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Rhizobium radiata and method for producing curdlan gum fermentation liquid using the bacterium

A technology of Rhizobia radiata and fermentation broth, applied in the field of microbial fermentation, can solve problems such as low solid content

Active Publication Date: 2011-12-14
HEBEI XINHE BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional process of curdlan gum preparation is to produce strain (bacterium strain uses Agrobacterium Agrobacterium biovar1 ) is inoculated in a sterile medium with sucrose as the main carbon source for fermentation, temperature, pH and other process conditions are controlled, and the fermentation is completed to obtain curdlan gum fermentation liquid. The above-mentioned prior art has the prepared curdlan gum product Defects of low solids content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The present embodiment comprises the following process steps:

[0059] A, the seed liquid containing the rhizobia radiata CGMCC NO.5031 after the expansion is inserted in the culture liquid of the fermenter according to the inoculation amount of 5% according to the mass percentage, contains carbon source, necessary nitrogen source and Nutrients;

[0060] B, adjust the pH of fermentation medium, carry out aerated fermentation;

[0061] C. Add carbon source, nitrogen source, nutrients and water during the fermentation process, and obtain a fermented liquid containing curdlan through not less than 90 hours of aerated fermentation.

[0062] The fermentation culture liquid in described step A is made up of the component of following mass percentage:

[0063] Glucose 6%, sodium nitrate 0.3%, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.15%, FeSO 4 10ppm, biotin 20ppm, polyether defoamer 0.03%, the balance is sterile water; the pH of the fermentat...

Embodiment 2

[0088] The present embodiment comprises the following process steps:

[0089] A, will contain the seed liquid of rhizobia radiata CGMCC NO.5031 after expanding cultivation and insert in the culture liquid of fermenter according to the inoculum amount of 10% according to mass percentage, contain carbon source, necessary nitrogen source and Nutrients;

[0090] B, adjust the pH of fermentation medium, carry out aerated fermentation;

[0091] C. Add carbon source, nitrogen source, nutrients and water during the fermentation process, and obtain a fermented liquid containing curdlan through not less than 90 hours of aerated fermentation.

[0092] Concrete technical solution of the present invention also has:

[0093] The fermentation culture liquid in described step A is made up of the component of following mass percentage:

[0094] Glucose 8%, sodium nitrate 0.45%, dipotassium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.3%, FeSO 4 15ppm, biotin 30ppm, polyether...

Embodiment 3

[0119] The present embodiment comprises the following process steps:

[0120] A, will contain the seed liquid of rhizobia radiata CGMCC NO.5031 after expanding cultivation and be inserted in the culture liquid of fermenter according to the inoculum amount of 8% according to mass percentage, contain carbon source, necessary nitrogen source and Nutrients;

[0121] B, adjust the pH of fermentation medium, carry out aerated fermentation;

[0122] C. Add carbon source, nitrogen source, nutrients and water during the fermentation process, and obtain a fermented liquid containing curdlan through not less than 90 hours of aerated fermentation.

[0123] Concrete technical solution of the present invention also has:

[0124] The fermentation culture liquid in described step A is made up of the component of following mass percentage:

[0125] Glucose 7%, sodium nitrate 0.4%, dipotassium hydrogen phosphate 0.15%, potassium dihydrogen phosphate 0.2%, FeSO 4 12ppm, biotin 25ppm, polye...

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Abstract

The invention belongs to microbial fermentation, and particularly discloses Rhizobium radiobacter and a method for producing curdian fermentation liquor by using the strain. The method for producing the curdian fermentation liquor by using the strain comprises the following steps of: inoculating seed liquid which contains Rhizobium radiobacter CGMCC No.5031 serving as the strain after amplification culture into culture solution of a fermentation tank according to the inoculation amount of between 5 and 10 mass percent, wherein the fermentation culture solution contains a carbon source, an essential nitrogen source and nutrient substances; regulating the pH value of the fermentation culture solution, and performing ventilation fermentation; and supplementing a carbon source, a nitrogen source, nutrient substances and water in the fermentation process, and performing ventilation fermentation for not less than 90 hours to obtain fermentation liquid containing curdian. By the method, the defect that the content of solid matter in the curdian fermentation liquor is low in the prior art is overcome; and the method has the advantages that: the carbon source in the fermentation liquor can be better utilized by the strain, the transformation rate is high in the fermentation process, the content of the solid matter in the fermentation liquor is high, the production cost is greatly reduced and the like.

Description

technical field [0001] The invention belongs to microbial fermentation, in particular to rhizobia radiata and a method for producing curdlan fermentation liquid by using the bacteria. Background technique [0002] Curdlan gum is a new type of microbial exopolysaccharide. Because the polysaccharide has the unique property of forming a gel under heating conditions, it is also called thermal gel. The English name is Curdian. It can be widely used in food, medicine and other industries. . The traditional process of curdlan gum preparation is to produce strain (bacterium strain uses Agrobacterium Agrobacterium biovar1 ) is inoculated in a sterile medium with sucrose as the main carbon source for fermentation, temperature, pH and other process conditions are controlled, and the fermentation is completed to obtain curdlan gum fermentation liquid. The above-mentioned prior art has the prepared curdlan gum product Defects with low solids content. Contents of the invention [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/41
Inventor 张禹张国佩张少华刘学珍
Owner HEBEI XINHE BIOCHEM
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