Method for improving the quality of Millettia reticulata

Abstract

The present invention discloses a method for improving the quality of Millettia reticulata, belonging to the technical field of Millettia reticulata cultivation. It applies a growth regulator in combination with spectral lighting. The growth regulator is methyl jasmonate, with a concentration of 100 - 400 μmol / L. The LED light quality of the spectral lighting is red light and blue light, and the total photon flux density of red light and blue light is 200 μmol / m 2 / s, and the light quality ratio of red light to blue light is 2 - 5:1. The method of the present invention can effectively improve the growth of Millettia reticulata seedlings, significantly increase the contents of the active ingredients total flavonoids, catechins, epicatechins and gallocatechins, especially the improvement effect of catechins is the best. In an embodiment, compared with the blank group, the contents of total flavonoids, catechins, epicatechins and gallocatechins are significantly increased by 83.05%, 314.72%, 81.03% and 179.62% respectively, and the effect is remarkable.

Description

Technical Field

[0001] The present invention relates to the technical field of plant cultivation, specifically to the technical field of Spatholobus suberectus cultivation. More specifically, the present invention relates to a method for promoting the synthesis and accumulation of total flavonoids, catechins, epicatechins, and gallocatechins, the active ingredients of Spatholobus suberectus, and improving the quality of Spatholobus suberectus by using growth regulators in combination with spectral lighting. Background Art

[0002] Spatholobus suberectus comes from the dried vine stems of the leguminous plant Spatholobus suberectus Dunn. It is recorded in ancient herbal classics as "the holy medicine for the blood meridian" and is the main raw material for Chinese patent medicines such as Jinji Capsules and Huahong Tablets. The main active ingredient of Spatholobus suberectus is flavonoid compounds, among which the content of catechins is the highest, and it has functions such as promoting hematopoiesis. At present, commercial Spatholobus suberectus medicinal materials basically come from wild resources. However, the wild resources of Spatholobus suberectus in China are on the verge of exhaustion, and the Spatholobus suberectus in the Chinese medicinal materials market is mainly imported from ASEAN countries such as Vietnam. To solve the prominent problem of the contradiction between supply and demand in the Spatholobus suberectus market caused by the reduction of wild resources, research on Spatholobus suberectus planting technology has been reported at present, but it is only limited to the investigation of survival rate and biomass, and less attention is paid to the quality of Spatholobus suberectus.

[0003] In recent years, LED spectral lighting has been widely used in the fields of plant factories, crop production, space agriculture, etc. Research shows that using appropriate LED spectral lighting can promote the growth and development of plants, improve photosynthetic efficiency, and regulate the accumulation of active ingredients. When plants perceive more red light through "light-harvesting antennas", they will release a hormone, convert light energy into more chemical energy, and promote plants to grow stronger. When the blue light receptors of plants perceive a higher level of blue light, they will trigger a hormone response to help the stems of plants grow thicker.

[0004] Literature 1 (Xu Daquan et al. Effects of light quality on plant growth and development. Plant Physiology Journal 2015, 51(8): 1217-1234) records the main research progress in aspects such as the biological effects, biochemical mechanisms, and practical applications of various lights such as red, blue, yellow, green, and infrared and ultraviolet radiation on plants, and points out that lights of different light qualities or wavelengths have significantly different biological effects, including different effects on the morphological structure and chemical composition of plants, photosynthesis, and the growth and development of organs.

[0005] Literature 2 (Chinese Patent, Publication No. CN108834742A, Publication Date: November 20, 2018) discloses a method for promoting the growth and development of Pleurotus eryngii by using an LED plant growth light source, but this method is suitable for the industrial production of Pleurotus eryngii to improve efficiency.

[0006] Document 3 (Chinese Patent, Publication No. CN 112042418 A, Publication Date: December 8, 2020) describes a method for improving plant yield and quality by adjusting the ratio of green and blue light, but the plants targeted are leafy vegetables.

[0007] It can be seen that there is currently no report on regulating the content of active ingredients in Millettia reticulata by using the spectrum illumination of light-emitting diodes (LEDs).

[0008] As an important plant growth regulator, methyl jasmonate is widely involved in regulating various physiological and biochemical reactions of plants. Document 4 (Feng Mengjie, et al. Research Progress on Jasmonins Regulating Plant Growth and Development. Plant Physiology Journal 2015, 51(4): 407-412) records that jasmonins can regulate plant growth and development (such as the development of reproductive organs, the growth of seedlings, etc.); participate in the synthesis of anthocyanins and other plant secondary metabolites; mediate the resistance responses of plants to biotic stresses such as insects and pathogens and abiotic stresses such as drought, low temperature, and ultraviolet rays. However, it is unknown how methyl jasmonate affects the synthesis and accumulation of active ingredients in Millettia reticulata, as well as the optimal usage and dosage. Therefore, how to use growth regulators in combination with the LED spectrum illumination method to promote the synthesis and accumulation of active ingredients in Millettia reticulata is a key problem that has not been solved by those skilled in the art. Summary of the Invention

[0009] An object of the present invention is to provide an effective and feasible method for improving the quality of Millettia reticulata by using a growth regulator in combination with spectrum illumination, which promotes the growth of Millettia reticulata and the synthesis and accumulation of the active ingredients total flavonoids, catechin, epicatechin, and gallocatechin in Millettia reticulata, thereby improving the quality of Millettia reticulata.

[0010] To achieve these objects and other advantages of the present invention, the method for improving the quality of Millettia reticulata provided by the present invention applies a growth regulator to the Millettia reticulata plants while cooperating with spectrum illumination. The LED light quality of the spectrum illumination is red light and blue light, and the total photon flux density of the red light and blue light is 100-400 μmol / m 2 / s, and the light quality ratio of the red light and blue light is 2-5:1.

[0011] Preferably, in the method for improving the quality of Millettia reticulata, the growth regulator is methyl jasmonate, and the concentration is 100-400 μmol / L.

[0012] Preferably, in the method for improving the quality of Millettia reticulata, the total photon flux density is 200 μmol / m 2 / s, the light quality ratio of the red light and blue light is 2:1 or 3:1 or 4:1 or 5:1, and the photoperiod is 16 h / d.

[0013] Preferably, in the method for improving the quality of Millettia reticulata, the light quality ratio of red light to blue light is 2:1.

[0014] Preferably, in the method for improving the quality of Millettia reticulata, the concentration of methyl jasmonate is 100 μmol / L or 200 μmol / L or 300 μmol / L or 400 μmol / L, and the spraying amount is 30 mL / plant.

[0015] Preferably, in the method for improving the quality of Millettia reticulata, the concentration of methyl jasmonate is 200 μmol / L.

[0016] Preferably, in the method for improving the quality of Millettia reticulata, the light quality ratio of red light to blue light is 2:1, the total photon flux density of red light and blue light is 200 μmol / m 2 / s, and the photoperiod is 16 h / d;

[0017] The methyl jasmonate is used to prepare a methyl jasmonate solution with absolute ethanol as the solvent, the solution concentration is 200 μmol / L; the spraying amount is 30 mL per plant, and it is sprayed at 9 am every day, with a time interval of 7 days between each spraying;

[0018] The cultivation temperature of Millettia reticulata is 25 °C, and the relative air humidity for cultivation is 70%.

[0019] Preferably, in the method for improving the quality of Millettia reticulata, the quality of Millettia reticulata refers to the contents of total flavonoids, catechins, epicatechins, and gallocatechins.

[0020] The present invention provides an application of a plant growth regulator methyl jasmonate in increasing the overall content or the content of any one substance of total flavonoids, catechins, epicatechins, and gallocatechins in Millettia reticulata, wherein the Millettia reticulata plants are treated according to any one of the above methods.

[0021] The present invention provides an application of a light source system in increasing the overall content or the content of any one substance of total flavonoids, catechins, epicatechins, and gallocatechins in Millettia reticulata, wherein the LED light quality of the light source system is red light and blue light, the total photon flux density of red light and blue light is 100 - 400 μmol / m 2 / s, the light quality ratio of red light to blue light is 2 - 5:1, and the Millettia reticulata plants are treated according to any one of the above methods.

[0022] The present invention has at least the following beneficial effects:

[0023] 1. The method of the present invention combines the treatment of Millettia reticulata with the LED spectrum illumination of the ratio of methyl jasmonate growth regulator and red and blue light quality, which can effectively improve the growth of Millettia reticulata seedlings and significantly increase the contents of total flavonoids, catechins, epicatechins and gallocatechins in the active ingredients. In particular, the improvement effect of catechins is the best.

[0024] 2. In one embodiment of the present invention, the light quality ratio of red light and blue light in the LED spectrum illumination is 2:1, the total photon flux density of red light and blue light is 200 μmol / m 2 / s, the light cycle is 16 h / d, the concentration of the methyl jasmonate solution of the growth regulator is 200 μmol / L, and the spraying amount is 30 mL per plant. As a result, it is found that the contents of total flavonoids, catechins, epicatechins and gallocatechins are significantly increased by 83.05%, 314.72%, 81.03% and 179.62% respectively, and the effect is significant.

[0025] Other advantages, objectives and features of the present invention will be partially reflected by the following description and partially understood by those skilled in the art through the research and practice of the present invention. Brief Description of the Drawings

[0026] Figure 1 It is a photo of the growth status of Example 1;

[0027] Figure 2 It is a comparison chart of the contents of total flavonoids, catechins, epicatechins and gallocatechins in the active ingredients of Millettia reticulata in Comparative Example 1, Example 1, Example 2, Example 3 and Example 4 under different light quality treatments on the 60th day;

[0028] Figure 3 It is a comparison chart of the contents of total flavonoids, catechins, epicatechins and gallocatechins in the active ingredients of Millettia reticulata in Comparative Example 1, Example 1, Example 5, Example 6 and Example 7 under different concentrations of growth regulator treatments on the 60th day;

[0029] Figure 4 It is a comparison chart of the contents of total flavonoids, catechins, epicatechins and gallocatechins in the active ingredients of Millettia reticulata in Comparative Example 1, Comparative Example 2, Comparative Example 5 and Example 5 under different growth regulation methods on the 60th day;

[0030] Figure 5 It is a comparison chart of the contents of total flavonoids, catechins, epicatechins and gallocatechins in the active ingredients of Millettia reticulata in Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 under different total photon flux densities on the 60th day;

[0031] Figure 6Comparison chart of the contents of total flavonoids, catechin, epicatechin, and gallocatechin, the active ingredients of Millettia reticulata in Comparative Example 1, Comparative Example 5, Comparative Example 6, and Comparative Example 7 under different growth regulators on the 60th day. Detailed implementation mode

[0032] The following further elaborates on the present invention in conjunction with the embodiments, so that those skilled in the art can implement it with reference to the text of the specification.

[0033] Example 1

[0034] This example provides a method for improving the quality of Millettia reticulata by combining a growth regulator with LED spectral lighting, including spraying a growth regulator on the leaf surface of Millettia reticulata while combining with LED spectral lighting. Select Millettia reticulata plants with similar and good growth as test materials and plant them in the plant factory of the Guangxi Zhuang Autonomous Region Medicinal Botanical Garden; the cultivation temperature of Millettia reticulata is 25 °C, and the relative humidity of the cultivation air is 70%.

[0035] The light quality ratio of red light to blue light in the LED spectral lighting is 2:1, and the total photon flux density of red light and blue light is 200 μmol / m 2 / s, and the light cycle is 16 h / d.

[0036] The growth regulator is a methyl jasmonate solution, and the methyl jasmonate solution is prepared with 1 mL of absolute ethanol as the solvent, and the concentration of the methyl jasmonate solution is 100 μmol / L.

[0037] The specific spraying amount of the growth regulator sprayed on the leaf surface of Millettia reticulata is 30 mL per plant, sprayed at 9 am every day, and the time interval between each spraying is 7 d.

[0038] 6 biological replicates are set for each treatment, and the morphological changes of Millettia reticulata are observed and samples are taken every 15 d. After 60 d of conventional management, the contents of the active ingredients of Millettia reticulata are measured uniformly.

[0039] Example 2

[0040] This example provides a method for improving the quality of Millettia reticulata by combining a growth regulator with LED spectral lighting, including spraying a growth regulator on the leaf surface of Millettia reticulata while combining with LED spectral lighting. Select Millettia reticulata plants with similar and good growth as test materials and plant them in the plant factory of the Guangxi Zhuang Autonomous Region Medicinal Botanical Garden; the cultivation temperature of Millettia reticulata is 25 °C, and the relative humidity of the cultivation air is 70%.

[0041] The LED spectral lighting method uses a light quality ratio of red light to blue light of 3:1, and the total photon flux density of red light and blue light is 200 μmol / m 2 / s, and the light cycle is 16 h / d.

[0042] The growth regulator is a methyl jasmonate solution. The methyl jasmonate solution is prepared with 1 mL of absolute ethanol as the solvent, and the solution concentration is 100 μmol / L.

[0043] The specific spraying amount of the growth regulator sprayed on the leaves of Millettia reticulata is 30 mL per plant in total. It is sprayed at 9 am every day, and the time interval between each spraying is 7 days.

[0044] Six biological replicates are set for each treatment. The morphological changes of Millettia reticulata are observed and samples are taken every 15 days. After 60 days of conventional management, the contents of active ingredients in Millettia reticulata are measured uniformly.

[0045] Example 3

[0046] This example provides a method for improving the quality of Millettia reticulata by combining a growth regulator with LED spectral lighting, including spraying the growth regulator on the leaves of Millettia reticulata and simultaneously combining with LED spectral lighting. Select Millettia reticulata plants with similar and good growth as test materials and plant them in the plant factory of the Medicinal Plant Garden of Guangxi Zhuang Autonomous Region; the cultivation temperature of Millettia reticulata is 25 °C, and the relative air humidity for cultivation is 70%.

[0047] The LED spectral lighting method uses a light quality ratio of red light to blue light of 4:1, the total photon flux density of red light and blue light is 200 μmol / m 2 / s, and the photoperiod is 16 h / d.

[0048] The growth regulator is a methyl jasmonate solution. The methyl jasmonate solution is prepared with 1 mL of absolute ethanol as the solvent, and the solution concentration is 100 μmol / L.

[0049] The specific spraying amount of the growth regulator sprayed on the leaves of Millettia reticulata is 30 mL per plant in total. It is sprayed at 9 am every day, and the time interval between each spraying is 7 days.

[0050] Six biological replicates are set for each treatment. The morphological changes of Millettia reticulata are observed and samples are taken every 15 days. After 60 days of conventional management, the contents of active ingredients in Millettia reticulata are measured uniformly.

[0051] Example 4

[0052] This example provides a method for improving the quality of Millettia reticulata by combining a growth regulator with LED spectral lighting, including spraying the growth regulator on the leaves of Millettia reticulata and simultaneously combining with LED spectral lighting. Select Millettia reticulata plants with similar and good growth as test materials and plant them in the plant factory of the Medicinal Plant Garden of Guangxi Zhuang Autonomous Region; the cultivation temperature of Millettia reticulata is 25 °C, and the relative air humidity for cultivation is 70%.

[0053] The LED spectral lighting method uses a light quality ratio of red light to blue light of 5:1, the total photon flux density of red light and blue light is 200 μmol / m2 / s, and the light cycle is 16 h / d.

[0054] The growth regulator is a methyl jasmonate solution. The methyl jasmonate solution is prepared with 1 mL of absolute ethanol as the solvent, and the solution concentration is 100 μmol / L.

[0055] The specific spraying amount of the growth regulator sprayed on the leaves of Millettia reticulata is 30 mL per plant in total. It is sprayed at 9 am every day, and the time interval between each spraying is 7 days.

[0056] Six biological replicates are set for each treatment. Observe the morphological changes of Millettia reticulata and sample every 15 days. After 60 days of conventional management, measure the content of active ingredients in Millettia reticulata uniformly.

[0057] Example 5

[0058] This example provides a method for improving the quality of Millettia reticulata by combining a growth regulator with LED spectral lighting, including spraying the growth regulator on the leaves of Millettia reticulata and simultaneously combining with LED spectral lighting. Select Millettia reticulata plants with similar and good growth as test materials and plant them in the plant factory of the Medicinal Plant Garden in Guangxi Zhuang Autonomous Region; the cultivation temperature of Millettia reticulata is 25 °C, and the relative air humidity during cultivation is 70%.

[0059] The LED spectral lighting method uses a light quality ratio of red light to blue light of 2:1, the total photon flux density of red light and blue light is 200 μmol / m 2 / s, and the light cycle is 16 h / d.

[0060] The growth regulator is a methyl jasmonate solution. The methyl jasmonate solution is prepared with 1 mL of absolute ethanol as the solvent, and the solution concentration is 200 μmol / L.

[0061] The specific spraying amount of the growth regulator sprayed on the leaves of Millettia reticulata is 30 mL per plant in total. It is sprayed at 9 am every day, and the time interval between each spraying is 7 days.

[0062] Six biological replicates are set for each treatment. Observe the morphological changes of Millettia reticulata and sample every 15 days. After 60 days of conventional management, measure the content of active ingredients in Millettia reticulata uniformly.

[0063] Example 6

[0064] This example provides a method for improving the quality of Millettia reticulata by combining a growth regulator with LED spectral lighting, including spraying the growth regulator on the leaves of Millettia reticulata while combining with LED spectral lighting. Select Millettia reticulata plants with similar and good growth as test materials and plant them in the plant factory of the Medicinal Plant Garden in Guangxi Zhuang Autonomous Region; the cultivation temperature of Millettia reticulata is 25 °C, and the relative air humidity for cultivation is 70%.

[0065] The LED spectral lighting method uses a light quality ratio of red light to blue light of 2:1, and the total photon flux density of red light and blue light is 200 μmol / m 2 / s, and the photoperiod is 16 h / d.

[0066] The growth regulator is a methyl jasmonate solution, and the methyl jasmonate solution is prepared with 1 mL of absolute ethanol as the solvent, and the solution concentration is 300 μmol / L.

[0067] The specific spraying amount of the growth regulator sprayed on the leaves of Millettia reticulata is 30 mL per plant in total, sprayed at 9 am every day, and the spraying time interval for each time is 7 d.

[0068] Six biological replicates are set for each treatment, and the morphological changes of Millettia reticulata are observed and samples are taken every 15 d. After 60 d of conventional management, the contents of active ingredients of Millettia reticulata are measured uniformly.

[0069] Example 7

[0070] This example provides a method for improving the quality of Millettia reticulata by combining a growth regulator with LED spectral lighting, including spraying the growth regulator on the leaves of Millettia reticulata while combining with LED spectral lighting. Select Millettia reticulata plants with similar and good growth as test materials and plant them in the plant factory of the Medicinal Plant Garden in Guangxi Zhuang Autonomous Region; the cultivation temperature of Millettia reticulata is 25 °C, and the relative air humidity for cultivation is 70%.

[0071] The LED spectral lighting method uses a light quality ratio of red light to blue light of 2:1, and the total photon flux density of red light and blue light is 200 μmol / m 2 / s, and the photoperiod is 16 h / d.

[0072] The growth regulator is a methyl jasmonate solution, and the methyl jasmonate solution is prepared with 1 mL of absolute ethanol as the solvent, and the solution concentration is 400 μmol / L.

[0073] The specific spraying amount of the growth regulator sprayed on the leaves of Millettia reticulata is 30 mL per plant in total, sprayed at 9 am every day, and the spraying time interval for each time is 7 d.

[0074] Six biological replicates are set for each treatment, and the morphological changes of Millettia reticulata are observed and samples are taken every 15 d. After 60 d of conventional management, the contents of active ingredients of Millettia reticulata are measured uniformly.

[0075] Comparative Example 1

[0076] In this comparative example, Millettia reticulata plants with similar and good growth were selected as test materials and planted in the plant factory of the Medicinal Plant Garden of Guangxi Zhuang Autonomous Region. The cultivation temperature was 25°C, and the relative air humidity for cultivation was 70%.

[0077] Without using growth regulators and LED spectral lighting, 1 mL of absolute ethanol was diluted according to the same multiple and sprayed as the control group (CK) with a sprayer evenly on the leaf surface of Millettia reticulata. The spraying amount per plant was 30 mL, and it was sprayed at 9 am every day, with a time interval of 7 days between each spraying.

[0078] Six biological replicates were set for each treatment. The morphological changes of Millettia reticulata were observed and samples were taken every 15 days. After 60 days of conventional management, the contents of active ingredients in Millettia reticulata were measured uniformly.

[0079] Comparative Example 2

[0080] In this comparative example, Millettia reticulata plants with similar and good growth were selected as test materials and planted in the plant factory of the Medicinal Plant Garden of Guangxi Zhuang Autonomous Region. The cultivation temperature was 25°C, and the relative air humidity for cultivation was 70%.

[0081] The LED spectral lighting method used a light quality ratio of red light to blue light of 2:1, the total photon flux density of red light and blue light was 200 μmol / m 2 / s, and the photoperiod was 16 h / d.

[0082] Without using growth regulators, 1 mL of absolute ethanol was diluted with water according to the same multiple and sprayed with a sprayer evenly on the leaf surface of Millettia reticulata. The spraying amount per plant was 30 mL, and it was sprayed at 9 am every day, with a time interval of 7 days between each spraying.

[0083] Six biological replicates were set for each treatment. The morphological changes of Millettia reticulata were observed and samples were taken every 15 days. After 60 days of conventional management, the contents of active ingredients in Millettia reticulata were measured uniformly.

[0084] Comparative Example 3

[0085] In this comparative example, Millettia reticulata plants with similar and good growth were selected as test materials and planted in the plant factory of the Medicinal Plant Garden of Guangxi Zhuang Autonomous Region. The cultivation temperature was 25°C, and the relative air humidity for cultivation was 70%.

[0086] The LED spectral lighting method used a light quality ratio of red light to blue light of 2:1, the total photon flux density of red light and blue light was 100 μmol / m 2 / s, and the photoperiod was 16 h / d.

[0087] Without using growth regulators, dilute with water in the same multiple according to 1 mL of absolute ethanol, spray with a sprayer evenly on the surface of Millettia reticulata leaves, the spraying amount per plant is 30 mL, spray at 9 am every day, and the time interval between each spraying is 7 days.

[0088] Set 6 biological replicates for each treatment, observe the morphological changes of Millettia reticulata and sample every 15 days. After 60 days of conventional management, uniformly determine the content of active ingredients in Millettia reticulata.

[0089] Control Example 4

[0090] In this control example, select Millettia reticulata plants with similar and good growth as test materials, plant them in the plant factory of Guangxi Medicinal Botanical Garden, the cultivation temperature is 25 °C, and the relative humidity of the cultivation air is 70%.

[0091] The LED spectral lighting method uses a light quality ratio of red light to blue light of 2:1, the total photon flux density of red light and blue light is 400 μmol / m 2 / s, and the photoperiod is 16 h / d.

[0092] Without using growth regulators, dilute with water in the same multiple according to 1 mL of absolute ethanol, spray with a sprayer evenly on the surface of Millettia reticulata leaves, the spraying amount per plant is 30 mL, spray at 9 am every day, and the time interval between each spraying is 7 days.

[0093] Set 6 biological replicates for each treatment, observe the morphological changes of Millettia reticulata and sample every 15 days. After 60 days of conventional management, uniformly determine the content of active ingredients in Millettia reticulata.

[0094] Control Example 5

[0095] In this control example, select Millettia reticulata plants with similar and good growth as test materials, plant them in the plant factory of Guangxi Medicinal Botanical Garden, the cultivation temperature is 25 °C, and the relative humidity of the cultivation air is 70%.

[0096] Do not use LED spectral lighting.

[0097] The growth regulator is methyl jasmonate solution, prepare methyl jasmonate solution with 1 mL of absolute ethanol as the solvent, and the solution concentration is 200 μmol / L.

[0098] The specific spraying amount of the growth regulator sprayed on the leaves of Millettia reticulata is 30 mL per plant in total, spray at 9 am every day, and the time interval between each spraying is 7 days.

[0099] Set 6 biological replicates for each treatment, observe the morphological changes of Millettia reticulata and sample every 15 days. After 60 days of conventional management, uniformly determine the content of active ingredients in Millettia reticulata

[0100] Control Example 6

[0101] In this comparative example, Millettia reticulata plants with similar and good growth were selected as test materials and planted in the plant factory of the Medicinal Plant Garden of Guangxi Zhuang Autonomous Region. The cultivation temperature was 25°C, and the relative air humidity during cultivation was 70%.

[0102] LED spectral lighting was not used.

[0103] The growth regulator was a gibberellin solution. A gibberellin solution was prepared with 1 mL of absolute ethanol as the solvent, and the solution concentration was 200 μmol / L.

[0104] The specific spraying amount of the growth regulator on the leaves of Millettia reticulata was 30 mL per plant in total. It was sprayed at 9 am every day, and the time interval between each spraying was 7 days.

[0105] Six biological replicates were set for each treatment. The morphological changes of Millettia reticulata were observed and samples were taken every 15 days. After 60 days of conventional management, the contents of active ingredients in Millettia reticulata were measured uniformly.

[0106] Comparative Example 7

[0107] In this comparative example, Millettia reticulata plants with similar and good growth were selected as test materials and planted in the plant factory of the Medicinal Plant Garden of Guangxi Zhuang Autonomous Region. The cultivation temperature was 25°C, and the relative air humidity during cultivation was 70%.

[0108] LED spectral lighting was not used in this comparative example.

[0109] The growth regulator was an auxin solution (indole-3-acetic acid). An auxin solution was prepared with 1 mL of absolute ethanol as the solvent, and the solution concentration was 200 μmol / L.

[0110] The specific spraying amount of the growth regulator on the leaves of Millettia reticulata was 30 mL per plant in total. It was sprayed at 9 am every day, and the time interval between each spraying was 7 days.

[0111] Six biological replicates were set for each treatment. The morphological changes of Millettia reticulata were observed and samples were taken every 15 days. After 60 days of conventional management, the contents of active ingredients in Millettia reticulata were measured uniformly.

[0112] The following Table 1 shows the comparison of growth regulators and spectral lighting used in each example and comparative example.

[0113] Table 1 Comparison of spectral lighting and growth regulators in each example

[0114]

[0115]

[0116] Effect analysis

[0117] I.Figure 1 Growth status photo of Example 1

[0118] II. Figure 2 Comparison chart of the contents of total flavonoids, catechin, epicatechin and gallocatechin, the active ingredients of Millettia reticulata Benth. in Example 1, Example 2, Example 3 and Example 4 under different light quality treatments on the 60th day

[0119] It can be seen from Figure 2 that when the red - blue light quality ratio is 2:1 (Example 1), the contents of each active ingredient of Millettia reticulata Benth. are the highest. Compared with the CK group (Comparative Example 1), among them, the contents of total flavonoids, catechin, epicatechin and gallocatechin are significantly increased by 45.47%, 233.17%, 52.33% and 167.01% respectively.

[0120] III. Figure 3 Comparison chart of the contents of total flavonoids, catechin, epicatechin and gallocatechin, the active ingredients of Millettia reticulata Benth. in Comparative Example 1, Example 1, Example 5, Example 6 and Example 7 under different concentrations of growth regulators on the 60th day

[0121] It can be seen from Figure 3 that when the concentration of the growth regulator is 200 μmol / L (Example 5), the contents of each active ingredient of Millettia reticulata Benth. are the highest. Compared with the CK group (Comparative Example 1), among them, the contents of total flavonoids, catechin, epicatechin and gallocatechin are significantly increased by 83.05%, 314.72%, 81.03% and 179.62% respectively.

[0122] IV. Figure 4 Comparison chart of the contents of total flavonoids, catechin, epicatechin and gallocatechin, the active ingredients of Millettia reticulata Benth. in Comparative Example 1, Comparative Example 2, Comparative Example 5 and Example 5 under different growth regulation methods on the 60th day

[0123] It can be seen from Figure 4 that three different methods, namely, using light quality treatment alone (Comparative Example 2), using growth regulator alone (Comparative Example 3), and using light quality treatment and growth regulator in combination (Example 5), can all significantly increase the contents of active ingredients of Millettia reticulata Benth. Among them, when using growth regulator plus light quality treatment for Millettia reticulata Benth. in Example 5, the contents of total flavonoids, catechin, epicatechin and gallocatechin, the active ingredients, are the highest, achieving the effect of efficiently increasing the contents of active ingredients of Millettia reticulata Benth.

[0124] V. Figure 5 Comparison chart of the contents of total flavonoids, catechin, epicatechin and gallocatechin, the active ingredients of Millettia reticulata Benth. in Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 under different total photon flux densities on the 60th day

[0125] It can be seen from Figure 5It can be seen that light quality treatments with three different total photon flux densities can all increase the contents of active ingredients in Millettia reticulata, and when the total photon flux densities of red light and blue light are 200 μmol / m 2 / s are used to treat Millettia reticulata, the contents of the total flavonoids, catechins, epicatechins and gallocatechins, which are the active ingredients, are the highest.

[0126] VI. Figure 6 It is a comparison chart of the contents of the total flavonoids, catechins, epicatechins and gallocatechins, which are the active ingredients, of Millettia reticulata in Comparative Example 1, Comparative Example 5, Comparative Example 6 and Comparative Example 7 on the 60th day under different growth regulators.

[0127] From Figure 6 it can be seen that treatments with three different growth regulators can all increase the contents of active ingredients in Millettia reticulata to a certain extent; when Millettia reticulata is treated with the growth regulator methyl jasmonate, the contents of the total flavonoids, catechins, epicatechins and gallocatechins, which are the active ingredients, are the highest, and there is a significant overall improvement effect on the active ingredients, and the improvement effect on the catechin content is the most obvious; when gibberellin and auxin are used as growth regulators, the improvement effects on the total flavonoids and epicatechins are slightly weaker, that is, the overall improvement effect on the active ingredients is weaker, and only the improvement on the catechins and gallocatechins is relatively significant.

[0128] In summary, it can be seen that the combination of the light quality treatment and the growth regulator of the present invention can improve the contents of the total flavonoids, catechins, epicatechins and gallocatechins in Millettia reticulata, that is, there is an overall improvement effect, among which the improvement effect on the catechins is the most obvious, and the improvement effect on the gallocatechins is the second. Therefore, the method of the present invention can be applied to improving the overall contents of the total flavonoids, catechins, epicatechins and gallocatechins, and can also be applied to improving the contents of single ingredients such as catechins.

[0129] Therefore, the present invention uses methyl jasmonate as the growth regulator to stimulate the accumulation of active ingredients in Millettia reticulata, and at the same time cooperates with the LED spectrum illumination with different red and blue light quality ratios as the light source to give full play to the advantages of the environmental control of the plant factory, so as to realize the rapid and high-quality growth of Millettia reticulata under artificial intelligence conditions and significantly improve the contents of active ingredients, and greatly improve the quality of the product.

[0130] Although the embodiments of the present invention have been disclosed as above, they are not limited to the applications listed in the specification and the embodiments. It can be fully applied to various fields suitable for the present invention. For those familiar with the field, additional modifications can be easily achieved.

Claims

1. A method for improving the quality of Millettia reticulata, characterized in that Apply a growth regulator to the Millettia reticulata plant while cooperating with spectral lighting. The LED light quality of the spectral lighting is red light and blue light, and the total photon flux density of the red light and the blue light is 200 μmol / m 2 / s, and the light quality ratio of the red light and the blue light is 2:1; The growth regulator is methyl jasmonate. Methyl jasmonate solution is prepared with absolute ethanol as the solvent, and the concentration of methyl jasmonate is 200 μmol / L; the spraying amount is 30 mL per plant, and the time interval between each spraying is 7 d; the cultivation temperature of Millettia reticulata is 25 °C, and the relative air humidity for cultivation is 70%; Among them, the photoperiod is 16 h / d; the quality of the Millettia reticulata refers to the contents of total flavonoids, catechin, epicatechin and gallocatechin.

2. Use of methyl jasmonate, a growth regulator, in increasing the overall content or the content of any one of total flavonoids, catechin, epicatechin and gallocatechin in Millettia reticulata Benth., wherein, Treat the Millettia reticulata plants according to the method described in Claim 1.

3. Use of a light source system in increasing the overall content or the content of any one substance of total flavonoids, catechin, epicatechin and gallocatechin in Caulis Spatholobi, wherein, The LED light quality of the light source system is red light and blue light, and the total photon flux density of the red light and blue light is 200 μmol / m 2 / s. The light quality ratio of the red light to the blue light is 2:1, and the millettia reticulata plants are treated according to the method described in claim 1.

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