89 results about "Methyl jasmonate" patented technology

The invention provides an analysis method for high-throughput evaluation of tobacco nicotine synthesis capability, and belongs to the technical field of nicotine synthesis capability analys.The analysis method comprises the steps that tobacco seeds are disinfected, germinated and cultured for 14 days to obtain seedlings, the seedlings are transferred to a control medium and an induction medium added with 3 [mu] M of methyl jasmonate to be cultured for 15 days, and the seedlings are obtained; the method comprises the following steps: removing water from an overground part for 15 minutes at 105 DEG C, drying at 65 DEG C until the weight is constant, grinding the overground part into powder, adding a NaOH solution and ethyl acetate containing an internal standard, carrying out vortex mixing uniformly, carrying out ultrasonication for 15 minutes, taking supernate, filtering to obtain sample extract liquor, inputting peak area data into an intelligent prediction model based on a hierarchical weight mechanism, and processing to obtain prediction correction data, the nicotine concentration of a sample is calculated by combining gas chromatography detection with a standard curve, the concentration is corrected by utilizing predicted correction data, finally, nicotine content data is calculated according to the corrected concentration and the dry weight of the sample, and high-precision evaluation of the tobacco nicotine synthesis capability is realized.

The invention provides a bumblebee attractant and a method for pollinating camellia oleifera by utilizing bumblebees, and belongs to the technical field of camellia oleifera pollination. The bumblebee attractant comprises the following components: eugenol, methyl jasmonate, glucose, a solubilizer and a diluent. The method for pollinating the camellia oleifera by using the bumblebees comprises the following steps: S1, 1-2 weeks before pollination, performing disinfection and insecticidal treatment on a camellia oleifera tree entering row; s2, 2-3 days before flowering of camellia oleifera, bumblebee beehives are placed in a camellia oleifera garden, and hydromel is fed; s3, when male flowers and female flowers of camellia oleifera bloom and the weather is sunny, spraying the bumblebee attractant to stigmas of the female flowers every day, and meanwhile, releasing pollinated bumblebees from the beehive until the flowering phase of the female flowers ends. Eugenol and methyl jasmonate in the attractant can synergistically improve the attraction effect on bumblebees, and the pollination rate of camellia oleifera is increased.

The invention provides a dendrobium devonianum epiphytic tea tree cultivation method, which belongs to an epiphytic plant planting technology, and is characterized in that a dendrobium devonianum root system can be closely fused with a tea tree trunk through treatment of'minimal trauma + callus inducer ', and a structural foundation is laid for material exchange. The jasmonic acid methyl ester stress with a specific concentration is applied to the dendrobium nobile in the key phenological period, the dendrobium nobile is accurately induced to synthesize and secrete beneficial secondary metabolites, the theanine content in the tea can be remarkably increased, the proportion of ester type catechin can be reduced, and therefore the fresh degree of the tea is remarkably increased, and the astringent taste of the tea is remarkably reduced. The stress resistance of the dendrobium devonianum and the tea trees treated by the method is also enhanced, and the disease occurrence rate is reduced. While high-quality dendrobium nobile is harvested, the quality and economic benefits of the produced tea leaves are synchronously improved.

The invention discloses a preparation method and application of a gynostemma pentaphylla exosome. According to the method, a high-activity callus is obtained by optimizing a callus induction culture medium, and a suspension culture system is established to realize biomass accumulation with the dry cell weight of 19.8 + / -0.7 g / L. A sequential progressive composite induction strategy (methyl jasmonate is added after chitosan is pre-induced for 24 hours) is innovatively adopted, so that the saponin content is remarkably increased to 102.6 + / -4.8 mg / g DW. Exosomes (P-Exos) are extracted from induced cells through an enzymolysis-magnetic bead affinity purification technology (WGA modified magnetic bead affinity capture after mild enzymolysis of a cracking buffer solution), the yield reaches 1.45 + / -0.08 mg / g cells, and the extraction efficiency is remarkably improved compared with that of a traditional gradient centrifugation method (C-Exos). Moreover, the gynostemma pentaphyllum exosome prepared by the invention can effectively improve the synthesis of skin collagen, has a better effect, can be applied to medicines for promoting skin wound repair or can be added into anti-aging cosmetics as a functional component, and has a very high application value.

The invention discloses a management method for improving cold resistance and drought resistance of macadamia nuts and increasing flowering quantity, which combines a four-stage dynamic management mode of soil improvement, cold resistance induction, drought resistance and tip control and flowering phase quality improvement, applies an organic fertilizer, a water-retaining agent and a composite slow-release fertilizer, induces root development, slowly releases the fertility of a chemical fertilizer, prolongs the action time of the chemical fertilizer, and improves the yield of the macadamia nuts. Brassinolide and potassium fulvate are compounded to activate cold-resistant gene expression of macadamia nut plants and improve cell membrane stability, so that cold resistance of the macadamia nut plants is improved, melatonin and methyl jasmonate perform double shoot control, an ABA signal channel of the plants is triggered, drought resistance of the plants is improved, flower bud differentiation is promoted, and growth of the macadamia nut plants is promoted. The synergistic effect of alginate oligosaccharide and gamma-aminobutyric acid enhances the elongation ability of a pollen tube, chemical agent stimulation is replaced by biological agent stimulation, the utilization rate of chemical agents is reduced, then the negative influence of the chemical agents on soil is reduced, and finally the fruit setting rate and the sustainable utilization of the soil are improved.

The invention provides a method for regulating and controlling aroma of grape fruits through an exogenous plant growth regulator, which comprises the following steps of: spraying three exogenous plant hormones, namely methyl jasmonate, phenylalanine and strigolactones, on 'Hanxiangmi' grape fruits with strong rose aroma, and analyzing the aroma composition and content of the treated grape fruits. The result shows that the rose fragrance intensity of the'Hanxiangmi 'grapes is enhanced after the jasmonic acid methyl ester treatment; after strigolactones treatment, components such as alpha-terpilenol, beta-ionone, nonanoic acid, geraniol and the like in grape fruits are prominent, so that the treated grapes are converted into mixed fragrance type grapes with flower fragrance, fruit fragrance and grass fragrance, and the fragrance layers are rich; and the treatment effect of phenylalanine is not obvious. The invention provides a reference for the research of strigolactone in fruit quality.

The invention provides a regulation and control method for prolonging the refreshing time of prunes, which comprises the following steps: disinfecting a phase-temperature fresh-keeping storehouse for 24 hours by using absolute ethyl alcohol, and ventilating for 8-10 hours to ensure a sterile environment. During harvesting, prunes which are free of plant diseases and insect pests, free of mechanical damage, complete in fruit cream and larger than or equal to 20% in sugar content are selected, and the temperature of the prunes is rapidly reduced to 2-2.2 DEG C within 10 h through portable differential pressure precooling equipment. After precooling, a methyl jasmonate aqueous solution is adopted for electrostatic atomization spraying, and 1-methylcyclopropene is used for airtight fumigation for 18-20 h to inhibit ethylene generation and respiration. And then, storing in a phase temperature warehouse at the temperature of-1.5 to-1.2 DEG C, controlling the relative humidity to be 85-87%, and carrying out intermittent nano atomization sterilization by using a hydrogen peroxide aqueous solution once every 15-18 days. According to the method, the fresh-keeping effect of the prunes is comprehensively improved, the safety and the quality of the fruits are ensured, and the fresh-keeping period is remarkably prolonged.

The invention discloses a method for adjusting the pore opening degree of Shanghai green, and belongs to the technical field of plant detection. Shanghai leaves are placed in 20mL of open liquid containing (1) 0.5% potassium chloride and 100 [mu] M / L calcium chloride or (2) 0.5% potassium chloride and 50 [mu] M / L brassinolide or (3) 0.5% potassium chloride and 10 [mu] M / L cytokinin or (4) 0.5% potassium chloride and 5 [mu] M / L gibberellin or (5) 0.5% potassium chloride and 100 [mu] M / L methyl jasmonate, culture is performed under illumination, and the illumination time is set to be 5h, so that the pore opening degree of Shanghai is remarkably increased. Compared with a control group (containing 0.5% of potassium chloride), the pore openness of the treatment is respectively increased by 33.5%, 43.5%, 43.4%, 27.1% and 70.2%. The method disclosed by the invention lays a technical foundation for rapidly screening out the exogenous environmental stimulation factor (environical stimuli) capable of increasing the opening degree of the pore.

The invention relates to the technical field of plant genetic engineering, in particular to a peanut bacterial wilt resistance related gene Ah6Q1KS5, an encoding protein thereof, a biological material and application of the gene Ah6Q1KS5 in disease-resistant breeding. Through whole genome identification and functional analysis, the gene Ah6Q1KS5 which is remarkably related to bacterial wilt resistance in a peanut SAMS gene family is screened, and the gene is induced by ralstonia solanacearum, salicylic acid (SA), abscisic acid (ABA) and methyl jasmonate (MeJA) to be remarkably expressed in a peanut bacterial wilt resistant variety H108. Peanut and tobacco plants over-expressing Ah6Q1KS5 can significantly reduce cell death, inhibit growth of ralstonia solanacearum, and enhance resistance to bacterial wilt. The molecular marker can be used for analyzing the molecular mechanism of peanut bacterial wilt resistance, provides new gene resources and technical means for cultivating bacterial wilt resistant peanut varieties, and has important application value in the field of peanut breeding.

The invention relates to the technical field of agricultural pest control, and particularly discloses a preparation method of an apolygus lucorum food attractant based on a composite plant extract. A preparation method of the apolygus lucorum food attractant based on the composite plant extract comprises the following steps: S1, treating a sesame raw material: taking the sesame raw material and crushing; s2, sesame extraction: taking the sesame particles obtained in S1, adding an ethanol water solution, performing ultrasonic-assisted extraction, and filtering to obtain a filtrate; s3, sesame concentration: performing vacuum concentration on the filtrate obtained in S2 to obtain a sesame extract; s4, material preparation: weighing corn germ powder, soybean powder, rice bran powder, a plant attractant methyl jasmonate, a saccharide aid maltitol and an antibacterial agent potassium sorbate, and uniformly mixing; s5, extracting; s6, mixing; and S7, drying and forming. The preparation method provided by the invention can be used for producing the special food attractant with slow release characteristic and high biological activity for the apolygus lucorum, and has the advantages that volatile active ingredients are efficiently dissolved out, and the structure is complete and reserved.

The invention discloses a preparation method of a prevention and control agent for winter low-temperature freezing injury of walnuts. The prevention and control agent is prepared from the following active ingredients in percentage by mass: 0.2 part of agricultural chitosan oligosaccharide, 1 part of carboxymethyl cellulose, 7.8 parts of jasmonic acid methyl ester, 0.3 part of monopotassium phosphate, 0.1 part of compound amino acid, 0.05 part of brassinolide and 100 parts of water. The method effectively solves the problems that the annual branches of the Xinjiang walnuts in winter are prone to freezing, and the existing prevention and control means are poor in effect, reduces the conditions of branch water loss and drying, tree body weakness and even death, can obviously improve the cold resistance of the annual branches and mixed buds, greatly relieves the freezing injury symptoms after adult trees and young trees are treated, and obviously optimizes physiological indexes.

The application discloses application of an exogenous hormone methyl jasmonate in improving quality of peony seed oil and a method thereof, a hormone response element in a key gene promoter of oil synthesis is analyzed, an exogenous hormone with regulation potential is screened, and the optimal exogenous hormone is MeJA; when spraying is performed twice at a spraying concentration of 100 μmol / L, the content of peony seed oil is significantly improved, and alpha-linolenic acid is also significantly improved, so that dual improvement of peony seed oil yield and quality is finally realized.

This invention discloses a method for culturing cherry cells and cherry cell exosomes. The culturing method includes the following steps: S1. Obtaining a cherry cell line; S2. Preparing a culture medium by adding salicylic acid and methyl jasmonate; S3. Culturing the cherry cell line obtained in step S1 under the following conditions: culturing at 20–30°C with UV-B illumination during the culture process. This invention improves the cherry cell culture method, increases production efficiency, and enhances the content of active ingredients, especially flavonoids and polyphenols, in the cherry cell culture. The cherry cell culture obtained by this invention, especially the exosomes extracted from it, has broad application potential in the cosmetics field.

The invention provides a method for inducing butterfly orchid to bloom, which mainly researches the secondary blooming characteristic of butterfly orchid, and promotes the blooming of butterfly orchid and shortens the secondary blooming time of butterfly orchid in combination with the use of a plant growth regulator and an environment management method. According to the technical scheme, the method is mainly used for evenly spraying an exogenous growth regulator on leaf surfaces, the exogenous growth regulator is a solution of gibberellin or cytokinin, methyl jasmonate and indole-3-acetic acid, and meanwhile environment management is matched, so that the purpose of shortening the flowering time of phalaenopsis amabilis is achieved.

The invention discloses a regulator for improving grape fruit fragrance and a preparation method thereof, and belongs to the technical field of grape cultivation, each liter of regulator comprises 50 mg-120 mg of chitosan oligosaccharide, 20 mg-50 mg of sodium selenite, 10 mg-20 mg of jasmonic acid methyl ester, 100 mg-250 mg of green tea polyphenol, 10 mg-20 mg of trehalose, 1 mL-5 mL of thymol nanoemulsion, 10 mg-20 mg of beta-cyclodextrin and 0.8 g-1. 0 g of ethyl alcohol, and water is used as a solvent. The content of strawberry ketone, ester and norisoprene compounds in the fruits to which the regulator is applied is obviously increased, nerol and beta-ionone are detected, and the variety of terpene aroma substances is enriched; after the fruit is stored in a high-temperature and high-humidity environment for 3 days, the aroma component change of the fruit to which the regulator is applied is relatively insignificant, and the aroma of the fruit is more stable than that of a reference substance and a blank reference substance.

The invention provides a tobacco and sweet potato relay intercropping field planting method and application thereof, and belongs to the technical field of agricultural planting. The planting method provided by the invention comprises the following steps: after tobacco is harvested, intensively burning diseased roots, diseased plants and weeds in a field, deeply ploughing soil in winter, carrying out soil drying and sterilization, applying an organic fertilizer or a special fertilizer for sweet potatoes by adopting a strip application mode, carrying out tobacco and sweet potato intercropping by adopting a single-side opposite planting mode, applying a bacillus siamensis XZ92 bacterial liquid and a bacillus velezensis XZ93 bacterial liquid to rhizosphere of the intercropped tobacco, and carrying out intercropping on the tobacco and the sweet potatoes by adopting a single-side opposite planting mode. And pouring methyl jasmonate liquid into the tobaccos, and supplementing trace elements in stages to finish planting. The comprehensive prevention and control system for the tobacco bacterial wilt is constructed by optimizing the relay intercropping space layout of tobacco and sweet potatoes, cooperatively applying strains with specific functions and methyl jasmonate and designing a staged nutrient supply scheme.

The invention discloses a composite elicitor for promoting taxus chinensis embryogenic stem cells to produce paclitaxel and application, and belongs to the technical field of biology. The compound elicitor consists of methyl jasmonate, indolebutyric acid and salicylic acid, and the working concentrations of the methyl jasmonate, the indolebutyric acid and the salicylic acid in a culture medium are respectively as follows: 50-800 mu mol / L of methyl jasmonate, 12.5-50 mu mol / L of indolebutyric acid and 0.1-0.5 mg / L of salicylic acid. Preferably, the jasmonic acid methyl ester is 200 [mu] mol / L, the indolebutyric acid is 25 [mu] mol / L, the salicylic acid is 0.5 mg / L, and 0.1-0.5 mg / L arachidonic acid can be selectively added. The composite elicitor is added in the early stage of the exponential growth phase of the taxus chinensis embryogenic stem cells, and the taxus chinensis embryogenic stem cells are continuously cultured for 5 days, so that the yield of paclitaxel reaches 27.52 mg / L and is 7.86 times that of blank control, and meanwhile, the maximum biomass is obtained. According to the invention, the synergistic interaction of the three elicitors is realized for the first time, the production period is remarkably shortened, a set of efficient and stable paclitaxel production system is established, and an innovative solution is provided for large-scale production of paclitaxel.

The invention belongs to the technical field of crop cultivation, and particularly relates to a rice aroma-enhancing and yield-increasing agent and application thereof, and the rice aroma-enhancing and yield-increasing agent comprises a primary aroma-enhancing and yield-increasing agent and a secondary aroma-enhancing and yield-increasing agent; the primary aroma and yield increasing agent is jasmonic acid methyl ester with the concentration of 50 [mu] mol / L-100 [mu] mol / L; the formula of the secondary aroma-enhancing and yield-increasing agent is as follows: each liter of the secondary aroma-enhancing and yield-increasing agent contains 0.8 to 1.2 g of ornithine, 25 to 35 mmol of trehalose, 1.0 to 1.4 g of vitamin B1, 1.8 to 2.2 g of vitamin B5, 1 to 3 mg of nano zinc oxide, 4 to 6 mg of sodium selenite, 1 to 3 g of KH2PO4 and the balance of water. The rice aroma-enhancing and yield-increasing agent disclosed by the invention has excellent performance when being applied to three aromatic rice varieties, the 2-AP content is remarkably increased, the yield is kept stable and even slightly increased, and the contradiction between aroma enhancement and yield reduction is successfully solved. The whole set of scheme is synergistic in component, simple and convenient to operate, environment-friendly, good in stability and universality and suitable for large-area production, popularization and application.

The invention discloses oolong wheat tea and a preparation method thereof, and belongs to the technical field of wheat tea processing. According to the technical scheme, the oolong wheat tea is prepared by blending barley extract liquor and oolong tea extract liquor. The barley fragrance index of the barley extract is greater than 0.1 and less than 0.9; the wheat aroma index is calculated according to the following formula: wheat aroma index = (2-acetylpyrrole + 2-ethylpyrazine + dimethylpyrazine + methylpyrazine) / (5-methylfurfural + 2-acetylfuran + furfural); the tea aroma index of the tea leaves for preparing the oolong tea extract liquor is greater than 2.5; the tea aroma index is calculated according to the following formula: tea aroma index = A / B; the total flower fragrance A is linalool, geraniol, cis-jasmonone, jasmonlactone, nerolidol, methyl jasmonate and indole; the total odor amount B is equal to the sum of cis-2, 4-heptadienal, trans-2, 4-heptadienal and indole. The method is applied to processing of oolong wheat tea, and the problems that the oolong wheat tea is not harmonious in fragrance, poor in palatability and prone to precipitation are solved.

The application discloses a kind of effectively prevent and control Gang Mei branch blight of nuisance pesticide and prevention and control method and application, specifically including the following components: methyl jasmonate 100-150mg / L, table 24-rape sprout lactone 0.01-0.5mg / L, 6-benzyl adenine 20-50mg / L, triacontanol 0.5-1mg / L, chitosan 300-600mg / L, organic silicon 100-400mg / L, phenylalanine 5-50mg / L, mannitol 5-30mg / L, betaine 50-300mg / L, 2 hydrated calcium chloride 150mg / L, eugenol 250mg / L, isopropenyl-5-chlorobenzimidazolone 0.02-0.05mg / L, alkyl phenol formaldehyde resin polyvinyl ether 800-1000mg / L, ethanol 10000-30000mg / L.The application is compounded by plant growth regulator, inorganic matter, amino acid and other components, induces Gang Mei planting seedling, so that it systematically obtains resistance, enhances defense, inhibits Gang Mei branch blight, stabilizes cell membrane and improves the ability of anti-abiotic adversity, and also helps to improve the content of Gang Mei medicinal component secondary metabolite.The pesticide prepared by the application does not contain pesticide chemical component, is safe, non-polluting, simple in use method, and can effectively prevent and control Gang Mei branch blight, improve Gang Mei yield, quality and medicinal value.

The invention discloses an application of an attractant for poliplius perfoliatus. According to the invention, two compounds, namely methyl jasmonate and d-dihydrocarvone, which have remarkable attraction activity on male Lipoliplius popliacea adults are reversely screened out through methods such as a molecular docking technology, electrophysiology and insect behavioristics, wherein the two compounds are methyl jasmonate and d-dihydrocarvone. A field trapping effect evaluation result shows that the methyl jasmonate has an obvious trapping effect on Lipoliplius popliacea male adults. Therefore, the methyl jasmonate can be used as the attractant for the male imago of the politeal-bead scales, and the lure containing the attractant can be used for trapping the male imago of the politeal-bead scales, so that the dependence on chemical pesticides is reduced, and the green prevention and control requirements are met.

The application provides a method for constructing a regulation network of a potato JA pathway in response to Cd stress. The method relates to the technical field of data processing, and comprises the following steps: obtaining multi-omics time series data of potatoes under the conditions of cadmium stress and methyl jasmonate treatment; performing signal decomposition and feature purification on the multi-omics time series data by using a weak stationary signal decomposition method based on sequence patterns to obtain a plurality of intrinsic mode components; constructing a dynamic regulation network model to capture multi-scale change patterns of response characteristics in the time dimension and the concentration dimension, thereby extracting deep features; inputting the intrinsic mode components into the trained dynamic regulation network model to output an attention weight matrix, thereby forming a regulation network representing the dynamic regulation intensity of the jasmonic acid pathway; and solving the optimal exogenous methyl jasmonate concentration for promoting biomass and inhibiting cadmium accumulation by optimizing an objective function. The application provides a high-precision model tool for analyzing the regulation mechanism of plant hormone signals under heavy metal stress.

The invention discloses a method for enhancing pine wood nematode disease resistance of larch through jasmonic acid signal mediated flavonoid biosynthesis. The method comprises the following steps that 1, the larch is inoculated with pine wood nematode; 2, performing high-throughput omics sequencing sampling and data analysis; 3, treating larch seedlings with methyl jasmonate; 4, the inhibition effect of the kaempferol and the citrus aurantium on the pine wood nematodes is obtained. According to the method, larch is taken as a research object, a disease stress system is constructed by artificially inoculating pine wood nematodes, leaf / stem tissues at different time points after infection are selected, and sequential multiomics analysis is carried out by combining transcriptome sequencing and a wide targeting metabolome technology; the theoretical basis and method for improving the pine wood nematode disease resistance of larch by regulating and controlling the biosynthesis of kaempferol and citrus aurantium through a jasmonic acid signal way are defined.

The invention discloses a cultivation method for promoting the growth of rhizoma bletillae and the accumulation of active ingredients, which comprises the following steps: carrying out foliage spraying on planted annual rhizoma bletillae seedlings by adopting a mesoporous silica loaded jasmonic acid methyl ester solution as a growth promoting agent, and meanwhile, applying trichoderma asperellum microcapsules to the roots of the annual rhizoma bletillae seedlings, the purposes of promoting growth of rhizoma bletillae and accumulation of active ingredients are achieved; by adopting the method, the growth of the rhizoma bletillae can be remarkably promoted, the content of active ingredients such as total polysaccharides, total phenols and total flavonoids in the rhizoma bletillae is effectively increased, meanwhile, the content of antioxidant ingredients is greatly increased, the yield and quality are both improved, and the method has important application value in rhizoma bletillae quality improvement cultivation.

The application discloses a night work activating composition containing multiple plant extract, which comprises the following components in mass: 3-5 parts of extract A, 10-15 parts of extract B, 2-4 parts of extract C, 1.5-3 parts of curcumin nano-liposome, 2-5 parts of lactobacillus fermentation filtrate, 1-3 parts of lock water magnetite, 0.1-0.3 parts of xanthan gum / acrylate compound thickening agent and 0.5-1 part of preservative composition. The night work activating composition containing multiple plant extract of the application is compounded by extract A with methyl jasmonate as a target component, extract B with rhodioside and syringic acid as target components and curcumin nano-liposome, compared with the same kind of products on the market, the antioxidant and anti-glycation capacity of the night work activating composition can be targetedly strengthened, the transdermal efficiency is improved, and the DNA repair effect and anti-inflammatory effect are improved.

The invention discloses a culture method for increasing the flavonoid content of suspension cells of codonopsis pilosula. The method comprises the following steps: (1) obtaining aseptic seedlings: disinfecting codonopsis pilosula seeds, inoculating the disinfected codonopsis pilosula seeds into an MS culture medium, and culturing to obtain the aseptic seedlings; (2) callus induction and subculture: taking the roots of the aseptic seedlings as explants, and performing induction and subculture on an MS culture medium containing 2, 4-D, 6-BA and KT to obtain loose calluses; (3) suspension culture: inoculating the callus into an MS liquid culture medium containing NAA, 6-BA and KT for suspension culture to obtain high-biomass codonopsis pilosula suspension cells; and (4) adding an elicitor / precursor: adding methyl jasmonate (MeJA) or sodium acetate (NaAC) into the suspension culture system, and inducing flavone synthesis and accumulation. By optimizing a plant growth regulator composition in the culture medium, the biomass of the suspension cells of the codonopsis pilosula is remarkably improved (the highest biomass reaches 57.57 g / L); by adding MeJA or NaAC, the daily average yield of flavone is increased to 16.69 mg / L / d, and the daily average yield of flavone is increased to 18.20 mg / L / d. According to the method, the culture period is about 70 days, and a feasible way is provided for efficient and large-scale production of codonopsis pilosula flavone.

The invention belongs to the technical field of spice raw material synthesis, and particularly relates to a synthesis method of methyl cis-dihydrojasmonate, which comprises the following steps: dissolving 1-heptyne in toluene, discharging into a first reaction tank, adding a cobalt catalyst, and discharging 1-bromine-ethylene and carbon monoxide gas into the first reaction tank; adding triphenylphosphine into a first catalyst recovery tank, discharging catalyst precipitates, and carrying out sectional distillation to distill, cool and recover 1-heptyne; absolute methanol is discharged into the layering tank; dimethyl malonate is discharged into a second reaction tank, sodium methoxide is slowly added, an addition reaction is carried out at the temperature of-5 DEG C to 0 DEG C, and then heating is carried out for a decarboxylation reaction; 4-bromo-dihydrojasmonic acid methyl ester obtained by distillation is discharged into a third reaction tank; and discharging methyl jasmonate with alpha, beta-double bonds into a fourth reaction tank for reaction. By simplifying the steps, improving the recovery rate of the catalyst and realizing continuous production, the problems of high cost, low yield, low recovery rate of the catalyst and the like in preparation of the methyl cis-dihydrojasmonate in the prior art are solved.

The invention provides a polyvinyl alcohol composite degradable fruit preservative film and a preparation method thereof. The preservative film is prepared from the following components: 2 to 5 weight percent of polyvinyl alcohol matrix, 0.1 to 0.5 weight percent of tea polyphenol, 0.05 to 0.2 weight percent of jasmonic acid methyl ester, 0.3 to 0.6 weight percent of nano silicon dioxide, 1 to 3 weight percent of plasticizer and the balance of deionized water, the preparation method comprises the following steps: (1) dissolving the PVA matrix at 80-100 DEG C; (2) ultrasonically dispersing the plasticizer, silicon dioxide and tea polyphenol at 40-60 DEG C; (3) pre-emulsifying methyl jasmonate by Tween-80, and stirring at a high speed; (4) coating and defoaming; and (5) gradient drying. According to the film, the mechanical property is enhanced through a PVA-silicon dioxide composite structure, free radicals are removed through tea polyphenol, ethylene synthase is inhibited through jasmonic acid methyl ester, slow release synergy of double active ingredients is achieved, the degradation rate in soil within 30 days is larger than or equal to 70%, and the requirements for efficient preservation and environmental protection are met.

The invention provides a pre-picking treatment method for improving mango fruit quality, which comprises the following steps: 25-35 days before picking, spraying a mixed agent A containing monopotassium phosphate, 24-epibrassinolide, paclobutrazol and Tween-80 on leaf surfaces; 15-25 days before picking, a mixed agent B containing monopotassium phosphate, 24-epibrassinolide, potassium cinnamate and Tween-80 is sprayed to the fruits twice in total, the interval is 7-10 days, and a mixed agent C containing monopotassium phosphate, compound sodium nitrophenolate, gamma-aminobutyric acid, methyl jasmonate and Tween-80 is sprayed at the same period. The pre-harvest treatment method can effectively promote the mango peel to turn red and improve the fruit quality. The pre-harvest treatment method is convenient to apply, easy to operate and friendly to the ecological environment, and has good economic benefits and social benefits.

The invention belongs to the technical field of plant biology, and particularly relates to a garlic aerial bulb induction and seed garlic propagation method integrating detoxification and rapid propagation functions, which comprises the following steps: firstly, performing heat shock and chemical demethylation synergistic double detoxification on garlic cloves; inoculating the detoxified stem tips into an induction culture medium containing a specific plant growth regulator and a jasmonic acid methyl ester precursor to directionally form aerial bulbs; then, high-efficiency proliferation is realized through two-stage propagation culture under the regulation and control of light and temperature; finally, the salicylic acid derivative and the growth-promoting rhizobacteria are combined to strengthen the field resistance. According to the method, efficient detoxification, rapid propagation and lasting resistance reconstruction can be synchronously achieved, the propagation coefficient is 3 times or above that of a traditional method, and the two-season virus re-infection rate is lower than 15%.