43results about How to "Short degumming cycle" patented technology

The invention relates to a cellulomonas fimi DA 8 bacterial strain as well as an acquiring method and application thereof. The bacterial strain has the capability of producing pectinase and hemicellulase and has flax degumming activity. The cellulomonas fimi bacterial strain screened by the method has the advantages of large enzyme yield, complete enzyme system, no toxicity, rough culturing condition and the like; after used for flax degumming, the composite degumming enzyme solution not only greatly shortens a degumming period, but also reduces the cost, the fiber splitting degree is improved, and the degumming efficiency reaches 95%. The degumming method requires basically the same equipment required in the traditional warm water degumming method so that the method is especially suitable for improving a process on the basis of the traditional equipment in old enterprises; the cellulomonas fimi bacterial strain screened with the method not only can be used for flax raw-stem degumming, roving scouring, flax fabric biological pretreatment, but also can be used for bast fiber degumming and biological pretreatment of bast fiber plants, such as ramie, jute, hemp and the like.

The invention relates to a method for preparing laccase from candida tropicalis DK2 strains. The method comprises the following steps: (1) transferring the candida tropicalis DK2 strains into a triangular flask containing a basic liquid nutrient medium from a PDA (potato dextrose agar) slant nutrient medium, performing shake culture, filtering fermentation liquor to obtain a mycelium, cleaning the mycelium by using sterilized water, breaking up the mycelium, collecting the mycelium into the sterilized water to prepare a mycelium suspension, and storing for later use; (2) adding an enzyme production nutrient medium into the triangular flask, inoculating the mycelium suspension, performing shake culture, extracting the fermentation liquor regularly during culture, centrifuging, and taking a supernatant as crude enzyme fluid; and (3) centrifuging the crude enzyme fluid, and taking the supernatant to obtain laccase. The method provided by the invention has the characteristics of being simple in process, suitable for large-scale industrial production and the like, and prepared laccase can be used for effectively removing non-cellulose colloids in flax, jute, kenaf or bamboo fiber, has the advantages of high degumming efficiency, short degumming cycle, high fiber dispersion rate, good refined dry flax quality and the like, and has a good application prospect.