The invention discloses a manufacturing method of ramie based on sectioned circulation, belonging to the manufacturing method of textile fibre and solving the problems that the prior method has a large quantity of waste water, serious pollution and high cost. The method comprises following steps: pretreatment of primary ramie, chemical treatment, biological treatment and post treatment; the pretreatment improves the degree of looseness of the primary ramie through beating, ultrasound or extraction; the biological treatment degrades pectin, hemicellulose and others impurities through bio-enzyme or microbial fermentation; the chemical treatment further degrades residual pectin and others impurities through alkali boil-off; the post treatment obtains refined dry ramie by beating, whitening, water scrubbing, dehydration, oil feeding, drying, carding and baking. The quality of the product meets the requirements of first-class GB/T 20793-2006, the degumming yield rate is improved by 15%, the water consumption is reduced by at least 60%, the method is free from using acid, the alkali charge is reduced by 65%; water, the alkali and the bio-enzyme used in each step are recyclable; the byproducts can be recycled in time, the sewage quantity is small and the pollution level is low.