79 results about "Viroid" patented technology

The invention discloses a primer probe combination and a kit for detecting shrimp viruses on site, and relates to the technical field of gene detection. The invention provides a primer probe combination for detecting enterocytozoon hepatopenaei, prawn white spot syndrome virus and macrobrachium rosenbergii nodavirus on site. The primer probe combination comprises a primer pair and a probe for detecting enterocytozoon hepatopenaei, the primer pair and the probe are used for detecting the prawn white spot syndrome virus; the invention discloses a primer pair and a probe for detecting macrobrachium rosenbergii nodavirus. The method for detecting the shrimp viruses by adopting the primer probe combination and the kit disclosed by the invention integrates sample pretreatment, nucleic acid extraction, purification, amplification and detection, has better sensitivity and accuracy, is simple, convenient, rapid and time-saving to operate, and plays an important role in on-site rapid detection of research and safety management of the shrimp viruses.

The invention discloses a virus-like structure nano adapter and application thereof, on the basis of hepatitis B virus core protein Uniprot P03146 of which nucleic acid binding domains at 145-183 sites of a C tail end are removed, CBP as shown in SEQ ID NO.01 is respectively inserted into a spike part and an N tail end of the hepatitis B virus core protein Uniprot P03146. The invention is constructed based on collagen combined polypeptide CBP designed by bionic decorrin, aims to imitate the interaction between natural decorrin and collagenous fiber, can efficiently cross over corneal epithelium barrier and repair corneal stroma in a non-photo-crosslinking manner, and solves the limitation of existing clinical treatment.

The invention relates to a novel fish virus, indicated to be a Totivirus, which causes mortality in fish, and to methods of detecting said virus in fish, and related uses.

The invention provides a novel vaccine based on an adenovirus vector and application thereof. According to the present invention, the suitable monkey pox virus gene is selected for the first time, and is efficiently integrated with the chimpanzee virus vector (AdC68) to prepare the ideal virus vaccine, and the ideal virus vaccine is suitable for nasal cavity immunization and muscle immunization, and has good nasal cavity immunization effect. The novel monkey pox vaccine can be effectively used for prevention and control of monkey pox virus and vaccinia virus infection, and the two types of viruses are considered.

The invention discloses an application of Micropterus salmoides Piscidin polypeptide in preparation of an anti-fish virus drug. The amino acid sequence of the polypeptide is as shown in SEQ ID NO. 2 or SEQ ID NO. 3. The research finds that the micropterus salmoides Piscidin is an antibacterial peptide with an antiviral function, the polypeptide synthesized by the artificially synthesized micropterus salmoides Piscidin has the characteristic of inhibiting fish DNA virus replication, has a remarkable inhibiting effect on fish iridovirus replication at low concentration, and can be applied to preparation, research and development of fish antiviral drugs. Research of the antibacterial peptide has important scientific value and application value for further research and development of disease-resistant functional gene products.

The invention relates to a novel fish virus, indicated to be a Totivirus, which causes mortality in fish, and to methods of detecting said virus in fish, and related uses.

The invention provides an RPA-PAND detection kit and detection method for canine parvovirus and canine adenovirus, and belongs to the technical field of canine virus detection.The kit comprises PfAgo protein and a gDNA and MB combination used for detecting canine parvovirus MVC, canine parvovirus CPV and canine adenovirus CAV. The RPA-PAND rapid nucleic acid detection method for detecting canine parvoviruses MVC and CPV and canine adenovirus CAV is successfully established on the basis of PfAgo protein mediated target detection and in combination with the RPA technology, and the method has the advantages of high sensitivity, high specificity, low cost, relatively low position targeting limitation, simple and convenient reaction system, no aerosol pollution, capability of performing multi-channel nucleic acid detection and the like; and a new reagent and a new way are provided for clinical diagnosis and epidemiological monitoring of viruses MVC, CPV and CAV.

The invention provides micropterus salmoides Hepcidin-1 (Ms-Hepcidin-1) and an application of a synthetic polypeptide of the micropterus salmoides Hepcidin-1 in preparation of an anti-fish virus drug. The research finds that the Ms-Hepcidin-1 synthetic polypeptide has a remarkable antiviral effect and is a novel antibacterial peptide with a function of inhibiting fish viruses. The Ms-Hepcidin-1 synthetic polypeptide and the recombinant eukaryotic expression vector of the Ms-Hepcidin-1 synthetic polypeptide can obviously reduce the transcription level of MCP, MMP and DNMT genes and the MCP protein level of LMBV, inhibit the replication of the LMBV and reduce the virus titer, and have an obvious fish virus resisting effect. Moreover, the polypeptide synthesized by Ms-Hepcidin-1 is simple to prepare, low in cost, safe to use and convenient to preserve, can be used as a novel antiviral functional gene product to prepare antiviral drugs for fishes, and has important significance on prevention and control of fish iridovirus diseases.

The invention provides a cell strain for producing engineered viroid particles, a method thereof and the engineered viroid particles, the cell strain is named as SZCV-Con28 and preserved in Guangdong Microbial Culture Collection Center on October 15, 2025, the preservation number is GDMCC No: 67101, and the preservation number is GDMCC No: 67101. A plurality of corresponding cell factories for producing the engineered viroid particles are established by cell lines obtained by introducing different target gRNA elements into the cell strain, so that the corresponding engineered viroid particles can be continuously produced in batches. According to the invention, large-scale preparation can be realized through cell expanding culture, the capacity limitation is overcome, the production process flow is simplified, and the consumption of production raw materials is reduced.

The present application belongs to the technical field of biological medicine, and particularly relates to a thiol phenylboronic acid mediated nano-enzyme immunochromatography reagent and application thereof. The present application utilizes the broad-spectrum binding ability of MPBA to the glycosylated molecules on the surface of pathogens, and combines the colorimetric catalytic double enhancement effect of the virus-like biomimetic magnetic nano-enzyme (FeAu@AuIr), to improve the detection sensitivity. The probe takes Fe3O4 magnetic nanoparticles as the core, and sequentially loads large-diameter gold nanoparticles (Au) and gold-iridium alloy (AuIr) with high catalytic activity, to enhance the pathogen capture capacity and signal amplification effect. Combined with the antibody modified immunochromatography strip, Streptococcus pneumoniae, Pseudomonas aeruginosa and SARS-CoV-2 can be simultaneously detected, and the detection sensitivity is greatly improved. Through verification of 170 clinical samples, the present application has good application prospect in on-site rapid detection, and provides an efficient and low-cost solution for precise screening of respiratory tract infectious pathogens.

The invention provides an oligopeptide with a fish virus resisting effect, and the oligopeptide is used as a feed additive or a liquid protein preparation for injection to kill viruses or inhibit the proliferation of the viruses, so that the treatment of the fish virus diseases is realized. The amino acid sequence of the oligopeptide is SEQ ID NO: 1. The Pt5-1c oligopeptide provided by the invention reduces the cytopathic effect degree of GCRV virus infection on EPC, and shows that the Pt5-1c oligopeptide has an important antiviral effect. By adding the Pt5-1c oligopeptide into the feed or injecting the Pt5-1c oligopeptide into the fish, the purpose of preventing and treating viral diseases of the fish can be achieved.

The present invention relates to the field of oncolytic viruses and in particular to a recombinant rhabdovirus, such as vesicular stomatitis virus encoding in its genome for a gasdermin. The invention is further directed to the use of the recombinant virus in the treatment of cancer and also to methods for producing such viruses.

The invention discloses a pelteobagrus fulvidraco brain tissue cell line PYCB sensitive to viruses and application thereof, and belongs to the technical field of aquatic organism cell culture and disease prevention and control. The preservation number of the Pelteobagrus fulvidraco brain tissue cell line PYCB is CCTCC (China Center For Type Culture Collection) NO: C2025148. The Pelteobagrus fulvidraco brain tissue cell line PYCB is successfully constructed by taking the Pelteobagrus fulvidraco brain tissue as an object, and the cell line can be continuously and stably subcultured, is high in proliferation speed and simple in culture method, is sensitive to Pelteobagrus fulvidraco small RNA viruses and Pelteobagrus fulvidraco stem casing viruses, and can be used for isolated culture of viruses. The invention provides an important research platform and an experimental material for separating and identifying fish viruses and virus vaccines and developing medicines.

This invention provides the application of Hepcidin-1 (Ms-Hepcidin-1) and its synthetic polypeptide in the preparation of antiviral drugs for largemouth bass. The invention reveals that the Ms-Hepcidin-1 synthetic polypeptide exhibits significant antiviral activity and is a novel antimicrobial peptide with the function of inhibiting fish viruses. Both the Ms-Hepcidin-1 synthetic polypeptide and its recombinant eukaryotic expression vector can significantly reduce the transcriptional levels of MCP, MMP, and DNMT genes and the MCP protein level of LMBV, inhibit LMBV replication, and reduce viral titers, demonstrating significant antiviral activity against fish viruses. Furthermore, the polypeptide synthesized from Ms-Hepcidin-1 is simple to prepare, low in cost, safe to use, and easy to store. As a novel antiviral functional gene product, it can be applied to the preparation of antiviral drugs for fish, which is of great significance for the prevention and control of iridovirus disease in fish.

The present invention relates nucleic acid molecules and concatemers containing spacer sequences useful for the efficient packaging of viral particles so as to minimize the incorporation of contaminant nucleic acids into these vectors, as well as methods of producing such viral particles.

The present application relates to the fields of biotechnology and agricultural fishery, and discloses application of star anise water extract in preparation of an inhibitor of CyHV-2, and the technical scheme finds that the star anise water extract can effectively inhibit the infection and replication of CyHV-2 virus in GiCF cells. The present application firstly applies the star anise water extract to prevent and treat the infection of CyHV-2 (CyHV-2), thereby providing a new treatment scheme for CyHV-2 virus. The star anise water extract can effectively reduce the copy number of CyHV-2 virus, and the star anise water extract can be used to prepare a medicine for preventing and treating the infection of CyHV-2, and a medicine for treating viral hematopoietic organ necrosis disease of fish.

The present invention provides a method of electrotransfection-free batch preparation of an immune cell therapeutic product, the CD7 gene of the immune cell itself being knocked out and simultaneously expressing a CD7-targeted chimeric antigen receptor, comprising the production of engineered viroid particles by a stable cell line, the knockout of the CD7 gene in the immune cell using the engineered viroid particles, and the preparation of the therapeutic product of the immune cell, the preparation of the therapeutic product of the immune cell, the preparation of the therapeutic product of the immune cell, and the preparation of the therapeutic product of the immune cell. The CD7 CAR retroviral vector is used for transducing immune cells, so that the immune cell treatment product is obtained. Aiming at the difficulties of high cost, poor universality and high failure risk of CRISPR application electrotransfection technology in current CAR-T cell preparation, the eVLP technology is introduced to carry out efficient and convenient gene editing, so that the safety is guaranteed, the cost is saved, the operation complexity is reduced, and meanwhile, the success rate of immune cell preparation is greatly improved. Based on clinical requirements, a cell therapy product of CD7 KO + CD7 CAR T is jointly constructed by combining a CD7 eVLP vector with a retrovirus of CD7 CAR.

A composition for suppression of avian viral epidemic diseases comprising a horseradish extract and a cinnamon extract is provided. The causative virus of the avian viral epidemic disease is preferabl

The invention relates to a multi-antigen component PEI lipid delivery system and an anti-avian influenza virus multivalent vaccine comprising the same, belongs to the technical field of biological medicines, and provides a delivery system consisting of PEI, squalene, cholesterol and mPEG. The preparation method comprises the following steps of: constructing mRNA (messenger ribonucleic acid) molecules and a composite vaccine antigen system consisting of prokaryotic expression purified H7N9 avian influenza NP antigen, loading the composite vaccine antigen system into a delivery system to obtain the nanoparticles with a virus-like structure, and displaying the HA protein antigen and the NP antigen in the structure of the nanoparticles in a particle form by the nanoparticles, so that the nanoparticles can be used for preparing the H7N9 avian influenza NP / H7N9 avian influenza NP / H7N9 avian influenza NP / H7N9 avian influenza virus. And the mRNA molecule for coding the NA antigen, which is combined with the mRNA molecule, can be delivered into a cell in a manner of fusing lipid and a cell membrane, so that the mRNA molecule can be effectively expressed to induce a body to generate specific antibody reactions aiming at HA, NA and NP, and the body can also generate specific CTL reactions aiming at HA, NA and NP of different influenza virus strain subtypes.

The application belongs to the field of pharmaceutical chemical industry and relates to a cyclic peptide compound shown as formula M or a stereoisomer, a tautomer or a mixture thereof of the compound, a pharmaceutically acceptable salt, a polymorph, a co-crystal or a solvate of the compound, or a stable isotope derivative, a metabolite or a prodrug of the compound. The application also relates to a method for synthesizing the cyclic peptide compound and an intermediate. The provided cyclic peptide compound has a strong inhibitory effect on a coronavirus 3CL protease and a small RNA virus (for example, an enterovirus EV71 virus) 3C protease. After cell level activity testing, the provided cyclic peptide compound has an obvious inhibitory effect on EV71 virus and coronavirus and the like, indicating that the cyclic peptide compound shown as formula M can produce a viral inhibitory effect at the cell level by inhibiting viral protease activity, and has a good application prospect as an antiviral drug.

The invention belongs to the technical field of aquaculture, and relates to a new application of capsaicin, in particular to an application of capsaicin in preparation of drugs and / or feed additives for preventing and treating fish virus infection. The core of the invention lies in that the capsaicin has an obvious protection effect on infection of spring viraemia of carp virus (SVCV), grass carp reovirus (GCRV) and herpesvirus type 2 (CyHV-2), and can obviously inhibit transcription of SVCV, GCRV and CyHV-2 virus genes and expression of virus proteins. The capsaicin-containing feed additive and / or medicine can be used for preventing and treating fish virus diseases, and has the advantages of high protection efficiency and environmental friendliness.

The application discloses a kind of MS2 virus-like particles based on capsid protein site-directed mutation and its preparation method and application, belong to molecular biological technique field.The amino acid of the beta sheet interface of capsid protein sequence, the amino acid of the interface when two capsid proteins molecules assemble, the carboxyl end site of capsid protein is subjected to site-directed mutation, obtain the mutant that can significantly improve the assembly efficiency and stability of capsid protein dimer.Utilize the mutant to construct MS2 virus-like particles, further by the design of mature enzyme and capsid protein expression element, control mature enzyme weak expression, capsid protein strong expression, to improve assembly efficiency.The application improves the assembly efficiency of MS2 virus by the above strategy, improves the proportion of wrapped RNA, enhances the stability of virus-like particle, greatly prolongs the stable storage time of RNA, improves the purity of wrapped RNA.

This invention belongs to the field of aquaculture technology and relates to a novel use of capsaicin, particularly its use in the preparation of drugs and / or feed additives for the prevention and treatment of fish viral infections. The core of this invention lies in the significant protective effect of capsaicin against carp spring viremia virus (SVCV), grass carp reovirus (GCRV), and carp herpesvirus type 2 (CyHV-2) infections, effectively inhibiting the transcription of SVCV, GCRV, and CyHV-2 viral genes and the expression of viral proteins. Feed additives and / or drugs containing capsaicin can be used for the prevention and treatment of fish viral diseases, offering advantages such as high protective efficiency and environmental friendliness.

The application provides visible light catalytic cross-linked virus-like ruthenium oxide nanoparticles for efficient transmembrane treatment of keratoconus, which are mainly prepared from cetyltrimethylammonium bromide, sodium hydroxide, tetraethyl orthosilicate, cyclohexane, ruthenium chloride, phenylalanine and pharmaceutically acceptable components or adjuvants, and have virus-like nanoscale and rough morphology. The virus-like ruthenium oxide nanoparticles can efficiently cross the corneal epithelium, enter the corneal stroma, and react with ammonium persulfate to generate free radicals, oxidize the tyrosine groups in the collagen fibers of the cornea to form a di-tyrosine bond, and cross-link the collagen fibers of the cornea. The application does not require ultraviolet light irradiation, is convenient to use, is non-invasive and reduces the discomfort of patients during treatment, and has important significance for improving the compliance and quality of life of keratoconus patients.

The application discloses a kind of single-chain small deoxyribonucleic acid targeting multiple viruses and application thereof.The nucleotide sequence of the single-chain small deoxyribonucleic acid is 5'-ACAGGGTTTTCACCCTTCCTT-3', which targets the highly conserved region of 9 kinds of viruses infecting tomato, realizes the synergistic silencing effect of "one target multi-control". Compared with traditional ds-sRNA, the ss-sDNA of the application shows higher stability and viroid RNA interference efficiency in plants. Experiments prove that, compared with ds-sRNA, 20-25 μM concentration of ss-sDNA foliar spraying or stem injection can reduce the titers of 9 kinds of viruses and plant disease index by 10%-39% and 3.3%-17.4% respectively. The application provides a new type of nucleic acid pesticide for viroid disease, which is efficient, broad-spectrum and environment-friendly.

The application discloses a macrovirus group data analysis method, and belongs to the technical field of macrovirology. The method comprises the following steps: sequence quality control, sequence assembly, sequence clustering, virus sequence identification, virus sequence checking, virus abundance calculation, species annotation, virus lifestyle judgment, virus host prediction and virus auxiliary metabolism gene analysis. The application uses Trimmomatic software, BWA-MEN, Megahit software, CD-HIT and other tools to execute the analysis process of macrovirus group data. Practice proves that the application can accurately identify and annotate virus species, comprehensively and systematically deeply analyze and mine macrovirus group data, the steps are simple and clear, the analysis time is short, and the effect of macrovirus identification research is greatly improved.

The utility model provides a fish multi-virus antigen joint detection kit, which relates to the technical field of antigen detection, and comprises a plurality of outer shells, each outer shell is provided with a connecting assembly used for connecting two outer shells together, the top of each outer shell is provided with an observation window and a sample adding hole, and the observation window is provided with a plurality of through holes. One end of each outer shell is open, an inner shell is detachably installed in each outer shell in a sliding mode, a drawing handle is fixedly installed at the outer end of each inner shell, the free end of each drawing handle extends out of the corresponding outer shell, and a locking assembly is arranged between the inner end of each inner shell and the other end of the corresponding outer shell. According to the fish virus detection kit, the number of the outer shells and the types of the detection test strips can be freely combined according to the number and the types of fish viruses to be detected, and waste of the outer shells and the detection test strips is avoided.

The invention discloses a single-stranded small desoxyribonucleic acid targeting multiple viruses and application of the single-stranded small desoxyribonucleic acid. The nucleotide sequence of the single-stranded small desoxyribonucleic acid is 5 '-ACAGGGTTTTCACCCTTCCT-3', and the single-stranded small desoxyribonucleic acid targets a highly conserved region of nine types of viruses of the infected tomato, so that a one-target multi-control synergistic silencing effect is achieved. Compared with the traditional ds-sRNA, the s-sDNA disclosed by the invention shows higher stability and virus-like RNA interference efficiency in a plant body. Experiments prove that compared with ds-sRNA, the titers of 9 types of viruses and the disease index of a plant can be respectively reduced by 10%-39% and 3.3%-17.4% by foliar spraying or stalk injection of the s-sDNA with the concentration of 20-25 mu M. The invention provides an efficient, broad-spectrum and environment-friendly novel nucleic acid pesticide for viroid diseases.

This invention relates to methods of prophylactically or therapeutically treating viral infections particularly in the lower respiratory tract. Treatment of coronavirus infections is particularly contemplated. The invention also relates to the use of BLIS-producing Streptococcus strains, extracts and exudates thereof, and compositions containing same in the prevention, reduction, or treatment of such virus infections.