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Quantum dot joint detection test strip for influenza viruses A and B as well as preparation method and application of quantum dot joint detection test strip

A technology of influenza virus and quantum dots, which is applied in the field of medical testing, can solve the problems of related products that have not yet seen joint detection of influenza virus type A and type B antigens, and achieve the goal of ensuring stability, high sensitivity, and improving accuracy and sensitivity Effect

Pending Publication Date: 2022-04-01
AFFILIATED HOSPITAL OF GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no related product based on the joint detection of influenza virus type A and type B antigens based on quantum dot labeling

Method used

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  • Quantum dot joint detection test strip for influenza viruses A and B as well as preparation method and application of quantum dot joint detection test strip
  • Quantum dot joint detection test strip for influenza viruses A and B as well as preparation method and application of quantum dot joint detection test strip
  • Quantum dot joint detection test strip for influenza viruses A and B as well as preparation method and application of quantum dot joint detection test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Influenza virus type A and type B antigen quantum dot joint detection test strip

[0042] The test strip structure of this embodiment is as follows: figure 1 As shown, it includes a bottom plate 1, on which a sample pad 2, a binding pad 3, a nitrocellulose membrane 4, and a water-absorbing pad 5 are overlapped successively from left to right on the bottom plate, and the binding pad 3 is coated with quantum dot marks Influenza virus type A detection antibody and quantum dot-labeled influenza virus type B detection antibody; the nitrocellulose membrane is provided with a first detection line 401, a second detection line 402 and a quality control line 403, the first detection line 401 is coated with influenza virus type A capture monoclonal antibody, and the second detection line 402 is coated with influenza virus type B capture monoclonal antibody; the quality control line 403 is coated with rabbit anti-mouse IgG polyclonal antibody.

[0043] Wherein, in this e...

Embodiment 2

[0045] The preparation method of embodiment 2 test strips of the present invention

[0046] (1) Preparation of sample pad

[0047] Cut the sample pad into an appropriate size and length that matches the binding pad, spray the sample pad treatment solution on the sample pad at an amount of 50 μL / cm, soak it at room temperature for 1.5 hours, and dry it in a 37°C thermostat for no less than 15 hours Drying for use; The sample treatment solution is a phosphate buffer containing PVP10 (polyvinylpyrrolidone) 0.1wt%, polyethylene glycol-300 1.8wt%, TritonX-100 1.0wt% and 2-hydroxyethylamine 1.6wt% solution (0.015M, pH 7.2).

[0048] (2) Preparation of binding pads

[0049] ①Take 1.5 mg of carboxyl water-soluble quantum dots, and then suspend the quantum dots in 1200 μL of MES buffer solution with a concentration of 0.05M and a pH of 6.0;

[0050] ②Use 0.05M pH6.0 MES buffer to prepare NHS (50mg / ml) and EDC (50mg / ml), take 30μl each and add to quantum dots, the final concentration...

Embodiment 3

[0063] Example 3 Screening experiment of quantum dot complex solution

[0064] Use different quantum dot complex solutions to resuspend quantum dot-labeled influenza virus type A detection antibodies and quantum dot-labeled influenza virus type B detection antibodies, treatment 1: quantum dot complex solutions contain melezitose 2.5wt%, glycine 0.25 wt%, gentamicin sulfate 0.2wt%, cinnamaldehyde 0.12wt% and TritonX-100 0.1wt% phosphate buffer (0.01M, pH7.2); treatment 2: Quantum dot complex solution is containing aminoacetic acid 0.25 wt%, gentamycin sulfate 0.2wt%, cinnamaldehyde 0.12wt% and TritonX-100 0.1wt% phosphate buffer (0.01M, pH 7.2); treatment 3: Quantum dot complex solution containing melezitose 2.5 wt%, gentamicin sulfate 0.2wt%, cinnamaldehyde 0.12wt% and TritonX-100 0.1wt% in phosphate buffer (0.01M, pH 7.2). Each group of test strip samples was prepared according to the method described in Example 2 by using the above-mentioned different quantum dot complex so...

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Abstract

The invention relates to the technical field of medical examination, in particular to a quantum dot joint detection test strip for influenza viruses A and B as well as a preparation method and application of the quantum dot joint detection test strip. The invention discloses a joint detection test strip taking quantum dots as markers and capable of simultaneously detecting influenza viruses A and B. The joint detection test strip comprises a bottom plate, and a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad which are sequentially overlapped on the bottom plate from left to right, the combination pad is coated with an influenza virus type A detection antibody marked by quantum dots and an influenza virus type B detection antibody marked by quantum dots. According to the invention, the quantum dot is used as a marker, is easy to couple with antibody molecules, and is high in fluorescence intensity, strong in stability and not easy to attenuate after excitation, so that the detection has higher sensitivity, and early screening of influenza virus infection is facilitated. The joint detection test strip can be prepared into a strip-shaped structure by being combined with an immunochromatography technology, and is suitable for field screening and bedside detection by being matched with a handheld exciter.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a test strip for combined detection of influenza virus type A and type B quantum dots and a preparation method and application thereof. Background technique [0002] Influenza virus (Influenza virus), referred to as influenza virus, is the main pathogen that causes influenza (Influenza). This virus belongs to Orthomyxoviridae (Orthomyxoviridae), and is further divided into A (A), B (B), C (C), D ( D) Type IV (mBio.2014; 5(2):e00031-14; JVirol.2015; 89(2):1036-42). The clinical symptoms after influenza virus infection include acute high fever, general pain, significant fatigue and respiratory symptoms. Its transmission is mainly through droplets in the air, contact between a susceptible person and an infected person, or contact with contaminated items, with a high incidence in autumn and winter. Due to the differences in their genetic structure, virion structure, antige...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/532
Inventor 潘庆军廖淑珍吴晗沈康远汪书婷
Owner AFFILIATED HOSPITAL OF GUANGDONG MEDICAL UNIV
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