221results about How to "Low instrument requirements" patented technology

The invention relates to a method for preparing 6- gingerol and 8- gingerol with fresh ginger. It is characterized in that it compries following steps: cutting fresh ginger into sheets, drying at low temperature, disintegrating, sifting with screen of 100 order, heating and refluxing and extracting with acetic ester or acetone, or extracting through immersion, extracting for three times, combining extract liquid, decompressing and recovering solvent, getting extract concretem dissloving it with acetic ester, treating it with chromatographic silica gel and washing it with benzinum purificatum acetic ester with gradient solvent, checking washing process by using TLC, decompressing to recover solvent and getting coarse 6- gingerol and 8- gingerol; washing coarse product with chloroform- carbinol solvent in Sephadex LH20 gel column, checking washing process with TLC, and getting washung liquid containing 6- gingerol and 8- gingerol with higher purity, decompressing to recover solvent and getting pure 6- gingerol and 8- gingerol, checking content with HPLC. The invention is characterized by simple operation, high productivity and production of two kinds of product at the same time.

A method for electrophoretic deposition preparation of the carbon nanotube film belongs to the field of the nano materials technology. The preparing method comprises the following steps: purification treatment and face finish for the carbon nanotube, the formation of the carbon nanotube stable suspension, electrophoretic deposition for the carbon nanotube film, film drying, and second electrophoretic deposition. The invention has the advantages of simple production equipment, rapid film forming speed, controllable film thickness, and unlimited substrate shape.

The invention discloses a method for preparing graphene by rotationally shearing in an electric field environment. Adopted experimental devices are a reaction container filled with electrolyte, high-purity graphite as a cathode, an inert electrode as an anode and a stirrer. The method comprises the following steps: switching on the cathode and the anode with a direct-current power supply, and stirring the electrolyte by using the stirrer; polarizing the cathode to strip the high-purity graphite under constant voltage, and simultaneously, stirring the electrolyte at a certain speed under the action of the stirrer, so that multi-layer graphene striped from the cathode is subjected to a shearing force again so as to prepare a solution containing graphene; cleaning the solution containing graphene for at least 5 times, ultrasonically dispersing, and centrifuging at a low speed; then, centrifuging a supernatant liquid of a prepared product at a high speed; and finally, drying to obtain graphene. The method disclosed by the invention is simple, convenient, rapid, safe, green and lower in cost; furthermore, natural graphene can be directly striped; due to the method, the traditional technology for preparing graphene by electrochemically striping is improved; and the yield and the quality for preparing a graphene film are increased.

The invention relates to a curcumin solid lipid nanoparticle with a P-gp inhibiting effect, and a preparation method and application thereof. The curcumin solid lipid nanoparticle comprises the following components according to mass ratio: 0.05-1% of curcumin, 5-15% of lipid material, 5-15% of an emulgator and the balance of water. According to the invention, the solid lipid nanoparticle technology is adopted to encapsulate curcumin, so that the stability of medicine is increased, the solubility of medicine is improved, the excretion function of small intestines is reduced, and the bioavailability of curcumin is improved. In addition, the preparation method of the curcumin solid lipid nanoparticle, disclosed by the invention, is the emulsification evaporation and low temperature solidification method which is simple and convenient and suitable for being used in a laboratory, and has low requirements for an apparatus.

The invention relates to a method for electrochemically depositing silver nanoparticles in a titanium dioxide nanotube array. The method comprises the following steps: (a) titanium sheet pretreatment: removing oil stain and impurities on the surface of a titanium sheet, burnishing the surface of the titanium sheet, carrying out polishing treatment, cleaning, and drying for later use; (b) preparing a TiO2 nanotube array on the surface of the titanium sheet by virtue of an anodic oxidation method; (c) deposition of silver nanoparticles: cleaning the titanium sheet on which the titanium dioxide nanotube array is formed on the surface for removing the impurities, then dipping the titanium sheet into mixed liquid of Ag ion-containing ethanol and water, connecting the titanium sheet to a cathode of a power supply, applying a voltage between the titanium sheet processed in the step (a) as an anode and the cathode, and electrochemically depositing silver; and (d) sample treatment: cleaning the sample prepared in the step (c), drying, and calcining. By virtue of the method, Ag nanoparticles can be deposited into a nanotube, so that the SPR effect of the silver nanoparticles can be effectively enhanced, and the photocatalytic performance of the TiO2 nanotube array can be further improved.

The invention discloses a method for preparing a high-yield SiC nanowire. The method comprises the followings steps of: performing mechanical mixing by taking 36.8 to 95.2 mass percent of industrial silicon powder or silicon dioxide powder and 4.8 to 63.2 mass percent of industrial expandable graphite as raw materials; filling a mixture into a graphite crucible and placing the graphite crucible into a high-temperature atmosphere furnace; filling argon as a protective gas after vacuumizing; raising a temperature to be between 1,300 and 1,700 DEG C at a speed of 2 to 15 DEG C/min; keeping the temperature for 1 to 8 hours; keeping the pressure intensity in the furnace lower than 1.0 MPa in an overall preparation process; naturally cooling to room temperature; and opening the furnace to obtain the SiC nanowire. The method has the characteristics of low-cost and easily-obtained raw materials, simple process without using a catalyst and productivity up to 65 percent, and is suitable for industrial production of the SiC nanowire.

The invention relates to a lily mottle virus (LMoV) double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) kit and a preparation method. The assay kit comprises a 96 micropore ELISA plate, an enzyme labeled antibody, a buffer solution, a developing solution and a stop solution, wherein the 96 micropore ELISA plate is coated with an LMoV specific polyclonal antibody, and the enzyme labeled antibody is an LMoV specific polyclonal antibody labeled by horse radish peroxidase. An immunogen used in the invention is a fusion recombinant antigen of LMoV coat protein and cytoplasmic inclusion protein, which is expressed by genetic engineering. The double-antibody sandwich enzyme-linked immunosorbent assay established by antibodies produced by the immunization can be used for detecting lily leaf specimens. Compared with other detection methods, the double-antibody sandwich enzyme-linked immunosorbent assay has high detection sensitivity and high accuracy, and ng-level viral antigenscan be detected. The preparation method of the kit is simple, convenient, quick, economical and practical.

The invention discloses an infrared-imaging detection method of high-altitude cirrus, and belongs to the field of false-alarm-source and target detection in remote-sensing and infrared-image processing. After an infrared image is acquired, firstly, preprocessing of the image is carried out, and target enhancement processing is carried out on a preprocessing result; then a region growing method isutilized to divide an enhanced portion into mutually disjoint blocks to complete coarse segmentation of the image; then the number of the blocks of coarse segmentation is used as a class number of clustering, clustering centers and a fuzzy membership matrix are initialized, and then positions of pixels relative to a center in the image is used, by a KFCM clustering method, as a basis of determination of an initial membership matrix to carry out clustering iteration to complete fine segmentation of the image; and finally, scattered isolated points/blocks are cleared for completing final segmentation, positions of the cirrus are determined, and a detection result is output. The method solves the problem that according to existing infrared-imaging technology, cirrus is difficult to accuratelydetect, detection efficiency is low, and detection speed is low.

The present invention mainly relates to a method for preparing metal base oxide gauze by utilizing layered layer nano assembly technique. Said method uses the nano oxide glue as adsorption deposition liquor and uses metal gauze as matrix to assemble to assemble to nano oxide on the metal gauze by using layered layer method under the action of static attraction. If the altermative adsorption of different kinds of oxide colloids is utilzied, the heterocomplexed metal base oxide gauze can be obtained. The prepared lattice surface ultrathin oxide film can provide quick diffusion and high active position utilization rate. The high heat conductivity of metal basis is suitable for exothermal reaction, and its unique gauze structure more possesses superiority in mass transfer field, and is convenient for operation. Said invention method can be extensively used in catilisis, separation industry and environmental protection and preparation of sensor and modification of electrode.

The invention relates to an LIBS and Raman spectrum aerosol online detection device based on a single particle. An aerodynamic lens is used for focusing an atmospheric aerosol particle sample into a collimated particle beam and screening a particle size, and an oscilloscope, two continuous lasers and corresponding photomultipliers jointly form a double-beam diameter measuring system. And a first Nd: YAG laser and a second Nd: YAG laser act with particulate matters to excite a Raman spectrum and an LIBS spectrum. Element composition information in aerosol particles can be detected in real timethrough the LIBS spectrum, characteristic spectral lines of all elements belong to atomic spectral lines, and overlapping interference is avoided. The Raman spectrum studies vibration and rotation spectrum of molecules to obtain structure information of aerosol particles, and obtains lattice vibration information of a particle sample. The device is simple and compact in structure and low in instrument requirement, background noise interference is eliminated only through a low-vacuum environment, and cost of the detection device is reduced. The device is short in detection process, and an analysis result can be obtained after a single pulse laser.

The invention discloses a method for testing cable main insulator water tree phenomenon initial points. The invention comprises starting from an angel of a condition that conductible ions are precipitated in a cable semi conductive shielding layer, imitating practical working hot and humid environment of the cable semi conductive shielding layer, characterizing the cable main insulator water tree phenomenon initial points. After sample hot water is cooked, a mathematical model is established, the mathematical model analyzed, a density distribution of the conductible ions which are in a t time sample is obtained, a precipitating condition of the conductible ions in the cable semi conductive shielding layer can be described well, which is used to analyze effects of the cable semi conductive shielding layer to the water tree phenomenon initial points which is produced through a cable main insulating layer. The testing method has the advantages that the requirement for needing equipment is low, tests can be advanced under normal lab conditions, and testing cost can be lowered by 10%. After data base is built successfully, the invention can be moved out immediately if the invention is needed without testing surface smoothness, which saves testing time.

The invention belongs to the field of medicines, and relates to an application of a method for degrading all carbohydrate chains in the blood to be monosaccharide and detecting the monosaccharide in cancer detection. A sample in the method for degrading the blood to obtain the monosaccharide and detecting the monosaccharide is the blood, and the cancer includes lung cancer, gastric cancer, ovarian cancer, penis cancer, esophagus cancer, oral cancer, biliary duct cancer, breast cancer, adenocarcinoma perampullaire, rectal cancer and bladder cancer. The method has the characteristics of simplicity and easiness in operation steps, easiness in popularization, short detection time, low requirement on the instrument, low detection cost, less consumption of blood and the like. The result displays that based on the content of eight monosaccharides in the blood, not only can a normal person and a cancer patient be distinguished, but also different cancers can be distinguished. The method for simplifying glycomics knowledge and applying in the detection of the biomarker of blood is first created in the world.

The invention provides a phosphorus-doped bimetallic selenide electrocatalyst material with an adjustable monometallic element electronic structure as well as a preparation method and application of the phosphorus-doped bimetallic selenide electrocatalyst material. The bimetallic selenide electrocatalyst material is obtained by carrying out hydrothermal treatment on the surface of foamed nickel to grow nickel-iron hydrotalcite nanosheets and then carrying out selenylation and phosphorus doping. According to the method, introduced phosphorus atoms can independently regulate and control the electronic structure of the metal element iron on the premise that the electronic structure of nickel is not changed, so the electrocatalytic activity of the material is further influenced, and it is indirectly proved that the electronic structure of the element iron has great influence on the catalytic activity. In addition, a material source is wide, a synthesis method is simple and convenient, and requirements on equipment are low; and the obtained material is used for an electrochemical oxygen evolution reaction, and has electrochemical properties such as ultrahigh stability and ultralow overpotential.

The invention discloses a fluorescence probe and a synthetic method thereof and an application of the fluorescence probe in circulating tumor cell detection. The fluorescence probe comprises a fluorescence group, a cathepsin B substrate and folic acid molecules, the fluorescence group is connected with the cathepsin B substrate through a self-degradation bond, and then is connected with the folic acid molecules; the fluorescence group is excited by the excitation light under a dissociation state, and then is enable covalent connection with other molecules for quenching. The fluorescence probe is the switching-mode fluorescence probe having a spontaneous cascade amplification function, can be used for avoiding complex operation steps of antibody or nucleic acid detection during a circulating tumor cell detection process, is used for an automatic biochemical analyzer as a matching reagent, is in favor of promoting automation detection of tumor cells, and has the advantages of simple and convenient operation, short time consuming, and low apparatus requirement. The fluorescence probe can be used for positioning minimal tumor cells and tubercle in clinical operation, and has wide application prospect and large market value.

The invention discloses LAMP (loop-mediated isothermal amplification) primers, a kit and a method for detecting murine klebsiella pneumoniae. A visual detection method of murine klebsiella pneumonia which is suitable for clinical sample detection and has strong specificity and high sensitivity is established by designing the LAMP primers by virtue of a klebsiella pneumoniae tyrB gene sequence. Whether klebsiella pneumoniae exists or not can be detected quickly and accurately by observing whether green fluorescence exists or not, and the lowest detectable limit is 5.6 copy number/ reaction. According to identification of a specific sequence region of a target sequence by four primers designed and screened, high specificity of LAMP diffusion is guaranteed, so that the LAMP primers are suitable for field application at basic level.