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Gas concentration regulator for culturing anaerobic bacteria, and method for culturing anaerobic bacteria using same

A gas concentration, anaerobic bacteria technology, applied in biochemical equipment and methods, methods for stress-stimulated microbial growth, bacteria, etc., can solve the problems of equipment cost and high pressure gas management burden, and achieve the effect of large production volume.

Pending Publication Date: 2022-03-01
MITSUBISHI GAS CHEM CO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Carbon dioxide gas incubators and the like are known as devices for creating a gas environment in a high-concentration carbon dioxide atmosphere and a low-concentration oxygen atmosphere, but the burden of equipment cost and high-pressure gas management is large

Method used

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Examples

Experimental program
Comparison scheme
Effect test

manufacture example 1

[0096] (Preparation of Ascorbic Acid Aqueous Solution)

[0097] Under a nitrogen atmosphere, 30.6 g of L-ascorbic acid, 46.2 g of a 15% by mass sodium hydroxide aqueous solution, and 4.8 g of ferrous sulfate heptahydrate were mixed to prepare a 2.13 mmol / g aqueous ascorbic acid solution.

manufacture example 2

[0099] (Preparation of dehydroascorbic acid aqueous solution)

[0100] Under a nitrogen atmosphere, 4.5 g of dehydroascorbic acid, 6.9 g of a 15% by mass sodium hydroxide aqueous solution, and 0.7 g of ferrous sulfate heptahydrate were mixed to prepare a 2.13 mmol / g dehydroascorbic acid aqueous solution.

Embodiment 1

[0102] Under a nitrogen atmosphere, 10 g of activated carbon, 15 g of polyethylene powder, and 4.7 g of sodium carbonate were weighed and mixed. To this powder, 10.1 g of an aqueous solution of ascorbic acid and 10.1 g of an aqueous solution of dehydroascorbic acid were added and mixed to manufacture a deoxidizer composition. 6.9 g of this deoxidizer composition was filled and sealed in an aluminum bag.

[0103] Seal the aluminum bag containing the above-mentioned deoxidizer together with 750 mL of air into a nylon bag, unseal the aluminum bag in the nylon bag, and start the deoxidation experiment. The oxygen concentration and the carbon dioxide concentration after 3 hours were measured by gas chromatography, and the oxygen concentration was 0.20% by volume and the carbon dioxide concentration was 13.6% by volume. In addition, when the oxygen concentration and the carbon dioxide concentration after 24 hours were measured by gas chromatography, the oxygen concentration was 0.0...

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Abstract

A gas concentration regulator for culturing anaerobic bacteria, comprising: (a) dehydroascorbic acid; (c) a transition metal catalyst; (d) activated carbon; (e) at least one element selected from the group consisting of alkali metal carbonates, alkali metal hydroxides, and alkaline earth metal hydroxides; and (f) water.

Description

technical field [0001] The present invention relates to a gas concentration regulator for cultivating anaerobic bacteria and a method for cultivating anaerobic bacteria using the same. Background technique [0002] In the cultivation of biological samples such as tissues / cells carried out in the research field of biology, reproduction, or biotechnology or in the industrial field, a gas environment different from the atmospheric atmosphere is required. For example, as a condition for maintaining the pH of the bicarbonate-buffered culture solution at pH 7.4, which is the same as the normal state of blood, it is necessary to set the atmospheric carbon dioxide concentration to about 5%. In addition, in many research fields, cell culture is performed under the same low-concentration oxygen atmosphere as in vivo. [0003] Carbon dioxide gas incubators and the like are known as devices for creating a gas environment in a high-concentration carbon dioxide atmosphere and a low-conce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/00B65D81/26
CPCC12N1/20C12N1/00B65D81/26C12M41/34C12M35/08
Inventor 镇西达也染谷昌男
Owner MITSUBISHI GAS CHEM CO INC
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