The invention relates to a method for extracting leaf
protein from alfafa
hay, aiming at providing a preparation method which can realize
mass production and has the advantages of no
time limit, simple process, low cost and high efficiency. In the method provided by the invention, leaf
protein is extracted by acidification heating method and salt treatment. The method comprises the following steps of: smashing air-dried or
kiln-dried alfalfa, adding appropriate amount of
sodium pyrosulphate, adding water in the ratio of material to water being 1: (10-40), preimpregnating for 12-36 hours and then beating for 5 minutes, filtering (or centrifuging) with a 120-mesh filter cloth to obtain green juice, adjusting pH value to 2-7 by utilizing 2%
sodium hydroxide and 0.5 mol / L
hydrochloric acid solution, rapidly heating to the temperature of 40-80 DEG C in a boiling water bath, and then flocculating for 3-20 minutes in a constant-temperature water bath at the same temperature; and rapidly cooling to
room temperature with cold water (normal temperature), standing for 1 hour, filtering (or centrifuging) to obtain leaf
protein paste, and finally
drying in a
drying oven at the temperature of 60-70 DEG C. In the extraction method, the leaf protein is extracted from alfafa
hay used as
raw material; compared with the traditional method which takes fresh alfafa
hay as the
raw material, the cost is reduced, the
yield efficiency is greatly improved, and the defect that the production time is limited by
plant growth season is overcome.