The invention discloses a method for inducing litchi
girdling callus and
peripheral bud differentiation in a field, and relates to the technical field of
plant propagation, the method comprises the following steps:
cutting off the top of a
trunk branch of a litchi growing vertically, and carrying out spiral
girdling below the section of the top until the
girdling depth reaches a wood layer; soaking
absorbent cotton in a
bud differentiation inducer to obtain an induction matrix; after the top section and the girdling opening of the litchi
branch are covered with the induction matrix,
bud differentiation treatment is conducted on the litchi
branch; after bud differentiation treatment is completed, the litchi branches are subjected to
moisture preservation and light shielding treatment, and adventitious buds are obtained. According to the method, wound induction is directly carried out on the litchi plants in the field, the
browning problem in the in-vitro culture process is avoided by utilizing a
plant self-repairing mechanism and combining with local medicament treatment, adventitious bud differentiation around girdling openings is promoted, a stable material source is provided for
field screening of the polyploidy plants, the equipment and labor cost is reduced, and the method is suitable for large-scale popularization and application. Meanwhile, the
survival rate of
genetic variants is improved.