The invention relates to a mucor circinelloides DK1 strain and an application thereof. An ITS (internal transcribed spacer) sequence of the strain is shown by SEQ ID NO:1. The strain is applied to preparation of flax, jute, kenaf or bamboo fiber by retting, boiling-off of flax, jute, kenaf or bamboo yarns, and degumming of flax, jute, kenaf original hemp or bamboo fabrics. The strain provided by the invention is short in growth cycle, cannot be polluted easily, is low in treatment cost, good in fiber quality after treatment, mild in treatment environment, good in heat resistance and free from environmental pollution; and the strain can be directly applied to degumming of the flax, jute, kenaf and bamboo fibers, and is short in degumming cycle, high in fiber dispersion rate and high in degumming efficiency.