92 results about "Virus types" patented technology

The invention discloses a reagent and a method for detecting berberine-resistant viruses. The reagent provided by the invention comprises an antibody or an antigen binding fragment thereof, the antibody or the antigen binding fragment thereof comprises a heavy chain complementarity determining region HCDR1-3 and / or a light chain complementarity determining region LCDR1-3, the heavy chain complementarity determining region HCDR1-3 has a sequence as shown in SEQ ID NO.5-7, and the light chain complementarity determining region LCDR1-3 has a sequence as shown in SEQ ID NO.8-10. The reagent can be used for detecting the berberine-resistant herpes simplex virus type 1, so that the treatment effect can be evaluated in advance before berberine is applied for treatment.

The invention provides an animal vaccination risk prediction method and system based on a cross-modal large model, and relates to the technical field of vaccination risk prediction. Comprising a multi-modal data acquisition and integration module, a cross-modal feature extraction and semantic alignment module, a pre-training model construction and transfer learning module, a risk prediction and decision support module and a model verification and iterative optimization module. Misjudgment caused by same surface symptoms can be effectively avoided, in animal epidemic disease diagnosis, similar symptoms may be caused by different viruses, a traditional method is easily interfered by surface phenomena, and the optimized method can be used for diagnosis of animal epidemic diseases by constructing a causal diagram, analyzing real causes of symptoms and stripping confounding factors such as environment and physiological status. Through the means, the model can more accurately distinguish virus types, the misjudgment risk is remarkably reduced, and a reliable basis is provided for a subsequent vaccination strategy.

The application provides a tobacco virus classification model construction method based on tobacco leaf hyperspectrum, and belongs to the technical field of tobacco virus classification models. Fractional differential spectrum enhancement processing is adopted, persistent homology eigenvalues and Betti number sequence values are calculated through spectrum topological manifold embedding of enhanced spectrum data, dimension reduction is performed through kernel principal component analysis to obtain topological embedding feature vectors, fusion feature vectors are output through a multimodal attention fusion module in combination with thermal infrared temperature field data and chlorophyll fluorescence kinetic curve data, data enhancement is performed on minority class samples by using a spectral generative adversarial network, the fusion feature vectors are input into a virus classification and recognition model adopting a focal loss function and a dynamic network adjustment mechanism for training, virus type classification and disease severity grade recognition of tobacco leaves to be tested are realized, and the problem that different types of tobacco viruses cannot be accurately recognized and distinguished in the early stage of disease is solved.

The invention discloses a neutralizing swine monoclonal antibody pO18-40 and a neutralizing swine monoclonal antibody pO18-43 for foot and mouth disease virus type O. The amino acid sequences of a heavy chain variable region (VH) and a light chain variable region (VL) of the antibody pO18-40 are respectively as shown in SEQ ID No. 1 and SEQ ID No. 2; the amino acid sequences of VH and VL of the antibody pO18-43 are respectively as shown in SEQ ID No. 3 and SEQ ID No. 4. The antibody obtained by the invention is a full-swine-source antibody, can specifically neutralize the classical strain of the O-type foot-and-mouth disease virus, and can clearly distinguish the classical strain of the O / Cathay topological type from the variant strain of the O / Cathay topological type. A key antigen epitope recognized by the antibody is located at the 149th amino acid of a VP1 protein G-H ring, and the site is a key site of O / Cathay strain antigen variation and vaccine immune protection. The antibody provided by the invention provides an important tool and theoretical basis for serological detection of O-type FMDV, vaccine immune effect evaluation and optimal design of broad-spectrum vaccines.

Provided herein, inter alia, are compositions for treating Human T-cell lymphotropic virus type 1 (HTLV-1) associated diseases. The compositions include a protein having a zinc finger domain capable of binding a sequence within an HTLV-1 long terminal repeat (LTR). Further provided are methods of treating HTLV-1 associated diseases in a subject in need thereof. The methods include administering to the subject the protein including the zinc finger domain, or a nucleic acid encoding the protein.

The virus of the present disclosure is a multiple mutant virus in which two or more genes of herpes simplex virus type 2 (HSV-2) are altered and the alteration of the genes is impaired or reduced in gene function.

The present invention discloses an information processing method, device and equipment for respiratory syncytial virus typing, which relates to the technical field of virus classification. The present invention innovatively proposes an information processing method for respiratory syncytial virus typing. On the basis of the original one-hot encoding and zero-padding strategies, a hierarchical one-hot encoding strategy is innovatively proposed, which reduces the interference of the traditional zero-padding method on one-hot encoding and enhances the robustness to insertion mutations. Based on the hierarchical one-hot encoding strategy and neural network image classification technology, it can help doctors and scientific researchers make accurate diagnoses and treatment guidance, improve work efficiency, and help improve the efficiency and diagnosis and treatment quality of the medical and public safety industries.

The invention relates to multiple preparation processes for targeted treatment of a class of non-enveloped virus nucleic acid or early-stage difficult-to-diagnose epidemic situation control. The key point is that different epidemic situations caused by enveloped or non-enveloped viruses are summarized and recognized from thousands of viruses and epidemic situations which are erupted all the time; the light is adopted to activate the methylene blue riboflavin, so that a plurality of enveloped viruses and a plurality of non-enveloped viruses can be respectively inactivated; the light-activated methylene blue riboflavin can be embedded into a virus nucleic acid chain to prevent replication so as to achieve targeted therapy, and the light-activated methylene blue riboflavin compound preparation is firstly used for treatment when virus types are difficult to diagnose in the early stage of an epidemic situation. In order to control epidemic situation propagation as soon as possible, after it is confirmed that enveloped virus infection or non-enveloped virus infection exists, light-activated methylene blue and a light-activated riboflavin preparation are adopted for targeted therapy. The light-activated methylene blue is adopted for treating three or five positive cases of hepatitis B once every two days by 120 milligrams every time, and all the cases are effectively turned to negative. The preparation process comprises the step of preparing the photoactivated methylene blue riboflavin and the capsule of the photoactivated methylene blue riboflavin.

The invention relates to the technical field of pathogen detection, in particular to a primer probe group for detecting herpes virus type 1-8 as well as a freeze-drying agent and application of the primer probe group. The primer probe group provided by the invention can be used for performing multiplex PCR detection on the herpes virus type 1-8, has good reagent specificity, has no nonspecific cross reaction with pathogens except the herpes virus type 1-8, has high sensitivity, has the lowest detection limit of 200 copies / mL, and provides a timely, effective, simple and convenient auxiliary detection tool for clinical detection. Furthermore, a freeze-drying agent containing the primer probe group provided by the technical scheme is good in stability, can be stored at normal temperature (1 year) and transported, has intra-group and inter-group variation coefficients of less than 3% when being used for simultaneously detecting the herpes virus type 1-8, is good in repeatability, is a stable, efficient and simple herpes virus type 1-8 detection reagent, and has wide application prospects. The clinical application is convenient.

The invention discloses a yellow dwarf disease resistance related gene MS1 and application thereof, and belongs to the technical field of biology. The invention discloses for the first time that the MS gene is a core positive regulation factor of plant broad-spectrum antiviral property through systematic research. Experiments prove that replication and diffusion of various pathogens such as BYDV, BSMV, TRV, PVX and BSCTV can be remarkably inhibited by up-regulating MS expression of plants such as wheat, barley and tobacco, and resistance does not depend on virus types. Mechanism research shows that MS silencing is achieved by enhancing PTGS-mediated RNA silencing and other basic resistance. The discovery breaks through the traditional mindset of'enhancing resistance genes', and a brand new strategy of'removing resistance inhibition factors' is created. The invention proves that MS inhibition can realize multiple virus resistance and does not damage growth and development.

Described herein are compositions and methods for reducing or eliminating latent herpes simplex virus type 2 (HSV-2) in HSV-2 infected cells, or for reducing or eliminating latent HSV-2 reactivation in HSV-2 infected cells, thereby providing viable therapeutic methods for latent HSV-2 infection. The compositions comprise a plurality of viral vectors, where the viral vectors comprise a sequence encoding a HSV-2 specific megasuclease. The method comprises delivering a plurality of one or more viral vectors to the HSV-2 infected cell, where each of the one or more viral vectors comprises a sequence encoding an HSV-2 specific megasuclease.

Described herein are systems and methods that may be used to differentially detect viral serotype specific nucleic acid. For example, these systems may comprise multiple DNA-nanostructures, capture oligonucleotides and protector oligonucleotides, wherein each DNA-nanostructure and its associated capture oligonucleotide and protector oligonucleotide are specific for a unique viral type or serotype.

The application provides a fusion protein, a rabbit hemorrhagic disease virus type 2 self-assembled nanoparticle vaccine and application, and belongs to the technical field of biological medicine. The application provides a fusion protein, which comprises a first polypeptide, a hinge region of a rabbit hemorrhagic disease virus type 2 capsid protein, a P region of a rabbit hemorrhagic disease virus capsid protein and a second polypeptide connected in sequence; the first polypeptide and the second polypeptide are polypeptides containing four cysteines respectively. The fusion protein RGD4C-HP-RGD4C of the application can self-assemble to form nanoparticles, and the particle size is 40-50 nm. The fusion protein of the application can induce domestic rabbits to produce specific antibodies against RHDV2, produce a strong humoral immune response, and provide complete protection for the domestic rabbits.

The invention discloses a kit for detecting berberine-resistant viruses and application of the kit. The kit provided by the invention comprises an antibody or an antigen binding fragment thereof, a buffer agent and a signal generation system, the antibody or the antigen binding fragment thereof comprises a heavy chain complementarity determining region HCDR1-3 and a light chain complementarity determining region LCDR1-3, the sequences of the heavy chain complementarity determining region HCDR1-3 are respectively as shown in SEQ ID NO.5-7, the sequences of the light chain complementarity determining region LCDR1-3 are respectively as shown in SEQ ID NO.8-10, and the signal generation system comprises a buffer agent and a signal generation system. Or the sequences of the heavy chain complementarity determining region HCDR1-3 are respectively shown as SEQ ID NO.15-17, and the sequences of the light chain complementarity determining region LCDR1-3 are respectively shown as SEQ ID NO.18-20. The kit can be used for detecting the berberine-resistant herpes simplex virus type 1, so that the treatment effect can be evaluated in advance before berberine is applied for treatment.

The present invention relates to a multiplex gene detection system for viral and bacterial infection of the central nervous system, comprising forward and reverse primers for detecting enterovirus, human double echovirus, Mycobacterium tuberculosis, Listeria monocytogenes, Streptococcus pneumoniae, varicella zoster virus, mumps virus, cytomegalovirus, herpes simplex virus type 1, herpes simplex virus type 2, Neisseria meningitidis, Epstein-Barr virus, Streptococcus agalactiae, Haemophilus influenzae, human herpes virus type 6, Escherichia coli K1 and Japanese encephalitis virus, and the detection sample is cerebrospinal fluid. The multiplex gene detection product for viral and bacterial infection of the central nervous system of the present invention has high throughput, low cost, high sensitivity and good specificity, and is used for synchronous identification and semi-quantitative analysis of multiple viral and bacterial infections of the central nervous system.

The invention provides a primer probe group and a kit for detecting an Acaspot virus, an Oya virus and a Peaton virus based on triple RT-qPCR (Reverse Transcription-Quantitative Polymerase Chain Reaction), and belongs to the technical field of molecular biology and nucleic acid detection. According to the present invention, by designing the primers and the probes for the Acaspot virus S fragment, the Oya virus S fragment and the Peaton virus M fragment, the Ct value is determined through the RT-qPCR method, and the existence of the virus nucleic acid and the virus type are determined according to the determination standard, such that the method has advantages of high sensitivity, good specificity, low detection cost, convenience, rapidness and the like, and is suitable for promotion and application in actual detection.

The invention belongs to the technical field of vaccine preparation, and discloses a mutant I-type herpes simplex virus subunit vaccine and a preparation method and application thereof, herpes simplex virus gB protein is gB protein or gB H516P mutant protein, the amino acid sequence of the gB protein is shown as SEQ ID NO.1, and the amino acid sequence of the gB H516P mutant protein is shown as SEQ ID NO.2; and the composite adjuvant is composed of CpG oligodeoxynucleotide and QS-21. Comparison experiments prove that humoral immune response and cellular immune response levels induced by the subunit vaccine combined by the CpG oligodeoxynucleotide and QS-21 composite adjuvant are equivalent to immune response levels induced by mRNA vaccines, and the neutralizing effect of serum of mice inoculated with the subunit vaccine is also equivalent to that of serum of mRNA group mice. And a foundation is laid for popularization of subunit vaccines.

The invention relates to the technical field of virus detection, and particularly discloses a dual-identification fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection kit for foot-and-mouth disease viruses A and O. The kit comprises a specific primer combination, a specific fluorescent probe, a fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) reaction solution, a positive control and a negative control, the primer combination comprises a primer pair targeting an A type VP1 gene and an O type 3D gene, and a degenerate base exists in the primer pair so as to improve the detection coverage rate of variant strains; fAM and HEX reporter groups are respectively marked by fluorescent probe sequences; the recombinant cold shock protein and the recombinant human serum albumin are added into the reaction liquid to optimize the reaction performance; the kit also comprises an internal standard system for monitoring false negative; the kit solves the problems of insufficient broad spectrum, multiple detection interference and quality control deficiency in the prior art, has the advantages of high specificity, high sensitivity, high reliability and high throughput, and is suitable for rapid typing and monitoring of foot and mouth disease viruses.

The invention belongs to the technical field of veterinary biology, and particularly relates to a swine foot and mouth disease virus type A immune peptide fusion protein as well as a preparation method and application thereof. The invention provides a swine foot-and-mouth disease virus type A immune peptide fusion protein. The swine foot-and-mouth disease virus type A immune peptide fusion protein has an amino acid sequence as shown in SEQ ID NO: 1. Compared with an epitope predicted in the prior art, the recombinant immune composite peptide provided by the invention has better immunogenicity, can obtain a neutralizing antibody with higher titer, and has better animal protection capacity.

The invention provides a fusion protein, a rabbit hemorrhagic disease virus type 2 self-assembled nanoparticle vaccine and application, and belongs to the technical field of biological medicine. The invention provides a fusion protein. The fusion protein comprises a first polypeptide, a hinge region of a rabbit hemorrhagic disease virus type 2 capsid protein, a P region of the rabbit hemorrhagic disease virus capsid protein and a second polypeptide which are connected in sequence, the first polypeptide and the second polypeptide are respectively polypeptide containing four cysteines. The fusion protein RGD4C-HP-RGD4C disclosed by the invention can be self-assembled to form nano particles, and the particle size is 40 to 50 nm. The fusion protein provided by the invention can induce a rabbit to generate a specific antibody aiming at RHDV2, generate a relatively strong humoral immune response and provide complete protection for the rabbit.

The application provides a 1-substituted cinnamoyl-2-oxo lycorine compound, a preparation method and application thereof. The structure of the compound is shown in the following formula I: wherein the benzene ring has one or more R groups which are substituents on the benzene ring, and the R group is selected from hydrogen, C 1‑6 alkyl, C 1‑6 alkylalkoxy, halogen, halogenated C 1‑6 alkyl, halogenated C 1‑6 alkoxy, nitro and dioxo. The compound shows excellent antiviral effect, especially for herpes simplex virus type I (HSV-1), and the compound has better safety.

The invention discloses a porcine reproductive and respiratory syndrome virus typing quadruple fluorescent quantitative RT-PCR (reverse transcription-polymerase chain reaction) detection composition and a kit thereof. The detection composition is used for simultaneously detecting four viruses, namely PRRSV-2 (porcine reproductive and respiratory syndrome virus 2), NADC30-like PRRSV (porcine reproductive and respiratory syndrome virus), HP-PRRSV (porcine reproductive and respiratory syndrome virus) and NADC34-like PRRSV; the kit obtained on the basis of the detection composition has good sensitivity, specificity and repeatability, nucleic acids of PRRSV-2, NADC30-like, HP-PRRSV and NADC34-like PRRSV strains can be effectively distinguished, the virus types can be accurately and rapidly identified by analyzing fluorescent quantitative results, the detection period is greatly shortened, and the kit has the advantages of being easy to operate, high in sensitivity, rapid in detection and the like, and has a good application prospect in the detection of PRRSV-2, NADC30-like, HP-PRRSV and NADC34-like PRRSV strains. And a new tool is provided for monitoring the propagation of the PRRSV in the swinery.

The invention discloses a primer group and a kit for simultaneously detecting a panda aleutian virus and a panda canine distemper virus Asia type 6 and application of the primer group and the kit, and belongs to the technical field of virus detection of endangered wild animals. The invention provides a primer group and a kit for specifically recognizing the aleutian disease virus and the canine distemper virus Asia 6 type of the panda, and detection can be completed within 20 minutes under the condition of constant temperature of 39 DEG C; according to the present invention, the method has characteristics of rapid detection, good specificity, high sensitivity and the like, does not produce the cross reaction with other common pathogens, does not produce the false positive phenomenon due to the generation of the primer dimer, has characteristics of rapid detection, good specificity, high sensitivity and the like, and has characteristics of simple and easy operation, and on-site detection without special detection personnel; the method is rapid and accurate in detection, has a good application effect in preliminary application, and provides a method for rapid diagnosis, virus traceability, epidemic disease monitoring and early warning of a wild animal epidemic disease source and the like in zoos and wild animal rescue institutions.

The invention provides a molecular diagnosis method for avian leukosis virus and Marek's disease virus mixed infection. The molecular diagnosis method comprises the following steps: (S01) collecting diseased material tissues; (S02) carrying out nucleic acid extraction on the sick material tissue of the sick chicken; (S03) detecting the nucleic acid by using specific primers of avian leukosis and Marek's disease; (S04) preparing a PCR (Polymerase Chain Reaction) detection system, and respectively adding ALV-env gene amplification primers and MDV-meq gene amplification primers for gene amplification; (S05) carrying out 1% agarose gel electrophoresis detection on the amplification product in the step (4), and carrying out sequence determination after determining a target band; (S06) carrying out BLAST comparison on the sequences obtained by sequencing, carrying out gene sequence analysis and drawing an evolutionary tree; and (S07) determining the virus type according to the homology and the evolutionary branches, so as to realize rapid diagnosis of ALV and MDV mixed infection, strain characteristic analysis and exclusion of other similar epidemic diseases, and provide technical support for accurate prevention and control of AL and MD in the poultry industry, provenance purification and epidemiological investigation.

The invention relates to a pediatric respiratory tract infection rapid diagnosis device. By setting a clinical manifestation database of various children respiratory diseases, data can be filled into the database according to external clinical manifestations of various children under different respiratory viruses; and the specific child respiratory tract infection virus type is determined through preliminary clinical manifestation data symptom judgment and further saliva detection, so that the diagnosis rhythm is accelerated, and the diagnosis accuracy is improved.

The invention discloses a virus prediction and protection recommendation method and system based on a knowledge graph and related equipment. According to the scheme, firstly, data cleaning and preprocessing are carried out on historical virus data; constructing a virus knowledge graph based on the preprocessed historical virus data; then, based on the constructed virus knowledge graph, carrying out model training and learning by utilizing a DGL-KE framework to form a virus recommendation model embedded based on the oversized knowledge graph; and finally, calculating the input virus attribute parameters by a virus recommendation model which is formed by training and is embedded based on the super-large knowledge graph to generate the most possible virus type and the vulnerable equipment type. According to the scheme, by constructing the large-scale virus knowledge graph, not only can the differences of attributes and graph structures of different viruses be visually seen, but also the prediction results of the most possible virus type and the vulnerable equipment type can be calculated according to the input virus related information.

This invention discloses a primer set, reagent kit, and their applications for the simultaneous detection of Aleutian virus and Canine Distemper Virus Asia 6 (Asia 6) in red pandas, belonging to the field of endangered wildlife virus detection technology. This invention provides a primer set and reagent kit that specifically identifies Aleutian virus and Canine Distemper Virus Asia 6 in red pandas. Detection can be completed within 20 minutes under constant temperature conditions of 39℃; it does not cross-react with other common pathogens and does not produce false positives due to primer dimer formation. In other words, it features rapid detection, high specificity, and high sensitivity. The implementation process is simple and easy to operate, requiring no specialized testing personnel for on-site testing. The detection is rapid and accurate, and initial applications have shown good results, providing a method for rapid diagnosis, virus tracing, wildlife disease monitoring, and early warning in zoos and wildlife rescue institutions.

The application discloses a method for detecting viruses and virus replication in media based on Small-RNA high-throughput sequencing. The application belongs to the technical field of virus detection, and the method for identifying virus types in a to-be-detected sample by Small-RNA sequencing developed by the application comprises the following steps: 1) extracting total RNA from a virus-infected to-be-detected sample, constructing a cDNA library, and separating small RNA sequence fragments from the cDNA library; 2) downloading virus sequences and classification databases from GenBank, and comparing the small RNA sequence fragments with virus sequence files; 3) comparing a contigs3 file with a virus nucleic acid database, selecting sequences with the minimum e value in the comparison results in the contigs3 file, calculating the coverage of the contigs3 sequences in corresponding virus genomes, distinguishing virus information with a coverage threshold, and deducing the virus source of the small RNA sequence.

The invention belongs to the technical field of plant virus molecular detection, particularly discloses a multiplex RT-PCR method and a kit for detecting important blueberry viruses, and particularly provides a multiplex RT-PCR detection primer for the blueberry viruses, and the sequence of the primer is shown as SEQ ID NO.1-12. The technical scheme provided by the invention is wide in detection range, high in specificity, high in specificity and high in sensitivity. The kit can be used for simultaneously detecting blueberry scorching virus, blueberry shock virus standard strains and variants thereof, tomato fruit ring spot virus, blueberry necrotic mottle virus and apple mosaic virus, solves the problem that the variants and different virus strains cannot be detected by a traditional ELISA method, covers main blueberry viruses and trans-boundary infected key virus types, and is high in specificity and high in sensitivity. The sensitivity is high.

The application relates to a method for generating a target AAV9 antibody epitope affinity peptide ligand by using a protein language model and a screening method and application thereof, and relates to an affinity peptide candidate sequence construction and screening method, an affinity peptide ligand, an affinity medium and application thereof for targeting adeno-associated virus type 9 (AAV9). The AAV9 capsid region recognized by Fab2-4 is taken as a target, structure analysis, natural amino acid positioning, key residue extraction and affinity peptide template construction are carried out, a candidate peptide library is generated by using a protein language model, and a candidate affinity peptide ligand is screened by combining molecular docking, binding free energy evaluation, refined docking and molecular dynamics simulation. The affinity peptide ligand is any one of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3 or SEQ ID NO. 4, can be immobilized on a solid phase matrix to form an affinity medium, is used for AAV9 recognition, capture and purification, and provides a technical scheme for AAV9 purification process development.