Method for preparing foliage fertilizer containing nucleic acid degradation product

A nucleic acid degradation and foliar fertilizer technology, which is applied in the direction of organic fertilizers, can solve the problems of no nucleic acid degradation products in the sales of foliar fertilizers, single components of nucleic acid degradation products, and unfavorable environmental protection, etc., to improve wheat quality and ecology Obvious effect, good soil and environment effect

Inactive Publication Date: 2008-05-28
MICROBIOLOGY INST OF SHAANXI
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Problems solved by technology

Chinese patent document (CN1238323A) discloses a kind of preparation method of nucleic acid foliage fertilizer, because the degradation of its nucleic acid adopts the chemical method of alkaline hydrolysis, makes the nucleic acid degradation product only have purine and pyrimidine, and component is single; Therefore, this patent exists Nucleic acid degradation products have a...

Method used

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Examples

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example 1

[0017] Example 1. Take 2.5kg of beer production leftover malt root and put it into 25L of water, stir evenly, soak for 12 hours at 4°C, filter and let the filtrate stand for 45 minutes to collect the supernatant as nuclease solution. The amount of enzyme solution is about 16L, preheated to 60°C;

[0018] Dissolve 1 kg of ribonucleic acid (yeast ribonucleic acid produced by Shanghai Bozhong Company) with stirring in 25 L of water at 60°C, adjust the pH to 5.0 with KOH solution; Hydrolyze at 65°C for 3.3 hours; after hydrolysis, immediately heat and boil for 10 minutes to inactivate the enzyme, adjust the pH to 7.0 with KOH, remove incompatible impurities by centrifugal filtration at 3500r / min, collect the supernatant and concentrate under vacuum to 6.5L (the content of nucleic acid hydrolyzate is about 104g / L); through drying and dusting, 1.54kg of finished foliage fertilizer is obtained, wherein the content of nucleic acid hydrolyzate is 42%.

example 2

[0019] Example 2: Take 2.5kg of beer production leftover malt root and put it into 25L of water, stir evenly, soak for 10 hours at 10°C, filter and let the filtrate stand for 40 minutes to collect the supernatant as nuclease solution, the amount of enzyme solution is about For 18L, preheat to 60°C;

[0020] Dissolve 1 kg of ribonucleic acid (yeast ribonucleic acid produced by Shanghai Bozhong Company) under stirring with 30L water at 70°C, and adjust the pH to 5.0 with KOH solution to aid dissolution; mix the enzyme solution at 60°C with the nucleic acid solution, adjust the pH to 5.6, and Hydrolyze at 65°C for 3.8 hours; after hydrolysis, immediately heat and boil for 10 minutes to inactivate the enzyme, adjust the pH to 7.0 with KOH, remove incompatible impurities by centrifugal filtration at 3800r / min, collect the supernatant and concentrate under vacuum to 6.8L (Nucleic acid hydrolyzate content is about 97g / L); Through vacuum low-temperature drying, 1.62kg of foliar fertil...

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Abstract

The invention relates to a preparation method of a foliar fertilizer which comprises nucleic acid degradation products. The invention is characterized in that: malt sprout is employed to obtain nuclease liquid with a low temperature water extraction method, nucleic acid solution and enzyme solution are blended according to a proportion by weight of 1 : 2 to 3 portions of nucleic acid and malt sprout, and the solution is hydrolyzed at a temperature of 65 DEG C and a condition of pH 5.6 for 3 to 4 hours, then a nucleic acid degradation product foliar fertilizer is obtained after enzyme dispelling, filtering, enzyme and milling. The preparation method of the invention is characterized by simple technique, easily available raw material, pollution free exhaust and low production cost; the prepared nucleic acid degradation products of the foliar fertilizer are nucleosides, nucleotides, pyrimidine, purine and other micromolecule, thus being beneficial to the metabolism process of nucleic acid, and the invention also contains a great amount of nutrient components such as glucide, protein and minerals, etc., which are needed by the growth of crops, thus promoting the growth of crops, strengthening resistance and obviously improving yield and quality; the yield of wheat is improved by 7 to 10 percent, protein content is improved by 1 percent and wheat gluten content is improved by 3 percent; solution is prepared by the foliar fertilizer of the invention according to requirements of the growth of crops to spray; absorption speed of foliage is fast, and brings no pollution to soil; the foliar fertilizer is a non polluted green fertilizer.

Description

technical field [0001] The invention belongs to the improvement of the preparation method of foliage fertilizer containing nucleic acid degradation products, and specifically relates to the technical field of preparation of crop growth fertilizer. Background technique [0002] Nucleic acid is the basic substance of life and the basis of material metabolism and energy metabolism. Differences in the nature and quantity of nucleic acid can lead to the diversity of biological species and growth states. For the same kind of living organisms, the difference in the amount and form of nucleic acid determines the strength of the living organisms. Therefore, nucleic acid substances play an irreplaceable role in increasing crop production and improving product quality; small nucleic acid molecules such as nucleosides, nucleotides, pyrimidines, and purines can not only regulate the growth of plants, but also be used as a source of nutrition by plant leaves. It can be quickly absorbed b...

Claims

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Application Information

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IPC IPC(8): C05F11/00
Inventor 慕娟问清江党永吴小杰李叶昕
Owner MICROBIOLOGY INST OF SHAANXI
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