CTnI, Myo and CK-MB three-in-one colloidal gold test strip, kit thereof, and making methods of test strip and kit

A technology of CK-MB and detection test paper, which is applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of doctors' misjudgment or missed judgment, large detection error, etc., and achieve simple detection, easy popularization, and simplified operation process Effect

Inactive Publication Date: 2013-08-28
JIANGSU KEYGEN BIOTECH CORP LTD
View PDF11 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, most of them only detect a single index of myocardial infarction, the detection error is large, and it is easy for doc...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • CTnI, Myo and CK-MB three-in-one colloidal gold test strip, kit thereof, and making methods of test strip and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Composition of Troponin I (cTnI), Myoglobin (Myo), Creatine Kinase Isoenzyme (CK-MB) three-in-one rapid detection test strip (colloidal gold method)

[0030] 1. Sample processing bottle: The sample processing bottle (purchased from Hangzhou Beiyu) is filled with 1mL sample processing solution (phosphate buffer solution, the main raw material is purchased from Sinopharm Group).

[0031] 2. Test strip: 60*5mm bottom plate (purchased from Shanghai Jieyi Company); 15*5mm thick fiber filter paper for absorbent pad (purchased from Shanghai Jieyi Company); 25*5mm nitrocellulose membrane (purchased from Millipore Company ) were sequentially coated with cardiac troponin I antibody (purchased from abcam company), myoglobin antibody (purchased from abcam company), creatine kinase isoenzyme antibody (purchased from abcam company), goat anti-mouse IgG antibody (purchased from abcam company) abcam Company); the sample pad (purchased from Shanghai Jieyi Company) was attache...

Embodiment 2

[0032] Embodiment 2: the preparation of kit

[0033] 1. Preparation of sample processing solution: Prepare sample processing solution: prepare phosphate buffer solution (pH7.0±0.2), dispense it into sample processing bottles, 1mL / bottle.

[0034]2. Preparation of test strips:

[0035] 2.1. Dilute human cTnI monoclonal antibody, human Myo monoclonal antibody, human CK-MB monoclonal antibody, and goat anti-mouse IgG antibody to a coating concentration of 1.0-1.2 mg / mL, and divide the four coating solutions into nitric acid respectively. At the designated position on the cellulose membrane, dry the coated nitrocellulose membrane and store it under the drying conditions of 20-30°C and relative humidity of 30-35% for 5 hours.

[0036] 2.2. Preparation of gold label pad: Colloidal gold was prepared by trisodium citrate reduction method, and a certain proportion of monoclonal antibody cTnI / Myo / CK-MB was slowly added to the gold sol, so that colloidal gold and antibody formed monoclo...

Embodiment 3

[0041] Embodiment 3 detection method

[0042] 3.1. Specimen collection

[0043] 1. Collection of whole blood specimens: Fingertip, earlobe end or vein specimens should be used as soon as possible after collection, and anticoagulant blood should be used within 24 hours to avoid blood fusion.

[0044] 2. Collection of serum / plasma specimens: Serum / plasma specimens are obtained by centrifugation after venous blood collection. Specimens can be stored at 2-8°C for one week. Long-term storage needs to be frozen, but repeated freezing and thawing should be avoided.

[0045] 3. Take 100-200ul of whole blood, serum, and plasma samples and drop them into the sample loading window. If the whole blood is relatively viscous, add 1-2 drops of unused sample processing solution to the sample loading window.

[0046] 4. The detection temperature is 15-30°C and the humidity is 40-60% is the best.

[0047] 5. Interpret the result after 10-15 minutes:

[0048] 5.1. If the sample contains myogl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a cardiac troponin (cTnI), myoglobin (Myo) and creatine kinase-MB (CK-MB) three-in-one colloidal gold test strip, a kit thereof and making methods of the test strip and the kit. The test strip and the kit are made through adopting a chromatographic technique and a double antibody sandwich principle, and the human cTnI, Myo and CK-MB levels in a clinic specimen (whole blood/serum/blood plasma) can be detected through one-time operation, so the operation process is simplified.

Description

technical field [0001] The invention belongs to the technical field of immunodiagnostic reagents, and relates to a qualitative detection of human cardiac troponin I, myoglobin and myoglobin in clinical samples (whole blood / serum / plasma) by using colloidal gold immunochromatography technology and the principle of double antibody sandwich method. A test strip, kit and preparation method for acid kinase isoenzymes. Background technique [0002] Myocardial infarction is the most dangerous type of coronary heart disease. With the improvement of living standards, its incidence rate has been increasing year by year. It has now posed a major threat to human health and survival and is a social problem faced by countries all over the world. According to statistics, in the past, two-thirds of patients with acute myocardial infarction died before being sent to the hospital, and among the patients who were sent to the hospital, the mortality rate was still as high as 30%. [0003] From ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/68G01N33/577G01N33/573
Inventor 不公告发明人
Owner JIANGSU KEYGEN BIOTECH CORP LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap