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Acute myocardial infarction diagnosis-oriented biosensor and preparation method thereof

An acute myocardial infarction and biosensor technology, applied in the field of biomedicine, can solve the problems of low-concentration protein detection and long time-consuming detection sensitivity, and achieve important clinical practical value, fast response speed, and high diagnosis rate.

Inactive Publication Date: 2012-05-02
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, a variety of methods have been developed to detect the above proteins, such as electrochemical methods, enzyme-linked immunoassay (ELISA), nano-gold labeling methods, and fluorescence methods, but there are still some key problems that cannot be solved, which limits the application of these technologies. Applications, such as time-consuming, detection sensitivity has not yet adapted to the detection of low-concentration proteins in the early stages of acute myocardial infarction, etc.

Method used

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  • Acute myocardial infarction diagnosis-oriented biosensor and preparation method thereof
  • Acute myocardial infarction diagnosis-oriented biosensor and preparation method thereof
  • Acute myocardial infarction diagnosis-oriented biosensor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Determination of cardiac troponin I using a quasi-one-dimensional silicon nanowire field-effect tube biosensor:

[0060] 1. Using the "top-down" method to prepare a field-effect transistor of a single quasi-one-dimensional silicon nanowire:

[0061] First, use electron beam lithography to process photoresist patterns on the surface of SOI silicon wafers, in which the width of the nanowire region is 50nm;

[0062] Then use ion beam etching (IBE) technology to etch the Si not covered by photoresist;

[0063] Then use ultraviolet lithography to prepare metal electrode patterns, and then use electron beam evaporation equipment to deposit nickel-gold electrodes;

[0064] Finally, the plasma-enhanced chemical vapor deposition (PECVD) technology is used to deposit silicon nitride / silicon oxide double-layer film on the surface of the device, in which the surface of the passivation layer is silicon nitride with a thickness of 100nm, and the silicon oxide film is at the bottom l...

Embodiment 2

[0070] Measurement of cardiac troponin T using a quasi-one-dimensional silicon nanowire field-effect tube biosensor:

[0071] 1. Using the "top-down" method to prepare a field-effect transistor of a single quasi-one-dimensional silicon nanowire:

[0072] First, use electron beam lithography to process photoresist patterns on the surface of SOI silicon wafers, in which the width of the nanowire region is 100nm;

[0073] Then use reactive ion etching (RIE) technology to etch the Si not covered by photoresist;

[0074] Then use ultraviolet lithography to prepare metal electrode patterns, and then use magnetron sputtering to deposit titanium-gold electrodes;

[0075] Finally, the plasma-enhanced chemical vapor deposition (PECVD) technology is used to deposit silicon nitride / silicon oxide double-layer film on the surface of the device, in which the surface of the passivation layer is silicon nitride with a thickness of 500nm, and the silicon oxide film is at the bottom layer with ...

Embodiment 3

[0081] Determination of Creatine Kinase Isoenzymes Using Quasi-One-Dimensional Silicon Nanowire Field-Effect Tube Biosensors:

[0082] 1. Using the "top-down" method to prepare a field-effect transistor of a single quasi-one-dimensional silicon nanowire:

[0083] First, use electron beam lithography to process photoresist patterns on the surface of SOI silicon wafers, in which the width of the nanowire region is 150nm;

[0084] Then use ion beam etching (IBE) technology to etch the Si not covered by photoresist;

[0085] Then use ultraviolet lithography to prepare metal electrode patterns, and then use thermal evaporation equipment to deposit nickel-gold electrodes;

[0086] Finally, the plasma-enhanced chemical vapor deposition (PECVD) technology is used to deposit silicon nitride / silicon oxide double-layer film on the surface of the device, in which the surface of the passivation layer is silicon nitride with a thickness of 300nm, and the silicon oxide film is at the bottom...

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Abstract

The invention discloses an acute myocardial infarction diagnosis-oriented biosensor and a preparation method thereof. The sensor comprises a sensor matrix with a field effect tube (FET) structure, and a micro channel system layer, wherein the sensor matrix comprises a silicon-on-insulator (SOI) silicon chip; the surface of the silicon chip is provided with a passivation layer, silicon nanowires of which the surfaces are coupled with monoclonal antibody modified by acute myocardial infarction marker protein, and electrodes; at least local surfaces of the nanowires are exposed in a micro channel in the micro channel system layer; the micro channel is communicated with a sample solution inlet and a sample solution outlet on the micro channel system layer; and the sensor is prepared by a micro-nano processing method. The sensor can detect various marker proteins in serum by an electrical measurement means so as to acquire the degree of myocardial infarction attack. The biosensor can quickly and sensitively detect the myocardial infarction, has high response speed and diagnostic rate, and is suitable for the field of clinical medical detection.

Description

technical field [0001] The invention relates to a biosensor and a preparation process thereof, in particular to a biosensor with rapid and sensitive response to acute myocardial infarction marker protein and a preparation method thereof, belonging to the technical field of biomedicine. Background technique [0002] Heart disease is currently one of the major diseases affecting people's quality of life. According to the "China Health Statistical Yearbook 2010", in 2009, the incidence and death rate of heart disease among Chinese urban residents was second only to malignant tumors. Myocardial infarction is an important branch of heart disease, and its early, rapid, sensitive and accurate diagnosis is an important way to save the lives of many patients and has extremely important practical value. [0003] Studies have found that the blood levels of cardiac troponin I (cTnI), cardiac troponin T (cTnT), creatine kinase isoenzyme (CK-MB) and myoglobin (MYO) can reflect the severit...

Claims

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Application Information

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IPC IPC(8): G01N33/577
Inventor 程国胜孔涛苏瑞巩张蓓蓓张琦
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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