Chemical luminescence reagent kit for detecting herpes simplex virus type II IgM antibody

Abstract

The invention provides a chemiluminescence immunoassay test kit and a preparing method thereof for detecting herpes simplex virus type II IgG antibody. The technical principle that the test kit adopts the capture method to detect herpes simplex virus type II IgG antibody is that: antihuman u link monoclonal antibody is absorbed into solid-phase carrier, and sample to be tested is added in, and then specific antigen and enzyme labeled antibody are added in after washing so as to form immune complex, finally chemiluminescence zymolyte liquid is added in, and the content of the IgM antibody can be obtained through measuring the chemiluminescence value. The enzyme is alkaline phosphatase, and the chemiluminescence zymolyte liquid contains 1,2-butyl dioxide derivatives. The test kit has the advantages that the operation is simple, the precision is better, the sample detecting result conforms the detecting result by the enzyme linked immunity analysis method.

Description

technical field [0001] The invention relates to the field of immunoanalysis medicine, in particular to a chemiluminescence kit for detecting herpes simplex virus type II IgM antibody and a preparation method thereof. Background technique [0002] Herpes simplex virus (HSV) is round in shape, the inner layer is a core composed of double-stranded linear DNA, and the outer layer is a three-layer shell structure. There are two serotypes of HSV, HSV-I infection accounts for 10%, often oral mucosa, upper body skin, lymph node enlargement, mainly causes upper body skin, mucous membrane or organ herpes, but rarely infects the fetus. HSV-II type is genital herpes, accounting for 90%, mainly causing genital herpes, anus and skin herpes below the waist, and direct sexual transmission accounts for the vast majority. The symptoms of HSV infection in pregnant women are mainly herpes, ulcers and pain in the cervix, vagina and external genitalia. The symptoms of neonatal infection in the b...

AI Technical Summary

Problems solved by technology

Although radioimmunoassay is highly sensitive and specific, it has the disadvantages of short validity period and radioactive contamination

Although the fluorescent immunoassay method has strong specificity, high sensitivity and fast speed, the problem of non-specific staining has not been completely solved, the objectivity of the result judgment is not enough, and the procedure is relatively complicated

Although enzyme-linked immunoassay has high sensitivity and specificity, it has disadvantages such as endogenous enzyme interference, most of the substrates are toxic or carcinogenic, and external factors such as scratches will also affect the determination of absorbance.

Images