The invention discloses an incision beta-1,3-glucanase coding gene from cellulosimicrobium cellulans, as well as enzyme, a preparation method and application of the incision beta-1,3-glucanase coding gene. The incision beta-1,3-glucanase coding gene is cloned to an escherichia coli expression vector through a technical method of gene engineering, so that an escherichia coli recombination strain capable of realizing heterologous expression of the enzyme can be obtained; incision beta-1,3-glucanase prepared through the heterologous expression of the strain can directly and efficiently degrade solid Curdlan. The invention further provides an antibiological inoculant, which is the incision beta-1,3-glucanase, for preventing and treating animal and plant diseases; the antibiological inoculant belongs to the field of biological prevention and treatment, and is expected to be novel biological pesticide. The incision beta-1,3-glucanase provided by the invention can be widely applied to the field of agriculture, food, feed additives, medicines, glucooligosaccharides preparation and the like.