51 results about "FLUORESCENT IMMUNOASSAY" patented technology

The utility model relates to the technical field of detection equipment, in particular to a fluorescence immunoassay analyzer. Comprising a machine body, a detection window is arranged on the front side of the machine body, a multi-channel reaction disc is arranged on one side of the detection window, a plurality of incubation grooves are formed in the front side of the multi-channel reaction disc, the multi-channel reaction disc comprises a plurality of independently arranged timing modules, buzzers and indicator lamps, and the buzzers and the indicator lamps are electrically connected with the timing modules. When the reaction time of the test card on a certain channel in the multi-channel reaction disc is over, the buzzer and the indicator light can give an alarm; the device can immediately detect a plurality of temperature-controlled test cards in sequence, and does not need to wait for the completion of the temperature control of the next test card, so that a large amount of time can be saved.

The invention relates to an anti-mouse IgG nano antibody and alkaline phosphatase fusion protein as well as a preparation method and application thereof. The anti-mouse IgG nano antibody and alkaline phosphatase fusion protein sequentially comprises an anti-mouse IgG nano antibody, a connecting peptide and shewanella alkaline phosphatase from an amino terminal to a carboxyl terminal. The fusion expression of the anti-mouse IgG nano antibody and shewanella alkaline phosphatase in escherichia coli is designed, so that various defects of a chemical labeling method can be avoided, a high-purity and high-specific activity enzyme-labeled antibody can be prepared, the preparation period of a second antibody can be shortened, the preparation process of the second antibody can be simplified, the stability of batches can be improved, and the dependence on experimental animals can be reduced. The prepared anti-mouse IgG nano antibody and alkaline phosphatase fusion protein can be applied to the field of immunodetection, such as a chemiluminescence immunoassay method, an enzyme-linked immunosorbent assay method, an enzymatic fluorescence immunoassay method, an immunoblotting method and the like.

The application belongs to the field of nanometer material in environmental monitoring application, and discloses a fluorescence immunoassay method for detecting ovalbumin concentration based on carbon quantum dots, which comprises the following steps: using L-glutamic acid as a precursor, one-step preparation of carbon quantum dots with amino groups by ultrasonic hydrothermal method; using a reduced graphene oxide method to prepare graphene sheets, and optimizing the graphene by modifying polydiallyldimethylammonium chloride to obtain graphene sheets which can have good fluorescence resonance energy transfer with the carbon quantum dots with amino groups; and coupling the prepared carbon quantum dots with amino groups with a monoclonal antibody. The application directly prepares carbon quantum dots with amino groups by one-step hydrothermal method, and the quantum dots can be directly coupled with a monoclonal antibody. The carbon quantum dots involved in the application avoid the aminoization step, and the linear range can reach 0.1 ng / mL-3000 ng / mL.

1. The name of this design product: Portable fluorescent immunoassay analyzer. 2. Purpose of this design product: used for fluorescent immunoassay detection. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1.

The invention discloses a handheld fluorescence immunoassay analyzer with a dustproof function, and aims to solve the problem that dust and stains easily enter an existing handheld fluorescence immunoassay analyzer from a test paper insertion hole, so that the accuracy of a detection result is influenced. The analyzer comprises an analyzer body and a test paper insertion hole formed in one end of the analyzer body, a dustproof assembly is arranged at the end, close to the outer side of the analyzer body, of the test paper insertion hole, the dustproof assembly comprises a dustproof plate, a driving part and a stirring part, and the dustproof plate can elastically ascend and descend in the test paper insertion hole. During use, the stirring piece is rotated to drive the driving piece to enable the dustproof plate to open the test paper insertion hole, fingers do not need to contact the dustproof plate, insertion of test paper cannot be hindered, and the test paper insertion hole is closed after detection is completed, so that dust is effectively prevented from entering the analyzer in the detection process and after detection is completed; the accuracy of a detection result is improved.

The invention relates to a nano antibody for specifically recognizing a tacrolimus-antibody compound and application of the nano antibody, and belongs to the technical field of biological detection. A variable region of the nano antibody has three complementary determining regions CDR1, CDR2 and CDR3, can specifically recognize a tacrolimus-antibody compound, and can be used for detecting drugs by a double-antibody sandwich method; the kit is widely suitable for various immunodetection technologies such as an enzyme-linked immunosorbent assay (ELISA), a chemiluminescence immunoassay (CLIA), an electrochemical luminescence immunoassay (ECLIA), a fluorescence immunoassay (FIA), an immunochromatography test strip method and the like. According to the method, the limitation of a traditional competition method is overcome, high-sensitivity, high-specificity and high-stability detection is realized, the method can be adapted to various detection platforms, and a portable pretreatment process without centrifugation can also be realized.

The invention discloses a fluorescence immunoassay analyzer test fixture and a test method thereof, and belongs to the technical field of medical instrument tests, the fluorescence immunoassay analyzer test fixture comprises a first assembly body and a second assembly body movably connected with the first assembly body; the first assembly body is of a plate-shaped structure, the second assembly body is of a hollow box-shaped structure with a top cover and without a bottom cover, and the first assembly body is matched with the second assembly body; the first assembly body is of a double-layer structure and comprises a bottom plate and a fixing plate arranged on the bottom plate. Wherein the fixing plate is provided with a plurality of fixing grooves and fixing columns which are matched with the fluorescence immunoassay analyzer; clamping grooves matched with the second assembly bodies are further formed in the four corners of the fixing plate. The fluorescence immunoassay analyzer is accurately fixed and positioned through detachable connection of the second assembly body and the first assembly body, and the technical effects of simplifying the test operation process and improving the test stability and safety are achieved.

The invention discloses a near-infrared fluorescence immunoassay analyzer, and relates to the field of biomedical engineering.The near-infrared fluorescence immunoassay analyzer comprises an analyzer body, a dome-type fluorescence collector is installed in an inner cavity of a sealing seat, a narrow-band light filter is installed at the bottom of the dome-type fluorescence collector, and a piezoelectric focusing lens set is installed at the bottom of a light source sliding block; a light source sliding block is arranged in the analyzer body, a laser range finder and an optical fiber beam splitter are respectively arranged on two sides of the light source sliding block, a double-layer reagent card base body is arranged in the center of the top of the analyzer body, a snakelike microfluidic channel is formed in the top of the double-layer reagent card base body, a shape memory alloy is arranged at the bottom of the double-layer reagent card base body, and a thermosensitive actuator array is arranged at the bottom of the shape memory alloy. According to the near-infrared fluorescence immunoassay analyzer, eight-path parallel scanning is combined with dynamic focusing, so that the single-sample full-index detection time can be greatly shortened, the near-infrared fluorescence immunoassay analyzer can be compatible with various complex biological samples, a chemical color developing agent is not needed, and the experiment cost and the environmental pollution risk are reduced.

The invention discloses a fluorescence immunodetection analyzer and a detection analysis method thereof. The analyzer comprises a sheath flow pool which is vertically arranged, red and green laser excitation light sources which are horizontally arranged on the two sides of the sheath flow pool in the X-axis direction, and double independent light paths which are arranged on the two sides of the sheath flow pool in the Y-axis direction. A red laser light path separates fluorescence into three paths of signals of 730nm, 670nm and residual fluorescence through a series-connected dichotomy mirror group; the green laser light path collects diffuse reflection light. The four paths of optical signals are converted into voltage signals by four detectors, temperature compensation units integrated in the detectors correct the signals in real time based on a pre-calibrated dark current-temperature relation curve, and temperature drift is suppressed. And the signal processing system calls a pre-established signal model library to carry out comprehensive calibration and synchronous analysis on multi-channel signals, so that accurate distinguishing and counting of different fluorescent microspheres are realized. According to the invention, the problems of large temperature influence and low detection efficiency in the prior art are solved, and the stability, accuracy and multi-channel consistency of detection are remarkably improved.

The utility model discloses an optical detection structure and portable fluorescent immunoassay appearance. Optical detection structure includes first seat body, light source structure, second seat body, optical component, and the first seat body is formed with the first light guide hole and is linked with the first light guide hole first light hole, and second seat body is connected with first seat body, and is seted up with the second light guide hole opposite first light guide hole, and the light source structure's luminous part is close to first light hole setting, and optical component is located in first light guide hole and second light guide hole. The utility model integrates light source structure and optical component in one, compared with common 45 degree two -way spectroscope adds filter piece scheme, and the optical part required is less, and the structure is more compact, and the volume of whole optical detection structure is smaller, can be better applicable to portable fluorescent immunoassay appearance, and moreover, assembling is more convenient, can better guarantee the accuracy of the light hole and optical component alignment on light source structure.

1. The name of the design product: fluorescent immunoassay analyzer (RealG-FL12 Pro). 2. The use of the design product: a medical testing device for detecting the analyte in human whole blood, serum and plasma samples, etc., using fluorescent immunoassay technology to excite and measure the fluorescent signal, thereby realizing rapid identification and quantitative analysis of pathogens, and providing important information for the diagnosis and treatment of infectious diseases. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The utility model discloses an electronic fluorescence test paper disease detection pen type sensor, which adopts a dichroscope, a purple optical filter and a red optical filter to form an optical path, and reduces the use of a lens group compared with an existing fluorescence immunochromatography instant detector. A control circuit module and a photoelectric detection module are integrated in the electronic fluorescence test paper disease detection pen type sensor, and the sensor has the advantages of being small in size, compact in structure and convenient to carry.

The invention discloses a single-molecule fluorescence immunoassay kit for detecting dry plasma spot GFAP protein and a preparation method, and belongs to the technical field of immunodetection. The kit provided by the invention comprises an anti-GFAP mouse monoclonal antibody coated magnetic bead reagent, a biotinylated anti-GFAP mouse monoclonal antibody reagent, a streptavidin-beta-galactosidase reagent, a resorufin beta-D-galactoside reagent, a dry plasma spot eluent, a GFAP sample diluent, a GFAP calibration product, a GFAP quality control product and dry plasma spot filter paper. The dry plasma spot eluent contains PEG (Polyethylene Glycol) 6000 with the concentration of 15g / L to 25g / L, NaCl with the concentration of 15g / L to 20g / L, HEPES with the concentration of 11g / L to 12g / L, Triton X-100 with the concentration of 10g / L to 12g / L, EDTA (Ethylene Diamine Tetraacetic Acid) with the concentration of 4.5 mmol / L to 5.5 mmol / L, EGTA (Ethylene Glycol Terephthalate) with the concentration of 1.5 g / L to 2.0 g / L and BSA (Bovine Serum Albumin) with the concentration of 1.2 g / L to 1.8 g / L, and the pH value is adjusted to 7.3 to 7.5 by NaOH; aiming at the bottleneck problem of trace GFAP protein detection in a dried plasma spot sample, the detection sensitivity, accuracy and filter paper applicability are improved by optimizing an eluent formula, diluent components and a detection system.

The invention discloses a kit for rapidly detecting sST2. The kit comprises a test strip, the test strip comprises a back plate, and a water absorption pad, an NC membrane, a fluorescent pad and a sample pad which are sequentially laid on the back plate, the fluorescent pad is coated with a second monoclonal antibody which is labeled by fluorescent microspheres and is combined with a C-terminal unique region of sST2; the NC membrane is provided with a detection strip and a quality control strip, the detection strip is coated with a first monoclonal antibody combined with a third immunoglobulin structural domain of sST2, and the quality control strip is coated with a second antibody for resisting a second monoclonal antibody; according to the kit, rapid detection of sST2 can be completed within 6 minutes, cross reaction between isomers (such as soluble ST2 and transmembrane type ST2) is effectively eliminated, the detection accuracy and sensitivity are improved, the kit can directly use a whole blood sample for detection, centrifugation is not needed, and the detection time is saved; and the method is suitable for a full-automatic fluorescence immunoassay analyzer, and can realize automatic, convenient and rapid interpretation.

1. The name of the design product: fluorescent immunoassay analyzer (RealG-Fluo-2000). 2. The use of the design product: for detecting the measured object in human whole blood, serum and plasma samples, etc. by detecting the measured object in human whole blood, serum and plasma samples, etc. by using fluorescent immunoassay technology to excite and measure the fluorescent signal, so as to realize the rapid identification and quantitative analysis of pathogen. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The utility model provides a fluorescence immunoassay detector device which comprises a detector and a fluorescent reagent strip, and the detector is used for detecting the fluorescent reagent strip; a tooth-shaped structure is arranged at the bottom of the fluorescent reagent strip; the detector comprises an objective table, a reagent strip insertion opening, a detection device, a transmission gear and a clockwork spring device, wherein the detection device is provided with at least one detection site on the objective table; the reagent strip insertion opening allows the fluorescent reagent strip to enter or come out, the transmission gear is rotationally arranged on the objective table and is meshed with a tooth-shaped structure at the bottom of the fluorescent reagent strip, and the clockwork spring device drives the transmission gear to rotate; and the transmission gear rotates to drive the fluorescent reagent strip to enter the detection site or be separated from the detection site from the reagent strip insertion opening. According to the fluorescent reagent strip detection device, the damping spring structure is adopted for transmission for scanning detection of the fluorescent reagent strip, the electric quantity required by the whole detection device is small, the cost is greatly reduced, and the fluorescent reagent strip detection device is more suitable for household detection scenes.

The application discloses a reagent card bearing mechanism of a fluorescent immunoassay device, which comprises a support for providing basic support, a bearing plate rotationally connected with the support, the bearing plate comprising a main body part and a plurality of clamping parts arranged along the center of the main body part in a circumferential array, air passages being arranged between the clamping parts and the main body part, one end of each air passage being connected in communication with the center of the main body part, and adjacent two clamping parts and the main body part forming a card placement area of a reagent card; and a temperature control assembly comprising a heating plate, a temperature sensor, a fan and a control unit, the control unit controlling the working conditions of the heating plate and the fan according to the feedback of the temperature sensor, so that the card placement area is in a preset temperature interval. The reagent card bearing mechanism of the fluorescent immunoassay device has the beneficial effects of rapid temperature adjustment and excellent constant temperature effect.

The invention relates to the technical field of biological medicine detection, in particular to a method for detecting apramycin by using a quantum dot fluorescence immunoassay method. A sample and an apramycin monoclonal antibody solution are added into a coated elisa plate for incubation, then a fluorescent probe solution is subjected to incubation reaction, the fluorescence intensity is measured, and finally the apramycin content in the sample is calculated. CdSe / ZnS quantum dots and rabbit anti-mouse IgG are combined to serve as a novel fluorescent probe, a fluorescence immunoassay method is established and used for quantitative detection of apramycin in animal feed, and compared with a traditional indirect competitive enzyme-linked immunosorbent assay method, the fluorescence immunoassay method is more sensitive.

The utility model provides a full-automatic dry-type fluorescence immunoassay analyzer, which relates to the technical field of fluorescence immunoassay analyzers, and comprises a clamping and positioning mechanism and a feeding and discharging assembly, a fixedly inserted control observation part is arranged above the side of the clamping and positioning mechanism; a feeding and discharging assembly for bolt assembly is arranged above the side of the control observation component and comprises a lifting frame, a cross beam, a hydraulic cylinder, a push rod, a lifting block and a pneumatic clamp. A reagent can be continuously put into a detection position by controlling the observation component and the feeding and discharging component, and the driven gear can drive the assembly plate and the rotating column to rotate through meshing transmission operation of the driving gear and the driven gear, so that the lower empty disc and the upper disc rotate adaptively; therefore, the reagent can be effectively clamped in an increasing manner under the mutual cooperation of the hinged base and the clamping strip, so that the efficiency and quantity of single-time detection are improved.

A sample solution preparation method, a measurement unit, a measurement kit, and a sample solution preparation device for measuring a target compound contained in a food by using a fluorescent immunoassay method. The features of the present disclosure include a dialysis step for bringing a target compound-containing solution including a target compound into contact with a dialysate through a dialysis membrane to transfer the target compound to the dialysate, wherein the molecular weight cut-off of the dialysis membrane is in the range of 2 x 10 2 to 2 x 10 5 , and the volume of the dialysate is relatively smaller than the volume of the target compound-containing solution.

The application provides a glycated hemoglobin dissociation reagent and application thereof, and belongs to the technical field of biological detection. The dissociation reagent comprises 10-50 mM Tris buffer, 0.2-0.5 M ammonium chloride, 0.5%-1.0% (v / v) triton X-100, 0.2 wt%-1.0 wt% BSA and 0.02-0.2 M sodium chloride. When the dissociation reagent is used to dissociate a whole blood sample, the hemoglobin can be dissociated by mixing the dissociation reagent with the whole blood sample for 20-60 s, and a clear and transparent solution is obtained. The dissociation reagent can be used to prepare a kit for dissociating or / and detecting glycated hemoglobin. The glycated hemoglobin obtained by using the dissociation reagent can be directly used for fluorescence immunoassay, the interference of the color of hemoglobin itself on immunochromatographic detection is avoided, and the sensitivity is high and the repeatability is good.

The utility model belongs to the technical field of quantum dot fluorescence immunoassay analyzers, and particularly relates to a sample test tube conveying rack of a quantum dot fluorescence immunoassay analyser, which comprises a mounting plate mounted on an analyzer body, a first magnetic plate arranged on the outer side of the mounting plate, a first sliding groove formed in the mounting plate, and a conveying assembly arranged in the first sliding groove, a threaded block is arranged on the conveying assembly, a second sliding groove is formed in the threaded block, an elastic assembly is arranged in the second sliding groove, a connecting block is installed on the elastic assembly, a test tube rack is installed on the connecting block, and a plurality of through holes allowing reagent tubes to enter are formed in the test tube rack; a second magnetic plate and a third magnetic plate which are connected with the connecting block are arranged on the outer side of each through hole, and the positions of the second magnetic plates and the positions of the third magnetic plates correspond to the positions of the first magnetic plates. According to the device, the reagent tube can be vibrated during conveying, a solution in the reagent tube is effectively prevented from precipitating, meanwhile, the cost is low, and subsequent maintenance is facilitated.

The invention provides a microfluidic fluorescence immunoassay chip and device for evaluating the risk of having an Alzheimer disease and application of the microfluidic fluorescence immunoassay chip and device, and belongs to the technical field of microfluidic fluorescence immunoassay chip preparation. The microfluidic fluorescence immunoassay chip provided by the invention comprises a chip body, the chip body is provided with a closed micro-channel for a sample to flow, and the micro-channel comprises a sample adding hole, a reaction bin, a capturing bin and a waste liquid pool which are communicated in sequence; the interior of the reaction bin is coated with a mixture of a fluorescent microsphere labeled antibody A beta 42, p-tau-181 and p-tau-217; according to the position of the reaction bin, the capture bin is sequentially coated with an A beta 42 paired antibody, a p-tau-181 paired antibody, a p-tau-217 paired antibody and a second antibody. The micro-fluidic fluorescence immunoassay chip provided by the invention can accurately evaluate the risk of an individual to be detected suffering from Alzheimer's disease or the risk of the individual to be detected suffering from AD-derived MCI.

The application discloses a multifunctional portable single-channel detection device, which comprises a shell, a detection port and an incubation port, a fluorescent immunoassay component, a colloidal gold detection component, a constant-temperature incubation box, a mobile positioning mechanism, a microprocessor and a touch display screen arranged in the shell; a reagent card can be placed into the constant-temperature incubation box through the incubation port; the reagent card can be transferred to the fluorescent immunoassay component or the colloidal gold detection component by the mobile positioning mechanism for detection through the detection port; the microprocessor is electrically connected with other components, and the touch display screen realizes man-machine interaction; the application has the characteristics of multifunction, miniaturization and easy portability, and has a simple structure and is easy to mass produce.

This invention discloses a monoclonal antibody against rabies virus N protein, a detection reagent, and their applications. The monoclonal antibody against rabies virus N protein includes a heavy chain variable region and a light chain variable region; the amino acid sequence of the heavy chain variable region is shown in SEQ ID No. 1; the amino acid sequence of the light chain variable region is shown in SEQ ID No. 2. This invention provides a monoclonal antibody with high affinity and detection sensitivity against rabies virus N protein, laying the foundation for the research and development and promotion of colloidal gold test strips and fluorescent immunoassay strips.

The utility model discloses a full-automatic fluorescence immunoassay analyzer which comprises an analyzer body, two placing cavities are symmetrically formed in the analyzer body, a switch closing assembly is arranged on the front face of each placing cavity, each switch closing assembly comprises two blocking doors symmetrically arranged on the front face of the analyzer body, and internal grooves are formed in the blocking doors; a sealing piece is mounted on the inner side of the internal groove, a sliding block is mounted on one side of the blocking door, the sliding block is mounted in a longitudinal sliding frame in a sliding manner, the longitudinal sliding frame is fixedly mounted on the analyzer body, a second screw rod is rotationally mounted in the longitudinal sliding frame, and the second screw rod is in threaded connection with the sliding block; in the working process, the blocking door is driven by the arranged second motor to move longitudinally, the blocking door makes contact with the surface of the analyzer body, on one hand, opening and closing of the containing cavity are convenient, test tubes are convenient to take and place, and on the other hand, due to the fact that the blocking door and the inner side sealing plate make contact with the surface of the analyzer body, the sealing effect on the containing cavity is improved.

The utility model discloses a fluorescence immunoassay reagent card detection device, which comprises a protective shell, a reagent card transmission inlet, a reagent card transmission outlet, a fluorescence immunoassay reagent card detection module, a fluorescence immunoassay reagent card detection module, a fluorescence immunoassay reagent card detection module and a fluorescence immunoassay reagent card detection module, the plurality of conveying channels are fixedly arranged in the protective shell, are used for conveying the reagent cards to be detected, are arranged in parallel, are positioned on the same horizontal plane, and are respectively communicated with the reagent card conveying inlet and the reagent card conveying outlet; the optical assembly is fixedly arranged in the protective shell, is positioned right above the plurality of conveying channels, and faces the detection areas of the conveying channels so as to perform fluorescence signal detection on the plurality of reagent cards at the same time; and the driving assemblies are arranged in the protective shell and are arranged in a one-to-one correspondence manner along the lower parts of the plurality of conveying channels so as to drive the reagent cards to move along the conveying channels.

The invention relates to the technical field of fluorescence immunoassay, in particular to a fluorescence immunoassay kit for detecting horse adrenocorticotropin and a preparation method of the fluorescence immunoassay kit. The kit comprises a sample diluent with specific components and a test strip, the test strip is provided with a pretreatment pad marker combination pad, and a fluorescent microsphere labeled antibody mixed solution sprayed on the test strip can be efficiently combined with a target substance. The pretreatment pad, the marker combination pad and a sample diluent have a synergistic effect, so that sample interference is effectively eliminated, the chromatography rate is regulated and controlled, the reaction microenvironment is stabilized, the activity loss of a target object is reduced, non-specific combination is realized, and an optimized preparation process is matched, so that the detection accuracy and reliability are remarkably improved, and the detection performance is excellent in a wide concentration range. Meanwhile, the standard preparation process ensures the product quality uniformity, is suitable for batch production, fills the blank in the related detection field, and has good practical application value and wide prospects.

This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a gold-plated solid surface bound with a fluorescent label, a light source, a light correcting lens, and an optical detector to detect the fluorescent emission light. The solid is made of plastic doped with glass fibers and the solid surface is coated with gold, and the hardness of the solid is at least 75 based on Rockwell M hardness scale. The system provides high sensitivity and low variability for fluorescent immunoassays.

The application belongs to the technical field of IVD detection, and particularly relates to a dry-type fluorescence immunoassay analyzer for IVD detection. The dry-type fluorescence immunoassay analyzer for IVD detection comprises a main shell, an information acquisition unit, a display unit and a driving unit. The main shell is provided with an access hole, and a bearing plate is arranged in the main shell. A reagent strip fixing table is arranged on the bearing plate and is arranged to slide along the depth direction of the access hole. A plurality of operation stations are arranged in the stroke of the reagent strip fixing table. The information acquisition unit is arranged on the bearing plate. The display unit is arranged on the main shell and is electrically connected with the information acquisition unit. The driving unit is arranged on the bearing plate and is connected with the reagent strip fixing table. The purpose is to solve the problems of complicated steps and inconvenient operation of the current dry-type fluorescence immunoassay analyzer in use.