The invention discloses an ultrasonic-assisted reservoir stratum chemical blockage removal experimental facility and an experimental method. The experimental facility comprises a rock core clamp holder, a ring compression feed device, an ultrasonic energy converter, an ultrasonic generator, a chemical reagent storage tank connected with a liquid inlet of the rock core clamp holder by a conveying pipeline, a water storage tank, an oil storage tank and a liquid container connected with a liquid outlet of the rock core clamp holder. The experimental method comprises the following steps of: 1, pretreating a tested rock core; 2, testing the initial permeability of the rock core through water drive; 3, polluting the rock core; 4, testing the permeability of the polluted rock core through the water drive; 5, performing an ultrasonic-assisted chemical reagent blockage removal experiment; 6, testing the permeability of the rock core subjected to the blockage removal through the water drive; 7,performing the ultrasonic-assisted chemical blockage removal experiment under the condition of ultrasonic oscillation with different frequencies and amplitudes; and 8, processing data. The ultrasonic-assisted reservoir stratum chemical blockage removal experimental facility has reasonable design, complete functions and high using effect, is easy and convenient to arrange and operate, and can realize the pulse fluctuation of the chemical blockage removal under the action of the ultrasonic oscillation.