The invention discloses a preparation method of
wheat gluten protein peptide with low bitterness and high XOD inhibitory activity, which comprises the following steps: by taking wet
gluten as a
raw material, sequentially carrying out enzymolysis by adopting alpha-
amylase and glucamylase to remove
starch, then carrying out high-activity enzymolysis on the washed enzymatic
hydrolysate by utilizing
trypsin, and adding flavourzyme to further regulate and control
flavor, so as to obtain the
wheat gluten protein peptide with low bitterness and high XOD inhibitory activity. And finally, adjusting the pH value of the enzymatic
hydrolysate to high
alkalinity, and reducing the aggregation of insoluble
peptide aggregates in combination with ice-bath
ultrasound. The technical problem that low bitterness and XOD inhibitory activity in
wheat gluten protein enzymolysis products are difficult to consider at the same time is successfully solved, the proportion of active peptide fragments with the molecular weight smaller than 500 Da in the prepared wheat peptide is larger than 45%, the
xanthine oxidase inhibitory activity is larger than 90%, and the bitterness value is lower than 3.5. The enzymolysis effect is greatly improved, the
energy consumption is saved, the production cost is reduced, the product profit is improved, and the method can be widely applied to development of
uric acid reducing
functional food, health care products or medicines.