The invention relates to application of a specific protein WIPI1 in an early myocardial infarction diagnostic reagent, the amino acid sequence of the specific protein WIPI1 is shown as SEQ ID NO.1, and during specific detection, after myocardial damage, the content of the specific protein WIPI1 in peripheral blood is increased. Compared with the prior art, the diagnostic reagent provided by the technical scheme of the invention is beneficial to early diagnosis, can reflect the severity of myocardial damage, and can be used as an index for early myocardial infarction diagnosis and prediction, and the detection method is relatively simple and easy to operate, has relatively good detection sensitivity, and has relatively small system errors; the application of the specific protein WIPI1 codedby the WIPI1 gene in a myocardial damage diagnosis reagent can be widely popularized; the kit disclosed by the invention is simple in detection method, low in cost, direct and reliable in detection result and suitable for large-scale screening and diagnosis.