A tissue culture process for mountain tung, essentially comprising the following steps: 1. sterilizing the culture medium under high temperature and pressure, 2. taking the auxiliary bud stem of good seed quality and plant quality as explant, sterilizing with alcohol and corrosive mercury chloride solution, washing with sterilized water, 3. pitching the sterilized explant into culture medium, and putting under artificial daylight lamp for differentiation and bud growth, 4. cutting bud and pitching it into enrichment culture medium, 5. cutting the tube bud and pitching it into sprout-strengthening culture medium, 6. removing the base callus and part of leaves of the tube strengthened sprout, leaving 3-5 leaves and pitching them into root-growing culture medium, 7. taking out the sprout with root for washing, replanting it to culture medium with chaff ash and brown soil, watering thoroughly, shading for 70% in the first 10 days, and then lighting gradually, 20 days later the sprout survivals with root growing out rate being above 85%, and totally lighting 35-45 days later.