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41 results about "Oxidized Ascorbic Acid" patented technology

Dehydroascorbic acid (DHA) is an oxidized form of ascorbic acid (vitamin C). It is actively imported into the endoplasmic reticulum of cells via glucose transporters. It is trapped therein by reduction back to ascorbate by glutathione and other thiols.

Environment-sensitive tumor-targeting polymer micelle and preparation method thereof

The invention belongs to the technical field of biology, relates to environment-sensitive tumor-targeting polymer micelle and a preparation method thereof, and especially relates to a cell microenvironment-sensitive oxidized ascorbic acid-modified tumor-targeting polymer micelle administration system and a preparation method thereof. An administration system realizes delivery in tumor by a glucose transporter mechanism, the polymer micelle of which the inner core has a disulfide bond crosslinked barrier is designed by use of the GSH characteristics of high density in the cell microenvironment, drug release characteristics trigged by the cell microenvironment are obtained, and the environment-sensitive tumor-targeting polymer micelle has a high transport capability, a fast transport rate and difficult saturation. Compared with polypeptide protein molecules, the environment-sensitive tumor-targeting polymer micelle has the characteristics of simple synthesis preparation, high stability and no immunogenicity. Through synergism of targeting and structure stabilization, drug delivery to a target position and a target cell is realized so that drug delivery efficiency is greatly improved and thus the environment-sensitive tumor-targeting polymer micelle has a good clinical application prospect.
Owner:FUDAN UNIV

Green and environment-friendly conductive nano-copper ink and preparation method thereof

The invention discloses green and environment-friendly conductive nano-copper ink and a preparation method thereof. The preparation method utilizes cavitation and acoustic flow effects of high-energyultrasonic waves in a liquid phase to change a chemical reaction mechanism so as to efficiently obtain nano-copper particles, and the preparation method specifically comprises the following steps thatfirstly, using ascorbic acid as a reducing agent, taking copper hydroxide as a copper source, then using the dispersion effect of dehydroascorbic acid which is a product generated after ascorbic acidreaction, combining a special effect of a ultrasonic wave in the liquid phase, and therefore efficiently synthesizing a required copper nanoparticle solution; and then, separating and obtaining purecopper nanoparticles from the copper nanoparticle dispersion liquid, after repeatedly washing, adding various organic solvents, and mixing to obtain the nano-copper conductive ink. The preparation method has the advantages of unique reaction mechanism, novel preparation means, simple process, low cost, high yield efficiency, environmental friendliness, easiness in large-scale production and the like, and has a wide application prospect in the field of printed electronics.
Owner:HARBIN INSTITUTE OF TECHNOLOGY SHENZHEN (INSTITUTE OF SCIENCE AND TECHNOLOGY INNOVATION HARBIN INSTITUTE OF TECHNOLOGY SHENZHEN)

Method for synchronously detecting L-ascorbic acid, D-ascorbic acid and dehydroascorbic acid in milk powder

The invention provides a method for synchronously detecting L-ascorbic acid, D-ascorbic acid and dehydroascorbic acid in milk powder. According to the method, deoxidized sodium thiosulfate and metaphosphoric acid mixed solution is used as an extracting liquid, active ascorbic acid can be effectively protected, the stability of the ascorbic acid can be improved, light-resistant operation is not required, experimental operation is simple and convenient, and the extract has stable property in 24 hours at low temperature. The chromatographic condition has small damage to a chromatographic column,and the service life of the chromatographic column can be prolonged; a mobile phase is formed by monopotassium phosphate and decyl amine, the resolution can be improved by the concentration of the mobile phase, two geometric isomers can still be effectively separated when the ascorbic acid has relatively high concentration, mutual interference can be avoided, and the content of active ascorbic acid can be accurately detected. The method has the advantages of simple operation, small chemical reagent dosage and good extract stability, has a relative standard deviation of 7.82 percent, can be used for multi-union detection, and has an important meaning for accurately detecting the true content of active ascorbic acid in milk powder.
Owner:山东世通检测评价技术服务有限公司 +1

Method for simultaneously determining content of reduced ascorbic acid, dehydroascorbic acid and total ascorbic acid in fruit

InactiveCN105758815AStable standard curveColor/spectral properties measurementsAntioxidantThiourea
The invention discloses a method for simultaneously determining content of reduced ascorbic acid, dehydroascorbic acid and total ascorbic acid in fruit. The method comprises the following steps: adding oxalic acid into a fresh fruit to extract the total ascorbic acid; separating extracting solution into two portions; adding copper sulfate into one portion for oxidization, and determining the content of the total ascorbic acid by means of a 2,4-dinitrophenylhydrazine colorimetric method; adding an antioxidant, thiourea into the other portion, and determining the content of the dehydroascorbic acid by means of the 2,4-dinitrophenylhydrazine colorimetric method; oxidizing the reduced ascorbic acid in a test sample into the dehydroascorbic acid by activated carbon, then adding 2,4-dinitrophenylhydrazine to generate red osazone, and performing colorimetric determination at a position of 500nm; deducting the obtained content of the total ascorbic acid from the obtained content of the dehydroascorbic acid before the oxidization to obtain the content of the reduced ascorbic acid. According to method disclosed by the invention, the contents of three different kinds of ascorbic acids in fruits such as apple and pear are determined, a standard curve is stable, a linear scope is 1-10mu. g / mL, and a correlation coefficient reaches 0.99 or more.
Owner:FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
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