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Nano copper oxide with activity of ascorbic acid oxidization mimic enzyme

A technology of nano-copper oxide and ascorbic acid, which is applied in the field of nanotechnology and bionics, and can solve problems such as harsh operating conditions, application limitations, and inactivation of ascorbic acid oxidase

Inactive Publication Date: 2019-09-03
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, similar to many natural enzymes, ascorbate oxidase is easily affected by many factors and loses its activity. In practical applications, the operating conditions of related experiments are relatively harsh, which greatly limits its application.
[0004] Since the peroxide nanozyme was reported, although more and more nanozymes have been invented and reported one after another, the ascorbic acid oxidation mimetic enzyme activity of nanomaterials still needs to be studied and developed.

Method used

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  • Nano copper oxide with activity of ascorbic acid oxidization mimic enzyme
  • Nano copper oxide with activity of ascorbic acid oxidization mimic enzyme
  • Nano copper oxide with activity of ascorbic acid oxidization mimic enzyme

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0041] The specific preparation steps of nano-copper oxide are as follows: (1) Take 150 ml of 0.02 mol / L copper acetate solution and 0.5 ml of glacial acetic acid into a three-necked flask equipped with a condenser, stir and heat to boiling; (2) Quickly add 0.04 g / ml sodium hydroxide solution 10 ml, continue to stir for 5 minutes after the addition, to obtain a brown copper oxide precipitate; (3) centrifuge the black copper oxide precipitate obtained from the reaction, wash with absolute ethanol three times, and dry under reduced pressure, that is A nano-copper oxide powder with a diameter of 6 nm was obtained.

example 2

[0043] In the presence of oxygen, the ascorbic acid oxidation mimetic enzyme activity of nano-copper oxide was investigated. Add 50 µL of nano-copper oxide (40 mg / L) prepared in Example 1 and 0.5 mL of ascorbic acid (1 mM) to 3.45 mL of phosphate buffer (pH 7.0, 200 mM) at a water bath temperature of 45 °C, and react After 6 minutes, measure the ultraviolet-visible absorption spectrum and the absorbance at a wavelength of 265 nm with an ultraviolet spectrophotometer. Such as figure 1 As shown, after adding nano-copper oxide to the reaction, as the reaction time prolongs, the absorbance of the system at a wavelength of 265 nm decreases significantly, indicating that ascorbic acid in the system is obviously consumed.

example 3

[0045] The ascorbic acid oxidation mimetic enzyme activity of nano-copper oxide was investigated after 20 minutes of nitrogen gas and in the presence of oxygen. Add 50 µL of nano-copper oxide (40 mg / L) prepared in Example 1 and 0.5 mL of ascorbic acid (1 mM) to 3.45 mL of phosphate buffer (pH 7.0, 200 mM) at a water bath temperature of 45 °C, After reacting for 6 minutes, the ultraviolet-visible absorption spectrum and the absorbance at a wavelength of 265 nm were measured with an ultraviolet spectrophotometer. Such as figure 2 As shown, the absorbance of the system at a wavelength of 265 nm will decrease significantly only in the presence of oxygen and nano-copper oxide at the same time, indicating that nano-copper oxide catalyzes the redox process of ascorbic acid.

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Abstract

The invention discloses a nano copper oxide with the activity of an ascorbic acid oxidization mimic enzyme, and aims at effectively catalyzing ascorbic acid oxidization to generate dehydroascorbic acid under aerobic environment and keeping the activity of the ascorbic acid oxidization mimic enzyme under strong acid, strong base or high temperature. By utilizing an ultraviolet spectrophotometry, the influences, on the nano copper oxide as ascorbic acid oxidase, of the temperature, pH and reaction time are respectively researched. The steady-state kinetic parameters of the nano copper oxide as the ascorbic acid oxidase are equivalent to the natural ascorbic acid oxidase, and the stability of the nano copper oxide as the ascorbic acid oxidase is obviously stronger than the natural ascorbic acid oxidase. By utilizing a deoxidization experiment and a fluorescence analysis method, a mechanism of the nano copper oxide as the ascorbic acid oxidase to catalyze ascorbic acid oxidization is verified. The activity of the ascorbic acid oxidization mimic enzyme shows a relatively tempting application prospect in the technical fields of nano enzymes, nanometers and bionic.

Description

technical field [0001] The invention belongs to the field of nanotechnology and bionic technology. Specifically, a nano copper oxide has the activity of ascorbic acid oxidation mimic enzyme, which can efficiently catalyze the oxidation of ascorbic acid to generate dehydroascorbic acid in an aerobic environment, and can be used in strong acid, strong alkali and high temperature. Both can maintain their ascorbic acid oxidation mimetic enzyme activity. Background technique [0002] Natural enzymes have the characteristics of high catalytic efficiency and substrate specificity. However, because the chemical nature of natural enzymes is protein, they are prone to structural changes and inactivation in complex non-physiological environments such as acid, alkali, and heat. Nanozymes are a class of artificially simulated enzymes that have both the unique properties of nanomaterials and catalytic functions. Compared with natural enzymes, nanozymes have higher catalytic efficiency, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/64C01G3/02B82Y30/00
CPCB82Y30/00C01G3/02G01N21/31G01N21/6428
Inventor 陈伟何少斌胡爱玲邓豪华彭花萍
Owner FUJIAN MEDICAL UNIV
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