123 results about "Methyl jasmonate" patented technology

The invention provides an analysis method for high-throughput evaluation of tobacco nicotine synthesis capability, and belongs to the technical field of nicotine synthesis capability analys.The analysis method comprises the steps that tobacco seeds are disinfected, germinated and cultured for 14 days to obtain seedlings, the seedlings are transferred to a control medium and an induction medium added with 3 [mu] M of methyl jasmonate to be cultured for 15 days, and the seedlings are obtained; the method comprises the following steps: removing water from an overground part for 15 minutes at 105 DEG C, drying at 65 DEG C until the weight is constant, grinding the overground part into powder, adding a NaOH solution and ethyl acetate containing an internal standard, carrying out vortex mixing uniformly, carrying out ultrasonication for 15 minutes, taking supernate, filtering to obtain sample extract liquor, inputting peak area data into an intelligent prediction model based on a hierarchical weight mechanism, and processing to obtain prediction correction data, the nicotine concentration of a sample is calculated by combining gas chromatography detection with a standard curve, the concentration is corrected by utilizing predicted correction data, finally, nicotine content data is calculated according to the corrected concentration and the dry weight of the sample, and high-precision evaluation of the tobacco nicotine synthesis capability is realized.

The invention belongs to the technical field of bioengineering, and particularly relates to application of plant hormones in inhibition of fungal growth and inhibition of mycotoxin synthesis. The invention provides an application of a plant hormone in inhibition of fungal growth and inhibition of synthesis of mycotoxin, the plant hormone is methyl jasmonate, the mycotoxin is deoxynivalenol, and the final concentration of the methyl jasmonate is 30-4000 [mu] M. The invention provides an inhibitor for fusarium graminearum growth and DON synthesis, and provides a botanical inhibitor for formulating a safe, pollution-free and green wheat scab prevention and control strategy.

The invention provides a bumblebee attractant and a method for pollinating camellia oleifera by utilizing bumblebees, and belongs to the technical field of camellia oleifera pollination. The bumblebee attractant comprises the following components: eugenol, methyl jasmonate, glucose, a solubilizer and a diluent. The method for pollinating the camellia oleifera by using the bumblebees comprises the following steps: S1, 1-2 weeks before pollination, performing disinfection and insecticidal treatment on a camellia oleifera tree entering row; s2, 2-3 days before flowering of camellia oleifera, bumblebee beehives are placed in a camellia oleifera garden, and hydromel is fed; s3, when male flowers and female flowers of camellia oleifera bloom and the weather is sunny, spraying the bumblebee attractant to stigmas of the female flowers every day, and meanwhile, releasing pollinated bumblebees from the beehive until the flowering phase of the female flowers ends. Eugenol and methyl jasmonate in the attractant can synergistically improve the attraction effect on bumblebees, and the pollination rate of camellia oleifera is increased.

The invention provides a dendrobium devonianum epiphytic tea tree cultivation method, which belongs to an epiphytic plant planting technology, and is characterized in that a dendrobium devonianum root system can be closely fused with a tea tree trunk through treatment of'minimal trauma + callus inducer ', and a structural foundation is laid for material exchange. The jasmonic acid methyl ester stress with a specific concentration is applied to the dendrobium nobile in the key phenological period, the dendrobium nobile is accurately induced to synthesize and secrete beneficial secondary metabolites, the theanine content in the tea can be remarkably increased, the proportion of ester type catechin can be reduced, and therefore the fresh degree of the tea is remarkably increased, and the astringent taste of the tea is remarkably reduced. The stress resistance of the dendrobium devonianum and the tea trees treated by the method is also enhanced, and the disease occurrence rate is reduced. While high-quality dendrobium nobile is harvested, the quality and economic benefits of the produced tea leaves are synchronously improved.

The invention provides a preparation method for improving EGCG content of tea tree fresh leaves, which specifically comprises the following steps: spraying a mixed solution of salicylic acid and methyl jasmonate on new shoot leaves of a current year of tea tree, and picking 48 hours after spraying, so as to obtain the tea tree fresh leaves with high EGCG content, increase the volatile substance content of the tea tree, and improve the EGCG content of the tea tree fresh leaves. The growth of the tea trees can be promoted.

The invention discloses a preparation method and application of a gynostemma pentaphylla exosome. According to the method, a high-activity callus is obtained by optimizing a callus induction culture medium, and a suspension culture system is established to realize biomass accumulation with the dry cell weight of 19.8 + / -0.7 g / L. A sequential progressive composite induction strategy (methyl jasmonate is added after chitosan is pre-induced for 24 hours) is innovatively adopted, so that the saponin content is remarkably increased to 102.6 + / -4.8 mg / g DW. Exosomes (P-Exos) are extracted from induced cells through an enzymolysis-magnetic bead affinity purification technology (WGA modified magnetic bead affinity capture after mild enzymolysis of a cracking buffer solution), the yield reaches 1.45 + / -0.08 mg / g cells, and the extraction efficiency is remarkably improved compared with that of a traditional gradient centrifugation method (C-Exos). Moreover, the gynostemma pentaphyllum exosome prepared by the invention can effectively improve the synthesis of skin collagen, has a better effect, can be applied to medicines for promoting skin wound repair or can be added into anti-aging cosmetics as a functional component, and has a very high application value.

The invention discloses a plant growth regulator used in a rice panicle differentiation stage under high temperature stress and a use method thereof, the plant growth regulator comprises jasmonic acid methyl ester, fertilizer is applied 7 days after rice transplanting (tillering stage) and the panicle differentiation stage respectively, and a shallow water layer is kept after transplanting; spraying the plant growth regulator used in the rice panicle differentiation stage under high temperature stress at the initial stage of rice panicle differentiation; other management is carried out according to conventional field high-yield cultivation management. According to the plant growth regulator, by exogenous spraying of MeJA, the rice yield can be increased by 60% or above, multiple physiological indexes can be improved at the same time, the stress resistance of rice is enhanced, the nutritional requirements of rice spike growth and development are met, damage of high temperature stress to rice is relieved, the rice spikelet differentiation number is increased, and therefore the grain number per spike is increased, and the yield is increased. The method is beneficial for regulating endogenous growth hormones such as cytokinin of rice, optimizing the growth and development process and relieving the influence of high-temperature stress on the morphogenesis of young ears.

The invention provides a regulation and control method for prolonging the refreshing time of prunes, which comprises the following steps: disinfecting a phase-temperature fresh-keeping storehouse for 24 hours by using absolute ethyl alcohol, and ventilating for 8-10 hours to ensure a sterile environment. During harvesting, prunes which are free of plant diseases and insect pests, free of mechanical damage, complete in fruit cream and larger than or equal to 20% in sugar content are selected, and the temperature of the prunes is rapidly reduced to 2-2.2 DEG C within 10 hours by means of portable differential pressure precooling equipment. After precooling, a methyl jasmonate aqueous solution is adopted for electrostatic atomization spraying, and 1-methylcyclopropene is used for airtight fumigation for 18-20 hours to inhibit ethylene generation and respiration. And then, storing in a phase temperature warehouse at the temperature of-1.5 to-1.2 DEG C, controlling the relative humidity to be 85-87%, and carrying out intermittent nano-atomization sterilization by using a hydrogen peroxide aqueous solution once every 15-18 days. According to the method, the fresh-keeping effect of the prunes is comprehensively improved, the safety and the quality of the fruits are ensured, and the fresh-keeping period is remarkably prolonged.

The invention provides a culture medium composition for saussurea involucrate tissue culture and application thereof, and belongs to the field of plant tissue culture. On the basis of the MS culture medium, the culture medium composition also comprises the following components: 9 to 11 g / L of fructose, 9 to 11 g / L of glucose, 9 to 11 g / L of sucrose, 5 to 7 g / L of agar, 0.5 to 1.5 mg / L of 2, 4-dichlorphenoxyacetic acid, 0.05 to 0.1 mg / L of naphthylacetic acid, 0.05 to 0.2 mg / L of N-phenyl-N '-1, 2, 3-thiadiazole-5-urea, 400 to 600 mg / L of lactoalbumin hydrolysate, 0.2 to 0.4 g / L of activated carbon and 10 to 25 mg / L of methyl jasmonate. By integrating hormone, functional additives (lactoalbumin hydrolysate and activated carbon) and a nano material (silver / copper doped nano zinc oxide), a multi-level synergistic effect is formed, so that the yield of flavonoid compounds is improved on the basis of improving the callus induction rate of saussurea involucrata.

The invention discloses a management method for improving cold resistance and drought resistance of macadamia nuts and increasing flowering quantity, which combines a four-stage dynamic management mode of soil improvement, cold resistance induction, drought resistance and tip control and flowering phase quality improvement, applies an organic fertilizer, a water-retaining agent and a composite slow-release fertilizer, induces root development, slowly releases the fertility of a chemical fertilizer, prolongs the action time of the chemical fertilizer, and improves the yield of the macadamia nuts. Brassinolide and potassium fulvate are compounded to activate cold-resistant gene expression of macadamia nut plants and improve cell membrane stability, so that cold resistance of the macadamia nut plants is improved, melatonin and methyl jasmonate perform double shoot control, an ABA signal channel of the plants is triggered, drought resistance of the plants is improved, flower bud differentiation is promoted, and growth of the macadamia nut plants is promoted. The synergistic effect of alginate oligosaccharide and gamma-aminobutyric acid enhances the elongation ability of a pollen tube, chemical agent stimulation is replaced by biological agent stimulation, the utilization rate of chemical agents is reduced, then the negative influence of the chemical agents on soil is reduced, and finally the fruit setting rate and the sustainable utilization of the soil are improved.

The invention provides a method for regulating and controlling aroma of grape fruits through an exogenous plant growth regulator, which comprises the following steps of: spraying three exogenous plant hormones, namely methyl jasmonate, phenylalanine and strigolactones, on 'Hanxiangmi' grape fruits with strong rose aroma, and analyzing the aroma composition and content of the treated grape fruits. The result shows that the rose fragrance intensity of the'Hanxiangmi 'grapes is enhanced after the jasmonic acid methyl ester treatment; after strigolactones treatment, components such as alpha-terpilenol, beta-ionone, nonanoic acid, geraniol and the like in grape fruits are prominent, so that the treated grapes are converted into mixed fragrance type grapes with flower fragrance, fruit fragrance and grass fragrance, and the fragrance layers are rich; and the treatment effect of phenylalanine is not obvious. The invention provides a reference for the research of strigolactone in fruit quality.

The invention provides a regulation and control method for prolonging the refreshing time of prunes, which comprises the following steps: disinfecting a phase-temperature fresh-keeping storehouse for 24 hours by using absolute ethyl alcohol, and ventilating for 8-10 hours to ensure a sterile environment. During harvesting, prunes which are free of plant diseases and insect pests, free of mechanical damage, complete in fruit cream and larger than or equal to 20% in sugar content are selected, and the temperature of the prunes is rapidly reduced to 2-2.2 DEG C within 10 h through portable differential pressure precooling equipment. After precooling, a methyl jasmonate aqueous solution is adopted for electrostatic atomization spraying, and 1-methylcyclopropene is used for airtight fumigation for 18-20 h to inhibit ethylene generation and respiration. And then, storing in a phase temperature warehouse at the temperature of-1.5 to-1.2 DEG C, controlling the relative humidity to be 85-87%, and carrying out intermittent nano atomization sterilization by using a hydrogen peroxide aqueous solution once every 15-18 days. According to the method, the fresh-keeping effect of the prunes is comprehensively improved, the safety and the quality of the fruits are ensured, and the fresh-keeping period is remarkably prolonged.

The invention discloses a method for relieving high-temperature heat damage to rice, and relates to the technical field of rice cultivation. The method for relieving the high-temperature heat damage to the rice comprises the steps that when the rice suffers from high-temperature heat damage stress in the heading and flowering period, a first conditioning agent is sprayed to rice plants, and a second conditioning agent is sprayed before the first conditioning agent is applied; the first conditioning agent contains methyl jasmonate with the concentration of 1-4 mmol / L, tannic acid with the concentration of 10-40 mg / L, calcium nitrate with the concentration of 6-20 mg / L and betaine with the concentration of 12-30 mg / L; the second conditioning agent is mainly formed by mixing propylene glycol and humic acid according to the weight ratio of 1: (10-20). The problems that the effect of relieving high-temperature heat damage by independently using methyl jasmonate is unstable and the efficiency is not high are solved.

The invention discloses a method for adjusting the pore opening degree of Shanghai green, and belongs to the technical field of plant detection. Shanghai leaves are placed in 20mL of open liquid containing (1) 0.5% potassium chloride and 100 [mu] M / L calcium chloride or (2) 0.5% potassium chloride and 50 [mu] M / L brassinolide or (3) 0.5% potassium chloride and 10 [mu] M / L cytokinin or (4) 0.5% potassium chloride and 5 [mu] M / L gibberellin or (5) 0.5% potassium chloride and 100 [mu] M / L methyl jasmonate, culture is performed under illumination, and the illumination time is set to be 5h, so that the pore opening degree of Shanghai is remarkably increased. Compared with a control group (containing 0.5% of potassium chloride), the pore openness of the treatment is respectively increased by 33.5%, 43.5%, 43.4%, 27.1% and 70.2%. The method disclosed by the invention lays a technical foundation for rapidly screening out the exogenous environmental stimulation factor (environical stimuli) capable of increasing the opening degree of the pore.

The invention relates to the technical field of plant genetic engineering, in particular to a peanut bacterial wilt resistance related gene Ah6Q1KS5, an encoding protein thereof, a biological material and application of the gene Ah6Q1KS5 in disease-resistant breeding. Through whole genome identification and functional analysis, the gene Ah6Q1KS5 which is remarkably related to bacterial wilt resistance in a peanut SAMS gene family is screened, and the gene is induced by ralstonia solanacearum, salicylic acid (SA), abscisic acid (ABA) and methyl jasmonate (MeJA) to be remarkably expressed in a peanut bacterial wilt resistant variety H108. Peanut and tobacco plants over-expressing Ah6Q1KS5 can significantly reduce cell death, inhibit growth of ralstonia solanacearum, and enhance resistance to bacterial wilt. The molecular marker can be used for analyzing the molecular mechanism of peanut bacterial wilt resistance, provides new gene resources and technical means for cultivating bacterial wilt resistant peanut varieties, and has important application value in the field of peanut breeding.

The present invention discloses a special nutrient solution for Chinese rose, its preparation method and application method, belonging to the technical field of garden planting. Calculated by weight parts, the special nutrient solution for Chinese rose includes 30-150 parts of angelica polysaccharide powder, 5-10 parts of diammonia enzyme, 5-10 parts of methyl dihydrojasmonate, 100-300 parts of organic carbon solution and 500-800 parts of water. The present invention promotes the accumulation of anthocyanins in Chinese rose through various ways, maintains the red flower color of Chinese rose, and achieves the technical effect of improving the ornamental value of Chinese rose.

The invention relates to the technical field of agricultural pest control, and particularly discloses a preparation method of an apolygus lucorum food attractant based on a composite plant extract. A preparation method of the apolygus lucorum food attractant based on the composite plant extract comprises the following steps: S1, treating a sesame raw material: taking the sesame raw material and crushing; s2, sesame extraction: taking the sesame particles obtained in S1, adding an ethanol water solution, performing ultrasonic-assisted extraction, and filtering to obtain a filtrate; s3, sesame concentration: performing vacuum concentration on the filtrate obtained in S2 to obtain a sesame extract; s4, material preparation: weighing corn germ powder, soybean powder, rice bran powder, a plant attractant methyl jasmonate, a saccharide aid maltitol and an antibacterial agent potassium sorbate, and uniformly mixing; s5, extracting; s6, mixing; and S7, drying and forming. The preparation method provided by the invention can be used for producing the special food attractant with slow release characteristic and high biological activity for the apolygus lucorum, and has the advantages that volatile active ingredients are efficiently dissolved out, and the structure is complete and reserved.

The invention discloses a preparation method of a prevention and control agent for winter low-temperature freezing injury of walnuts. The prevention and control agent is prepared from the following active ingredients in percentage by mass: 0.2 part of agricultural chitosan oligosaccharide, 1 part of carboxymethyl cellulose, 7.8 parts of jasmonic acid methyl ester, 0.3 part of monopotassium phosphate, 0.1 part of compound amino acid, 0.05 part of brassinolide and 100 parts of water. The method effectively solves the problems that the annual branches of the Xinjiang walnuts in winter are prone to freezing, and the existing prevention and control means are poor in effect, reduces the conditions of branch water loss and drying, tree body weakness and even death, can obviously improve the cold resistance of the annual branches and mixed buds, greatly relieves the freezing injury symptoms after adult trees and young trees are treated, and obviously optimizes physiological indexes.

The application discloses application of an exogenous hormone methyl jasmonate in improving quality of peony seed oil and a method thereof, a hormone response element in a key gene promoter of oil synthesis is analyzed, an exogenous hormone with regulation potential is screened, and the optimal exogenous hormone is MeJA; when spraying is performed twice at a spraying concentration of 100 μmol / L, the content of peony seed oil is significantly improved, and alpha-linolenic acid is also significantly improved, so that dual improvement of peony seed oil yield and quality is finally realized.

The present invention discloses a method for improving the quality of Millettia reticulata, belonging to the technical field of Millettia reticulata cultivation. It applies a growth regulator in combination with spectral lighting. The growth regulator is methyl jasmonate, with a concentration of 100 - 400 μmol / L. The LED light quality of the spectral lighting is red light and blue light, and the total photon flux density of red light and blue light is 200 μmol / m 2 / s, and the light quality ratio of red light to blue light is 2 - 5:1. The method of the present invention can effectively improve the growth of Millettia reticulata seedlings, significantly increase the contents of the active ingredients total flavonoids, catechins, epicatechins and gallocatechins, especially the improvement effect of catechins is the best. In an embodiment, compared with the blank group, the contents of total flavonoids, catechins, epicatechins and gallocatechins are significantly increased by 83.05%, 314.72%, 81.03% and 179.62% respectively, and the effect is remarkable.

This invention discloses a method for culturing cherry cells and cherry cell exosomes. The culturing method includes the following steps: S1. Obtaining a cherry cell line; S2. Preparing a culture medium by adding salicylic acid and methyl jasmonate; S3. Culturing the cherry cell line obtained in step S1 under the following conditions: culturing at 20–30°C with UV-B illumination during the culture process. This invention improves the cherry cell culture method, increases production efficiency, and enhances the content of active ingredients, especially flavonoids and polyphenols, in the cherry cell culture. The cherry cell culture obtained by this invention, especially the exosomes extracted from it, has broad application potential in the cosmetics field.

The invention provides a method for inducing butterfly orchid to bloom, which mainly researches the secondary blooming characteristic of butterfly orchid, and promotes the blooming of butterfly orchid and shortens the secondary blooming time of butterfly orchid in combination with the use of a plant growth regulator and an environment management method. According to the technical scheme, the method is mainly used for evenly spraying an exogenous growth regulator on leaf surfaces, the exogenous growth regulator is a solution of gibberellin or cytokinin, methyl jasmonate and indole-3-acetic acid, and meanwhile environment management is matched, so that the purpose of shortening the flowering time of phalaenopsis amabilis is achieved.

The invention discloses a regulator for improving grape fruit fragrance and a preparation method thereof, and belongs to the technical field of grape cultivation, each liter of regulator comprises 50 mg-120 mg of chitosan oligosaccharide, 20 mg-50 mg of sodium selenite, 10 mg-20 mg of jasmonic acid methyl ester, 100 mg-250 mg of green tea polyphenol, 10 mg-20 mg of trehalose, 1 mL-5 mL of thymol nanoemulsion, 10 mg-20 mg of beta-cyclodextrin and 0.8 g-1. 0 g of ethyl alcohol, and water is used as a solvent. The content of strawberry ketone, ester and norisoprene compounds in the fruits to which the regulator is applied is obviously increased, nerol and beta-ionone are detected, and the variety of terpene aroma substances is enriched; after the fruit is stored in a high-temperature and high-humidity environment for 3 days, the aroma component change of the fruit to which the regulator is applied is relatively insignificant, and the aroma of the fruit is more stable than that of a reference substance and a blank reference substance.

The present invention provides a method for interplanting colorful leaves under an agarwood forest, comprising the following steps: S1. forest land selection, S2. colorful leaf seedling selection, S3. forest land arrangement, S4. colorful leaf seedling planting, S5. forest land management, S6. pest control, S7. harvesting. Forest land management is fertilization management and irrigation management. By applying water and fertilizer containing tyrosine, methionine, and methyl jasmonate, the content of 2-acetyl-1-pyrroline in colorful leaves is increased. Pest control effectively prevents and controls powdery mildew, anthracnose, aphids, and mites by spraying a biological insecticide composed of rotenone, osthole, and neem alcohol. The present invention plants colorful leaves under an agarwood forest, and the organic matter and trace elements produced during the growth of colorful leaves provide nutrients for agarwood trees and promote their growth. The covering of colorful leaves reduces the evaporation of water, keeps the soil moist, and is conducive to the growth of agarwood trees. Planting the colorful leaves under the agarwood forest avoids direct sunlight on the colorful leaves, which is conducive to the healthy growth of the colorful leaves.

The invention relates to a method for prolonging the ornamental period of a new shoot of cinnamomum camphora and increasing the linalool content. The method comprises the following steps: after a forest for cinnamomum camphora oil is put into production, spraying 0.50-1.85 mmol.L <-1 > methyl jasmonate once after the overground part of a plant is harvested every year and before shoot extraction in spring; or spraying 0.50-1.85 mmol.L <-1 > methyl jasmonate twice before sprouting in spring in a cinnamomum camphora ornamental forest, a leisure forest or a healthy forest; the method provided by the invention can promote sprouting and leaf expansion, prolong the ornamental period of new shoots of cinnamomum camphora, and improve the relative content of linalool in mature leaves. The spraying amount is 50-100 mL for each plant each time. The growth rate of the yield of branches and leaves of the cinnamomum camphora plants is 13-23%, the relative content of linalool in the branches and leaves of the cinnamomum camphora is increased by 11-22.3%, the sprouting time is advanced by 5-10 days, and the coloring and viewing time of new shoots is prolonged by 5-10 days; not only can the yield of branches and leaves of cinnamomum camphora be increased, but also the coloring viewing period of new shoots is greatly prolonged, and benefits can be improved by attracting and renewing people to keep healthy and leisure.

The invention provides a tobacco and sweet potato relay intercropping field planting method and application thereof, and belongs to the technical field of agricultural planting. The planting method provided by the invention comprises the following steps: after tobacco is harvested, intensively burning diseased roots, diseased plants and weeds in a field, deeply ploughing soil in winter, carrying out soil drying and sterilization, applying an organic fertilizer or a special fertilizer for sweet potatoes by adopting a strip application mode, carrying out tobacco and sweet potato intercropping by adopting a single-side opposite planting mode, applying a bacillus siamensis XZ92 bacterial liquid and a bacillus velezensis XZ93 bacterial liquid to rhizosphere of the intercropped tobacco, and carrying out intercropping on the tobacco and the sweet potatoes by adopting a single-side opposite planting mode. And pouring methyl jasmonate liquid into the tobaccos, and supplementing trace elements in stages to finish planting. The comprehensive prevention and control system for the tobacco bacterial wilt is constructed by optimizing the relay intercropping space layout of tobacco and sweet potatoes, cooperatively applying strains with specific functions and methyl jasmonate and designing a staged nutrient supply scheme.

The invention discloses a composite elicitor for promoting taxus chinensis embryogenic stem cells to produce paclitaxel and application, and belongs to the technical field of biology. The compound elicitor consists of methyl jasmonate, indolebutyric acid and salicylic acid, and the working concentrations of the methyl jasmonate, the indolebutyric acid and the salicylic acid in a culture medium are respectively as follows: 50-800 mu mol / L of methyl jasmonate, 12.5-50 mu mol / L of indolebutyric acid and 0.1-0.5 mg / L of salicylic acid. Preferably, the jasmonic acid methyl ester is 200 [mu] mol / L, the indolebutyric acid is 25 [mu] mol / L, the salicylic acid is 0.5 mg / L, and 0.1-0.5 mg / L arachidonic acid can be selectively added. The composite elicitor is added in the early stage of the exponential growth phase of the taxus chinensis embryogenic stem cells, and the taxus chinensis embryogenic stem cells are continuously cultured for 5 days, so that the yield of paclitaxel reaches 27.52 mg / L and is 7.86 times that of blank control, and meanwhile, the maximum biomass is obtained. According to the invention, the synergistic interaction of the three elicitors is realized for the first time, the production period is remarkably shortened, a set of efficient and stable paclitaxel production system is established, and an innovative solution is provided for large-scale production of paclitaxel.

The invention discloses a streptomyces strain S1 for preventing and controlling root rot, the streptomyces strain S1 is classified and named as Streptomyces peuceius, the Streptomyces peuceius is preserved in China General Microbiological Culture Collection Center on April 26, 2024, and the preservation number of the strain is CGMCC NO.30458. The invention further discloses a preparation method of the Streptomyces peuceius strain S1 for preventing and controlling root rot. The invention also discloses the application of the streptomyces strain S1 or the combined use of the streptomyces strain S1 and resource substances in prevention and control of root rot, and the resource substances are selected from at least one of methyl jasmonate and brassinolide.

The invention belongs to the technical field of crop cultivation, and particularly relates to a rice aroma-enhancing and yield-increasing agent and application thereof, and the rice aroma-enhancing and yield-increasing agent comprises a primary aroma-enhancing and yield-increasing agent and a secondary aroma-enhancing and yield-increasing agent; the primary aroma and yield increasing agent is jasmonic acid methyl ester with the concentration of 50 [mu] mol / L-100 [mu] mol / L; the formula of the secondary aroma-enhancing and yield-increasing agent is as follows: each liter of the secondary aroma-enhancing and yield-increasing agent contains 0.8 to 1.2 g of ornithine, 25 to 35 mmol of trehalose, 1.0 to 1.4 g of vitamin B1, 1.8 to 2.2 g of vitamin B5, 1 to 3 mg of nano zinc oxide, 4 to 6 mg of sodium selenite, 1 to 3 g of KH2PO4 and the balance of water. The rice aroma-enhancing and yield-increasing agent disclosed by the invention has excellent performance when being applied to three aromatic rice varieties, the 2-AP content is remarkably increased, the yield is kept stable and even slightly increased, and the contradiction between aroma enhancement and yield reduction is successfully solved. The whole set of scheme is synergistic in component, simple and convenient to operate, environment-friendly, good in stability and universality and suitable for large-area production, popularization and application.

The invention discloses oolong wheat tea and a preparation method thereof, and belongs to the technical field of wheat tea processing. According to the technical scheme, the oolong wheat tea is prepared by blending barley extract liquor and oolong tea extract liquor. The barley fragrance index of the barley extract is greater than 0.1 and less than 0.9; the wheat aroma index is calculated according to the following formula: wheat aroma index = (2-acetylpyrrole + 2-ethylpyrazine + dimethylpyrazine + methylpyrazine) / (5-methylfurfural + 2-acetylfuran + furfural); the tea aroma index of the tea leaves for preparing the oolong tea extract liquor is greater than 2.5; the tea aroma index is calculated according to the following formula: tea aroma index = A / B; the total flower fragrance A is linalool, geraniol, cis-jasmonone, jasmonlactone, nerolidol, methyl jasmonate and indole; the total odor amount B is equal to the sum of cis-2, 4-heptadienal, trans-2, 4-heptadienal and indole. The method is applied to processing of oolong wheat tea, and the problems that the oolong wheat tea is not harmonious in fragrance, poor in palatability and prone to precipitation are solved.