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Reagent for detecting myocardial infarction and application of reagent

A technique for myocardial infarction and reagents, applied in the field of disease diagnosis, can solve the problems of limitation and atypical early clinical symptoms of AMI patients

Inactive Publication Date: 2017-09-15
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, current diagnosis is based on clinical symptoms, ECG abnormalities and biochemical indicators, but about 1 / 3 of AMI patients have atypical early clinical symptoms, and about 1 / 2 of patients cannot have characteristic ST-segment changes in ECG. Among the commonly used biochemical indicators Such as creatine kinase, lactate dehydrogenase, myoglobin, etc., are subject to some limitations in clinical application due to their specificity or sensitivity

Method used

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  • Reagent for detecting myocardial infarction and application of reagent
  • Reagent for detecting myocardial infarction and application of reagent
  • Reagent for detecting myocardial infarction and application of reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The collection of embodiment 1 sample and total RNA extraction

[0047] The peripheral blood of patients with myocardial infarction and 6 cases of healthy controls were collected in the hospital from March 2015 to September 2016. Diagnostic criteria for acute myocardial infarction: developed according to the diagnostic criteria recommended by the third "Global Unified Definition of Myocardial Infarction" in 2012. An increase and / or decrease in the level of myocardial necrosis markers (mainly troponin) was detected, exceeding the 99th percentile value of the upper limit of the reference value at least once, and accompanied by at least one of the following ischemic symptoms.

[0048] 1) Symptoms of myocardial ischemia;

[0049] 2) New ischemic ECG changes;

[0050] 3) Pathological Q waves appear in the electrocardiogram;

[0051] 4) Imaging evidence shows new loss of myocardial activity or new local wall motion abnormality;

[0052] 5) Coronary angiography or autopsy ...

Embodiment 2

[0054] Example 2 Sequencing, data analysis and electronic verification

[0055] Sequencing: The miRNA was sequenced using llumina Hiseq2500 / Miseq second-generation high-throughput sequencing technology, and the data processing was completed through the processes of removing adapters, removing low-quality, and decontaminating to obtain the final data. The miRNA sequencing raw data was background-corrected by transcriptome data analysis software, and then the t-test was performed to obtain the P value, and then the Fisher test was used to combine the P values ​​to screen for differentially expressed miRNAs.

[0056] Finally, miR-1274b, miR-520c, and miR-1262 were selected from the candidate differentially expressed miRNAs for later experimental verification.

Embodiment 3

[0057] Embodiment 3 Electronic Verification

[0058] Electronic verification: 3 sets of mRNA data sets (GSE48060, GSE34198 and GSE61145-GPL6884) and 2 sets of miRNA data sets (GSE61741, GSE31568) were screened from the GEO (Gene Expression Omnibus) database, and 3 sets of mRNA data sets (GSE48060, GSE34198 and GSE61145 -GPL6884) has a total of 13,680 genes. We calculated and merged the effect value by using the metaMA package and using the combined P-value method to obtain 612 (FDR1) were found. The results showed that the electronic verification results were consistent with the expression trend of the sequencing results.

[0059] The method for evaluating the efficacy of a single miRNA molecule or a diagnostic model is to establish a receiver operating characteristic (ROC) curve, and judge the ability of diagnosis by calculating the area under the curve (Area UnderCurve). The value of the area under the ROC curve is between 1.0 and 0.5. In the case of AUC>0.5, the closer the...

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Abstract

The invention relates to a reagent for detecting myocardial infarction and the application of the reagent and particularly relates to a reagent for detecting miR-520c and application of the reagent in preparing reagents for detecting myocardial infarction. Because of specificity, sensitivity and the like, conventional myocardial infarction diagnosis biochemical indexes such as creatine kinase, lactic dehydrogenase and myoglobin are limited in clinical application. On the basis of a high-flux sequencing method, a molecular marker miR-520c tightly associated with myocardial infarction is obtained. The molecular marker and conventional biochemical indexes are supplemented by each other, defects of single detection methods are overcome, and bases are made for precise clinical diagnosis and treatment.

Description

technical field [0001] The invention relates to the field of disease diagnosis, in particular to a reagent for detecting myocardial infarction and its application, more specifically to a reagent for detecting miR-520c and its application in the preparation of reagents for diagnosing myocardial infarction. Background technique [0002] Acute myocardial infarction (AMI) refers to acute myocardial ischemic necrosis, which is mostly caused by a sharp reduction or interruption of coronary blood supply on the basis of coronary artery disease, resulting in severe and persistent acute ischemia of the corresponding myocardium. Acute myocardial infarction is the most serious cardiovascular disease. The incidence of myocardial infarction in my country has been on the rise in the past ten years. Early and accurate diagnosis can ensure the immediate start of reperfusion therapy and possibly reduce mortality. However, current diagnosis is based on clinical symptoms, ECG abnormalities and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 李曙光孙锦云
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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