0.2 mmoL / L, the temperature is 16 DEG C and the speed is 100 rpm / min, carrying out nickel column purification, and then carrying out Coomassie brilliant blue dyeing and Western blot identification, so as to determine and obtain the CD147 nano antibody which is named as A8, B8 and G45; the binding activity of the three nano antibodies and the CD147 protein is detected through indirect ELISA (enzyme-linked immuno sorbent assay). The result shows that the CD147 antigen and the three nano antibodies have binding activity and are dose-dependent. Under the same conditions, when the incubation concentration of the nano-antibody is as low as about 0.625 g / mL, the G45 binding effect is good, the amino acid sequence of the nano-antibody is shown as SEQ ID NO.7, and the nucleotide sequence of the nano-antibody is shown as SEQ ID NO.10. The invention provides a more accurate method for high-sensitivity detection of CD147.

The invention belongs to the field of biology, and discloses a PDCoV recombinant RBD protein and an IgA Elisa kit thereof, and the amino acid sequence of the recombinant RBD protein is as shown in SEQ ID NO. 1. The recombinant RBD protein is used for an indirect ELISA kit, can cover IgA response in the early stage of infection, can accurately recognize a PDCoV specific IgA antibody in a breast milk sample, and cannot cause false positive due to infection of other common pathogens in a pig herd; the indirect ELISA kit is good in stability (the intra-batch variation coefficient is 1.848%-6.062%, and the inter-batch variation coefficient is 4.504%-7.790%), the coincidence rate of the indirect ELISA kit and an immunofluorescence experiment reaches 93.26%, the repeatability and the coincidence rate are high, and the indirect ELISA kit has the advantages of simplicity and convenience in operation, short time consumption and large detection flux of an ELISA method, and is suitable for large-scale screening of clinical pig milk samples.

Embodiments may include a rapid test device that provide rapid detection of substances, including those involved in pathogen infection, for example, using Microscale Affinity Chromatography (MAC), indirect ELISA, and optical molecular sensing technology. For example, in an embodiment, an apparatus may comprise a loading bay disposed on the apparatus to receive a cartridge, a door disposed on the apparatus to cover the loading bay, a plurality of prongs disposed on an interior of the door to provide actuation force to dispense blister reservoirs disposed on the cartridge when the door is closed, and a device disposed relative to the cartridge to move at least a portion of contents of the cartridge among chambers of the cartridge.

The invention relates to the technical field of virus detection, in particular to CCEV-99 recombinant protein, application of the CCEV-99 recombinant protein and an indirect ELISA detection method of a camel contagious pustular dermatitis virus antibody. In a kit used in the method, an ELISA plate is coated with CCEV-99 recombinant protein. The CCEV-99 recombinant protein can be used as a coating antigen of the camel contagious pustular dermatitis virus indirect ELISA detection kit, and rapid detection of a camel contagious pustular dermatitis virus specific antibody is realized; the detection method has the advantages of being good in specificity, sensitivity and repeatability, easy and convenient to operate, low in cost, capable of achieving large-scale detection and the like. The method is efficient, accurate and practical, and provides powerful technical support for follow-up research of camel contagious pustular disease.

The application discloses a detection method and a kit for detecting Listeria monocytogenes, and relates to the technical field of biology. The kit is an indirect ELISA kit, and comprises an enzyme label plate coated with an antigen. The amino acid sequence of the antigen is shown as SEQ ID NO. 2. The indirect ELISA detection kit constructed in the application only reacts with positive serum of Listeria monocytogenes, does not cross-react with other serum, and has high specificity. When the serum dilution degree is 1:800, the detection result is still positive, and the sensitivity is high. The batch variation coefficient is between 2.79% and 6.64%, the batch-to-batch variation coefficient is between 2.61% and 7.75%, and the batch-to-batch repeatability is good. For 24 serum samples, the total coincidence rate is 95.83%. The kit provided in the application can effectively detect sample infection of Listeria monocytogenes, and has the advantages of high specificity, high sensitivity and good repeatability.

The application discloses an indirect ELISA kit for rapidly detecting porcine epidemic diarrhea virus IgA antibody and application thereof and belongs to the technical field of immunology. The application provides a recombinant PEDV-RBD protein, and an amino acid sequence of the recombinant PEDV-RBD protein is shown as SEQ ID NO. 3. The application constructs an indirect ELISA kit for detecting porcine epidemic diarrhea virus IgA antibody based on the recombinant PEDV-RBD protein, can accurately capture PEDV IgA antibody in sow milk or serum to realize detection, has the advantages of high sensitivity and strong specificity, and provides a technical means for prevention and control of porcine epidemic diarrhea.

The invention relates to the technical field of medicine, in particular to a porcine circovirus type 3 Cap recombinant protein and a matched detection method and application thereof.According to the porcine circovirus type 3 Cap recombinant protein, efficient and high-quality preparation is achieved through gene optimization and expression process improvement, Escherichia coli rare codons in ORF2 genes are replaced with GeneOptimizer software, an unfavorable structure rich in arginine at the N end is removed, and the recombinant protein is obtained. The mRNA stability and the translation efficiency are obviously improved; through IPTG induction condition optimization and'inclusion body treatment-Ni-NTA affinity chromatography-dialysis renaturation 'purification processes, the final protein purity exceeds 95%, a high-quality material basis is provided for subsequent detection, the matched indirect ELISA detection method has the core characteristics of'accuracy, high efficiency and stability', detection can be completed within 2-3 h, synchronous analysis of large-batch samples is supported, and the detection efficiency is greatly improved.

Embodiments may include a rapid test device that provide rapid detection of substances, including those involved in pathogen infection, for example, using Microscale Affinity Chromatography (MAC), indirect ELISA, and optical molecular sensing technology. For example, in an embodiment, an apparatus may comprise a loading bay disposed on the apparatus to receive a cartridge, a door disposed on the apparatus to cover the loading bay, a plurality of prongs disposed on an interior of the door to provide actuation force to dispense blister reservoirs disposed on the cartridge when the door is closed, and a device disposed relative to the cartridge to move at least a portion of contents of the cartridge among chambers of the cartridge.

The application belongs to the field of biology, discloses PDCoV recombinant RBD protein and IgA ELISA kit thereof, and the amino acid sequence of the recombinant RBD protein is shown as SEQ ID NO.1.The recombinant RBD protein is used for indirect ELISA kit, can cover early IgA response of infection, can accurately recognize PDCoV specific IgA antibody in breast milk sample, and will not cause false positive due to infection of other common pathogens in a pig herd; the indirect ELISA kit of the application is stable (the batch variation coefficient is 1.848%~6.062%, and the batch variation coefficient is 4.504%~7.790%), and the coincidence rate with an immunofluorescence experiment reaches 93.26%, the repeatability and the coincidence rate are high, the method has the advantages of simple operation, short time consumption and large detection flux, and is suitable for large-scale screening of clinical pig milk samples.

The present invention belongs to the technical field of biological detection, and in particular to an indirect ELISA detection kit for infectious bovine rhinotracheitis virus (IBRV) antibodies and use thereof. Based on the good antigenicity of an IBRV gE protein, the present invention endows the indirect ELISA detection kit and an indirect ELISA detection method for IBRV, established based on the IBRV gE protein, with characteristics of sensitivity, specificity, and high efficiency. The method is simple to operate, time-efficient, and cost-effective, allowing for high-throughput detection of cattle infected with IBRV, and provides a simple and effective diagnostic tool for clinical diagnosis of infectious bovine rhinotracheitis (IBR).

The application discloses an ASFV dominant antigen epitope tandem recombinant protein, and an amino acid sequence of the ASFV dominant antigen epitope tandem recombinant protein is shown as SEQ ID NO:1, and belongs to the field of biology. The P54 and B602L proteins of the high immunogenicity region of the screened ASFV dominant antigen epitope are tandemly expressed according to the antibody induction efficiency in a pig group to obtain the recombinant protein. An ASFV indirect ELISA detection method is established by taking the recombinant protein as an antigen. The method has high accuracy, good specificity and high sensitivity, and has a large application prospect in the prevention and control of the ASF disease.

The application discloses a monoclonal antibody of a porcine rotavirus VP6 protein, an epitope of the monoclonal antibody and application of the monoclonal antibody. The monoclonal antibody can specifically recognize a linear B cell epitope on the VP6 protein, and a core sequence of the epitope is TTRLSFQLVR. The antibody has high affinity and high specificity, and can cross-react with porcine rotaviruses of various genotypes such as G5, G9 and G11. The application further includes an indirect ELISA detection kit containing the monoclonal antibody, and application of the linear epitope in detection of porcine rotaviruses, development of vaccines and research on functions of viral proteins. The application provides an important tool and theoretical basis for serological diagnosis, vaccine evaluation and etiological research of porcine rotaviruses.

The application discloses a PEDV ORF3 recombinant protein, which is encoded by a gene base sequence of a sequence table SEQ.ID.No.1 or has an amino acid sequence of SEQ.ID.No.2. The designed ORF3 recombinant gene can normally encode a protein, and the coded protein does not have a transmembrane domain. Researches show that the recombinant PEDV ORF3 recombinant protein has good antigenicity and immunogenicity, the antibody prepared based on the protein has high antibody titer and good specificity. Accordingly, the inventors also establish a corresponding indirect ELISA detection method, the method has the characteristics of strong specificity, good repeatability, high sensitivity and the like, can accurately identify PEDV, has the advantages of simplicity, rapidness, economy and the like, and the result is accurate, and the like, provides a new selection and reliable method for diagnosis of PED, and provides technical support for monitoring PEDV infection on a pig population. The clinical application prospect is good.

The application discloses an ELISA method for detecting porcine epidemic diarrhea virus antibody SIgA and belongs to the technical field of biology. The method comprises the steps of taking S1 protein as a coating antigen and detecting porcine epidemic diarrhea virus antibody SIgA in a sample to be detected, wherein the amino acid sequence of the S1 protein is a truncated sequence from the 19th to the 719th. The application preliminarily establishes an indirect ELISA method for detecting porcine epidemic diarrhea virus specific SIgA antibodies in serum, intestinal tissue and milk by optimizing conditions, and determines that the optimal antigen coating concentration and coating time are 0.25 mu g / ml and 4 DEG C overnight 12h-16h respectively, and the optimal detection conditions are determined. The method for detecting PEDV specific SIgA in serum, intestinal tissue and milk established in the research is simple and rapid, has good sensitivity and specificity, and provides a basis and a detection standard for evaluating the mucosal immune level of PEDV.

The invention discloses an anti-PRDX3 monoclonal antibody and application thereof, and belongs to the technical field of biological detection. The amino acid sequence of a heavy chain variable region of the antibody is SEQ ID NO.3, the coding gene is SEQ ID NO.1, the amino acid sequence of a light chain variable region is SEQ ID NO.4, and the coding gene is SEQ ID NO.2; the kit can specifically recognize a PRDX3 purified protein and a natural PRDX3 protein, and does not have cross reaction with a pET-28a empty vector irrelevant protein and a PRDX3 gene knockout sample; the antibody is matched with indirect ELISA (enzyme-linked immunosorbent assay), Western-blot, immunohistochemistry and other methods, and can qualitatively / quantitatively detect PRDX3 in various biological samples; the kit can also be used for preparing products for diagnosing fibrosis-related diseases, and provides a reliable tool for PRDX3 related research, disease diagnosis and clinical transformation by detecting PRDX3 expression in patient samples to assist diagnosis.

The application discloses application of a Toxoplasma gondii TGME49_229320 protein in preparation of a Toxoplasma gondii diagnosis kit, a nucleotide sequence of a coding gene of the Toxoplasma gondii TGME49_229320 protein is shown as SEQ ID NO:1, and the application further discloses a Toxoplasma gondii diagnosis kit and an indirect ELISA method for detecting Toxoplasma gondii for non-diagnosis purposes, and belongs to the field of molecular biology.The application provides more candidate antigens for pathogen detection and prevention and control of Toxoplasma gondii, and the established method can specifically detect antibody serum of Toxoplasma gondii oocyst infection, and has the advantages of good accuracy, strong sensitivity and the like.