21 results about "Thidiazuron" patented technology

This invention relates to a rapid induction culture medium and method for inducing bud formation from kiwifruit leaves to complete plants, belonging to the field of biotechnology. The callus induction culture medium for rapidly inducing bud formation from kiwifruit leaves includes MS medium, sucrose, 6-benzylaminopurine, naphthaleneacetic acid, thidiazuron, and agar, with the following weight ratios: MS medium 0.002%-0.006%, sucrose 0.01%-0.05%, 6-benzylaminopurine 0.000001%-0.000003%, naphthaleneacetic acid 0.0000002%-0.0000008%, thidiazuron 0.000001%-0.000003%, agar 0.006%-0.01%, and the balance being water. The formula for the callus induction bud culture medium is as follows: MS medium 0.002%-0.006%, sucrose 0.01%-0.05%, 6-benzylaminopurine 0.0000005%-0.000003%, naphthaleneacetic acid 0.00000005%-0.0000002%, agar 0.006%-0.01%, with the balance being water. This invention provides a rapid induction culture medium and method for kiwifruit leaves to complete plants, effectively inhibiting problems such as callus waterlogging, browning, and vitrification. It not only increases the number of buds but also improves the quality of buds, which is beneficial to the growth and differentiation of callus tissue, making it easier for it to differentiate into buds, thus laying a solid foundation for subsequent genetic transformation experiments.

The application provides a method for promoting the absorption of calcium elements by pineapple fruits, which comprises the following steps: three months after the pineapple plant is induced to bloom and four months after the pineapple plant is induced to bloom, gauze is soaked in an amino oxyacetic acid aqueous solution or a pyrazine amide aqueous solution, the soaked gauze is applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours; the gauze is secondly soaked in an indole-3-acetic acid aqueous solution, an indole-3-butyric acid aqueous solution, a phenylacetic acid aqueous solution or an alpha-naphthylacetic acid aqueous solution, and the soaked gauze is again applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours; the gauze is thirdly soaked in a 6-benzylaminopurine aqueous solution, a kinetin aqueous solution, a thidiazuron aqueous solution or a forchlorfenuron aqueous solution, and the soaked gauze is again applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours. The application can inhibit the formation of the pineapple fruit stem delimitation, promote the absorption of calcium by the fruits, reduce the incidence of pineapple water core disease from about 18% to about 0.6%, and significantly improve the income of fruit farmers.

The application provides a cotton defoliant containing a fluorine-containing thidiazuron derivative, and belongs to the technical field of plant growth regulators. The structural formula of the fluorine-containing thidiazuron derivative is as follows: wherein R is a benzene ring containing 0-5 fluorine atoms at any position. The defoliant can effectively reduce the use temperature of the defoliant, eliminate the phenomenon of withering but not falling after the defoliant is applied, and ensure the yield and quality of cotton.

The invention discloses a treating agent for promoting rapid dormancy breaking of cotton seeds as well as a preparation method and application of the treating agent, and belongs to the technical field of crop planting. The treating agent is prepared from the following components: 0.1 to 0.7 mg / L of indoleacetic acid, 0.5 to 2 mg / L of thidiazuron (TDZ), 0.1 to 0.5 percent (m / v) of humic acid modified bentonite, 0.8 to 2.6 percent (m / v) of humus soil and the balance of water. The method has the beneficial effects that the germination index of the treated cotton is increased by 48.89%-77.78% compared with 6.67% of a control group (ck), which indicates that the germination speed of the treated cotton seeds is obviously increased. The cotton germination can be effectively promoted, and possibility is provided for effectively resisting the adverse situation in the cotton seedling stage.

The invention belongs to the field of durian cultivation, and particularly relates to a durian flower forcing agent, a preparation method and application. According to the durian flower forcing agent, prohexadione calcium, potassium phosphite, benzyl benzyl aminopurine, thidiazuron and ethephon are reasonably combined, durian can be promoted to bloom in advance, the durian flower forcing agent is diluted and then sprayed to durian trees, budding of the durian trees can be achieved 9 days after spraying, and the budding time and the blooming time are shortened; meanwhile, the cluster number of durian flower buds can be remarkably increased, important guarantee is provided for increasing the yield of durian, and economic benefits of durian cultivation can be greatly increased.

The application discloses a method for creating luffa double haploid by distant hybridization, which comprises the following steps: applying brassinolide water solution to the pistil of luffa female flower which will bloom the next day; the next day, pollinating the luffa female flower to which the brassinolide water solution is applied by using the male flower of winter melon, and at the same time, applying thidiazuron water solution to the ovary and pistil of the luffa female flower; after the pollination, bagging and isolating the female flower; 3-6 hours and 20-26 hours after the pollination, applying thidiazuron solution to the ovary and the base of the fruit stalk; after harvesting the seed melon, taking the relatively full seeds to germinate and sow to obtain regenerated plants; training and transplanting the regenerated plants, and then normally cultivating and managing the plants. The thidiazuron and the brassinolide are combined to be used for distant hybridization induction, stimulate the embryo development, and improve the fruit setting rate; through the multiple thidiazuron treatment, the chromosome doubling is induced, the luffa double haploid is directly obtained, and the problem of low efficiency of the luffa double haploid is solved.

The invention discloses a salt-resistant water-saving coating agent for improving seedling emergence of wheat in saline-alkali soil of Hetao Plain as well as a preparation method and application thereof, and belongs to the technical field of agricultural planting. The salt-resistant water-saving coating agent is prepared from the following components: 2 to 5 percent (m / v) of citric acid modified bentonite, 0.3 to 1.8 percent (m / v) of bacillus metabolite, 0.4 to 0.7 percent (m / v) of calcium carboxymethyl cellulose, 3 to 8mg / L of thidiazuron and the balance of water. The seed coating agent has the beneficial effects that the 7-day germination rate of seeds treated by the seed coating agent in the Inner Mongolia original saline-alkali soil (3 per mill) is increased by 72% compared with that of a control group; meanwhile, the plant height is averagely increased by 5.3 cm compared with that of a CK group, and it is indicated that the wheat seeds are more suitable for germination and growth in the saline-alkali environment after being subjected to coating treatment. The method can effectively improve the adverse effect of the saline-alkali environment on wheat germination and growth, and a feasible technical scheme is provided for wheat planting in saline-alkali soil.

The invention relates to the technical field of plant culture, in particular to a culture medium and a culture method for culturing passiflora edulis tissue culture seedlings and a culture method of passiflora edulis seedlings. The induction culture medium is based on an MS culture medium and further comprises thidiazuron, 6-benayl aminopurine, indoleacetic acid, cane sugar and agar. The proliferation culture medium is based on an MS culture medium and further comprises the following components: 6-benayl aminopurine, indoleacetic acid, cane sugar and agar. The rooting culture medium is based on an MS culture medium and further comprises indolebutyric acid, indoleacetic acid, cane sugar and agar. The culture medium disclosed by the invention can be used for culturing passion flower tissue culture seedlings with a large rooting number and a high rooting rate, and a new way is provided for breeding of good passion flower varieties.

The invention discloses a treating agent for promoting wheat germination in Xinjiang saline-alkali soil and a preparation method and application thereof, and belongs to the technical field of agricultural planting. The treating agent is prepared from the following components: 0.2 to 1.6 mg / L of melatonin, 3 to 8 mg / L of thidiazuron (TDZ), 0.1 to 7 percent (m / v) of humic acid modified montmorillonite, 0.3 to 0.9 percent (m / v) of calcium carboxymethyl cellulose (CMC-Ca) and the balance of water. The wheat seed treatment agent has the beneficial effects that the germination rate of wheat seeds treated by the treatment agent in Xinjiang original saline-alkali soil is increased by 65% compared with that of a control group, so that the germination speed of the treated seeds in Xinjiang saline-alkali soil is obviously increased, the growth in the seedling stage is improved, and possibility is provided for effectively resisting the stress in the wheat seedling stage.

This invention discloses a composition and application method for shortening the seedling stage of grapes, relating to the field of grape cultivation technology. The composition comprises ten components, including 45-52 mg / kg of uniconazole and 1.8-2.2 mg / kg of thidiazuron. The plant-derived compound amino acids are a combination of soybean protein and corn germ enzymatically hydrolyzed small-molecule amino acids with a molecular weight <1000 Da. The preparation method involves pre-dissolving uniconazole and methyl jasmonate in 95% ethanol, then mixing them sequentially with the other components and adjusting the volume. Application is based on the timing of application, spraying 2-3 times at 30±5 day intervals when the lignification rate of the 3-5 nodes at the base of the new shoot reaches 60%-70% or the GA3 / ZT ratio is 3.0-4.0. Through the synergistic effect of its components, this invention can shorten the seedling stage of grapes to less than 3 years, achieving a fruiting rate of ≥80% in 2-year-old plants. It allows for precise control of excessive growth and promotes flowering, is safe and environmentally friendly, simple to operate, and low in cost, effectively solving the problem of long grape breeding cycles. It is suitable for the breeding of new grape varieties and the innovation of germplasm resources.

The invention relates to the technical field of corn dehydration, in particular to a composition and application thereof in promoting corn kernel dehydration. The composition comprises thidiazuron and ethephon. The inventor of the application finds for the first time that when the composition containing thidiazuron and ethephon is used for treating corn, the water content of corn kernels can be remarkably reduced while the corn yield is not reduced.

The invention discloses a treating agent for promoting germination of corn seeds in a saline-alkali environment as well as a preparation method and application of the treating agent, and belongs to the technical field of agricultural planting. The treating agent comprises the following components: 0.1%-4% (m / v) of hydrochloric acid modified bentonite, 3-8 mg / L of thidiazuron, 0.5-2% (m / v) of humus soil, 0.3%-0.8% (m / v) of calcium carboxymethyl cellulose and the balance of water. The germination rate of the corn seeds treated by the treatment agent provided by the invention in 150mM simulated saline-alkali liquid for 7 days is increased by 75% compared with that of a contrast; in an undisturbed soil environment in Xinjiang, the germination rate of the treated corn seeds is increased by 19% compared with that of a control group, which shows that the treated seeds are more suitable for germination and growth in a saline-alkali environment. The invention can effectively improve the adverse effect of saline-alkali environment on corn germination and growth, and provides a feasible technical scheme for corn planting in saline-alkali soil.

The present application belongs to the field of genetic engineering, and particularly relates to a cotton leaf-off precise regulation method based on CPD gene regulation. The present application finds that the brassinosteroid (BR) biosynthesis pathway is the key metabolic pathway for regulating the sensitivity of TDZ by analyzing the sensitivity difference of different cotton varieties to thidiazuron (TDZ) leaf-off agent, and the rate-limiting enzyme gene GhCPD is the core gene for determining the leaf-off sensitivity difference between varieties. The present application can early predict the leaf-off agent sensitivity of cotton varieties by detecting the expression level of GhCPD gene or the endogenous BR content; further, by regulating the expression level of GhCPD gene or applying exogenous 24-epibrassinolide (EBR), the leaf-off response of cotton to TDZ can be significantly enhanced.

The application provides a sunlight rose fruit bulking regulator, which comprises the following components in parts by weight: thidiazuron 0.2-0.3 parts, forchlorfenuron 0.28-0.42 parts, brassinolide 0.1-0.3 parts, non-ionic surfactant 1-5 parts, and alginic acid fertilizer 40-60 parts. The application utilizes thidiazuron and forchlorfenuron to strongly promote fruit cell division and increase cell quantity, utilizes brassinolide to comprehensively regulate plant physiological metabolism, and utilizes alginic acid fertilizer to provide rich nutrition for fruit development to achieve the effect of fruit bulking. The addition of the surfactant can gently penetrate the medicament solution to each part of the ear axis, fruit grains and the like, which can not only reduce the ear axis curling, but also reduce the loss of the medicament solution.

The invention provides a composition for improving summer black grape juice quality and application thereof, and belongs to the technical field of fruit tree planting. The composition capable of improving the quality of summer black grape juice without reducing the yield, provided by the invention, comprises a plant growth regulator 1 consisting of 35 to 45 mg / L of GA3 and 0 to 2 mg / L of chlorfenuron, or a plant growth regulator 1 consisting of 35 to 45 mg / L of GA3 and 1 mg / L of thidiazuron, and a plant growth regulator 2 consisting of 35 to 45 mg / L of GA3 and 0 to 4 mg / L of chlorfenuron. According to the composition provided by the invention, the quality indexes suitable for juice processing, such as fruit spike weight, soluble solid content, titratable acid content, aroma characteristic aroma substance content of fruits, flowers and fruits, pericarp anthocyanin content and the like, can be optimal, so that the quality indexes which are not beneficial to juice making are reduced, and the optimal comprehensive quality of the juice is finally ensured; the method is suitable for summer black grape production and application taking juice processing as a target.

The utility model discloses a continuous thidiazuron drying machine, and belongs to the technical field of thidiazuron drying equipment. Comprising a drying cylinder, an end cover and a support, and the end cover is arranged above the drying cylinder; the end cover is arranged above the drying cylinder in an up-down sliding mode, and the lower portion of the end cover is fixedly connected with a pre-drying box located in the drying cylinder. The pre-drying box is of a structure with the lower end open, and a rotating plate for blocking the lower end of the pre-drying box is rotationally connected into the drying cylinder. The crushing assembly capable of crushing caked thidiazuron is rotationally arranged in the pre-drying box; the scraping plate is located in the pre-drying box and attached to the inner wall of the pre-drying box. According to the device, the pre-drying box is arranged in the drying cylinder, so that caked thidiazuron can be subjected to crushing pretreatment, and the operation of transferring thidiazuron is reduced; and meanwhile, separation of the pre-drying box and the drying cylinder can be rapidly completed so that cleaning can be facilitated, and thidiazuron adhering to the inner wall of the pre-drying box can be scraped away through a scraping plate during separation.

The invention provides a towel gourd radiation induced embryoid seedling culture medium and a towel gourd double haploid creation method, and belongs to the technical field of breeding. The invention provides a loofah radiation mutagenesis embryoid seedling culture medium. The loofah radiation mutagenesis embryoid seedling culture medium comprises 4.74 g / L of an MS culture medium, 30 g / L of sucrose, 0.04 mg / L of thidiazuron, 0.225 mg / L of 6-benzylamino adenine and 7 g / L of agar. The radiation induced embryoid seedling culture medium for the towel gourd is used for culturing the radiation induced embryoid of the towel gourd, so that the radiation induced embryoid seedling of the towel gourd can be promoted, and the grown plant is identified as the double haploid of the towel gourd. Technical support is provided for creation of double haploids of towel gourds, high-quality parents are provided for cultivation of new varieties of towel gourds, and the creation process of the new varieties of towel gourds is accelerated.

The utility model discloses a dissolving kettle for producing a thidiazuron preparation, and relates to the technical field of dissolving kettles. The dissolving kettle for producing the thidiazuron preparation comprises a kettle body; the stirring and heating assembly comprises a rotating hole formed in the inner top wall of the kettle body and a rotating pipe rotationally arranged on the inner wall of the rotating hole, a rotating cylinder is rotationally arranged in the rotating pipe through two sealing bearings, and a plurality of stirring frames are fixedly arranged on the outer surface of the rotating cylinder at equal intervals; and electric heating plates are fixedly arranged on the inner walls of the plurality of stirring frames. The stirring and heating assembly is arranged, so that the aim of convective stirring can be achieved when the dissolving kettle is used for dissolving thidiazuron preparation raw materials in the actual use process, the mixing and dissolving efficiency of the thidiazuron preparation raw materials and a solution is effectively improved, and the mixing and dissolving efficiency of the thidiazuron preparation raw materials and the solution is improved. And meanwhile, the electric heating plate can uniformly and fully heat the solution in the stirring process, so that the heating efficiency is improved.

The invention discloses a culture medium for tissue culture and propagation of bamboo reed. The culture medium comprises an MS culture medium, 6-benzyladenine with the concentration of 0.5-1.0 mg / L, thidiazuron with the concentration of 0.1-0.5 mg / L, indolebutyric acid with the concentration of 0.08-0.1 mg / L and naphthylacetic acid with the concentration of 0.08-0.1 mg / L. The invention also discloses a tissue culture and rapid propagation method for bamboo reed, which comprises the following steps: collecting and disinfecting explants, germinating axillary buds, and performing propagation culture: performing first propagation by adopting a propagation culture medium and then performing second propagation, the second time of expanding propagation is directly transferred into a second culture medium or firstly cultured in an MS culture medium and then transferred into the second culture medium, and seedling strengthening, rooting and seedling hardening are carried out on the obtained aseptic seedlings in a cluster form: the seedlings are uncovered and hardened, and then the seedlings are transplanted into a deep hole tray to be cultivated by using a cultivation nutrient medium; and seedling formation: transplanting the cultivated seedlings into a greenhouse for culturing to obtain seedlings. According to the method, the culture and propagation speed of the bamboo reed can be increased, and a larger propagation multiple can be obtained within a short time.

The invention provides a plant growth regulator composition for promoting coloring and sugar increasing of grapes and application, belongs to the technical field of plant growth regulators, and can solve the technical problems that in the prior art, a large amount of chemical fertilizer is applied to obtain high-yield grapes, so that the soil texture is seriously deteriorated, and the fruit quality is reduced. The plant growth regulator composition for promoting color change and sugar increase of grapes comprises effective components of thidiazuron and 5-aminolevulinic acid, and the weight ratio of thidiazuron to 5-aminolevulinic acid is (1: 30)-(30: 1). The invention can significantly promote flower and fruit retention of grape trees, effectively regulate fruit color change and sugar increase, and achieve the purposes of flower retention, fruit promotion, stable yield, yield increase, quality improvement, crop stress resistance enhancement and the like.

The invention provides a method for promoting calcium absorption of pineapple fruits, which comprises the following steps of: soaking gauze in an aminooxyacetic acid aqueous solution or a pyrazinamide aqueous solution three months after flower forcing of a pineapple plant and four months after flower forcing, and applying the soaked gauze at the joint of the pineapple fruits and fruit stems for 2-5 hours; the gauze is soaked in the indole-3-acetic acid aqueous solution or the indole-3-butyric acid aqueous solution or the phenylacetic acid aqueous solution or the alpha-naphthylacetic acid aqueous solution for the second time, and the soaked gauze is applied to the joint of the pineapple fruit and the fruit stem for 2-5 hours again; the gauze is soaked in a 6-benayl aminopurine aqueous solution or a kinetin aqueous solution or a thidiazuron aqueous solution or a forchlorfenuron aqueous solution for the third time, and the soaked gauze is applied to the joint of the pineapple fruit and the fruit stem for 2-5 hours again. The pineapple stem separation layer can be inhibited from being formed, the calcium absorption of fruits is promoted, the incidence rate of the pineapple hydroheart disease is reduced from about 18% to about 0.6%, and the income of fruit farmers is remarkably increased.