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Bacillus subtillis strain containing ramie degumming activity, preparation and application thereof

A technology of Bacillus subtilis and ramie, which is applied to Bacillus subtilis strains and its application fields, can solve the problems of unsatisfactory degumming effect, easily damaged fiber strength, long degumming cycle, etc., and is suitable for large-scale industrial production without environment Contamination, short degumming time effect

Inactive Publication Date: 2010-02-24
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are also reports abroad on the use of filamentous fungi such as Aspergillus niger for hemp degumming. However, fungal degumming is common: slow growth, long degumming cycle, acidic growth conditions, fungi can produce cellulase, fiber strength is easily damaged, and the residual glue rate High, the degumming effect is not very ideal

Method used

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  • Bacillus subtillis strain containing ramie degumming activity, preparation and application thereof
  • Bacillus subtillis strain containing ramie degumming activity, preparation and application thereof
  • Bacillus subtillis strain containing ramie degumming activity, preparation and application thereof

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Effect test

Embodiment 1

[0044] Screening and Identification of Degumming Dominant Strains

[0045] 1) Cut the original diameter of rotten ramie into small pieces of 1.5-2cm, mix well and take 5g into a 250mL Erlenmeyer flask filled with 200mL enrichment medium, (enrichment medium 1: beef extract 0.3g peptone 1g NaCl 0.5g water 1000ml, pH7.4-7.6; enrichment culture 2: K 2 HPO 4 0.15g, ferric ammonium citrate 0.15g, MgSO 4 0.15g, glycerin 6g, citric acid 0.6g, L-glutamic acid 1.2g, water 1000ml, pH 7.4) after one month of static enrichment culture at room temperature, dilute 10 -3 , 10 -4 , 10 -5 , 10 -6 After the gradient, spread on ramie separation medium (ramie 15g, (NH4) 2 SO 4 5g,K 2 HPO 4 1g, MgSO 4 0.5g, KCl 0.5g, FeSO 4 0.01, 20g agar, 1000mL water, natural pH, about 7.0-7.2) at 28°C, cultured for 5 days, picked a single colony and streaked it on the ramie medium. After the colony is formed, observe and record the morphological characteristics of the colony such as color, surf...

example 2

[0050] Ramie degumming process and its result

[0051] Microbial degumming of ramie was carried out in 250ml Erlenmeyer flasks, the liquid volume in each Erlenmeyer flask was 100ml (containing 5g of ramie raw hemp), the bath ratio was 1:20, after heating and boiling for 30min, adjust the pH value, and after sterilizing at 121°C for 20min , The flask was cultured on a shaker under different conditions. Until the degumming is finished, remove the degumming solution, boil in a boiling water bath for 5 minutes, rinse with tap water several times to remove the bacteria on the ramie, and stop the degumming.

[0052] The bacterial strain was inoculated in the ramie fermentation medium containing sterilized treatment (ramie raw hemp 5%, (NH 4 ) 2 SO 4 0.5%, MgSO 4 0.05%, KCl 0.05%, K 2 HPO 4 0.1%, FeSO 4 0.001%), 40°C, 200rpm for shaker fermentation culture, after 1 day, it can be seen that the ramie fibers are obviously dispersed, and the color of the fermented liquid beco...

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Abstract

The invention relates to a bacillus subtillis strain containing ramie degumming activity, preparation and application thereof, which uses a system taking the strain as the core for ramie degumming. The strain of the system has high reproductive rate, high yield of pectase and xylanase, short production period, strong contamination resistance and favorable heat-resistant quality. The system and thecultivating process are safe to operate, have no toxicity and pollution no environment. Compared with the prior art, the invention has simple technology, is suitable for large-scale industrial production and the like. When the system is used for ramie degumming, degumming time is short, the dispersion coefficient of ramie fiber can reach 100%, degumming ratio can reach above 90%, and the qualityof refined dry ramie can reach the standard of chemical degumming.

Description

technical field [0001] The invention belongs to the field of bacillus subtilis strains and applications thereof, in particular to a bacillus subtilis strain with ramie degumming activity, its preparation and application. Background technique [0002] The scientific name of Ramie (Ramie) is Boehmeria nivea (Linn.) Gaudich, which is a perennial herbaceous plant belonging to the family Urticaceae. The dried raw hemp contains 60-70% cellulose and 30-35% non-cellulose colloid components. These polymer colloid compounds are mainly: pectin, hemicellulose, lignin and a small amount of fatty wax and ash. As a raw material for textile fabrics, ramie fiber not only has good heat absorption, moisture dissipation, breathability and other incomparable advantages, but also has disadvantages such as poor elasticity, unsatisfactory wrinkle resistance and abrasion resistance, and itchy feeling when wearing. In order to meet people's extensive needs of returning to nature and pursuing greenn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20D01C1/04C12S3/04C12Q1/34G01N21/31C12R1/125
Inventor 张兴群陈杨栋曹张军
Owner DONGHUA UNIV
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