46results about How to "Low nutritional needs" patented technology

The invention discloses an environmentally-friendly soft-shelled turtle compound feed, including the following material components (each component is in the air-dry substance weight percentage): 30-35% of white fish meal, 15-25% of red fish meal, 5-10 % of fermented soybean mea, 3-6% of extruded soybean, 1-4% of wheat gluten, 5-10% of beer yeast, 18-25% of pre-gelatinized starch, 1-2% of calcium phosphate, 0.05-0.3% of enzyme preparation, 0.1-0.4% of microbial agents, 0.1-0.3% of compound multivitamins, 0.5-1.5% of composite multi-ore, 0.2-0.5% of 50% choline chloride. The inventive prescription can reduce the nitrogen and phosphorus content in the compound feed, improve the digestive absorption and deposition rate of the nitrogen and phosphorus in the soft-shelled turtle breeding, and can reduce the output of nitrogen and phosphorus in water to improve the breeding environment.

The invention discloses a preparation method and application of pseudomonas chlororaphis for resisting disease and promoting growth. A strain is pseudomonas chlororaphis HT66CCTCC No:M2013467. A main metabolite of the strain comprises phenazine-1-phenazine and phenazine-1 formamide. The strain is inoculated into a microorganism culture medium and is fermented to obtain a thallus with high concentration and the metabolite, and can be mixed with materials like powder active carbon, bentonite, swell soil, kaolin, diatomite, zeolite, calcium carbonate and the like to prepare a microbial agent; the quantity of viable organisms in a finished product is 1-8.5*10<9>cfu / mL. The tablet laboratory verifies that the pseudomonas chlororaphis has good control effect of pathogenic bacteria of rice sheath blight diseases, cotton fusarium wilt, gaeumannomyces graminis and watermelon fusarium wilt, and also has a good promotion function in the growth of a part of plants.

The invention discloses a gene engineering bacterial strain used for producing phenazine-1-methanamide and its purpose; which is characterized in that knockout of psrA or rpeA gene in Pseudomonas chlororaphis HT66(Pseudomonas chlororaphis HT66) CCTCC NO: M 2013467 genome is carried out. According to the invention, a strain of Pseudomonas chlororaphis is performed with seed culture, fermentation, extraction, concentration and crystallization to obtain phenazine-1-methanamide with purity of more than 96%. After the deletion of psrA and rpeA gene in the bacterium genome, the fermentation output of phenazine-1-methanamide can reach as high as 1800ppm. The agar plate bacteriostasis tests found that phenazine-1-methanamide has obvious inhibition effect on common plant pathogenic fungi such as Rhizoctonia solani, fusarium oxysporum, pythium wltmum and Stevia Septoria, has good stability and activity, and has good application prospect.

The present invention is directed to isolating the valuable components of cereal grains and allowing the benefits to be more fully exploited. The concept of the present invention initially involves selection of cultivars of cereal grains such as wheat, barley, oats and rye having desired bioactive components including antioxidants, complex phenolics, lignans, flavonoids, vitamins, fiber, protein and other nutrients concentrated in one or more of the outer bran layers. Then separating the outer bran layer into three fractions, according to the desired bioactive components contained in the bran layers including antioxidants, complex phenolics, lignans, flavonoids, vitamins, fiber, protein and other nutrients. This allows the maximum benefit and value to be obtained from the bran fractions as dietary supplements, nutraceuticals, or as enriched food products.

In some preferred embodiments, a pet snack food, which is excellent in eating preference and low in risk of causing health impairment for pets due to excessive consumption of nutrition even if it is given in somewhat large amounts as a snack in addition to regular feeding, is provided. The pet snack food is a mixed composition including starch and animal protein as essential ingredients, and preferably further including optional ingredients such as minerals, vitamins, yeasts, and dietary fibers. It is preferable that the pet snack food is a bulky formed product having a foaming ratio of 3 to 20 formed by bulk-foaming extrusion using an extruder.

The invention discloses a medicinal auxiliary material production wastewater treatment technology, and relates to the treatment of wastewater in the medicinal auxiliary material preparation field. The technology comprises the following steps: wastewater is discharged to an equalizing tank I, and microcrystalline wastewater is discharged to an equalizing tank II; wastewater from the equalizing tank I is lifted to a coagulation precipitation tank I through a pump, and the obtained wastewater enters a Fenton catalytic oxidation tank for a reaction; and wastewater from the equalizing tank II and water discharged from the Fenton catalytic oxidation tank enter a coagulation precipitation tank II water discharged from the coagulation precipitation tank II and domestic sewage enter a homogenization tank and are uniformly mixed, the obtained water mixture enters a hydrolysis acidifying tank and then enters an intermediate water tank, wastewater from the intermediate water tank is lifted to an EGSB reaction tower, water discharged from the EGSB automatically flows to a bio-contact oxidation tank, water discharged from the bio-contact oxidation tank is discharged to a coagulation precipitation tank III, water discharged from the coagulation precipitation tank III is sent to a sand filter, and water from the sand filter is discharged to a clear water pool for standard reaching discharge. The medicinal auxiliary material production wastewater treatment technology has the advantages of simplicity, less energy consumption, low operation cost, stable and reliable treatment effect, strong impact load resistance ability, and realization of highest COD removal rate reaching above 90%.

The invention provides a preparation method of kiwi fruit powder. The preparation method includes the steps that after kiwi fruits are subjected to vacuum freeze drying in a vacuum freeze drying device, seed-pulp separation is carried out in a low-temperature seed-pulp separation device; vacuum freeze drying specifically includes the procedures that a temperature probe is placed in a sample disc containing the kiwi fruits, quick freezing is carried out on the kiwi fruits, the refrigeration rate is reduced to range from -20 DEG C to -30 DEG C from the room temperature of +25 DEG C, operation is completed within 2-3 hours, the kiwi fruit slices are frozen, the state is kept for 30 min to 2 hours, and then lyophilization is carried out; obtained pulp is smashed in a low-temperature pulp low-speed smasher, and the kiwi fruit powder is obtained. The method is simple in preparation process, and the content of vitamin C in the kiwi fruits can be reserved to the maximum degree.

The invention belongs to the field of fruit planting, and particularly relates to a grape planting method. The method includes the steps of cutting seedling culture, transplanting, topdressing, pest and disease control and daily management. According to the method, a series of scientific and reasonable planting steps are adopted, so that grape nutrition is guaranteed, and meanwhile the risk of pest and disease damage to grapes is effectively reduced; special fertilizer is sprayed to grapevines and soil, so that the fertility of soil is improved, and the growth of grapevines is guaranteed; organic fertilizer and inorganic fertilizer are sprayed to grapevines in a mixed mode, so that the growth of grapevine stem tips is promoted, the maturation stage of grapes comes earlier, the yield of grapes is effectively increased, the growth speed of grapes is higher, and the yield is higher.

The invention provides a tissue culture method for one-step seedling formation by using with-leaf petioles of Jingzhou pinellia ternate tissue-culture cluster seedlings. The method includes the following steps that 1, explants are selected, wherein Jingzhou pinellia ternate seeds are obtained, after disinfection treatment, germination is accelerated to form cluster buds, and the with-leaf petioles are selected as the explants for tissue culture; 2, the explants are inoculated into a seedling formation medium for culture until the seedlings grow to be 4-10 cm, wherein the seedling formation medium includes 0.5-1.2 mg / L of 1 / 2MS + IBA, 0.2-0.8 mg / L of NAA, 0.15-0.25 mg / L of TDZ, 3% of sucrose and 0.7% of agar. The with-leaf petioles of the Jingzhou pinellia ternate tissue-culture cluster seedlings serve as the explants, the special culture medium is adopted, the seedlings can be rapidly formed in only 45 days, the multiplication coefficient and seedling quality of the Jingzhou pinellia ternate seedlings are ensured (the rooting rate is 95% or more and the transplanting survival rate is 100%), and scale production is achieved.

The invention relates to a special feed additive for meat-purpose quail for resisting cold stress and improving meat quality, and the special feed additive is used for the general problems of frequent enteritis and poor meat quality of quail in northeast China caused by cold stress. Various ingredients carry out synergistic interaction to play the functions of resisting cold stress by procyanidolic ologomer and improving the meat quality, which are attempts and breakthroughs in the feed field. The additive is low in use amount, low in cost, environment-friendly, non-toxic and suitable for large-scale popularization and application.

The invention discloses an isolated streptomyces and biocontrol and growth promoting application thereof, and belongs to the field of biotechnology. The Streptomyces sp. DL70 of the invention is deposited in China Center for Type Culture Collection (CCTCC), the deposit number is CCTCC NO.M 2019475, and the deposit date is June20, 2019. The streptomyces is isolated from the deep rhizosphere soil ofhealthy plants along the coastal beach in Dalian, has low nutritional requirements and rapid cell growth, and can achieve an effective number of viable bacteria of not less than 1010 cfu / mL in 48 hours of liquid fermentation. According to the present invention, the Streptomyces DL70 and the fermentation products of the Streptomyces DL70 have highly effective antagonistic effects on a variety of plant pathogenic fungi, significantly promote the rapid growth of plants at the same time, and have obvious advantages of biocontrol and growth promoting application and industrial production potential.

The invention discloses a dwarf culture and compact planting cultivation method of idesia polycarpa. The problem that in the prior art, pest and disease damage prevention and treatment of the idesia polycarpa are difficult to perform, fruits are not easy to pick after the idesia polycarpa is fruited, the yield and quality of the fruits are not high, and the land utilization rate and the economic benefits are low. The method includes the following steps of 1, soil preparation and compact planting and 2, pruning and shaping; when pruned idesia polycarpa tree seedlings reach the full fruiting period, the height of each tree is 3-5 cm, the tree crowns are in a pagoda-like layered dispersion shape of 2.5*2.5 meters, three tree crowns are on the first layer, two tree crowns are on the second layer, 1-2 tree crowns are on the third layer, the gap between every two adjacent layers is 0.5-0.8 m, main branches on each layer are staggered up and down and are not overlapped and intersected, and the basic angle of each main branch is 50-60 degrees. The method is simple and convenient to operate, can effectively control the shapes of the idesia polycarpa trees and improve the land utilization rate, planting and picking are convenient, and the yield and quality of the idesia polycarpa fruits are improved.

The invention discloses albumen powder for tonifying spleen and losing weight, and a preparation method thereof. The albumen powder is prepared from the following raw materials in parts by weight: 1 to 2 parts of orange peel inner-layer white inner peel, 1 to 3 parts of white hyacinth bean, 1 to 3 parts of white kidney bean, 2 to 4 parts of exocarpium benincasae, 1 to 3 parts of poria cocos, 1 to 3 parts of rhizoma alismatis, 1 to 3 parts of grifola, 1 to 2 parts of rhizoma atractylodis macrocephalae, 0.5 to 1.5 parts of radix glycyrrhizae, 2 to 3 parts of semen cassiae, 0.3 to 0.7 part of folium sennae, 0.5 to 0.8 part of oat fiber, 0.3 to 0.5 part of apple fiber, 1 to 2 parts of folium nelumbinis powder, 1 to 3 parts of radix puerariae freeze-dried powder, and 3 to 5 parts of concentrated whey protein. The albumen powder for tonifying spleen and losing weight provided by the invention has the efficacy on effectively nourishing spleen and stomach, and invigorating spleen to remove dampness, is reasonable in nutritional supplement, ensures the nutritional requirement of a human body, meanwhile, is beneficial to reducing the acceptable daily intake of energy, achieves an effect on reducing blood glucose and losing weight, and is easy to assimilate, fine and smooth in taste, and mellow in flavor.

The invention relates to a fish feed, and especially relates to a low-cost plecoglossus altivelis compound feed. The invention also relates to a preparation method of the compound feed. The compound feed is composed of the following components in parts by weight: 10 to 20 parts of soybean meal, 10 to 20 parts of peanut meal, 10 to 20 parts of cotton meal, 8 to 16 parts of strong flour, 8 to 14 parts of rice bran, 6 to 10 parts of caloglossa leprieurii, 5 to 8 parts of dry clam worm powder, 5 to 8 parts of ulva powder, 4 to 8 parts of mung bean powder, 4 to 6 parts of pea powder, 4 to 6 parts of dry carrot powder, 4 to 6 parts of potato powder, 3 to 5 parts of cordate houttuynia, 3 to 5 parts of purslane, 2 to 4 parts of rhizoma kaempferiae, 1 to 3 parts of vallisneria natans, 1 to 3 parts of saxifrage, 2 to 4 parts of shell powder, 3 to 5 parts of fish oil, and 2 to 4 parts of xanthan gum. The cost of the compound feed is low, the raw materials are green and environment-friendly, the compound is attractive to plecoglossus altivelis, and the living rate is high.

The present invention relates to a feed additive for weaned piglets, a preparation method and an application thereof, and belongs to the field of feeds. The feed additive for the weaned piglets comprises the following raw materials of zinc oxide, oil, starch, a binder, and a disintegrant. In the feed additive for the weaned piglets, the zinc oxide is embedded with the auxiliary materials and an embedding rate is 70-80%. The additive can achieve the same anti-diarrhea effect as the prior art, at the same time greatly reduces use amount of the zinc oxide, achieves harm reduction and emission reduction, and can also solve a problem of feed palatability. The preparation method comprises the following steps: raw material mixing according to a ratio, then water spraying and granulating, and thendrying, shaping and sieving. The method is simple and rapid, good in condition controllability, and can enable the auxiliary materials to effectively embed the zinc oxide and control the embedding rate to a better range. The additive is added into a basic daily diet of the weaned piglets, can reduce diarrhea rate of the piglets, improves hair color of the piglets, at the same time is free of other zinc sources, and can meet nutritional needs of the piglets.

The invention discloses a cultivation method of camphor trees. Seeds are treated in combining with seed collecting, physical and chemical methods, seed quality of the camphor trees is remarkably improved, rate of emergence is greatly increased, and growing seedlings are of good quality; base fertilizer is applied in sowing, the base fertilizer is reasonable in formula, nutrients required by the camphor trees in growth can be provided, growing of the camphor tree seedlings are stimulated and regulated, the base fertilizer can serve as a nutrient modifier for soil to promote roots of the camphortrees to develop, and the metabolic function is enhanced; at the beginning of buds of the camphor trees, leaf fertilizer is sprayed, the leaf fertilizer is reasonable in formula and fast in effect and can be directly absorbed by plants, nutritional requirements of the camphor trees in growing are met, occurrence of diseases and pests can be reduced, disease resistance and resilience of the camphor trees are enhanced, survival rate is effectively increased, and the cultivation method is suitable for large-scale cultivation.

The invention provides a full-balance granulated feed for adult stichopus japonicus. The feed comprises a protein component, a starch component, a grease component, compound vitamins, compound mineral substances, a bacteria component, an alga component and Chinese herbal medicine components. The full-balance granulated feed for the adult stichopus japonicus disclosed by the invention has comprehensive and balanced nutrition, and is obtained by combining the characteristics of stichopus japonicus cultivated in a pond and the characteristics of feed raw materials on the basis of deeply researching nutrition physiological and ecological habits of the adult stichopus japonicus. After the feed disclosed by the invention is used for production, the survival rate and the weight increasing rate of the stichopus japonicus are obviously higher than those of stichopus japonicus fed by other feeds of similar brands; meanwhile, by virtue of the good cost performance of the feed disclosed by the invention, the cultivation cost of fishermen is reduced, and the increment of the fishermen is accelerated.

The invention discloses a compound fertilizer for controlling soil pollution. The compound fertilizer is used for improving soil for the alkalinity or acidity of soil to be repaired, after the pH of soil reaches an appropriate range, a chelating agent in the soil is used for heavy metal complexing, and a specific reactant generates antagonism with heavy metals in the soil, so that the complexing effect of the chelating agent for heavy meals is improved, and then the heavy metals in the soil are greatly fixed, the content of heavy metal ions in the soil is reduced, and the influence on plantingplants in the soil is reduced to a great extent. Moreover, edible fungus waste materials are added into the compound fertilizer, on one hand, the edible fungus waste materials react with heavy metalsto generate insoluble heavy metal complexes through organic matters, on the other hand, the edible fungus waste materials are also rich in abundant nutrient substances, and then the compound fertilizer also can meet the nutritional requirement of plants in soil under the condition of without adding other extra nutritional ingredients, so that the soil characteristics are indirectly improved.

A low-cost special mixed feed for Pelteobagrus fulvidraco is prepared from, by weight, 30-35 parts of earthworm powder, 20-25 parts of silkworm chrysalis powder, 10-15 parts of housefly larva powder, 10-15 parts of river snail powder, 5-10 parts of chicken blood, 5-10 parts of mulberries, 3-5 parts of Chinese toon leaves, 3-5 parts of Pentapanax leschenaultii,10-15 parts of corncob powder, 5-10 parts of fish meal, 2-3 parts of composite vitamins and 0.5-1 part of rare earth. The mixed feed is prepared through the following steps: mixing all above components, crushing the obtained mixture to 60-80 meshes, and carrying out puffing granulation. The mixed feed has the advantages of reasonable formula, convenient and easily available components, reduction of the use amount of the fish meal, reduction of the culture cost, effective improvement of the immunity of Pelteobagrus fulvidraco, and effective increase of the individual weight of adult Pelteobagrus fulvidraco.

The invention provides specific low-cost compound feed for pelteobagrus fulvidraco. The compound feed is prepared from the following components in parts by weight: 30-35 parts of earthworm powder, 20-25 parts of silkworm chrysalis meal, 10-15 parts of housefly larva powder, 10-15 parts of field snail powder, 5-10 parts of chicken blood, 5-10 parts of mulberry, 3-5 parts of toona sinensis leaves, 3-5 parts of gynostemma pentaphyllum, 10-15 parts of corncob powder, 5-10 parts of fish powder, 2-3 parts of vitamin complex and 0.5-1 part of rare-earth. The compound feed is prepared by mixing the components, grinding to 60-80 meshes, puffing and granulating. The specific low-cost compound feed is reasonable in formula, convenient and easily available in components, the breeding cost can be reduced, and the immunity and adult individual weight of pelteobagrus fulvidraco can be effectively improved.

The invention discloses a method for producing chitinase through fermentation of chaetomium globosum and application of the chitinase. According to the method, chaetomium globosum HY1 is adopted for strain activation, an activation culture medium comprises glucose, peptone, monopotassium phosphate and disodium hydrogen phosphate, then shake cultivation or fermentation tank cultivation are carriedout, a fermentation medium comprises soluble starch, glycerin, tryptone, yeast powder, corn steep liquor, NH4NO3, MnSO4, MgSO4, Na2HPO4, K2HPO4 and colloidal chitin, and chitinase produced through fermentation is used for preparing chitosan oligosaccharide. The culture method is simple, the nutritional requirement is not high, and the chitinase produced by liquid state fermentation is high in activity and has a relatively great industrial application value.

The invention discloses a preparation method and application of pseudomonas chlororaphis for resisting disease and promoting growth. A strain is pseudomonas chlororaphis HT66CCTCC No:M2013467. A main metabolite of the strain comprises phenazine-1-phenazine and phenazine-1 formamide. The strain is inoculated into a microorganism culture medium and is fermented to obtain a thallus with high concentration and the metabolite, and can be mixed with materials like powder active carbon, bentonite, swell soil, kaolin, diatomite, zeolite, calcium carbonate and the like to prepare a microbial agent; the quantity of viable organisms in a finished product is 1-8.5*10<9>cfu / mL. The tablet laboratory verifies that the pseudomonas chlororaphis has good control effect of pathogenic bacteria of rice sheath blight diseases, cotton fusarium wilt, gaeumannomyces graminis and watermelon fusarium wilt, and also has a good promotion function in the growth of a part of plants.

The invention relates to the field of fish feeds and particularly relates to a powdery compound feed for Anguilla bicolor in an elver phase. The powdery compound feed is prepared from the following components in percentage by weight: 49.0%-72.0% of fish meal, 17.0%-38.0% of pregelatinized starch, 1.0%-10.0% of fish oil, 0.5% of multi-mineral, 0.5%-2.0% of calcium dihydrogenphoshate, 0.2% of choline chloride, 0.5% of multi-vitamin and 0.1%-0.2% of coated vitamin C. Compared with a traditional feed, the powdery compound feed has comprehensive nutrition, high protein and low fat and can meet the requirement on nutrition of the Anguilla bicolor in an elver phase; the food coefficient is low and the feed conversion rate is high; the Anguilla bicolor in an elver phase, which is fed with the compound feed, has strong disease resistance and high digestion utilization rate; the production process of the compound feed is similar with that of a common powdery feed and thus the compound feed is convenient to popularize in a large scale.

The invention discloses a gene engineering bacterial strain used for producing phenazine-1-methanamide and its purpose; which is characterized in that knockout of psrA or rpeA gene in Pseudomonas chlororaphis HT66(Pseudomonas chlororaphis HT66) CCTCC NO: M 2013467 genome is carried out. According to the invention, a strain of Pseudomonas chlororaphis is performed with seed culture, fermentation, extraction, concentration and crystallization to obtain phenazine-1-methanamide with purity of more than 96%. After the deletion of psrA and rpeA gene in the bacterium genome, the fermentation output of phenazine-1-methanamide can reach as high as 1800ppm. The agar plate bacteriostasis tests found that phenazine-1-methanamide has obvious inhibition effect on common plant pathogenic fungi such as Rhizoctonia solani, fusarium oxysporum, pythium wltmum and Stevia Septoria, has good stability and activity, and has good application prospect.

The invention discloses whole wheat ration feed for pigs. All corn and partial soybean meal are replaced by wheat, dosage of the corn is reduced to 0 at present from original 60-70% and dosage of the soybean meal is reduced to 5-12% from original 20%; and sufficient wheat enzyme is added. When the wheat is more advantaged in price, feed cost is reduced while nutrition requirement for pig growth is fully guaranteed, and breeding incomes of farmers are increased. In a word, the feed disclosed by the invention is scientific in formula, balanced in nutrition and relatively low in cost, pigs fed with the feed are healthy, high in percentage of lean meat, good in meat quality, high in nutrition value and rapid in growth speed.

The invention discloses a method for fermenting and producing chitinase by Chaetomium globosa and its application. The strain is activated by using Chaetomium globosa HY1, and the activation medium includes glucose, peptone, potassium dihydrogen phosphate, dihydrogen phosphate Sodium; then shaker culture or fermenter culture, the fermentation medium includes soluble starch, glycerol, tryptone, yeast powder, corn steep liquor, NH 4 NO 3 , MnSO 4 , MgSO 4 、Na 2 HPO 4 、K 2 HPO 4 , Colloidal chitin, the chitinase produced by fermentation is used for the preparation of chitooligosaccharides. The cultivation method of the invention is simple, the nutritional requirements are not high, the chitinase produced by liquid fermentation has high activity, and has great industrial application value.

The invention relates to a special feed additive for meat-purpose quail for resisting cold stress and improving meat quality, and the special feed additive is used for the general problems of frequent enteritis and poor meat quality of quail in northeast China caused by cold stress. Various ingredients carry out synergistic interaction to play the functions of resisting cold stress by procyanidolic ologomer and improving the meat quality, which are attempts and breakthroughs in the feed field. The additive is low in use amount, low in cost, environment-friendly, non-toxic and suitable for large-scale popularization and application.

The invention relates to a method for separating and screening an oligographic bacterium producing protease. A sample separated by the method is acquired from lean oil of Gurbantunggut Desert of Xinjiang, a low-nutrient formula is adopted, and the separated and purified 36 strains of bacteria are oligotrophic. Primary screening is carried out by a gelatin liquefaction method, secondary screening is carried out by taking casein as a substrate, and a strain with high protease producing capacity is screened, wherein the thallus is rod-like and the colony is round. The method has high selectivityand efficiency; and the obtained strain has low nutrient requirement, high protease producing capacity and high temperature resistance, and can reduce cost when applied to enzyme preparation production. The oligographic bacterium probably becomes a protease producing bacterium with important application prospect.

The present invention discloses a carotenoid-producing Rhodotorula biobovata and its application, which has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, with the preservation number CGMCC No.20126 and the preservation date being June 22, 2020 . Rhodotorula biobversus ZQ38 provided by the present invention belongs to the source of marine microorganisms and is an excellent strain for producing natural carotenoids. The conditions are easy to control, the nutritional requirement is low and the yield of carotenoids is high, and the strain has broad application prospects in the biosynthesis of carotenoids.