33 results about "Virus types" patented technology

The application provides a tobacco virus classification model construction method based on tobacco leaf hyperspectrum, and belongs to the technical field of tobacco virus classification models. Fractional differential spectrum enhancement processing is adopted, persistent homology eigenvalues and Betti number sequence values are calculated through spectrum topological manifold embedding of enhanced spectrum data, dimension reduction is performed through kernel principal component analysis to obtain topological embedding feature vectors, fusion feature vectors are output through a multimodal attention fusion module in combination with thermal infrared temperature field data and chlorophyll fluorescence kinetic curve data, data enhancement is performed on minority class samples by using a spectral generative adversarial network, the fusion feature vectors are input into a virus classification and recognition model adopting a focal loss function and a dynamic network adjustment mechanism for training, virus type classification and disease severity grade recognition of tobacco leaves to be tested are realized, and the problem that different types of tobacco viruses cannot be accurately recognized and distinguished in the early stage of disease is solved.

The invention discloses a neutralizing swine monoclonal antibody pO18-40 and a neutralizing swine monoclonal antibody pO18-43 for foot and mouth disease virus type O. The amino acid sequences of a heavy chain variable region (VH) and a light chain variable region (VL) of the antibody pO18-40 are respectively as shown in SEQ ID No. 1 and SEQ ID No. 2; the amino acid sequences of VH and VL of the antibody pO18-43 are respectively as shown in SEQ ID No. 3 and SEQ ID No. 4. The antibody obtained by the invention is a full-swine-source antibody, can specifically neutralize the classical strain of the O-type foot-and-mouth disease virus, and can clearly distinguish the classical strain of the O / Cathay topological type from the variant strain of the O / Cathay topological type. A key antigen epitope recognized by the antibody is located at the 149th amino acid of a VP1 protein G-H ring, and the site is a key site of O / Cathay strain antigen variation and vaccine immune protection. The antibody provided by the invention provides an important tool and theoretical basis for serological detection of O-type FMDV, vaccine immune effect evaluation and optimal design of broad-spectrum vaccines.

The virus of the present disclosure is a multiple mutant virus in which two or more genes of herpes simplex virus type 2 (HSV-2) are altered and the alteration of the genes is impaired or reduced in gene function.

Described herein are systems and methods that may be used to differentially detect viral serotype specific nucleic acid. For example, these systems may comprise multiple DNA-nanostructures, capture oligonucleotides and protector oligonucleotides, wherein each DNA-nanostructure and its associated capture oligonucleotide and protector oligonucleotide are specific for a unique viral type or serotype.

The application provides a 1-substituted cinnamoyl-2-oxo lycorine compound, a preparation method and application thereof. The structure of the compound is shown in the following formula I: wherein the benzene ring has one or more R groups which are substituents on the benzene ring, and the R group is selected from hydrogen, C 1‑6 alkyl, C 1‑6 alkylalkoxy, halogen, halogenated C 1‑6 alkyl, halogenated C 1‑6 alkoxy, nitro and dioxo. The compound shows excellent antiviral effect, especially for herpes simplex virus type I (HSV-1), and the compound has better safety.

The invention discloses a primer group and a kit for simultaneously detecting a panda aleutian virus and a panda canine distemper virus Asia type 6 and application of the primer group and the kit, and belongs to the technical field of virus detection of endangered wild animals. The invention provides a primer group and a kit for specifically recognizing the aleutian disease virus and the canine distemper virus Asia 6 type of the panda, and detection can be completed within 20 minutes under the condition of constant temperature of 39 DEG C; according to the present invention, the method has characteristics of rapid detection, good specificity, high sensitivity and the like, does not produce the cross reaction with other common pathogens, does not produce the false positive phenomenon due to the generation of the primer dimer, has characteristics of rapid detection, good specificity, high sensitivity and the like, and has characteristics of simple and easy operation, and on-site detection without special detection personnel; the method is rapid and accurate in detection, has a good application effect in preliminary application, and provides a method for rapid diagnosis, virus traceability, epidemic disease monitoring and early warning of a wild animal epidemic disease source and the like in zoos and wild animal rescue institutions.

The invention provides a molecular diagnosis method for avian leukosis virus and Marek's disease virus mixed infection. The molecular diagnosis method comprises the following steps: (S01) collecting diseased material tissues; (S02) carrying out nucleic acid extraction on the sick material tissue of the sick chicken; (S03) detecting the nucleic acid by using specific primers of avian leukosis and Marek's disease; (S04) preparing a PCR (Polymerase Chain Reaction) detection system, and respectively adding ALV-env gene amplification primers and MDV-meq gene amplification primers for gene amplification; (S05) carrying out 1% agarose gel electrophoresis detection on the amplification product in the step (4), and carrying out sequence determination after determining a target band; (S06) carrying out BLAST comparison on the sequences obtained by sequencing, carrying out gene sequence analysis and drawing an evolutionary tree; and (S07) determining the virus type according to the homology and the evolutionary branches, so as to realize rapid diagnosis of ALV and MDV mixed infection, strain characteristic analysis and exclusion of other similar epidemic diseases, and provide technical support for accurate prevention and control of AL and MD in the poultry industry, provenance purification and epidemiological investigation.

The invention relates to a pediatric respiratory tract infection rapid diagnosis device. By setting a clinical manifestation database of various children respiratory diseases, data can be filled into the database according to external clinical manifestations of various children under different respiratory viruses; and the specific child respiratory tract infection virus type is determined through preliminary clinical manifestation data symptom judgment and further saliva detection, so that the diagnosis rhythm is accelerated, and the diagnosis accuracy is improved.

This invention discloses a primer set, reagent kit, and their applications for the simultaneous detection of Aleutian virus and Canine Distemper Virus Asia 6 (Asia 6) in red pandas, belonging to the field of endangered wildlife virus detection technology. This invention provides a primer set and reagent kit that specifically identifies Aleutian virus and Canine Distemper Virus Asia 6 in red pandas. Detection can be completed within 20 minutes under constant temperature conditions of 39℃; it does not cross-react with other common pathogens and does not produce false positives due to primer dimer formation. In other words, it features rapid detection, high specificity, and high sensitivity. The implementation process is simple and easy to operate, requiring no specialized testing personnel for on-site testing. The detection is rapid and accurate, and initial applications have shown good results, providing a method for rapid diagnosis, virus tracing, wildlife disease monitoring, and early warning in zoos and wildlife rescue institutions.

The application discloses a method for detecting viruses and virus replication in media based on Small-RNA high-throughput sequencing. The application belongs to the technical field of virus detection, and the method for identifying virus types in a to-be-detected sample by Small-RNA sequencing developed by the application comprises the following steps: 1) extracting total RNA from a virus-infected to-be-detected sample, constructing a cDNA library, and separating small RNA sequence fragments from the cDNA library; 2) downloading virus sequences and classification databases from GenBank, and comparing the small RNA sequence fragments with virus sequence files; 3) comparing a contigs3 file with a virus nucleic acid database, selecting sequences with the minimum e value in the comparison results in the contigs3 file, calculating the coverage of the contigs3 sequences in corresponding virus genomes, distinguishing virus information with a coverage threshold, and deducing the virus source of the small RNA sequence.

The invention belongs to the technical field of plant virus molecular detection, particularly discloses a multiplex RT-PCR method and a kit for detecting important blueberry viruses, and particularly provides a multiplex RT-PCR detection primer for the blueberry viruses, and the sequence of the primer is shown as SEQ ID NO.1-12. The technical scheme provided by the invention is wide in detection range, high in specificity, high in specificity and high in sensitivity. The kit can be used for simultaneously detecting blueberry scorching virus, blueberry shock virus standard strains and variants thereof, tomato fruit ring spot virus, blueberry necrotic mottle virus and apple mosaic virus, solves the problem that the variants and different virus strains cannot be detected by a traditional ELISA method, covers main blueberry viruses and trans-boundary infected key virus types, and is high in specificity and high in sensitivity. The sensitivity is high.

The application relates to a method for generating a target AAV9 antibody epitope affinity peptide ligand by using a protein language model and a screening method and application thereof, and relates to an affinity peptide candidate sequence construction and screening method, an affinity peptide ligand, an affinity medium and application thereof for targeting adeno-associated virus type 9 (AAV9). The AAV9 capsid region recognized by Fab2-4 is taken as a target, structure analysis, natural amino acid positioning, key residue extraction and affinity peptide template construction are carried out, a candidate peptide library is generated by using a protein language model, and a candidate affinity peptide ligand is screened by combining molecular docking, binding free energy evaluation, refined docking and molecular dynamics simulation. The affinity peptide ligand is any one of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3 or SEQ ID NO. 4, can be immobilized on a solid phase matrix to form an affinity medium, is used for AAV9 recognition, capture and purification, and provides a technical scheme for AAV9 purification process development.

The application discloses a foot-and-mouth disease virus type A ELISA antibody detection kit and a preparation method and application thereof. The application determines a sequence of a foot-and-mouth disease virus type A GH loop epitope polypeptide and a non-specific combination of a foot-and-mouth disease virus type O, effectively solves the non-specific combination problem through sequence reduction, obviously reduces the detection sensitivity of the type A antibody after the reduction, further improves the detection sensitivity through a form of a multi-antigen peptide to display the polypeptide sequence. Finally, the polypeptide epitope combination, the antigen coated plate containing the polypeptide epitope combination and the ELISA antibody detection kit are determined. The total coincidence rate of the kit for detecting foot-and-mouth disease inactivated vaccine immune antibodies and negative serum is as high as 95% or more, but the positive detection rate of the kit for detecting foot-and-mouth disease synthetic peptide vaccine immune antibodies is obviously improved, and the cross reaction of the kit for detecting foot-and-mouth disease virus type O vaccine and pig foot-and-mouth disease virus type O inactivated vaccine immune antibodies is effectively reduced.

The invention discloses a fluorescence immunoadsorption method for detecting an O-type foot-and-mouth disease virus VP1 protein antibody and application, and relates to the technical field of genetic engineering, and the method is characterized by comprising the following steps: S1, preparing O-type foot-and-mouth disease virus VP1 protein by using a prokaryotic expression system; s2, preparing an anti-O type foot-and-mouth disease VP1 protein monoclonal antibody capable of recognizing a VP1 protein epitope; s3, coupling quantum dots with the anti-O-type foot-and-mouth disease VP1 protein monoclonal antibody to obtain a novel fluorescent probe; and S4, quantitatively detecting the O-type foot-and-mouth disease virus antibody through a fluorescence immunoadsorption method. According to the method, quantum dots and an O-type foot-and-mouth disease VP1 protein resisting monoclonal antibody are coupled to serve as a novel fluorescent probe, a fluorescence immunoassay method is established and used for quantitative detection of the O-type foot-and-mouth disease virus antibody, and compared with a traditional indirect competitive enzyme-linked immunosorbent assay, the method is more sensitive.

This application relates to the field of virus detection technology, specifically disclosing a dual-identification fluorescent RT-PCR detection kit for foot-and-mouth disease virus (FMD) types A and O. The kit includes a specific primer set, specific fluorescent probes, a fluorescent RT-PCR reaction solution, a positive control, and a negative control. The primer set contains primer pairs targeting the type A VP1 gene and the type O 3D gene, and includes degenerate bases to improve the detection coverage of variant strains. The fluorescent probe sequences are labeled with FAM and HEX reporter groups, respectively. Recombinant cold shock protein and recombinant human serum albumin are added to the reaction solution to optimize reaction performance. The kit also includes an internal standard system to monitor for false negatives. This kit solves the problems of insufficient broad-spectrum detection, multiple detection interference, and lack of quality control in existing technologies, and has the advantages of high specificity, high sensitivity, high reliability, and high throughput, making it suitable for rapid typing and monitoring of FMD virus.

Described is a recombinant vaccine against porcine epidemic diarrhea (PED) comprising a viral vector having inserted therein an exogenous nucleotide sequence encoding an antigen site of PED virus type 2, and a pharmaceutically acceptable solvent, adjuvant and / or excipient wherein the vaccine is suitable for generating an immune response in a porcine body, the vaccine does not need to be combined with other vaccines or forms of PED virus type 2.

The invention relates to the technical field of virus propagation path analysis, and discloses a virus propagation path analysis system and method based on big data, and the system comprises a propagation collection module and an intelligent analysis module. According to the system, management data of all municipal areas, management data of virus infected patients and all environmental factors affecting virus propagation are acquired through a propagation acquisition module and classified to form a data set, an intelligent analysis module sets a monitoring period, prevalence indexes are generated for the municipal areas where zero patients appear, and the prevalence indexes are analyzed. According to the method, the transmission range, the harm degree and the comprehensive condition of environment influence of viruses in jurisdictions are visually reflected, the multi-dimensional assessment precision is high, an intelligent analysis module focuses on transmission paths of three dimensions of population flow, material circulation and medical resources, the virus transmission risk degree between adjacent jurisdictions is assessed, risk indexes are generated, and the risk indexes are analyzed. Corresponding virus propagation prevention and control suggestions are output according to different virus types, a scientific prevention and control strategy which is graded according to popularity and applied according to propagation risks is established, and the intelligent prevention and control effect is good.

The invention provides a tobacco virus classification model construction method based on a tobacco leaf hyperspectrum, and belongs to the technical field of tobacco virus classification models. Performing spectrum topological manifold embedding on the enhanced spectrum data to calculate a persistent homology characteristic value and a Betti number sequence value, performing kernel principal component analysis dimension reduction to obtain a topological embedding characteristic vector, and outputting a fusion characteristic vector through a multi-mode attention fusion module by combining thermal infrared temperature field data and chlorophyll fluorescence dynamic curve data; performing data enhancement on a minority class of samples by utilizing a spectrum generative adversarial network, inputting a fusion feature vector into a virus classification and identification model adopting a focus loss function and a dynamic network adjustment mechanism for training, and realizing virus type classification and disease severity grade identification of the tobacco leaves to be detected. The problem that different types of tobacco viruses are difficult to accurately identify and distinguish in the early stage of diseases is solved.

A recombinant herpes simplex virus type 1 (HSV-1), a recombinant HSV-1 vector, and a method of preparing the recombinant HSV-1, may enable easy genetic modification, as a relatively large foreign gene may be inserted or various foreign genes may be inserted simultaneously, as ICP6 and IR regions are simultaneously deleted. These may be used in cancer treatment as an oncolytic virus that is safe, while having an excellent effect in killing cancer cells.

Disclosed herein is a composition of a compound that has superior pharmacokinetics and pharmacodynamics as compared to conventional interferon. In addition, a method to use such composition to treat human T-cell leukemia virus type 1 (HTLV-1) associated diseases are also disclosed.

The present invention discloses a kit and a detection kit for detecting herpes simplex virus in tears, the kit comprises: a housing, which comprises an internal cavity, and a sample application area and a detection window which are respectively communicated with the internal cavity; the immunochromatography test strip comprises a sample pad, a release pad and a nitrocellulose membrane which are connected in sequence, the immunochromatography test strip is arranged in the inner containing cavity, and part or all of the sample pad extends to the sample application area; the sampling assembly is in pivot connection with the shell and limits a first limit position, and when the sampling assembly is located at the first limit position, the sampling assembly is in surface contact with the sample pad; wherein herpes simplex virus type 1 G glycoprotein marked by fluorescence is embedded on the release pad; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is coated with an anti-human IgA antibody, the quality control line is coated with a goat anti-rabbit IgG antibody, and the detection line and the quality control line are exposed to the detection window.

The invention discloses a multiple RT-qPCR detection method for six chicken vertical transmission viruses and application, and belongs to the technical field of virus detection. The method comprises the following steps: designing specific primers and probes according to gene conserved regions of six viruses, namely CAV, ALV-J, REV, ARV-I, FAdV-4 and CAstV; single TaqMan RT-qPCR and multiple TaqMan RT-qPCR are sequentially established, a reaction system is optimized, reaction conditions are determined, the multiple TaqMan RT-qPCR is good in specificity and high in sensitivity, and the lowest detection rate of 102 copies / mu L can be achieved; when the kit is used for clinical sample detection, the virus type can be accurately judged, the detection result is consistent with that of a dye-method single RT-qPCR, and the kit has higher sensitivity. The method is suitable for early-stage rapid detection and differential diagnosis of mixed infection of multiple pathogens in breeding hens, overcomes the defects that a conventional method is complex in detection system and operation, time-consuming, labor-consuming and poor in sensitivity, and has a good application prospect.

The application discloses a feline panleukopenia virus, feline herpes virus and feline calicivirus yolk antibody preparation and a preparation method and application thereof. The feline triple yolk antibody preparation comprises a core material, and the core material comprises feline calicivirus yolk antibody, feline panleukopenia virus yolk antibody, feline herpes virus type 1 yolk antibody and probiotics. The application can produce specific neutralization to feline calicivirus, feline panleukopenia virus and feline herpes virus type 1, significantly reduces the infection rate and the severity of clinical symptoms, is stably released in the gastrointestinal tract after oral administration, the antibody activity retention rate is more than 90%, the probiotics synergistically regulate intestinal microecology, enhance the immune response of the body and improve the overall protection efficiency.

To provide a means for treating HTLV-1-associated myelopathy (HAM / TSP) and HTLV-1 infectious diseases by identifying important molecules on the side of infected cells and on the side of non-infected cells in the cell-cell contact infection of human T cell keukemia-1 virus (HTLV-1) and inhibiting the HTLV-1 cell-cell contact infection by inhibiting the mutual action thereof.SOLUTION: The HTLV-1 cell-cell contact infection inhibitor contains a substance inhibiting the interaction between N-acetyllactosamine (LacNAc) and galectin-3 (Gal-3).SELECTED DRAWING: None

A synergistic botanical formulation and related methods for the treatment of herpes virus infections, particularly herpes simplex virus type 1 (HSV-1), are disclosed. The formulation comprises at least one botanical extract with an extracellular antiviral mechanism and at least one botanical extract with an intracellular antiviral mechanism, selected through in vitro assays to provide complementary modes of action. Extracts are prepared under conditions that selectively isolate hydrophilic or hydrophobic constituents, and are combined in proportions based on individual antiviral activity to maximize synergistic inhibition of viral replication. The formulation is standardized to measured biological activity rather than marker compounds, ensuring consistent therapeutic performance despite variability in botanical sources. When administered topically, the formulation reduces lesion healing time, increases antiviral potency compared to conventional over-the-counter treatments, and decreases the likelihood of viral resistance development. Pharmaceutical compositions, methods of preparation, and treatment protocols are also provided.

The invention discloses a method for detecting beta-glucuronidase to evaluate the ability of shrimps to resist a decapod iridovirus type 1, and belongs to the technical field of biological breeding of aquatic animals. The method comprises the following steps: detecting beta-glucuronidase in a sample by qPCR (quantitative polymerase chain reaction) to obtain a 2-delta delta Ct value of the beta-glucuronidase, and evaluating the anti-decapod iridovirus type 1 capability of the shrimps according to the 2-delta delta Ct value, when the 2-delta delta Ct is less than or equal to 0.15, the resistance is evaluated to be high, when the 2-delta delta Ct is more than 0.15 and less than 0.45, the resistance is evaluated to be medium, and when the 2-delta delta Ct is more than or equal to 0.45, the resistance is evaluated to be susceptible. And through experimental verification, the prediction accuracy of unknown sample verification is more than 86.7%, and the AUC area of the ROC curve is 0.8864. In conclusion, the invention provides a standardized and operable evaluation method for macrobrachium rosenbergii disease-resistant molecular breeding.

The invention discloses a virus-mediated gene manipulation method for an embedded in-vitro follicle culture system and application, and belongs to the technical field of biology. According to the virus-mediated gene manipulation method for the embedded in-vitro follicle culture system disclosed by the invention, the optimal virus type is screened and the titer and chemical enhancement conditions are optimized, so that the follicle growth, ovulation, oocyte maturation, fertilization, hormone secretion and other core functions are not influenced; efficient, mild and controllable gene expression regulation is realized; a PCR amplification detection means proves that the vector is not integrated into an oocyte genome under the research condition and is not transferred to a progeny through a reproductive line. The method provides a safe, standardized and reproducible technical route for ovarian biological mechanism research, in-vitro disease model construction and drug / poison evaluation.

The application relates to the technical field of deep learning, and discloses a 6-HB targeting membrane fusion inhibition peptide prediction method, device, equipment and medium, which comprises the following steps: generating a 6-HB targeting membrane fusion inhibition candidate peptide; inputting the membrane fusion inhibition candidate peptide into a pre-constructed 6-HB targeting membrane fusion inhibition peptide prediction model for classification prediction to obtain the classification result of the membrane fusion inhibition candidate peptide; the membrane fusion inhibition peptide prediction model is a two-stage transfer learning classification model, the first stage is based on sequence general features to perform binary classification prediction of antiviral peptides and non-antiviral peptides; the second stage is combined with spatial conformation features to perform binary classification prediction of membrane fusion inhibition peptides and non-membrane fusion inhibition antiviral peptides; and the classification result is screened to obtain the 6-HB targeting membrane fusion inhibition candidate peptide. The method realizes the leap from coarse-grained antiviral activity recognition to fine-grained 6-HB inhibition activity classification, and improves the prediction accuracy of the 6-HB inhibition peptide specific to the envelope virus type / subtype.

The application provides an attenuated, replication-controllable forward HSV tracing system and a construction method and application thereof. Specifically disclosed is a recombinant herpes simplex virus type 1 crossing a nerve synapse, which is obtained by knocking out two copies of a nerve virulence factor coding gene gamma 34.5 in a H129 strain genome, then back-supplementing an expression-controllable gamma 34.5 self-degradation recombination gene into the original gene site of gamma 34.5, and the expression-controllable gamma 34.5 self-degradation recombination gene contains the gamma 34.5 gene with a function domain participating in the cell autophagy process knocked out and a degradation sequence PEST. The herpes simplex virus provided by the application can be used as a gene transduction carrier for long-term high expression of a target gene, long-term, anterograde crossing multi-level synapse structure tracing of a nerve circuit, functional nerve circuit analysis and the like, and has wide application value in nervous system targeted gene therapy, oncolytic virus therapy, virus replication and pathogenic mechanism analysis, animal infection model establishment and the like.

This invention discloses a recombinant herpes simplex virus vector expressing a HER2-deficient tyrosine kinase domain and its anti-tumor application, belonging to the fields of genetic engineering and tumor therapy. The vector is constructed via CRISPR / Cas9-mediated homologous recombination. After co-transfecting 293T cells with the pX459-γ34.5-KO vector and a homologous recombination target fragment expressing a HER2-deficient tyrosine kinase domain sequence, wild-type herpes simplex virus type 1 infection-mediated recombination is used to achieve targeted replacement of the viral γ34.5 site with the target sequence. This vector can efficiently infect HER2-negative / low-expressing tumor cells and enhance the killing effect of HER2-targeting antibody-drug conjugates (T-DM1 or T-DXd). In vitro and in vivo experiments have confirmed that it has significant anti-tumor activity, either alone or in combination with HER2-targeting antibody-drug conjugates, with no significant damage to vital organs and good safety, providing a new strategy for the treatment of related tumors.