14 results about "Creatine kinase" patented technology

The invention discloses combined probiotics for improving sarcopenia as well as a preparation method and application of the combined probiotics, and belongs to the field of probiotics. The invention relates to a combined probiotic for improving sarcopenia. The combined probiotic comprises a lactobacillus reuteri body, a lactobacillus johnsonii body, a lactobacillus fermentum body and a bifidobacterium adolescentis body. The combined probiotics disclosed by the invention can be used for remarkably improving the overall symptoms of sarcopenia model mice, including inhibiting weight loss, constipation symptoms and colonic mucosa destruction, prolonging load swimming time, improving muscle endurance, improving skeletal muscle atrophy, improving the expression of I-type slow muscle fiber protein MyHC I and promoting slow muscle fiber repair, and has the effects of preventing and treating sarcopenia. The invention discloses a probiotic intervention method for treating sarcopenia, which comprises the following steps of: preparing probiotics for treating sarcopenia, reducing the content of inflammatory cytokines in muscular tissues, increasing the content of hepatic glycogen, improving systematic metabolism of mice with sarcopenia and damaged serum markers, including lactic acid, lactic dehydrogenase, creatine kinase, creatinine, alanine transaminase ALT and aspartate transaminase AST, providing a new scheme for probiotic intervention for sarcopenia, and being used for preparing related functional products or intervention means.

The application discloses an evaluation method for predicting the safety of EGFR TKIs monotherapy for NSCLC. The method comprises the following steps: obtaining the data of a plurality of patients receiving EGFR TKIs treatment; taking MDRAE (maximum grade of drug-related adverse event) as a safety index, constructing a safety evaluation final model, and the final model is an ordered logistic regression model, the covariates of the final model include normalized steady-state trough concentration, EGFR TKIs treatment history, gender, baseline glutamyltransferase, baseline uric acid, baseline creatine kinase, baseline platelets and baseline lymphocytes; obtaining the data of a target patient, inputting the numerical values of each covariate in the data of the target patient into the final model; determining the first probability of MDRAE of the target patient being grade 3 and above, the second probability of MDRAE being grade 2 and the third probability of MDRAE being grade 1 and below according to the final model, and taking the grade corresponding to the maximum probability in the three probabilities as the predicted grade of MDRAE of the target patient. The application improves the accuracy of predicting the safety of EGFR TKIs monotherapy for NSCLC.

The invention discloses application of hydrogen-rich water in preparation of a drug for treating Duchenne type muscular dystrophy, and particularly relates to the technical field of medicines.The random control experiment is adopted, homologous c57 mice serve as a normal control group, mdx mice are randomly divided into a model group and a hydrogen-rich water group, and the exercise ability is evaluated through a four-limb holding power test and a rotating bar test; detecting mouse creatine kinase by an enzyme-linked immunosorbent assay; carrying out hematoxylin-eosin, NADH-tetrazole reductase and improved Goori three-color dyeing, and carrying out cytochrome C oxidase, succinate dehydrogenase and SDH-COX combined dyeing to observe the pathological change of the skeletal muscle tissue; the invention discloses that the muscle injury condition of a Duchenne type muscular dystrophy model mouse is improved by the hydrogen-rich water through a mitochondrial protection mechanism, and the hydrogen-rich water has a relatively high application prospect and a relatively high economic value.

The invention belongs to the technical field of protein peptide preparation, and particularly relates to wheat oligopeptide with an anti-fatigue effect and a preparation method of the wheat oligopeptide. According to the method, the raw materials are subjected to enzymolysis by using the first compound enzyme containing bacillus subtilis protease and bromelain and the second compound enzyme containing trypsin, flavourzyme and papain step by step, and treatment of centrifugation, ultrafiltration and the like is combined, so that the yield and the taste of the product are improved, and the proportion of small molecular weight peptide in the product is high. Therefore, the wheat oligopeptide prepared by the method is high in yield and good in taste, not only is beneficial to improving the endurance of the body and prolonging the exercise time, but also can improve the energy metabolism condition in the body, delay the accumulation of lactic acid, urea nitrogen and creatine kinase in the body after exercise and increase the glycogen storage amount, and macroscopically shows certain anti-fatigue ability. The technical scheme of the invention has strong practicability, is beneficial to industrial production, and can be applied to preparation of anti-fatigue functional food.

The invention relates to application of a Fe < 3 + >-ATP-CK cascade regulation type carbon dot fluorescent probe in dynamic monitoring of creatine kinase content, and relates to the field of new application of carbon dots. Fluorescence of B, N-CDs is quenched by Fe < 3 + >, and then fluorescence is recovered after ATP is added through specific binding of Fe < 3 + > and ATP. When CK catalyzes ATP to react with Cr, fluorescence is quenched again due to reduction of the ATP content. On the basis of high-selectivity competitive combination of Fe < 3 + > and ATP, interference of ADP, AMP and other analogues can be eliminated. The multiple verification mechanisms of Fe < 3 + > quenching, ATP recovery and CK re-quenching significantly reduce the false positive result, and enhance the detection reliability. A portable sensing platform constructed based on a smart phone establishes a new method for bedside detection of CK, establishes a brand new strategy for CK detection, and shows a wide prospect in rapid screening application of clinical diagnosis of myocardial infarction and the like.

The traditional Chinese medicine composition is characterized by being prepared from the following traditional Chinese medicinal materials in parts by weight: 3 to 9 parts of rhizoma chuanxiong, 3 to 7 parts of rhizoma atractylodis, 6 to 14 parts of rhizoma cyperi, 6 to 14 parts of medicated leaven, 3 to 7 parts of euphorbia helioscopia, 10 to 20 parts of poria cocos, 8 to 12 parts of fructus trichosanthis, 8 to 12 parts of radix salviae miltiorrhizae, 8 to 12 parts of herba epimedii, 3 to 9 parts of pepper, 8 to 12 parts of herba patriniae and 8 to 12 parts of caulis spatholobi. After the traditional Chinese medicine composition is used for treating acute myocardial infarction, a remarkable effect is achieved on PCI postoperative improvement of acute myocardial infarction, troponin and creatine kinase isoenzyme indexes are reduced, and the left ventricular ejection fraction is in a normal range after cardiac color ultrasound display treatment. Through comprehensive conditioning, symptoms such as chest distress, chest pain, shortness of breath and hypodynamia of patients are improved, cardiac function recovery is promoted, complications such as restenosis and heart failure are prevented, and life quality is improved.

The invention relates to the technical field of traditional Chinese medicine agentia, and particularly discloses a traditional Chinese medicine compound anti-fatigue agentia based on American ginseng and a preparation method, the traditional Chinese medicine compound anti-fatigue agentia comprises the following components in parts by mass: 5-10 parts of American ginseng, 10-20 parts of pericarpium citri reticulatae, 10-20 parts of fructus lycii, 4-8 parts of schisandra chinensis, 8-16 parts of Chinese yam, 4-8 parts of bighead atractylodes rhizome, 5-10 parts of rhizoma alismatis, 10-20 parts of radix ophiopogonis, 0.5-1.5 parts of parasitic loranthus, 2-6 parts of radix aconiti carmichaeli, 5-8 parts of fresh ginger, 3-9 parts of lucid ganoderma and 4 According to the traditional Chinese medicine composition, the content of glycogen in a human body is increased, the content of lactic acid, urea nitrogen and creatine kinase in the human body is reduced, a good anti-fatigue effect is achieved, an extracting solution prepared from traditional Chinese medicine components has a green and healthy treatment effect on the human body, and impurities which are useless and harmful to the human body can be removed in a distillation and purification mode.

This invention provides a method for site-specific replacement of the creatine kinase promoter in the Huaxi bovine gene promoter, belonging to the field of animal gene editing and genetic breeding technology. It also provides a method for enhancing the function of bovine muscle cells. FTO Gene expression reagents include crRNA for upstream site knockout, crRNA for downstream site knockout, a Cas12i nuclease expression vector, and an FTO homologous substitution vector; the FTO homologous substitution vector includes... FTO upstream homologous arm of gene promoter, MCK gene promoter and FTO Downstream homologous arm of the gene promoter. The reagent was used in West China bovine cells. FTO Application in gene promoter replacement. This invention can specifically improve the expression efficiency of genes related to fat development in muscle tissue, providing an efficient and precise technical means for the genetic improvement of the performance of beef from Western China.

The invention discloses a lactobacillus rhamnosus CQFP202457 and application of the lactobacillus rhamnosus CQFP202457 in relieving exercise-induced fatigue, and belongs to the field of microorganisms. The invention provides a lactobacillus rhamnosus CQFP202457, and the preservation number of the lactobacillus rhamnosus CQFP202457 is CGMCC (China General Microbiological Culture Collection Center) No.29784. The lactobacillus rhamnosus CQFP202457 can be used for preparing the lactobacillus rhamnosus CQFP202457. The invention also provides application of the strain in preparation of a composition for relieving exercise-induced fatigue. Experimental results show that the lactobacillus rhamnosus CQFP202457 can significantly prolong exhaustion exercise time, improve exercise tolerance, reduce levels of lactic acid, urea nitrogen and creatine kinase in serum after exercise, reduce accumulation of exercise metabolites and relieve muscle injury. The invention provides a new microbial resource for developing a product for relieving exercise-induced fatigue.

This invention belongs to the field of biomedical technology, specifically relating to a composition for treating ischemic heart disease and its application. This invention combines two or more of ginsenoside Rg1, ginsenoside Rb1, ginsenoside Re, and notoginsenoside R1, which can significantly downregulate the levels of myocardial enzymes, aspartate aminotransferase (AST), creatine kinase (CK), and lactate dehydrogenase (LDH) in myocardial coronary effusion, with an inhibitory effect significantly higher than that of a single saponin component and the positive control drug verapamil. Simultaneously, the composition exhibits better recovery capabilities for coronary flow (CF), left ventricular developmental pressure (LVDP), maximum rate of decrease in left ventricular pressure (-dp / dtmax), and heart rate (HR) in myocardial coronary effusion. Therefore, the composition can alleviate and treat myocardial ischemia-reperfusion injury through multiple targets and mechanisms, fundamentally inhibiting the development of ischemic heart disease.

The invention belongs to the technical field of bioengineering, and particularly relates to a prokaryotic cell-free translation system buffer solution and application thereof. The prokaryotic cell-free translation system buffer solution comprises HEPES-KOH (pH 8.0), magnesium glutamate, ATP (adenosine triphosphate), GTP (glutathione triphosphate), potassium glutamate, creatine kinase and creatine phosphate. According to the present invention, the research accidentally discovers that the translation efficiency of the prokaryotic acellular translation system buffer solution constructed after the reduction of 28 unnecessary components is substantially the same as the translation efficiency of the original report, and the translation efficiency of the prokaryotic acellular translation system is significantly improved after the concentration is optimized, such that the good practical application value and the good prospect are provided.

The invention relates to the technical field of bioengineering, in particular to a preparation method of stable natural creatine kinase. Comprising the following steps: removing fascia fat from a pig heart, adding a DK1 buffer solution, and crushing by a meat grinder to obtain an enzyme extracting solution A; adding ammonium sulfate into the crude enzyme extract A for multiple times, adjusting the pH value by using DK2, centrifuging, and taking supernatant to obtain an enzyme extract B; adding solid ammonium sulfate into the enzyme extracting solution B to carry out salting-out precipitation, centrifuging to take precipitate, and dialyzing with DK2 to obtain an enzyme extracting solution C; purifying through an ion exchange column, and sequentially eluting through a DK3 buffer solution to obtain a creatine kinase solution; carrying out concentration dialysis liquid change treatment on creatine kinase by using DK4 to obtain an enzyme extracting solution D; adding a liquid protective agent into the enzyme extracting solution, and carrying out stabilizer research; through the mode, high-activity and high-stability creatine kinase can be obtained, the process repeatability is high, the cost is low, and large-scale production of the process is facilitated.

The present disclosure relates to compounds that are capable of inhibiting creatine kinase. The present disclosure also relates to methods of treating cancer, such as hematological malignancies.

The invention provides a method for site-specific replacement of a West China cattle gene promoter with a creatine kinase promoter, and belongs to the technical field of animal gene editing and genetic breeding. The invention discloses a reagent for improving FTO gene expression in bovine muscle cells. The reagent comprises crRNA for upstream site knockout, crRNA for downstream site knockout, a Cas12i nuclease expression vector and an FTO homologous replacement vector, the FTO homologous replacement vector comprises an FTO gene promoter upstream homologous arm, an MCK gene promoter and an FTO gene promoter downstream homologous arm. The reagent is applied to FTO gene promoter replacement in western China cattle cells. According to the method, the expression efficiency of fat development related genes in muscle tissues can be specifically improved, and an efficient and accurate technical means is provided for genetic improvement of the performance of the beef in the western China.