2146results about How to "Not easy to pollute" patented technology

The invention relates to a film coating agent containing steroid hormone, which comprises 1 part of steroid hormone medicament, 1-50 parts of film forming materials, 0.5-10 parts of plasticizer, 0.5-10 parts of biological adhesive materials and 1-50 volumes of transdermal enhancer. The preparation of the present invention is capable of raising the efficiency of conveying steroid hormone medicament into the body by permeating skin and improving the irritation to the skin, and has good survivability. The invention also relates to a preparation method of the film coating agent, and the preparation method has the advantages of simple process, convenient operation and less energy consumption.

The invention discloses a cultivating and producing method for haematococcus pluvialis. A haematococcus pluvialis green swarm cell is inoculated to a culture medium in a sealed tank body, a manual light-emitting diode (LED) light source is utilized for irradiation cultivating, the wavelength range of a monochromatic light emitted by the LED light source is 450nm to 640nm with the bandwidth of 30nm, two types of light sources with different wavelengths are simultaneously used according to the proportion of light intensity of red lights and blue lights, which is in a range from 2:1 to 5:1, the total light intensity is in a range from 80mu E / m2.s to 1500mu E / m2.s, the temperature during the cultivating process is maintained in a range from 15 DEG C to 28 DEG C, and the potential of hydrogen (pH) of the cultivating environment is controlled in a range from 8.0+ / -1.0 by adding carbon dioxide. The energy consumption of temperature controlling is remarkably saved, the cultivating period is shortened by above 50% compared with that of traditional methods, and the concentration of the obtained haematococcus pluvialis cell in a green algae liquid is 2 to 5 times of that of the haematococcus pluvialis cell in a green algae liquid by traditional methods.

The invention relates to a comprehensive treatment method for garbage leachate. The comprehensive treatment method for the garbage leachate comprises the following steps: (1) performing foam separation pretreatment; (2) performing coagulation sedimentation pretreatment; (3) performing microfiltration and ultrafiltration treatment; (4) performing gaseous film separation treatment; and (5) performing biochemical treatment. The garbage leachate is subjected to coagulation sedimentation and foam separation pretreatment, so that most of metal ions, floating oil, surfactants and part of organic matters are removed from the garbage leachate and the chemical oxygen demand (COD) and the chromaticity of the garbage leachate are reduced; suspended matters, macromolecular matters and the like are removed from the leachate through the microfiltration and ultrafiltration after pretreatment, so the liquid is clear, the gaseous film is difficult to block and pollute, and long-term operating stabilityof the gaseous film in the subsequence process is guaranteed; and ammonia nitrogen is efficiently removed from the leachate in the gaseous film stabilizing process and the ammonia nitrogen removal rate can be adjusted as required, so that the waste water has the best nitrogen carbon ratio and the successful operation of the subsequence biochemical system is guaranteed.

A thermal phase-separating process for preparing millipore membrane of hollow polypropylene fibres from polypropylene includes preparing spinning solution from diluent (plant oil, atoleine, diphenyl ether and machine oil), assistant (adipic acid and benzoic acid) and polypropylene, heating, spinning, cooling, extracting diluent and recovering diluent. The pore diameter of resultant membrane is 0.01-0.1 micron, and is easily controlled.

The invention provides an oil-water separation fiber membrane with an excellent anti-pollution capability and a preparation method thereof and belongs to the technical field of new materials. Polyacrylonitrile and graphene oxide are used as preparation raw materials and a hydrolyzed polyacrylonitrile and graphene oxide compound fiber membrane is prepared through an electrostatic spinning technology and an alkali treatment method. Fibers of the fiber membrane have a lot of fusiform nodes and the nodes are induced through the graphene oxide. Furthermore, the fiber membrane is subjected to alkali treatment so that the hydrophility of the membrane is improved. Dual regulation and control on shape and surface hydrophility of the fiber membraneare carried out so that oil-water latex separation efficiency and anti-pollution performance of the membrane are remarkably improved. The preparation method of the separation membrane is simple and feasible and is safe and environmentally friendly; and the oil-water separation fiber membrane has very good application value and market prospect in the aspect of oil-containing wastewater treatment.

The invention relates to the field of plant protection and the insect raising technology, in particular to an orius sauteri raising method adopting drosophila carcinophila as feedstuff. The method changes the traditional method of adopting aphid or frankliniella occidentalis as feedstuff, the defect of the orius sauteri raising caused by the problems of the two kinds of feedstuff per se is changed, the characteristics of short growth period of the drosophila carcinophila and low production environment requirement are utilized, the feedstuff basis is provided for the large-scale raising of theorius sauteri, and in addition, the feeding effect is very excellent.

The invention discloses a preparation method for a high-refractive index hydrogenated silicon film, the high-refractive index hydrogenated silicon film, a light filtering lamination and a light filtering piece and relates to the technical field of optical films. The preparation method comprises the following steps that firstly, by means of magnetic controlled Si target sputtering, Si deposits on abase body, and a silicon film is formed; and secondly, the hydrogenated silicon film containing oxygen is formed through the silicon film under the environment where activated hydrogen and active oxygen are contained, the amount of the activated oxygen accounts for 4%-99% of the total amount of the activated hydrogen and the activated oxygen, or, the hydrogenated silicon film containing nitrogenis formed through the silicon film under the environment where the activated hydrogen and the activated nitrogen are contained, and the amount of the activated nitrogen accounts for 5%-20% of the total amount of the activated hydrogen and the activated nitrogen. According to the preparation method, sputtering and a reaction are separately conducted, firstly, by means of magnetic controlled Si target sputtering, Si deposits on the base body, then, plasmas of the activated hydrogen and the activated oxygen / nitrogen react with the silicon to obtain the oxygen or nitrogen containing SiH, the poisoning problem of a target is avoided, and the SiH film has a relatively high refractive index and relatively low absorption.

The invention relates to a low-carbon MgO-C brick containing modified phenolic resin and nanocarbon which are produced by nano carbon powder and a preparation method thereof. Phenolic resin bonding agent containing nano carbon powder is produced by ohenol, formaldehyde, a dispersant and nano carbon powder; the low-carbon MgO-C brick is produced by adopting fused magnesite, graphite, phenolic resin and an antioxidant as raw materials. The ultrasonic dispersion method adopted by the invention replaces partial graphite with the nano carbon powder and leads the nano carbon powder into the low-carbon MgO-C brick, thus obviously improving the anti-erosion ability and the oxidation resistance of the MgO-C brick. The brick has comparatively low thermal expansion coefficient, low thermal conductivity, low oxidative mass loss and thinner decarburized layer; a slag resistance experiment under conditions of 1600 DEG C, 3h heat preservation and carbon sequestration shows that erosion and osmosis phenomenon of the brick are not obvious, thereby being applicable to sites of converters, electric furnaces and ladle working linings, external scouring of furnaces, key components for continuous castings and refractories for blast furnace ironmaking etc., thus having comparatively large application range.

The invention relates to a hydraulic system of a hybrid power full-hydraulic excavator based on a CPR network, which belongs to the hydraulic system of excavators and solves the problems of low energy recovery and poor energy saving effect of the traditional fuel-electricity hybrid power system. A constant pressure variable output pump and a fixed displacement pump are connected with an output shaft of an engine and form a constant pressure fuel source together with a high pressure accumulator and a safety valve, and a central controller controls the engine to intermittently work in the best fuel saving region. Three double-way variable hydraulic pumps / motors and a hydraulic transformer are connected to the constant pressure fuel source and directly drive a working mechanism, and the central controller respectively controls the steering direction and the output volume of each hydraulic pump / motor and the transformer ratio of the hydraulic transformer through control assemblies. The system is suitable for the excavator; and only by retrofitting and transforming the traditional excavator, the fuel saving property of vehicles can be obviously increased, the emission of tail gases is reduced, the power performance of the vehicles is enhanced, and the service life of the engine and a brake device is prolonged.

The invention provides a compound feed for snakeheads, which relates to a feed for aquatic animals, in particular to a feed for fishes. The compound feed comprises the following raw materials in mass percent: 30-40% of steam fish meal, 0.2-0.3% of choline chloride, 20-30% of strong flour, 1-2% of calcium biphosphate, 15-25% of bean pulp, 0.1-0.2% of potassium chloride, 1.5-2% of squid extract, 0.1-0.2% of garlicin, 1-2% of spray-dried blood cells, 0.1-0.2% of glucan, 1-4% of soy protein concentrate, 0.5-1.0% of zeolite powder, 2-5% of fish soluble protein, 1-3% of wheat middling, 5-10% of peanut meal, 0.2-0.3% of compound vitamin, 3-5% of fish oil and 2-4% of compound minerals. Compared with the prior art, the product of the invention has the following advantages: (1), the feed has balanced nutrition to enable meet the requirements of snakeheads for nutrition in the growing process; (2), the feed has low coefficient, good palatability and high performance rice ratio; and (3), the feed has good stability in water and can not pollute the water quality of aquaculture water easily, and simultaneously, feed particles are prevented from sinking to cause that fishes can eat on the water surface.

The invention discloses a high-temperature glaze coating, a preparation method thereof, a binder and a using method of the coating, and aims at solving the problems of poor high-temperature resistance, low emissivity and absorptivity in a high-temperature zone, instability, and easiness of attenuation of the existing far-infrared energy-saving coating. The high-temperature glaze coating has the characteristics of good radiation heat transfer effect, high external blackness (the emissivity is greater than 0.95) and the like, and has the advantages of stable emissivity and absorptivity, non-attenuation performance and long service life in a long-term use process. Meanwhile, the coating can be tightly combined with a basal body by matching with the binder disclosed by the invention; the adhesive force is increased; a glazed ceramic polymer can be formed on the basal body after high-temperature sintering; the high-temperature glaze coating has the advantages of hardness, abrasive resistance, and long service life, and does not fall off; and the coating disclosed by the invention can also evenly coat the surface which cannot be covered by general coatings.

The invention discloses perfluoropolyether silane, a preparation method of the perfluoropolyether silane and application of the perfluoropolyether silane. The perfluoropolyether silane has a structural general formula shown as a formula I. Compared with a common perfluoropolyether material, the perfluoropolyether silane has the advantages that no trifluoromethyl branch chain exists; the perfluoropolyether material shown as the formula I provided by the invention can be more regularly arranged on the surface of a substrate, so that lower surface energy can be realized on the surface; further, the surface of the substrate has a better anti-fingerprint and easy-to-clean effect. Therefore compared with the prior art, the perfluoropolyether silane has better anti-fingerprint performance.

The invention relates to a fish and shrimp compound feed, in particular to a tilapia and litopenaeus vannamei polyculture compound feed, and belongs to the fields of compound feeds and processing thereof. The fish and shrimp polyculture compound feed is prepared from the following raw materials in percentage by mass: 7 to 12 percent of directly heated fish meal, 15 to 30 percent of soybean meal, 15 to 30 percent of rapeseed meal, 1 to 2 percent of liquid phospholipid, 5 to 15 percent of wheat short, 15 to 30 percent of corn, 8 to 20 percent of strong flour, 0.5 to 1 percent of calcium dihydrogen phosphate, 0.05 to 0.1 percent of compound vitamin, 0.5 to 1 percent of compound mineral, 0.02 to 0.3 percent of immune potentiator and 0.5 to 1 percent of fish oil. The fish and shrimp polyculture compound feed has the advantages of low feed coefficient, promotion of the fast growth of the tilapia and the litopenaeus vannamei, difficulty in polluting culture water quality, low cost and the like.

The present invention relates to safety monitoring system, and is flexibility monitoring method for large span bridge structure. Whole bridge pressure field is first established with water tanks set in the unchanged location, water pipes set horizontally and city water supply system to lead water pressure to deformation location of the bridge; and the pressure transducer is fixed to the measured part. The pressure change is converted into flexibility change and monitored with the computerized monitoring system, which consists of water tank, water pipes, pressure transducer, A / D converter, computer, etc. The present invention has high measurement stability, great measurement range, low measurement cost and high practical value.

The invention discloses an animal feed additive and a preparation method thereof. The animal feed additive is prepared from the following raw materials in parts by weight: 5-10 parts of corn fiber, 15-20 parts of soybean curb residue, 12-15 parts of zeolite powder, 40-60 parts of corn sugar residue, 1 part of compound biological active enzyme, 1 part of saccharomycetes, 1 part of lactobacillus, 1 part of bacillus and 1 part of bifidobacterium, wherein the compound biological active enzyme comprises the following components: 20,000 IU / g of xylanase, 10,000 IU / g of pectinase, 400 IU / g of cellulose and 200 IU / g of protease. The preparation method comprises the steps of raw material weighing, pre-processing, mixing, dual fermentation and packaging. The animal feed additive disclosed by the invention can be used for improving the digestibility to promote the growth and development of animals, regulating the gastrointestinal micro-ecological balance to enhance animal immunity and improve disease resistance, improving animal feed intake, improving the cultivation environment to reduce respiratory diseases, and improving the quality and quantity of cultivated products.

A manipulator includes a controlling seat, a robot arm assembly and a polishing mechanism. The robot arm assembly is rotatably connected to the controlling seat; and the polishing mechanism is mounted on the robot arm assembly. The polishing mechanism includes a housing with a first mounting portion and a second mounting portion, a pair of magnetic valves mounted in the housing and electrically connected to the controlling seat, a first polishing assembly partially received in the first mounting portion, and a second polishing assembly partially received in the second mounting portion. The first polishing assembly and the second polishing assembly define an angle therebetween and are respectively connected to the pair of magnetic valves, such that the pair of magnetic valves controls the first and the second polishing assembly respectively to polish one or more workpiece. The present invention further discloses a polishing mechanism of the manipulator.

The invention provides a hematoxylin eosin staining solution, comprising a hematoxylin staining solution and an eosin staining solution, wherein the hematoxylin staining solution comprises hematoxylin, an alumium salt, an oxidizing agent, benzalkonium chloride, an alcohol, a weak acid and water; the eosin staining solution comprises eosin, Biebrich scarlet, flame red B, water and ethanol. The invention also provides a preparation method of the hematoxylin eosin staining solution, and a dyeing method thereof. When the hematoxylin eosin staining solution provided by the invention is used for dyeing slices, the effects of clear background, distinct gradation, good dyeing effect, easy slice reading, not easy fading and convenience for long-term storage can be achieved.

The invention relates to an ellipsoidal high-power microwave plasma diamond film deposition device which is composed of a staisecase annular microwave coupled system, an annular quartz microwave window arranged at the staircase of an annular antenna, an ellipsoidal microwave resonant cavity, an adjustable deposition table, a conical upper reflecting body, an adjustable cylindrical lower reflecting body, an inlet and outlet, a temperature measurement hole, a view window and the like. By utilizing the design of the ellipsoidal upper and lower focal points, the conical upper microwave reflecting body is positioned on the upper focal point, the deposition table is positioned on the lower focal point, and the electric field is distributed centrally, so the excited plasma has high position stability and high density; the hidden microwave window can be protected from plasma heating, pollution and etching; the adjustable microwave lower reflecting body and the deposition table optimize the plasma distribution in real time; the inner wall of the ellipsoidal resonant cavity is far from the high-temperature plasma region, so that the heat radiation of the plasma to the inner wall of the cavity is reduced, thereby avoiding depositing foreign matters; and all the components of the device are cooled with water. The device can implement high-efficiency deposition of a large-area high-quality diamond film under the condition of high power.

The invention relates to the field of graphene materials, particularly relates to preparation methods for graphene micro-sheets and particularly relates to a method for preparing the graphene micro-sheets by using a counter-jet jet mill. According to the method, the graphene sheets are obtained through enabling melted ferric chloride and potassium chloride to enter an interlayer of graphite, enabling the powder materials to be in collision through high-speed airflow in the counter-jet jet mill by using the characteristic of brittleness of ferric chloride and potassium chloride crystal grains and the characteristics of good fluidity and difficulty in agglomeration of talcum powder, delaminating the graphite and the talcum powder by generated impact force, shearing force and frictional force, carrying out further separation by a grading room, refluxing unqualified powder material to a crushing chamber, and yielding delaminated graphite and talcum powder as well as gas together. The continuous and large-scale production of the graphene micro-sheets, which are uniform in layer number dispersion and good in fluidity and are not prone to agglomeration, is achieved, the yield is high, the cost is low, no pollution is caused, and the layer thickness meets the requirements of use in the fields of rubber reinforcing, plastic reinforcing, coating material anticorrosion, lubrication and sewage treatment, so that the promotion of the large-scale application of graphene is facilitated.

The invention provides an oil and water separator based on a micron-nano hiberarchy net membrane and a preparation and application method thereof. The oil and water separator comprises an oil and water separation device with a micron-nano hiberarchy net membrane, a constant water surface device, a drainage device and a water collection device, wherein the oil and water separation device with a micron-nano hiberarchy net membrane comprises an oil and water mixture liquid collection device and the micron-nano hiberarchy net membrane; the micron-nano hiberarchy net membrane comprises hole-shapedstructure fabric net matrix material with the micron structure and a nanorod array structure material layer vertical to the surface of the material; and the nanorod array structure material layer vertically grows on the surface of the hole-shaped structure fabric net matrix material with the micron structure. The oil and water separator has the characteristics of super hydrophile and underwater super oleophobic performance. The oil and water separator based on a micron-nano hiberarchy net, which is provided by the invention, is stable, efficient and environmentally friendly and the like. Whenthe oil and water separator is used for separating oil and water, water can permeate through the net membrane but oil can not permeate through the net membrane, and therefore the oil and water separator can be reused several times.

The invention provides a hybrid power full hydraulic loading machine hydraulic system based on a pressure common rail system. The system is used for solving the problems that an existing hybrid power system is low in transmission efficiency, low in energy recycling rate and low in reusing rate. In the process of braking, a walking hydraulic pump or a motor works in a pump operating condition, braking kinetic energy of a loading machine is recycled and stored in a high-pressure hydraulic accumulator. In a working condition of descending of a movable arm cylinder, the pressure is changed through a hydraulic transformer, and hydraulic energy is stored in the high-pressure hydraulic accumulator. In the process of starting of a vehicle and lifting of the movable arm cylinder, the recycled hydraulic energy can supply power for the vehicle. An active stamping function of the hydraulic pump plays the role of adjusting operating conditions of an engine so that the engine works in the optimum economic zone, and meanwhile the defect that the high-pressure hydraulic accumulator is small in energy density is recovered. The hybrid power full hydraulic loading machine hydraulic system has the advantages of being low in complexity, good in sealing performance, compact in structure, light, low in manufacturing cost and not prone to be polluted.

An electronic cigarette comprises a first shell and a second shell, wherein a cigarette cartridge tube and an atomizer are contained in the first shell, the cigarette cartridge tube comprises a cigarette cartridge tube carrier used for storing cigarette liquid and a sealing part which is sealed at the end, facing the atomizer, of the cigarette cartridge tube carrier, the atomizer comprises a heating element, a puncture part and an atomizer fixing seat used for bearing the heating element and the puncture part, the second shell is connected with the first shell in a screwed mode, a pushing ejecting portion is fixed in the second shell, the pushing ejecting portion can push the atomizer fixing seat when the first shell is connected with the second shell in the screwed mode to enable the puncture part to puncture the sealing part of the cigarette cartridge tube, and therefore the cigarette liquid is educed. As the cigarette liquid is fed into the cigarette cartridge tube during production and sealed through the sealing part, the cigarette liquid can enter the heating element only by puncturing the sealing part through the puncture tube in the use process, the problem that traditional storing or placing of the cigarette liquid is inappropriate does not exist, and the cigarette liquid is not prone to being polluted.

The present invention is one kind of green compounded and puffed feed for rock fish. The green compounded and puffed feed is prepared with steamed fish meal 35-50%, soybean residue 15-20%, pork bone powder 5-12%, beer yeast 3-5%, cuttle fish paste 2-6%, fish oil 1.0-2%, vegetable oil 1-2%, macaroni flour 10-20%, shrimp shell powder 3-6%, lecithin 1-2%, vitamins 0.3-0.5%, compounded mineral 0.5-1% and choline chloride 0.3-0.5%, and through mixing, crushing, extruding to pelletize, and stoving. The green compounded and puffed feed has simple compounding process and balanced nutrients, can meet the requirement of the growth of rock fish, and results in less water contamination.

This invention relates to a real time fluorescence quantitative PCR test method for H5, H7 and H9 sub-avian flu viruses including the following steps: set-up of standard molecules, the design of specific virus quantitative PCR primer and the probes, pick-up of virus sample RNA, the inverse transcription polyenzyme chain reaction to the pic-up sample RNA to get a sample cDNA, the optimization and set-up of the PCR quantitative test method for M genes.

The invention discloses a waterborne two-component light-color in-tank electrostatic conduction anticorrosive paint, which is prepared from the following components by weight percent: 8-20% of running water, 30-45% of waterborne epoxy emulsion, 7-15% of waterborne epoxy curing agent, 8-20% of anti-rust pigment, 1-7% of white pigment, 1-7% of white filler, 8-18% of light color conductive filler, 0.1-0.3% of anti-settling agent, 0.1-0.4% of wetting dispersant A, 0.1-0.3% of wetting dispersant B, 0.8-2% of coalescing agents, 0.2-0.6% of defoamer, 0.1-0.3% of flatting agent, 0.1-0.6% of antirust agent, and 0.5-2% of thickener. The two-component waterborne epoxy resin and waterborne curing agent are taken as main film-forming matters, so that the condition that the coating has high crosslinking density and barrier property is ensured by chemical crosslinking reaction of two components.

The invention relates to a surface treatment process of wall decorative board, which takes one of the fiber-reinforced calcium silicate board, fiber cement board, fiberglass magnesium oxide board or the like as a substrate, firstly coats an UV coating on the surface of the substrate, and performs dust exhaustion and sanding to the cured surface; re-coating PU or UV white bottom; and then performs dust exhaustion and sanding to the surface; and then coats PU or UV glue again on the surface, uses low-temperature heat-transferring technology to transfer the pattern on the heat-transferring die to the surface; finally, coats a UV protective coating on the surface of the transferred pattern. This wall decorative board has high-grade, wear-resistance, anti-aging, non-susceptibility to contamination, good fire prevention and waterproof performance.

The invention relates to a thermal insulation compound wall body of a dry hanging keel decoration plate and a construction method, wherein a prefabricated thermal insulation keel is mounted on a basal layer wall body through a mechanical anchor firmware, and then a negative angle column and a positive angle column are mounted, heat-insulating material is filled between the positive angle column and the negative angle column, both lateral sides of the invention are respectively equipped with two thermal insulation keels, and J-shaped slotted plates are respectively mounted on the two thermal insulation keels, and a prefabricated door-window insulating sleeve hole is mounted on a door-window opening, the periphery of a sash is filled and infused with foaming polyurethanes, a heat-insulating board is filled between the thermal insulation keels, and the thermal insulation keels are fixed by an anchor firmware with a round plate, and the foaming polyurethanes are infused in gaps, at last a cladding decoration panel is fixedly mounted on the outer side of the thermal insulation keels by self-threading screws. The thermal insulation property of the thermal insulation compound wall body is excellent, and the thermal insulation compound wall body achieves an integral dry construction, the architectural exterior facing is rich and colorful, the cubic effect is strong, the invention is not easy to be polluted, which can be widely applied to civilian constructions which are newly constructed, enlarged, and rebuilt and needs thermal insulation and heat insulation, in particular to villas whose heights of eaves are less than 20m and floors are less than 3 to 4, and outer-eaves decoration of middle grade and high grade constructions.

The invention provides an automatic pointing glue injection machine of single-component glue. The automatic pointing glue injection machine comprises a base, a guide driving wheel and a guide pulley, a vibration-absorption anti-sliding rail, a variable frequency motor, a pneumatic piston cylinder and a glue charging barrel, wherein the guide driving wheel and the guide pulley are mounted under the base, the vibration-absorption anti-sliding rail is used for bearing the guide driving wheel and the guide pulley, the variable frequency motor is mounted on the base and is used for driving the guide driving wheel and the guide pulley, the pneumatic piston cylinder is mounted on the base, the glue charging barrel protective jacket is located in front of the pneumatic piston cylinder, and the glue charging barrel is arranged in the glue charging barrel protective jacket. The glue injection machine is simple in structure and convenient to operate; the machine is driven by the variable frequency motor on the vibration-absorption rail to linearly move front and back; an air cylinder is used for pushing and extruding glue so that the single-component glue in the cylinder is used for automatically carrying out pointing glue injection work on gaps; the widths of a pointing glue injection strip straight line and a pointing glue strip are uniform; the disadvantages that manual pointing glue injection is zigzag and the width is not equal are overcome; and the pointing labor intensity of constructors is greatly alleviated, and the construction efficiency and the construction quality are improved.

The invention discloses a detection kit for quickly identifying donkey skin, horse skin and mule skin, which is used for accurately identifying donkeys, horses, mules and jennets by creatively applying 16SrRNA genes of CKM (creatine kinase) karyogene (nDNA) and mitochondrial genome DNA (mtDNA) at the same time from genetic background of the horses, the donkeys and the mules. The kit adopts an molecular beacon probe (MB) method to carry out a quintuple multicolor fluorescence quantitative PCR (polymerase chain reaction) detection technology, and also can detect donkey, horse, mule and jennet components; and moreover, the kit is added with exogenous internal reference as an interior label, can detect and avoid false negative results produced by PCR inhibitor contained in a sample. The quintuple multicolor fluorescence quantitative PCR is used for detecting in the same tube without opening a cover, so that the detection kit is not easy to pollute, and is accurate and stable, simple to operate, extremely high in sensitivity, strong in specificity, and the like, and therefore, a novel way is explored for identifying the donkey skin, horse skin and mule skin.

The invention relates to a bacillus subtillis strain containing ramie degumming activity, preparation and application thereof, which uses a system taking the strain as the core for ramie degumming. The strain of the system has high reproductive rate, high yield of pectase and xylanase, short production period, strong contamination resistance and favorable heat-resistant quality. The system and thecultivating process are safe to operate, have no toxicity and pollution no environment. Compared with the prior art, the invention has simple technology, is suitable for large-scale industrial production and the like. When the system is used for ramie degumming, degumming time is short, the dispersion coefficient of ramie fiber can reach 100%, degumming ratio can reach above 90%, and the qualityof refined dry ramie can reach the standard of chemical degumming.