8 results about "Oxidized Ascorbic Acid" patented technology

The application belongs to the technical field of nanomaterials, and provides ascorbic acid oxidation mimetic enzyme and preparation thereof. The application adopts a methanol solution of zinc nitrate, copper acetylacetonate and 2-methyl imidazole to perform a hydrothermal reaction, and obtains the ascorbic acid oxidation mimetic enzyme by oxygen isolation calcination of a hydrothermal product. The copper-nitrogen-carbon nanomaterial obtained by the application has a rhombohedron structure, can specifically oxidize ascorbic acid, and can be applied to ascorbic acid content detection, oxidized ascorbic acid preparation and dehydroascorbic acid preparation as ascorbic acid oxidation enzyme mimetic enzyme. The nanomaterial can maintain ascorbic acid oxidation mimetic enzyme activity under acid, alkaline and high temperature conditions; the steady-state kinetic parameter (Michaelis constant is 0.012 mM) is better than that of natural ascorbic acid oxidation enzyme, and the ascorbic acid oxidation mimetic enzyme activity of the material has a broad application prospect in the fields of nanozyme, nanotechnology and biological analysis detection.

Methods for the manufacture of disulfide bonded peptides are provided. These methods rely on the use of dehydroascorbic acid as oxidizing agent and are advantageous in that they are applicable to aggregation-prone peptides, reduce the formation of unwanted side products, avoid working with highly diluted samples of peptide and oxidizing agent, and thereby allow for economic as well as environmentally friendly production.

The invention provides a whole blood treatment agent, a kit and application of the whole blood treatment agent in detection of calprotectin, and relates to the technical field of in-vitro detection, the whole blood treatment agent comprises 5-15 mmoL / ml of ammonium chloride or 0.01-0.05 mg / ml of ascorbic acid oxidase, and a solvent is an enzyme diluent. The enzyme diluent is used as a solvent, so that the whole blood treatment agent maintains a stable environment in a sample treatment process, and related enzyme activity is maintained. Ammonium chloride promotes erythrocyte lysis to release hemoglobin; ascorbic acid oxidase can catalyze ascorbic acid to be oxidized into dehydroascorbic acid, and interference factors are reduced. By using the whole blood treatment agent, a tedious pretreatment step on a whole blood sample by using a sample treatment liquid is not needed, the directly collected whole blood sample can be used for subsequent detection after being mixed with the whole blood treatment agent, and the directly collected whole blood sample can be used for subsequent detection, so that the sample treatment is simplified, and the risk of introducing an error due to the sample treatment is reduced; a detection result accords with a clinical diagnosis result, and the accuracy is high.

This disclosure relates to an apparatus for glucose-sensing that address interference of ascorbic acid and acetaminophen. The apparatus includes a first electrode capable of oxidizing glucose and at least one of ascorbic acid and acetaminophen. The apparatus further includes a second electrode capable of oxidizing at least one of ascorbic acid and acetaminophen but not capable of oxidizing glucose. The first electrode includes a deposit of irregularly shaped bodies that are formed of numerous nanoparticles having a generally oval or spherical shape with a length ranging between about 2 nm and about 5 nm.

The invention belongs to the technical field of lubricating additives, and particularly relates to high-wear-resistance lubricating grease based on a quantum dot rolling ball effect as well as a preparation method and application of the high-wear-resistance lubricating grease. According to the invention, polybasic acid or a bio-based raw material and polyol are used as raw materials for esterification reaction, and ester base oil is independently researched and developed; then ester base oil and quantum dots are compounded, the quantum dots are selected from O-CDS quantum dots, aminated O-CDS dots, phosphate grafted O-CDS quantum dots or O-CDS quantum dots coated with oxidized ascorbic acid, and the high-wear-resistance lubricating grease based on the quantum dot rolling ball effect is obtained. The quantum dots have good compatibility with the ester base oil and are uniformly dispersed in the ester base oil, the preparation cost is low, the ester base oil is prepared without an organic solvent, and the technical defects of lubricating grease in the prior art are overcome.

This invention discloses dehydroascorbic acid-modified hemoglobin-based Paris polyphylla saponin-targeted nanoparticles and their preparation method, belonging to the field of drug delivery system technology. These targeted nanoparticles use hemoglobin as a drug carrier and Paris polyphylla saponin VII as the core active ingredient. The process involves first synthesizing a DSPE-PEG-DHA polymer, then utilizing the property of Hb exposing its hydrophobic region under acidic conditions to encapsulate PSVII within Hb via a self-assembly method, and finally modifying it with DSPE-PEG-DHA to obtain the target nanoparticles PSHb@DPD. This invention significantly enhances the targeting and cellular uptake efficiency of nanoparticles by specifically binding DHA to glucose transporters highly expressed in osteosarcoma cells. It exhibits strong toxicity to osteosarcoma MG63 cells and effectively inhibits their migration, while showing no significant toxicity to normal vascular endothelial cells and excellent biocompatibility.

The present invention relates to methods and kits for radiolabelling a chelate-functionalized targeting agent with a metal radionuclide being gallium-68, using a stabiliser that prevents radiolysis (product degradation) of the chelate-functionalized targeting agent, wherein said stabiliser is selected from the group consisting of: ascorbic acid, dehydroascorbic acid, gentisic acid, cysteine and methionine, sodium ascorbate, or a salt thereof, preferably as a solution. The invention further relates to the use of said stabilizer against radiolytic decomposition of a radiolabelled chelate-functionalized targeting agent composition and methods of detecting a prostate tumour or cancer using the method for radiolabelling.

The invention discloses a ratiometric fluorescence system based on o-phenylenediamine and sodium ascorbate and detection of copper ions in water by the ratiometric fluorescence system. In the ratiometric fluorescence system, Cu < 2 + > oxidizes sodium ascorbate to generate dehydroascorbic acid, and then the dehydroascorbic acid reacts with o-phenylenediamine to generate quinoxaline; in addition, Cu < 2 + > and o-phenylenediamine can be subjected to a redox reaction to generate 2, 3-diaminophenazine. Two products generated by the reaction, namely quinoxaline and 2, 3-diaminophenazine, respectively generate fluorescence emission peaks at 435nm and 560nm, and a fluorescence emission peak intensity ratio (F560 / F435) is used as an analysis signal, so that background interference can be effectively eliminated, and a detection result is more accurate. The method does not need to prepare a nano material, and is simpler and more convenient.