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43results about How to "Short degumming cycle" patented technology

Epicoccum nigrum DB3 bacterial strain as well as preparation and application thereof

The invention relates to an epicoccum nigrum DB3 bacterial strain as well as a preparation and an application thereof. The preparation method comprises the steps of: 1, placing rotten sea grass in an enrichment culture medium, standing and culturing at room temperature, then coating the enrichment culture medium in a separation culture medium, standing and culturing at a temperature of 30 DEG C for 1-4 days to obtain a wild type flax biological treatment bacterial strain; 2, inoculating the bacterial strain obtained from the step 1 into a flax lignin nutrition culture medium, culturing at a temperature of 28 DEG C for 72 hours; and 3, selecting the bacterial strain with the capacity of degrading the lignin from the flax lignin nutrition culture medium to obtain the epicoccum nigrum DB3 bacterial strain. The epicoccum nigrum DB3 bacterial strain can be applied to a process for preparing spinning flax, hemp, jute or red ramie fibers through peroxide degumming. The epicoccum nigrum DB3 bacterial strain has the characteristics of short growth period, low possibility of being polluted, low treatment cost, mild reaction conditions, strong pollution resistance capacity, good heat-resistant performance, no environment pollution and good quality of the treated fibers. The preparation method has a simple process and is suitable for large-scale industrial production.
Owner:DONGHUA UNIV

Method for preparing laccase by using mucor circinelloides DK1 strain

The invention relates to a method for preparing laccase by using a mucor circinelloides DK1 strain. The method comprises the following steps: (1) transferring the mucor circinelloides DK1 strain from a PDA slant culture medium into a triangular flask filled with a basic liquid medium, carrying out shaking culture, filtering the fermentation liquor to obtain mycelium, washing the mycelium by using sterile water, scattering, collecting the mycelium in the sterile water to prepare mycelium suspension, and preserving the suspension for later use; (2) putting an enzyme production culture medium into the triangular flask, inoculating the mycelium suspension, carrying out shaking culture, periodically extracting the fermentation liquor in the culture process, centrifuging, and taking the supernatant, thereby obtaining the crude enzyme liquor; and (3) centrifuging the crude enzyme liquor, and taking the supernatant, thereby obtaining the laccase. The method disclosed by the invention is simple in process, is suitable for large-scale industrial production and the like, and the prepared laccase can effectively remove non-cellulose colloid in flax, jute, kenaf or bamboo fibers, has the advantages of high degumming efficiency, short degumming period, high fiber dispersion rate, high refined dried-jute quality and the like and has good application prospects.
Owner:DONGHUA UNIV

Method for recycling hemp strain-containing degumming liquid for degumming hemp

The invention relates to a method for recycling hemp strain-containing degumming liquid for degumming hemp, relating to a hemp degumming process method and mainly solving the problems of the traditional hemp degumming method, such as long degumming period, low fiber yield, low hemp fiber strength and large sewage discharge during retting. The method disclosed by the invention comprises the following steps of: activating strains used for degumming hemp; carrying out primary seed culturing; carrying out second-degree seed culturing; carrying out third-degree seed culturing; degumming hemp with strains; recycling hemp strain-containing degumming liquid and adding to a new hemp degumming pond, and then adding hemp stalks, sodium chloride and urea, filling clean water into the hemp degumming pond, increasing the temperature for retting and completing the degumming process when a retting terminal point is reached. By adopting the method disclosed by the invention, 50% of waste water discharge can be reduced every day, energy consumed for degumming hemp is saved by 10%-15%; meanwhile, the degumming period of the hemp stalks is shortened to 80-120h, the hemp fiber yield is improved by 3%-5%, the hemp fiber strength is enhanced to 220-280N, and the quality of hemp fiber is improved.
Owner:HEILONGJIANG UNIV

Microbial degumming process of linen fiber

The invention discloses a microbial degumming process of linen fiber. The microbial degumming process comprises the steps as follows: preparing a bacterial liquid transferring selected strains in a strain culture tank to be cultured continuously for one week to obtain the bacterial solution for later use after the concentration of the bacterial solution meets the requirement; placing the linen fiber in a bleaching jar; spraying the bacterial solution to the bleaching jar in the bath ratio being 1:(7.5-8.5), controlling the pH and temperature in the jar and keeping the constant temperature for45-55 min; after heat preservation, recovering the bacterial solution in the strain culture tank; preparing an acid pickling solution in an auxiliary jar, spraying the acid pickling solution in the bleaching jar, and discharging the solution after circulation for 15-25 min; preparing an oxygen bleaching solution, increasing the temperature to 82-87 DEG C and keeping the constant temperature for 50-70 min; discharging the oxygen bleaching solution and performing washing for 3 times; placing the bleached linen fiber in a dehydrator to be dehydrated for 10-20 min; spreading the dehydrated linen fiber on a radio frequency machine for drying. Few auxiliary materials are used, the stability is good, the degumming period is short, strains can be recycled, the degumming wastewater pollution is reduced, and the cost is reduced.
Owner:TUNGGA LINENANDCOTTON CO LTD

A method for preparing laccase from Candida tropicalis dk2 strain

The invention relates to a method for preparing laccase from candida tropicalis DK2 strains. The method comprises the following steps: (1) transferring the candida tropicalis DK2 strains into a triangular flask containing a basic liquid nutrient medium from a PDA (potato dextrose agar) slant nutrient medium, performing shake culture, filtering fermentation liquor to obtain a mycelium, cleaning the mycelium by using sterilized water, breaking up the mycelium, collecting the mycelium into the sterilized water to prepare a mycelium suspension, and storing for later use; (2) adding an enzyme production nutrient medium into the triangular flask, inoculating the mycelium suspension, performing shake culture, extracting the fermentation liquor regularly during culture, centrifuging, and taking a supernatant as crude enzyme fluid; and (3) centrifuging the crude enzyme fluid, and taking the supernatant to obtain laccase. The method provided by the invention has the characteristics of being simple in process, suitable for large-scale industrial production and the like, and prepared laccase can be used for effectively removing non-cellulose colloids in flax, jute, kenaf or bamboo fiber, has the advantages of high degumming efficiency, short degumming cycle, high fiber dispersion rate, good refined dry flax quality and the like, and has a good application prospect.
Owner:DONGHUA UNIV
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