63 results about "Methyl jasmonate" patented technology

The invention provides a dendrobium devonianum epiphytic tea tree cultivation method, which belongs to an epiphytic plant planting technology, and is characterized in that a dendrobium devonianum root system can be closely fused with a tea tree trunk through treatment of'minimal trauma + callus inducer ', and a structural foundation is laid for material exchange. The jasmonic acid methyl ester stress with a specific concentration is applied to the dendrobium nobile in the key phenological period, the dendrobium nobile is accurately induced to synthesize and secrete beneficial secondary metabolites, the theanine content in the tea can be remarkably increased, the proportion of ester type catechin can be reduced, and therefore the fresh degree of the tea is remarkably increased, and the astringent taste of the tea is remarkably reduced. The stress resistance of the dendrobium devonianum and the tea trees treated by the method is also enhanced, and the disease occurrence rate is reduced. While high-quality dendrobium nobile is harvested, the quality and economic benefits of the produced tea leaves are synchronously improved.

The invention discloses a method for adjusting the pore opening degree of Shanghai green, and belongs to the technical field of plant detection. Shanghai leaves are placed in 20mL of open liquid containing (1) 0.5% potassium chloride and 100 [mu] M / L calcium chloride or (2) 0.5% potassium chloride and 50 [mu] M / L brassinolide or (3) 0.5% potassium chloride and 10 [mu] M / L cytokinin or (4) 0.5% potassium chloride and 5 [mu] M / L gibberellin or (5) 0.5% potassium chloride and 100 [mu] M / L methyl jasmonate, culture is performed under illumination, and the illumination time is set to be 5h, so that the pore opening degree of Shanghai is remarkably increased. Compared with a control group (containing 0.5% of potassium chloride), the pore openness of the treatment is respectively increased by 33.5%, 43.5%, 43.4%, 27.1% and 70.2%. The method disclosed by the invention lays a technical foundation for rapidly screening out the exogenous environmental stimulation factor (environical stimuli) capable of increasing the opening degree of the pore.

The invention relates to the technical field of plant genetic engineering, in particular to a peanut bacterial wilt resistance related gene Ah6Q1KS5, an encoding protein thereof, a biological material and application of the gene Ah6Q1KS5 in disease-resistant breeding. Through whole genome identification and functional analysis, the gene Ah6Q1KS5 which is remarkably related to bacterial wilt resistance in a peanut SAMS gene family is screened, and the gene is induced by ralstonia solanacearum, salicylic acid (SA), abscisic acid (ABA) and methyl jasmonate (MeJA) to be remarkably expressed in a peanut bacterial wilt resistant variety H108. Peanut and tobacco plants over-expressing Ah6Q1KS5 can significantly reduce cell death, inhibit growth of ralstonia solanacearum, and enhance resistance to bacterial wilt. The molecular marker can be used for analyzing the molecular mechanism of peanut bacterial wilt resistance, provides new gene resources and technical means for cultivating bacterial wilt resistant peanut varieties, and has important application value in the field of peanut breeding.

The invention relates to the technical field of agricultural pest control, and particularly discloses a preparation method of an apolygus lucorum food attractant based on a composite plant extract. A preparation method of the apolygus lucorum food attractant based on the composite plant extract comprises the following steps: S1, treating a sesame raw material: taking the sesame raw material and crushing; s2, sesame extraction: taking the sesame particles obtained in S1, adding an ethanol water solution, performing ultrasonic-assisted extraction, and filtering to obtain a filtrate; s3, sesame concentration: performing vacuum concentration on the filtrate obtained in S2 to obtain a sesame extract; s4, material preparation: weighing corn germ powder, soybean powder, rice bran powder, a plant attractant methyl jasmonate, a saccharide aid maltitol and an antibacterial agent potassium sorbate, and uniformly mixing; s5, extracting; s6, mixing; and S7, drying and forming. The preparation method provided by the invention can be used for producing the special food attractant with slow release characteristic and high biological activity for the apolygus lucorum, and has the advantages that volatile active ingredients are efficiently dissolved out, and the structure is complete and reserved.

The invention discloses a preparation method of a prevention and control agent for winter low-temperature freezing injury of walnuts. The prevention and control agent is prepared from the following active ingredients in percentage by mass: 0.2 part of agricultural chitosan oligosaccharide, 1 part of carboxymethyl cellulose, 7.8 parts of jasmonic acid methyl ester, 0.3 part of monopotassium phosphate, 0.1 part of compound amino acid, 0.05 part of brassinolide and 100 parts of water. The method effectively solves the problems that the annual branches of the Xinjiang walnuts in winter are prone to freezing, and the existing prevention and control means are poor in effect, reduces the conditions of branch water loss and drying, tree body weakness and even death, can obviously improve the cold resistance of the annual branches and mixed buds, greatly relieves the freezing injury symptoms after adult trees and young trees are treated, and obviously optimizes physiological indexes.

The application discloses application of an exogenous hormone methyl jasmonate in improving quality of peony seed oil and a method thereof, a hormone response element in a key gene promoter of oil synthesis is analyzed, an exogenous hormone with regulation potential is screened, and the optimal exogenous hormone is MeJA; when spraying is performed twice at a spraying concentration of 100 μmol / L, the content of peony seed oil is significantly improved, and alpha-linolenic acid is also significantly improved, so that dual improvement of peony seed oil yield and quality is finally realized.

This invention discloses a method for culturing cherry cells and cherry cell exosomes. The culturing method includes the following steps: S1. Obtaining a cherry cell line; S2. Preparing a culture medium by adding salicylic acid and methyl jasmonate; S3. Culturing the cherry cell line obtained in step S1 under the following conditions: culturing at 20–30°C with UV-B illumination during the culture process. This invention improves the cherry cell culture method, increases production efficiency, and enhances the content of active ingredients, especially flavonoids and polyphenols, in the cherry cell culture. The cherry cell culture obtained by this invention, especially the exosomes extracted from it, has broad application potential in the cosmetics field.

The invention provides a method for inducing butterfly orchid to bloom, which mainly researches the secondary blooming characteristic of butterfly orchid, and promotes the blooming of butterfly orchid and shortens the secondary blooming time of butterfly orchid in combination with the use of a plant growth regulator and an environment management method. According to the technical scheme, the method is mainly used for evenly spraying an exogenous growth regulator on leaf surfaces, the exogenous growth regulator is a solution of gibberellin or cytokinin, methyl jasmonate and indole-3-acetic acid, and meanwhile environment management is matched, so that the purpose of shortening the flowering time of phalaenopsis amabilis is achieved.

The invention provides a tobacco and sweet potato relay intercropping field planting method and application thereof, and belongs to the technical field of agricultural planting. The planting method provided by the invention comprises the following steps: after tobacco is harvested, intensively burning diseased roots, diseased plants and weeds in a field, deeply ploughing soil in winter, carrying out soil drying and sterilization, applying an organic fertilizer or a special fertilizer for sweet potatoes by adopting a strip application mode, carrying out tobacco and sweet potato intercropping by adopting a single-side opposite planting mode, applying a bacillus siamensis XZ92 bacterial liquid and a bacillus velezensis XZ93 bacterial liquid to rhizosphere of the intercropped tobacco, and carrying out intercropping on the tobacco and the sweet potatoes by adopting a single-side opposite planting mode. And pouring methyl jasmonate liquid into the tobaccos, and supplementing trace elements in stages to finish planting. The comprehensive prevention and control system for the tobacco bacterial wilt is constructed by optimizing the relay intercropping space layout of tobacco and sweet potatoes, cooperatively applying strains with specific functions and methyl jasmonate and designing a staged nutrient supply scheme.

The invention discloses a composite elicitor for promoting taxus chinensis embryogenic stem cells to produce paclitaxel and application, and belongs to the technical field of biology. The compound elicitor consists of methyl jasmonate, indolebutyric acid and salicylic acid, and the working concentrations of the methyl jasmonate, the indolebutyric acid and the salicylic acid in a culture medium are respectively as follows: 50-800 mu mol / L of methyl jasmonate, 12.5-50 mu mol / L of indolebutyric acid and 0.1-0.5 mg / L of salicylic acid. Preferably, the jasmonic acid methyl ester is 200 [mu] mol / L, the indolebutyric acid is 25 [mu] mol / L, the salicylic acid is 0.5 mg / L, and 0.1-0.5 mg / L arachidonic acid can be selectively added. The composite elicitor is added in the early stage of the exponential growth phase of the taxus chinensis embryogenic stem cells, and the taxus chinensis embryogenic stem cells are continuously cultured for 5 days, so that the yield of paclitaxel reaches 27.52 mg / L and is 7.86 times that of blank control, and meanwhile, the maximum biomass is obtained. According to the invention, the synergistic interaction of the three elicitors is realized for the first time, the production period is remarkably shortened, a set of efficient and stable paclitaxel production system is established, and an innovative solution is provided for large-scale production of paclitaxel.

The invention belongs to the technical field of crop cultivation, and particularly relates to a rice aroma-enhancing and yield-increasing agent and application thereof, and the rice aroma-enhancing and yield-increasing agent comprises a primary aroma-enhancing and yield-increasing agent and a secondary aroma-enhancing and yield-increasing agent; the primary aroma and yield increasing agent is jasmonic acid methyl ester with the concentration of 50 [mu] mol / L-100 [mu] mol / L; the formula of the secondary aroma-enhancing and yield-increasing agent is as follows: each liter of the secondary aroma-enhancing and yield-increasing agent contains 0.8 to 1.2 g of ornithine, 25 to 35 mmol of trehalose, 1.0 to 1.4 g of vitamin B1, 1.8 to 2.2 g of vitamin B5, 1 to 3 mg of nano zinc oxide, 4 to 6 mg of sodium selenite, 1 to 3 g of KH2PO4 and the balance of water. The rice aroma-enhancing and yield-increasing agent disclosed by the invention has excellent performance when being applied to three aromatic rice varieties, the 2-AP content is remarkably increased, the yield is kept stable and even slightly increased, and the contradiction between aroma enhancement and yield reduction is successfully solved. The whole set of scheme is synergistic in component, simple and convenient to operate, environment-friendly, good in stability and universality and suitable for large-area production, popularization and application.

The application provides a method for constructing a regulation network of a potato JA pathway in response to Cd stress. The method relates to the technical field of data processing, and comprises the following steps: obtaining multi-omics time series data of potatoes under the conditions of cadmium stress and methyl jasmonate treatment; performing signal decomposition and feature purification on the multi-omics time series data by using a weak stationary signal decomposition method based on sequence patterns to obtain a plurality of intrinsic mode components; constructing a dynamic regulation network model to capture multi-scale change patterns of response characteristics in the time dimension and the concentration dimension, thereby extracting deep features; inputting the intrinsic mode components into the trained dynamic regulation network model to output an attention weight matrix, thereby forming a regulation network representing the dynamic regulation intensity of the jasmonic acid pathway; and solving the optimal exogenous methyl jasmonate concentration for promoting biomass and inhibiting cadmium accumulation by optimizing an objective function. The application provides a high-precision model tool for analyzing the regulation mechanism of plant hormone signals under heavy metal stress.

The invention discloses a method for enhancing pine wood nematode disease resistance of larch through jasmonic acid signal mediated flavonoid biosynthesis. The method comprises the following steps that 1, the larch is inoculated with pine wood nematode; 2, performing high-throughput omics sequencing sampling and data analysis; 3, treating larch seedlings with methyl jasmonate; 4, the inhibition effect of the kaempferol and the citrus aurantium on the pine wood nematodes is obtained. According to the method, larch is taken as a research object, a disease stress system is constructed by artificially inoculating pine wood nematodes, leaf / stem tissues at different time points after infection are selected, and sequential multiomics analysis is carried out by combining transcriptome sequencing and a wide targeting metabolome technology; the theoretical basis and method for improving the pine wood nematode disease resistance of larch by regulating and controlling the biosynthesis of kaempferol and citrus aurantium through a jasmonic acid signal way are defined.

The application discloses a night work activating composition containing multiple plant extract, which comprises the following components in mass: 3-5 parts of extract A, 10-15 parts of extract B, 2-4 parts of extract C, 1.5-3 parts of curcumin nano-liposome, 2-5 parts of lactobacillus fermentation filtrate, 1-3 parts of lock water magnetite, 0.1-0.3 parts of xanthan gum / acrylate compound thickening agent and 0.5-1 part of preservative composition. The night work activating composition containing multiple plant extract of the application is compounded by extract A with methyl jasmonate as a target component, extract B with rhodioside and syringic acid as target components and curcumin nano-liposome, compared with the same kind of products on the market, the antioxidant and anti-glycation capacity of the night work activating composition can be targetedly strengthened, the transdermal efficiency is improved, and the DNA repair effect and anti-inflammatory effect are improved.

The invention discloses a culture method for increasing the flavonoid content of suspension cells of codonopsis pilosula. The method comprises the following steps: (1) obtaining aseptic seedlings: disinfecting codonopsis pilosula seeds, inoculating the disinfected codonopsis pilosula seeds into an MS culture medium, and culturing to obtain the aseptic seedlings; (2) callus induction and subculture: taking the roots of the aseptic seedlings as explants, and performing induction and subculture on an MS culture medium containing 2, 4-D, 6-BA and KT to obtain loose calluses; (3) suspension culture: inoculating the callus into an MS liquid culture medium containing NAA, 6-BA and KT for suspension culture to obtain high-biomass codonopsis pilosula suspension cells; and (4) adding an elicitor / precursor: adding methyl jasmonate (MeJA) or sodium acetate (NaAC) into the suspension culture system, and inducing flavone synthesis and accumulation. By optimizing a plant growth regulator composition in the culture medium, the biomass of the suspension cells of the codonopsis pilosula is remarkably improved (the highest biomass reaches 57.57 g / L); by adding MeJA or NaAC, the daily average yield of flavone is increased to 16.69 mg / L / d, and the daily average yield of flavone is increased to 18.20 mg / L / d. According to the method, the culture period is about 70 days, and a feasible way is provided for efficient and large-scale production of codonopsis pilosula flavone.

The invention belongs to the technical field of spice raw material synthesis, and particularly relates to a synthesis method of methyl cis-dihydrojasmonate, which comprises the following steps: dissolving 1-heptyne in toluene, discharging into a first reaction tank, adding a cobalt catalyst, and discharging 1-bromine-ethylene and carbon monoxide gas into the first reaction tank; adding triphenylphosphine into a first catalyst recovery tank, discharging catalyst precipitates, and carrying out sectional distillation to distill, cool and recover 1-heptyne; absolute methanol is discharged into the layering tank; dimethyl malonate is discharged into a second reaction tank, sodium methoxide is slowly added, an addition reaction is carried out at the temperature of-5 DEG C to 0 DEG C, and then heating is carried out for a decarboxylation reaction; 4-bromo-dihydrojasmonic acid methyl ester obtained by distillation is discharged into a third reaction tank; and discharging methyl jasmonate with alpha, beta-double bonds into a fourth reaction tank for reaction. By simplifying the steps, improving the recovery rate of the catalyst and realizing continuous production, the problems of high cost, low yield, low recovery rate of the catalyst and the like in preparation of the methyl cis-dihydrojasmonate in the prior art are solved.

The invention belongs to the technical field of plant biology, and particularly relates to a garlic aerial bulb induction and seed garlic propagation method integrating detoxification and rapid propagation functions, which comprises the following steps: firstly, performing heat shock and chemical demethylation synergistic double detoxification on garlic cloves; inoculating the detoxified stem tips into an induction culture medium containing a specific plant growth regulator and a jasmonic acid methyl ester precursor to directionally form aerial bulbs; then, high-efficiency proliferation is realized through two-stage propagation culture under the regulation and control of light and temperature; finally, the salicylic acid derivative and the growth-promoting rhizobacteria are combined to strengthen the field resistance. According to the method, efficient detoxification, rapid propagation and lasting resistance reconstruction can be synchronously achieved, the propagation coefficient is 3 times or above that of a traditional method, and the two-season virus re-infection rate is lower than 15%.

The invention discloses a fruit tree grafting growth promoter, and relates to the technical field of fruit tree cultivation. The growth promoter is prepared from the following raw materials in parts by weight: 0.01 to 0.05 part of methyl jasmonate, 0.1 to 0.3 part of salicylic acid nano microcapsules, 2 to 5 parts of bacillus velezensis, 1 to 3 parts of trichoderma harzianum, 5 to 10 parts of mesoporous silica nanoparticles, 3 to 6 parts of zinc alginate compound, 5 to 8 parts of herba portulacae extract, 2 to 4 parts of ginkgo leaf nano extract, 1 to 2 parts of chitosan oligosaccharide, 2 to 4 parts of pullulan and 100 to 200 parts of plasma activated water. The growth promoter disclosed by the invention realizes innovation of an action mechanism, prolongs the action lasting period, breaks through the adaptability limitation of a grafting technology, successfully utilizes and optimizes the micro-ecology of a grafting interface, and is environment-friendly and high in safety.

The application discloses a large-scale cutting of ginkgo and a cutting method thereof, and the cutting method comprises the following steps: cutting and dwarfing pruning a 4-5 year old ginkgo mother plant, pruning the lateral branches of the cut mother plant for multiple times, spraying a plant growth regulator after each pruning to promote the development of the lateral branches of the mother plant, cutting the branches on the lateral branches of the mother plant as cuttings, and inserting the cuttings into a substrate after treating the cuttings with a plant growth regulator. The method adopts the cutting and multiple pruning to promote the sprouting of the plant and generate the juvenile branch, greatly increases the number of cuttings, sprays 6-benzyladenine or brassinolide to improve the quality and quantity of the cuttings, and further uses methyl jasmonate, naphthalene acetic acid and uniconazole to jointly treat the cuttings to significantly improve the rooting rate of the cuttings. The method can be used for cultivating the leaf ginkgo and the ginkgo mother plant with excellent characteristics, significantly shortens the formation time of the cutting callus and adventitious roots, is simple and easy to operate, is suitable for large-scale cutting, and has popularization value in the asexual propagation of the ginkgo clone.

The invention discloses a method for increasing the anthocyanin content of aizoon stonecrop herb cells through sectional treatment, and belongs to the technical field of plant cell engineering. According to the method, dopamine hydrochloride is applied to induction regulation and control of plant cell anthocyanin synthesis for the first time, and methyl jasmonate and dopamine hydrochloride are sequentially added in two stages according to metabolism time sequence characteristics of aizoon stonecrop herb cells in the logarithmic phase: in the first stage, 20-50 [mu] mol / L of methyl jasmonate is added on the first day of the logarithmic phase, and 20-50 [mu] mol / L of dopamine hydrochloride is added on the second day of the logarithmic phase; activating a phenylpropane metabolic pathway in a targeted manner to synthesize an anthocyanin precursor; in the second stage, 5-25 [mu] mol / L of dopamine hydrochloride is added 24-48 h after the pathway is activated, active oxygen is removed, and carbon source distribution is directionally optimized. According to the method, the problem of action time sequence mismatching of traditional one-time compounding is solved, the anthocyanin content is increased by 90% or above, the problem that only the content can be increased but the browning rate is too high when single methyl jasmonate serves as an inducer is solved, the cell browning rate is lower than 3%, and the method is suitable for industrial large-scale production and has remarkable economic value and application prospects.

This invention belongs to the field of fruit tree cultivation technology, specifically relating to the application of methyl jasmonate in improving nitrogen use efficiency in plants. Applying a certain concentration of methyl jasmonate can significantly improve the nitrogen use efficiency (NUE) of plants under limited nitrogen supply, specifically manifested in a significant increase in plant dry and fresh weight, total root length, total surface area, total volume, number of root tips, number of lateral roots, and root activity; an increase in plant photosynthetic capacity and nitrogen content, and a decrease in the carbon-to-nitrogen ratio. This indicates that an appropriate concentration of methyl jasmonate effectively improves the NUE of apple plants under limited nitrogen supply, promoting plant growth and laying the foundation for further research into the functional mechanism of methyl jasmonate in improving apple NUE. Furthermore, these findings may be applied in the future to guide production practices and improve the nutritional status of fruit trees.

This invention discloses a composition and application method for shortening the seedling stage of grapes, relating to the field of grape cultivation technology. The composition comprises ten components, including 45-52 mg / kg of uniconazole and 1.8-2.2 mg / kg of thidiazuron. The plant-derived compound amino acids are a combination of soybean protein and corn germ enzymatically hydrolyzed small-molecule amino acids with a molecular weight <1000 Da. The preparation method involves pre-dissolving uniconazole and methyl jasmonate in 95% ethanol, then mixing them sequentially with the other components and adjusting the volume. Application is based on the timing of application, spraying 2-3 times at 30±5 day intervals when the lignification rate of the 3-5 nodes at the base of the new shoot reaches 60%-70% or the GA3 / ZT ratio is 3.0-4.0. Through the synergistic effect of its components, this invention can shorten the seedling stage of grapes to less than 3 years, achieving a fruiting rate of ≥80% in 2-year-old plants. It allows for precise control of excessive growth and promotes flowering, is safe and environmentally friendly, simple to operate, and low in cost, effectively solving the problem of long grape breeding cycles. It is suitable for the breeding of new grape varieties and the innovation of germplasm resources.

This invention relates to a method for delaying postharvest spore release in edible fungi, belonging to the field of edible fungi preservation technology. The method includes: subjecting freshly harvested edible fungi to one of the following treatments: dehydration treatment, methyl jasmonate treatment, or blue light-emitting diode treatment; and then storing the treated fungi at a temperature of 19-26°C and a relative humidity of 85%-90%. This method can significantly inhibit the opening rate of postharvest edible fungi and reduce spore release intensity. It is simple to operate, low in cost, environmentally friendly, safe, and effective, and has broad application prospects.

The application discloses a kind of nanocomposites and preparation method and application thereof, belong to the field of plant manganese stress resistance.The nanocomposite includes carrier and load, the load is uniformly loaded on the surface and inside of carrier, carrier is 2-methyl imidazole zinc, and the load is methyl jasmonate, the particle size of the nanocomposite is 100-300nm, and the mass ratio of methyl jasmonate and 2-methyl imidazole zinc is 1:5-20.The preparation method includes first preparing ZIF-8 at room temperature, then ZIF-8 is placed in MeJA methanol solution and soaked to prepare MeJA@ZIF-8 nanocomposite.The application is applied in mulberry manganese stress resistance.The nanocomposite provided by the application can achieve uniform particle size distribution, and good effect of manganese stress relief.

This invention discloses the application and method of the bicolor long-beaked fungus Ob-BHS48 in enhancing plant induced systemic resistance, belonging to the field of biocontrol technology. Through adjacent inoculation co-culture, no direct antagonistic or competitive effect was observed between the Ob-BHS48 strain and the Polish endothelial fungus strain CFCC53558. However, after inoculation of *Picea rubra* with the Ob-BHS48 strain, compared with uninoculated plants, a second inoculation with the Ep-53558 strain enhanced the disease resistance of *Picea rubra*, significantly reducing the area of ​​lesions on branches and trunks, similar to the effect of pretreatment with methyl jasmonate in increasing plant induced resistance. The accumulation of disease-resistant metabolites and the expression of disease-resistant genes were also upregulated. This indicates that the Ob-BHS48 strain can enhance the induced resistance of *Picea rubra*, achieving plant disease prevention. This invention broadens the application scope of dominant long-beaked fungi in forest disease control, opening up new avenues for developing biocontrol strategies for plant diseases. It has great application potential in forest protection and green agriculture, and can promote the green and sustainable development of these industries.

The invention discloses a composition for improving plant stress resistance, a growth regulator and application of the composition and the growth regulator, and belongs to the technical field of plant growth regulation. The composition is prepared from N-acetylcysteine and gamma polyglutamic acid, the mass ratio of the N-acetylcysteine to the gamma polyglutamic acid is (6-10): 3. The growth regulator comprises a growth promoting agent, N-acetylcysteine and gamma polyglutamic acid; the growth promoting agent is methyl jasmonate, brassinolide and betaine. The composition disclosed by the invention can improve the high-temperature stress resistance of the summer corn in the big bell mouth period, obviously improve the number and quality of spike flower development, further improve the maturing rate, and finally achieve the effect of improving the spike number per unit area, the grain number per unit area and the grain yield; the growth regulator disclosed by the invention can further realize stress resistance and high yield, is used for related planting areas with frequent and repeated high-temperature heat damage in a large bell mouth period in summer corn planting areas, effectively reduces the high-temperature damage, improves the yielding ability and the yield stability of the summer corn, and has a relatively wide application value.

The application discloses a spraying agent for improving mechanical harvesting effect of flue-cured tobacco leaves and application thereof, and relates to the technical field of tobacco production, in particular to a spraying agent for improving mechanical harvesting effect of flue-cured tobacco leaves and application thereof. The spraying agent comprises the following components: 100-200 micromoles per liter of methyl jasmonate, 100-200 micromoles per liter of abscisic acid, 50-100 millimoles per liter of ethanol, and the rest is water. When used, the spraying agent is used in an amount of 50 mL per plant, and is uniformly sprayed on the front and back surfaces of the tobacco leaves 1-3 days after the tobacco plants are topped. The spraying agent has the combined effects of the functions of methyl jasmonate in significantly enhancing the stress resistance of tobacco leaves and the effects of abscisic acid in coordinating physiological and biochemical metabolism of the tobacco plants and promoting normal maturation of the tobacco leaves, so that the tobacco leaf resistance to maturity and the duration of suitable maturity are improved, the quality of the tobacco leaves is improved, the leaf breakage rate, the leaf missing rate, the leaf drop rate and the tobacco stalk damage rate in mechanical harvesting of the flue-cured tobacco leaves are significantly reduced, the mechanical harvesting effect of the flue-cured tobacco leaves is effectively improved, and the spraying agent is simple to prepare and high in safety.

This invention relates to the field of crop genetics and breeding technology, and particularly to a rapid breeding method for high-CBD industrial hemp. The invention provides a rapid breeding method for high-CBD industrial hemp, which involves constructing a high-segregation-potential F1 generation base population through high-purity parental selection and reciprocal crosses; using oleic acid combined with methyl jasmonic acid to construct a targeted metabolic stress screening system for active ingredient synthesis; completing only one in vitro targeted screening in the F1 generation to identify the core target genotype; then continuously backcrossing to aggregate the superior traits of the recurrent paternal parents, ultimately selecting a line with high CBD content; furthermore, subsequent backcross generations are completed using greenhouse short-day generation combined with rapid, non-destructive in vivo detection technology, eliminating the need for repeated tissue culture operations; finally, after self-pollination purification, a target line with stable traits is obtained.

The invention relates to the technical field of agricultural microbial preparations, and discloses a sweet potato root rot antagonistic fungus preparation as well as a preparation method and an application method thereof. The preparation consists of a trichoderma harzianum and trichoderma atroviride compound strain, a modified charcoal-humic acid organic carrier, a chitosan-sodium alginate microcapsule wall material, a dextrin-trehalose stabilizer and a sodium salicylate / methyl jasmonate synergistic factor, and is prepared by adopting a solid state fermentation process, a fluidized bed drying process, a microcapsule embedding process and a fluidized bed granulation process. The field control effect reaches 78%-92%, the lasting period is 3-4 months, and the pesticide composition has no inhibition effect on beneficial microorganisms in soil.

The invention discloses application of a cinnabar tobacco glandular hair specific gene in acylated demethylated nicotine synthesis and aroma improvement and a method, and belongs to the fields of biochemistry, molecular biology and agricultural application. The specific gene is NtNAT1-NtNAT4, the nucleotide sequences of the specific gene are respectively shown as SEQ ID NO.1-4, the gene is highly expressed in glandular hair, prokaryotic expression and enzymatic activity analysis verify that the gene has acyltransferase activity, and jasmonic acid methyl ester can efficiently induce the expression of the gene. The gene participates in tobacco nicotine metabolism, and the encoded protein of the gene can catalyze acylation of demethylated nicotine, increase the accumulation amount of acylated demethylated nicotine and improve the tobacco fragrance. The invention provides a method for improving the tobacco aroma, and the method has important application value in tobacco modification and breeding.