132 results about "Viroid" patented technology

The invention discloses an influenza virus maintenance fluid formula as well as a preparation method and application thereof, and particularly relates to the technical field of biological medicines. The virus maintenance liquid comprises a DMEM / F-12 basal culture medium and effective components, the effective components comprise amino acids, vitamins, salts, a virus proliferation accelerant and an additive, and the virus proliferation accelerant comprises a yeast extract. According to the invention, an influenza virus subunit ELISA detection kit is established, and an influenza virus maintenance solution formula is developed on the basis of a DMEM / F-12 basic culture medium by taking an ELISA detection result at a culture endpoint as a response value. The virus yield of the influenza virus on the MDCK-v cell matrix can be obviously improved, and the production cost is reduced.

The invention belongs to the technical field of biological medicine, and particularly relates to a preparation method of viroid particles displaying scFv antibodies, a product of the viroid particles and application of the viroid particles. The preparation method provided by the invention comprises the following steps: constructing a specific targeting recombinant plasmid, wherein the specific targeting recombinant plasmid is obtained by connecting a vector for expressing virus capsid protein and a coding gene for expressing an scFv antibody display structure through homologous recombination. The vector for expressing the virus capsid protein comprises a coding gene of VSV-G signal peptide, a transmembrane region and an intracellular region, and is as shown in SEQ ID NO. 2. And the coding gene for expressing the scFv antibody display structure is shown as SEQ ID NO. 3-5. According to the preparation method disclosed by the invention, the virus-like particles of which the capsid is a modular component of a specific targeting immune cell are constructed based on a lentivirus system, and 50-90% of infection efficiency can be realized in the immune cell.

The invention discloses a primer probe combination and a kit for detecting shrimp viruses on site, and relates to the technical field of gene detection. The invention provides a primer probe combination for detecting enterocytozoon hepatopenaei, prawn white spot syndrome virus and macrobrachium rosenbergii nodavirus on site. The primer probe combination comprises a primer pair and a probe for detecting enterocytozoon hepatopenaei, the primer pair and the probe are used for detecting the prawn white spot syndrome virus; the invention discloses a primer pair and a probe for detecting macrobrachium rosenbergii nodavirus. The method for detecting the shrimp viruses by adopting the primer probe combination and the kit disclosed by the invention integrates sample pretreatment, nucleic acid extraction, purification, amplification and detection, has better sensitivity and accuracy, is simple, convenient, rapid and time-saving to operate, and plays an important role in on-site rapid detection of research and safety management of the shrimp viruses.

The invention relates to a primer composition and a method for whole genome sequencing of American type porcine reproductive and respiratory syndrome virus and application of the primer composition and the method. The sequences of the primer composition are as shown in SEQ ID No. 1 to SEQ ID No. 35. According to the sequencing method, RNA (Ribonucleic Acid) of a sample to be detected is taken as a template to perform reverse transcription reaction, the primer combination is adopted to perform PCR (Polymerase Chain Reaction) amplification on the whole genome of the American type porcine reproductive and respiratory syndrome virus, and an amplification product is subjected to three-generation sequencing library building and sequencing to perform species identification and genetic typing. The full-length PCR detection method is high in specificity and sensitivity, the efficiency of detecting the whole genome of the virus is greatly improved, rapid and accurate detection of the American type porcine reproductive and respiratory syndrome virus is achieved, accurate typing and evolutionary analysis can be achieved by utilizing the assembled whole genome, and the method is suitable for large-scale popularization and application. Traceability of the reproductive and respiratory syndrome virus is realized, and the method is of great significance to early screening, prevention and control of the American porcine reproductive and respiratory syndrome virus.

The invention discloses a virus-like structure nano adapter and application thereof, on the basis of hepatitis B virus core protein Uniprot P03146 of which nucleic acid binding domains at 145-183 sites of a C tail end are removed, CBP as shown in SEQ ID NO.01 is respectively inserted into a spike part and an N tail end of the hepatitis B virus core protein Uniprot P03146. The invention is constructed based on collagen combined polypeptide CBP designed by bionic decorrin, aims to imitate the interaction between natural decorrin and collagenous fiber, can efficiently cross over corneal epithelium barrier and repair corneal stroma in a non-photo-crosslinking manner, and solves the limitation of existing clinical treatment.

The invention relates to a novel fish virus, indicated to be a Totivirus, which causes mortality in fish, and to methods of detecting said virus in fish, and related uses.

The invention provides a novel vaccine based on an adenovirus vector and application thereof. According to the present invention, the suitable monkey pox virus gene is selected for the first time, and is efficiently integrated with the chimpanzee virus vector (AdC68) to prepare the ideal virus vaccine, and the ideal virus vaccine is suitable for nasal cavity immunization and muscle immunization, and has good nasal cavity immunization effect. The novel monkey pox vaccine can be effectively used for prevention and control of monkey pox virus and vaccinia virus infection, and the two types of viruses are considered.

The invention relates, in general, to synthetic chimeric poxviruses, compositions comprising such viruses, and the development and use of systems and methods for producing such synthetic chimeric poxviruses. The synthetic chimeric poxviruses are well suited for live virus vaccines and pharmaceutical formulations.

The invention discloses an application of Micropterus salmoides Piscidin polypeptide in preparation of an anti-fish virus drug. The amino acid sequence of the polypeptide is as shown in SEQ ID NO. 2 or SEQ ID NO. 3. The research finds that the micropterus salmoides Piscidin is an antibacterial peptide with an antiviral function, the polypeptide synthesized by the artificially synthesized micropterus salmoides Piscidin has the characteristic of inhibiting fish DNA virus replication, has a remarkable inhibiting effect on fish iridovirus replication at low concentration, and can be applied to preparation, research and development of fish antiviral drugs. Research of the antibacterial peptide has important scientific value and application value for further research and development of disease-resistant functional gene products.

The invention relates to a novel fish virus, indicated to be a Totivirus, which causes mortality in fish, and to methods of detecting said virus in fish, and related uses.

The invention provides an RPA-PAND detection kit and detection method for canine parvovirus and canine adenovirus, and belongs to the technical field of canine virus detection.The kit comprises PfAgo protein and a gDNA and MB combination used for detecting canine parvovirus MVC, canine parvovirus CPV and canine adenovirus CAV. The RPA-PAND rapid nucleic acid detection method for detecting canine parvoviruses MVC and CPV and canine adenovirus CAV is successfully established on the basis of PfAgo protein mediated target detection and in combination with the RPA technology, and the method has the advantages of high sensitivity, high specificity, low cost, relatively low position targeting limitation, simple and convenient reaction system, no aerosol pollution, capability of performing multi-channel nucleic acid detection and the like; and a new reagent and a new way are provided for clinical diagnosis and epidemiological monitoring of viruses MVC, CPV and CAV.

The invention provides micropterus salmoides Hepcidin-1 (Ms-Hepcidin-1) and an application of a synthetic polypeptide of the micropterus salmoides Hepcidin-1 in preparation of an anti-fish virus drug. The research finds that the Ms-Hepcidin-1 synthetic polypeptide has a remarkable antiviral effect and is a novel antibacterial peptide with a function of inhibiting fish viruses. The Ms-Hepcidin-1 synthetic polypeptide and the recombinant eukaryotic expression vector of the Ms-Hepcidin-1 synthetic polypeptide can obviously reduce the transcription level of MCP, MMP and DNMT genes and the MCP protein level of LMBV, inhibit the replication of the LMBV and reduce the virus titer, and have an obvious fish virus resisting effect. Moreover, the polypeptide synthesized by Ms-Hepcidin-1 is simple to prepare, low in cost, safe to use and convenient to preserve, can be used as a novel antiviral functional gene product to prepare antiviral drugs for fishes, and has important significance on prevention and control of fish iridovirus diseases.

The invention provides a cell strain for producing engineered viroid particles, a method thereof and the engineered viroid particles, the cell strain is named as SZCV-Con28 and preserved in Guangdong Microbial Culture Collection Center on October 15, 2025, the preservation number is GDMCC No: 67101, and the preservation number is GDMCC No: 67101. A plurality of corresponding cell factories for producing the engineered viroid particles are established by cell lines obtained by introducing different target gRNA elements into the cell strain, so that the corresponding engineered viroid particles can be continuously produced in batches. According to the invention, large-scale preparation can be realized through cell expanding culture, the capacity limitation is overcome, the production process flow is simplified, and the consumption of production raw materials is reduced.

The invention provides a virus-like vector lysis solution and a virus-like vector lysis method. The virus-like vector lysis solution comprises Tris, EDTA (Ethylene Diamine Tetraacetic Acid), NaCl, TritonX-100, sodium deoxycholate, SDS (Sodium Dodecyl Sulfate), Tween 20 and a protease inhibitor mixture. Cas9 protein is wrapped in the viroid vector; the acquisition method of the viroid vector comprises the step of transfecting plasmids containing a structural gene sequence for expressing the viroid vector into virus producing cells to obtain the viroid vector. The cracking method of the viroid vector comprises the following steps: S1, using: carrying out cracking treatment on a viroid vector solution by using the cracking solution; and S2, performing Cas9 protein content detection on substances released after the carrier is cracked. The invention establishes a formula of the special lysate for VLP and a corresponding cracking method, and ensures high cracking efficiency.

The present application belongs to the technical field of biological medicine, and particularly relates to a thiol phenylboronic acid mediated nano-enzyme immunochromatography reagent and application thereof. The present application utilizes the broad-spectrum binding ability of MPBA to the glycosylated molecules on the surface of pathogens, and combines the colorimetric catalytic double enhancement effect of the virus-like biomimetic magnetic nano-enzyme (FeAu@AuIr), to improve the detection sensitivity. The probe takes Fe3O4 magnetic nanoparticles as the core, and sequentially loads large-diameter gold nanoparticles (Au) and gold-iridium alloy (AuIr) with high catalytic activity, to enhance the pathogen capture capacity and signal amplification effect. Combined with the antibody modified immunochromatography strip, Streptococcus pneumoniae, Pseudomonas aeruginosa and SARS-CoV-2 can be simultaneously detected, and the detection sensitivity is greatly improved. Through verification of 170 clinical samples, the present application has good application prospect in on-site rapid detection, and provides an efficient and low-cost solution for precise screening of respiratory tract infectious pathogens.

The invention discloses a tannic acid functionalized micromotor with functions of enhancing bacterium capture and bacteriostasis, and a preparation method and application thereof. The micromotor takes Cu7S4 / vSiO2 particles with a virus-like structure as a carrier, and tannic acid is loaded on the surface of the carrier through a self-assembly method. On one hand, TA has an antibacterial effect, and rich peptidoglycan on cell walls of gram-positive bacteria has a high affinity effect; on the other hand, a virus-like structure with a spike structure has relatively high adhesion to bacteria, and the micromotor Cu7S4 / vSiO2 / TA has an enhanced capture effect on gram-positive bacteria; in addition, the photothermal effect and self-driving generated by Cu7S4 under the irradiation of infrared light can cooperate with TA to realize efficient sterilization.

The invention provides an oligopeptide with a fish virus resisting effect, and the oligopeptide is used as a feed additive or a liquid protein preparation for injection to kill viruses or inhibit the proliferation of the viruses, so that the treatment of the fish virus diseases is realized. The amino acid sequence of the oligopeptide is SEQ ID NO: 1. The Pt5-1c oligopeptide provided by the invention reduces the cytopathic effect degree of GCRV virus infection on EPC, and shows that the Pt5-1c oligopeptide has an important antiviral effect. By adding the Pt5-1c oligopeptide into the feed or injecting the Pt5-1c oligopeptide into the fish, the purpose of preventing and treating viral diseases of the fish can be achieved.

The present invention relates to the field of oncolytic viruses and in particular to a recombinant rhabdovirus, such as vesicular stomatitis virus encoding in its genome for a gasdermin. The invention is further directed to the use of the recombinant virus in the treatment of cancer and also to methods for producing such viruses.

The invention discloses a pelteobagrus fulvidraco brain tissue cell line PYCB sensitive to viruses and application thereof, and belongs to the technical field of aquatic organism cell culture and disease prevention and control. The preservation number of the Pelteobagrus fulvidraco brain tissue cell line PYCB is CCTCC (China Center For Type Culture Collection) NO: C2025148. The Pelteobagrus fulvidraco brain tissue cell line PYCB is successfully constructed by taking the Pelteobagrus fulvidraco brain tissue as an object, and the cell line can be continuously and stably subcultured, is high in proliferation speed and simple in culture method, is sensitive to Pelteobagrus fulvidraco small RNA viruses and Pelteobagrus fulvidraco stem casing viruses, and can be used for isolated culture of viruses. The invention provides an important research platform and an experimental material for separating and identifying fish viruses and virus vaccines and developing medicines.

The present invention provides a one-step multiplex fluorescence quantitative PCR kit for detecting and distinguishing poxviruses. It comprises a quantitative PCR reaction solution, an enzyme mixture, a primer-probe mixture, a standard, a positive control substance, and a negative control substance. The poxviruses include monkeypox virus, cowpox virus, and varicella virus. The present invention designs highly specific primer probes according to the highly conserved nucleotide sequences of the three poxviruses and the internal standard RNase P, and simultaneously detects and distinguishes the presence of monkeypox virus, cowpox virus, and varicella virus from samples such as blood, follicle fluid, and rash swabs through a PCR reaction, and uses RNase P as the internal standard for evaluating the amplification system, which is simpler, faster, and less costly than the single-plex fluorescence quantitative PCR method, and has better sensitivity, specificity, and repeatability. The viruses to be tested are fully enclosed for detection and single-tube real-time quantification, and the three poxviruses can be efficiently and quickly screened and identified according to the number of copies of the virus infection.

The present invention relates to an isolated nucleotide sequence comprising: a) cDNA of two strands of a target double-stranded RNA (dsRNA), separated by an autocatalytic intron flanking exon fragments, and b) a plant viroid, wherein element (a) is inserted into the plant viroid sequence. The expression of this nucleotide sequence together with a tRNA ligase in a host cell (such as E. coli) allows the production of large amounts of dsRNA specific for a target gene. This dsRNA can be used in interference RNA technology to silence gene expression. In addition, the present invention also relates to a method for preparing said dsRNA.

The present invention belongs to the field of biomedicine, and particularly relates to a fibroblast cell line of mallard duck embryos, a construction method thereof, and an application thereof. Through a large amount of creative work by the inventors, the present invention for the first time isolated and obtained a fibroblast cell line from mallard duck embryos, and successfully domesticated it in serum-free suspension. The name of the cell line is DEF-Sfs, fibroblast suspension cells of mallard duck embryos, which was deposited at the China Center for Type Culture Collection, Wuhan University, Wuhan, China on June 28, 2024, with the deposit number of CCTCC NO: C2024191. This cell line can be used for culturing, isolating, and detecting avian viruses, preparing avian vaccines, and screening drugs for preventing or treating diseases caused by avian virus infections.

This invention provides the application of Hepcidin-1 (Ms-Hepcidin-1) and its synthetic polypeptide in the preparation of antiviral drugs for largemouth bass. The invention reveals that the Ms-Hepcidin-1 synthetic polypeptide exhibits significant antiviral activity and is a novel antimicrobial peptide with the function of inhibiting fish viruses. Both the Ms-Hepcidin-1 synthetic polypeptide and its recombinant eukaryotic expression vector can significantly reduce the transcriptional levels of MCP, MMP, and DNMT genes and the MCP protein level of LMBV, inhibit LMBV replication, and reduce viral titers, demonstrating significant antiviral activity against fish viruses. Furthermore, the polypeptide synthesized from Ms-Hepcidin-1 is simple to prepare, low in cost, safe to use, and easy to store. As a novel antiviral functional gene product, it can be applied to the preparation of antiviral drugs for fish, which is of great significance for the prevention and control of iridovirus disease in fish.

The present invention relates nucleic acid molecules and concatemers containing spacer sequences useful for the efficient packaging of viral particles so as to minimize the incorporation of contaminant nucleic acids into these vectors, as well as methods of producing such viral particles.

The present application relates to the fields of biotechnology and agricultural fishery, and discloses application of star anise water extract in preparation of an inhibitor of CyHV-2, and the technical scheme finds that the star anise water extract can effectively inhibit the infection and replication of CyHV-2 virus in GiCF cells. The present application firstly applies the star anise water extract to prevent and treat the infection of CyHV-2 (CyHV-2), thereby providing a new treatment scheme for CyHV-2 virus. The star anise water extract can effectively reduce the copy number of CyHV-2 virus, and the star anise water extract can be used to prepare a medicine for preventing and treating the infection of CyHV-2, and a medicine for treating viral hematopoietic organ necrosis disease of fish.

The present invention provides a method of electrotransfection-free batch preparation of an immune cell therapeutic product, the CD7 gene of the immune cell itself being knocked out and simultaneously expressing a CD7-targeted chimeric antigen receptor, comprising the production of engineered viroid particles by a stable cell line, the knockout of the CD7 gene in the immune cell using the engineered viroid particles, and the preparation of the therapeutic product of the immune cell, the preparation of the therapeutic product of the immune cell, the preparation of the therapeutic product of the immune cell, and the preparation of the therapeutic product of the immune cell. The CD7 CAR retroviral vector is used for transducing immune cells, so that the immune cell treatment product is obtained. Aiming at the difficulties of high cost, poor universality and high failure risk of CRISPR application electrotransfection technology in current CAR-T cell preparation, the eVLP technology is introduced to carry out efficient and convenient gene editing, so that the safety is guaranteed, the cost is saved, the operation complexity is reduced, and meanwhile, the success rate of immune cell preparation is greatly improved. Based on clinical requirements, a cell therapy product of CD7 KO + CD7 CAR T is jointly constructed by combining a CD7 eVLP vector with a retrovirus of CD7 CAR.

A composition for suppression of avian viral epidemic diseases comprising a horseradish extract and a cinnamon extract is provided. The causative virus of the avian viral epidemic disease is preferabl

The invention relates to reagents, compositions, methods, and kits for detecting a hop latent viroid in a plant.

The invention provides a double-lock type viroid fluorescent nanoprobe for monitoring a virus infection process, and a preparation method and application of the double-lock type viroid fluorescent nanoprobe. The probe is composed of a Japanese encephalitis virus envelope E protein modified polyamidoamine dendrimer, and the polymer is loaded with a vRNA and depurine / depyrimidine endonuclease 1 (APE1) dual activated molecular beacon. The probe can penetrate through a blood brain barrier and enter virus-infected cells to release molecular beacons complementary with vRNA, and the molecular beacons are selectively split by APE1, so that specific fluorescence signal amplification in virus-infected lesions is realized. The double-activation strategy greatly improves the spatial accuracy and sensitivity of virus infection focus imaging. The invention provides a feasible method for monitoring the in-vivo virus infection level in real time and evaluating the effectiveness of antiviral drugs.