Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

38 results about "Dehydroascorbic acid" patented technology

Dehydroascorbic acid (DHA) is an oxidized form of ascorbic acid (vitamin C). It is actively imported into the endoplasmic reticulum of cells via glucose transporters. It is trapped therein by reduction back to ascorbate by glutathione and other thiols. The (free) chemical radical semidehydroascorbic acid (SDA) also belongs to the group of oxidized ascorbic acids.

Method for synchronously detecting L-ascorbic acid, D-ascorbic acid and dehydroascorbic acid in milk powder

The invention provides a method for synchronously detecting L-ascorbic acid, D-ascorbic acid and dehydroascorbic acid in milk powder. According to the method, deoxidized sodium thiosulfate and metaphosphoric acid mixed solution is used as an extracting liquid, active ascorbic acid can be effectively protected, the stability of the ascorbic acid can be improved, light-resistant operation is not required, experimental operation is simple and convenient, and the extract has stable property in 24 hours at low temperature. The chromatographic condition has small damage to a chromatographic column,and the service life of the chromatographic column can be prolonged; a mobile phase is formed by monopotassium phosphate and decyl amine, the resolution can be improved by the concentration of the mobile phase, two geometric isomers can still be effectively separated when the ascorbic acid has relatively high concentration, mutual interference can be avoided, and the content of active ascorbic acid can be accurately detected. The method has the advantages of simple operation, small chemical reagent dosage and good extract stability, has a relative standard deviation of 7.82 percent, can be used for multi-union detection, and has an important meaning for accurately detecting the true content of active ascorbic acid in milk powder.
Owner:山东世通检测评价技术服务有限公司 +1

Method for simultaneously determining content of reduced ascorbic acid, dehydroascorbic acid and total ascorbic acid in fruit

InactiveCN105758815AStable standard curveColor/spectral properties measurementsAntioxidantThiourea
The invention discloses a method for simultaneously determining content of reduced ascorbic acid, dehydroascorbic acid and total ascorbic acid in fruit. The method comprises the following steps: adding oxalic acid into a fresh fruit to extract the total ascorbic acid; separating extracting solution into two portions; adding copper sulfate into one portion for oxidization, and determining the content of the total ascorbic acid by means of a 2,4-dinitrophenylhydrazine colorimetric method; adding an antioxidant, thiourea into the other portion, and determining the content of the dehydroascorbic acid by means of the 2,4-dinitrophenylhydrazine colorimetric method; oxidizing the reduced ascorbic acid in a test sample into the dehydroascorbic acid by activated carbon, then adding 2,4-dinitrophenylhydrazine to generate red osazone, and performing colorimetric determination at a position of 500nm; deducting the obtained content of the total ascorbic acid from the obtained content of the dehydroascorbic acid before the oxidization to obtain the content of the reduced ascorbic acid. According to method disclosed by the invention, the contents of three different kinds of ascorbic acids in fruits such as apple and pear are determined, a standard curve is stable, a linear scope is 1-10mu. g / mL, and a correlation coefficient reaches 0.99 or more.
Owner:FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products