Preparation method of Curdlan oligomers

A technology of oligosaccharides and polysaccharides, which is applied in the field of degrading codlan polysaccharides to prepare codlan oligosaccharides by acid method, can solve the problem that it is not conducive to the study of oligosaccharide structure and biological activity function, and the technology of codran oligosaccharides is difficult, difficult and Cost prohibitive and other issues, to achieve the effect of enhancing natural immunity, inducing plant disease resistance, and promoting environmental friendliness

Inactive Publication Date: 2012-01-11
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the chemical synthesis of cotlan oligosaccharides is difficult, especially the synthesis of oligosaccharide monomers with DP>7, because it involves a series of problems such as the connection mode and three-dimensional configuration of sugar units, and the protection of active groups. and cost are prohibitive
[0005] If there is no effective method for preparing Cortlan oligosaccharides, it will not be conducive to the study of the structure and biological activity of oligosaccharides, and it will hinder the in-depth exploration of the relationship between Cortlan oligosaccharides and their derivatives and the occurrence of diseases from the perspective of mechanism

Method used

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  • Preparation method of Curdlan oligomers
  • Preparation method of Curdlan oligomers
  • Preparation method of Curdlan oligomers

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Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1: the preparation method (A) of cotlan oligosaccharide

[0022] Weigh 5g of candlan polysaccharide in 50ml of potassium iodide and cadmium ethylenediamine solution (ratio: 1:1) and stir well until completely dissolved, slowly add 0.5 M oxalic acid mixed solution 3ml, reacted for 60min, precipitated with 50mL of ethanol and acetone mixed solution (V / V=1), while stirring, to obtain milky white powder, suction filtered, and vacuum dried at 50°C to obtain 4.25g, yield up to 85.12%.

[0023] The obtained substance was proved to be Cortlan oligosaccharide by detection by high performance liquid chromatography and matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

Embodiment 2

[0024] Embodiment 2: the preparation method (B) of cotlan oligosaccharide

[0025] Weigh 5g of candlan polysaccharide in 50ml of dimethyl sulfoxide and potassium iodide solution (ratio: 1:1), stir well until completely dissolved, and slowly add 3ml of 0.5M trifluoroacetic acid mixed solution, reacted for 60min, neutralized with mixed alkali solution, neutralized with mixed alkali solution, concentrated to 10ml by rotary evaporation at 45°C (can be operated at 30-150°C), and was light The yellow transparent liquid was precipitated with 50mL of a mixed solution of ethanol and acetone (V / V=1) while stirring to obtain a milky white powder, which was filtered by suction and vacuum-dried at 50°C to obtain 4.51g of cotlan oligosaccharides, with a yield of 90.2 %.

[0026] The raw material of the present invention candlan polysaccharide is so far the only β-1,3-glucan found in nature that does not contain branched chain structure, which is different from other glucans containing bran...

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Abstract

The invention discloses a preparation method of Curdlan oligomers. The method comprises the following steps of: fully dissolving Curdlan polysaccharide into a solvent to obtain a homogeneous solution; adding acid at a temperature in a range of 80-140 DEG C, wherein a final concentration of the acid in the solution is 0.1-10 M; reacting for 30-500 min; adding organic solvent sediments which are 3-5 times as much as the volume of reaction liquid and meanwhile, stirring and filtering to obtain a milky solid; washing the solid by the organic solvent 1-4 times to obtain the Curdlan oligomers with an average molecular weight of 340-4000 Da, which is measured by an efficient liquid phase detection after drying. The degradation efficiency of the preparation method reaches more than 85%. The Curdlan oligomers are oligomers formed by connecting glucose molecules through beta-(1->3) glucosidic bonds; the degree of polymerization (Degree of Polymerization, DP) of the Curdlan oligomers is 2-24; a molecular formula of the Curdlan oligomers is (C6H12O6)n, wherein n is equal to 2-24. The average molecular weight of the Curdlan oligomers is 340-4000 Da. The preparation method provided by the invention has a simple and controllable preparation process, short reaction time and good degradation efficiency, and is environmentally friendly and suitable for industrial production.

Description

technical field [0001] The invention relates to codlan oligosaccharides, in particular to a method for preparing codlan oligosaccharides by degrading codlan polysaccharides with an acid method. Background technique [0002] Cordlan polysaccharide is an exopolysaccharide produced by the fermentation of Alcaligenes faecalis var myxogenes 10c3. Its basic structure is glucose connected by β-(1→3) glycosidic bonds. It is a member of the β-1,3-glucan family member. So far, this polysaccharide is the only non-branched β-1,3-glucan in nature. However, its application in food, medicine, feed additives and materials is seriously hindered due to its insolubility in water. [0003] Cotlan oligosaccharides, also known as β-1,3-glucooligosaccharides, are degradation products of polysaccharides, and their water solubility is greatly enhanced. As the research on oligosaccharides has attracted more and more attention, the biological functions and physiological activities of cotlan oligosa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C13K13/00C08B37/02
Inventor 杜昱光傅赟彬刘启顺赵小明白雪芳李曙光
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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