Method for preparing foliage fertilizer containing nucleic acid degradation product
A nucleic acid degradation and foliar fertilizer technology, which is applied in the direction of organic fertilizers, can solve the problems of no nucleic acid degradation products in the sales of foliar fertilizers, single components of nucleic acid degradation products, and unfavorable environmental protection, etc., to improve wheat quality and ecology Obvious effect, good soil and environment effect
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example 1
[0017] Example 1. Take 2.5kg of beer production leftover malt root and put it into 25L of water, stir evenly, soak for 12 hours at 4°C, filter and let the filtrate stand for 45 minutes to collect the supernatant as nuclease solution. The amount of enzyme solution is about 16L, preheated to 60°C;
[0018] Dissolve 1 kg of ribonucleic acid (yeast ribonucleic acid produced by Shanghai Bozhong Company) with stirring in 25 L of water at 60°C, adjust the pH to 5.0 with KOH solution; Hydrolyze at 65°C for 3.3 hours; after hydrolysis, immediately heat and boil for 10 minutes to inactivate the enzyme, adjust the pH to 7.0 with KOH, remove incompatible impurities by centrifugal filtration at 3500r / min, collect the supernatant and concentrate under vacuum to 6.5L (the content of nucleic acid hydrolyzate is about 104g / L); through drying and dusting, 1.54kg of finished foliage fertilizer is obtained, wherein the content of nucleic acid hydrolyzate is 42%.
example 2
[0019] Example 2: Take 2.5kg of beer production leftover malt root and put it into 25L of water, stir evenly, soak for 10 hours at 10°C, filter and let the filtrate stand for 40 minutes to collect the supernatant as nuclease solution, the amount of enzyme solution is about For 18L, preheat to 60°C;
[0020] Dissolve 1 kg of ribonucleic acid (yeast ribonucleic acid produced by Shanghai Bozhong Company) under stirring with 30L water at 70°C, and adjust the pH to 5.0 with KOH solution to aid dissolution; mix the enzyme solution at 60°C with the nucleic acid solution, adjust the pH to 5.6, and Hydrolyze at 65°C for 3.8 hours; after hydrolysis, immediately heat and boil for 10 minutes to inactivate the enzyme, adjust the pH to 7.0 with KOH, remove incompatible impurities by centrifugal filtration at 3800r / min, collect the supernatant and concentrate under vacuum to 6.8L (Nucleic acid hydrolyzate content is about 97g / L); Through vacuum low-temperature drying, 1.62kg of foliar fertil...
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