Candida tropicalis DK2 strain and application thereof

A technology of Candida tropicalis and strains, applied in the direction of fungi, bacterial retting, biochemical equipment and methods, etc., can solve the problems of high energy consumption of chemical degumming process, waste water cannot be recycled, and large equipment loss, etc., to achieve degumming High efficiency, short degumming cycle, good quality effect

Inactive Publication Date: 2015-05-20
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the scouring methods commonly used in the hemp industry are mainly chemical methods and enzymatic degumming methods. The chemical degumming process consumes a lot of energy and equipment loss, and the discharged wastewater cannot be recycled, causing serious pollution problems.

Method used

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  • Candida tropicalis DK2 strain and application thereof
  • Candida tropicalis DK2 strain and application thereof
  • Candida tropicalis DK2 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The screening method of hemp fiber and bamboo fiber for retting or scouring strains:

[0040] (1) Obtain the rotting hemp and its soil from the retting hemp base, and place the rotting hemp and its soil with the PDA medium in a ratio of 1g:20ml in the PDA medium, and enrich and cultivate for 10 days. Then take 0.1ml PDA culture and spread it on the separation medium, and cultivate it at 30°C for 1-4 days to obtain the wild-type biological treatment high-quality strain.

[0041] (2) Inoculate the strain obtained in step (1) into a PDA medium and cultivate at 30°C for 72 hours. Add freezing buffer and store at -80°C.

[0042] The step (1) PDA culture medium formula is: 200 g potato, 20 g glucose, 15 g agar, 1000 mL distilled water, and natural pH.

[0043] The formula of the step (2) cryopreservation buffer solution per 100ml: 0.1g potassium dihydrogen phosphate, 0.02g dipotassium hydrogen phosphate, 0.06g sodium citrate, 0.03g magnesium sulfate heptahydrate, 12ml glycerol, and ...

Embodiment 2

[0046] Method for preparing laccase using Candida tropicalis DK2 strain:

[0047] (1) Candida tropicalis DK2 strain is stored in PDA medium, and cryopreservation buffer is added;

[0048] (2) In the 50ml PDA culture medium after sterilization and cooling, connect the bacteria ring obtained in step (1), and culture in a shake flask at 200rpm and 30℃ for 24 hours; after culturing the 50ml shake flask, the medium is inoculated Add 5L of flax, jute or kenaf fermentation medium, shake flask culture at 200r / min and 30℃ for 24 hours to obtain fermentation broth;

[0049] (3) Centrifuge the fermentation broth obtained in step (3) at 4° C. and 8000 r / min, and take the supernatant, namely laccase. Then measured by spectrophotometry, the laccase activity is 10-30IU / ml, which is relatively stable at pH5.0-6.0 and 20-60℃.

[0050] Enzyme activity determination method, guaiacol is used as the laccase substrate, and the determination is carried out according to the spectrophotometric method. The la...

Embodiment 3

[0052] The retting process of flax, jute or kenaf is as follows:

[0053] (1) The Candida tropicalis DK2 strain was cultured in a PDA medium (each 1000 ml medium includes 200 g potato, 20 g glucose, 15 g agar, and 1000 mL distilled water), and added cryopreservation buffer. The formula of the cryopreservation buffer per 100ml of the said step: 0.1g potassium dihydrogen phosphate, 0.02g dipotassium hydrogen phosphate, 0.06g sodium citrate, 0.03g magnesium sulfate heptahydrate, 12ml glycerol, add water to make the volume to 100ml, prepare Got.

[0054] (2) Connect a ring of bacteria to 1000ml of flax, jute or kenaf bran culture medium (each 1000ml medium includes 200g flax, jute or kenaf skin, 50g bran, 50ml wort and 950ml water) 1000ml, shake flask Cultivation, culture condition is 30℃, pH is natural, culture time is 24 hours;

[0055] (3) Inoculate the culture medium into the fermentation medium of flax, jute or kenaf bran (each 1000ml medium includes 100g of flax, jute or kenaf sk...

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Abstract

The invention relates to a Candida tropicalis DK2 strain and an application thereof. The ITS sequence of the strain is shown as SEQ ID NO:1. The strain is used for preparing flax, jute, kenaf or bamboo fibers through retting, scouring the flax, jute, kenaf or bamboo yarns and degumming the original hemp of flax, jute and kenaf or bamboo fabrics. The strain disclosed by the invention is short in growth period and is difficultly polluted, the treatment cost is low, the treated fibers are high in quality, the treatment environment is mild, the heat resistance is high, and environmental pollution is avoided; and moreover, the strain can be directly applied to degumming the flax, jute, kenaf or bamboo fibers and has the advantages of short degumming period, high fiber dispersion rate and high degumming efficiency.

Description

Technical field [0001] The invention belongs to the technical field of biological textiles, and particularly relates to a Candida tropicalis DK2 strain and its application. Background technique [0002] Pseudo-dead yeast is a kind of rot parasite, which is widely found in nature and can be isolated from fruits, vegetables, dairy products, and soil. [0003] In the initial processing of hemp, it is necessary to pre-process part of the gum (mainly pectin and hemicellulose) through a retting process. The retting process actually uses traditional microbial technology, which is a process of slowly removing non-cellulose gum attached to the fiber surface through the synergistic action of microorganisms and enzymes. The retting process has a great influence on the quality of hemp fiber. Commonly used retting methods include rain dew retting and enzymatic retting. [0004] In addition, in textile processing, it must be further subjected to scouring and degumming, that is, to remove pectin,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16D01C1/00C12R1/74
CPCC12N1/165C12R2001/74D01C1/04D10B2201/01
Inventor 丁若垚郁崇文张兴群郭营李晓龙关赛鹏
Owner DONGHUA UNIV
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