39 results about "FLUORESCENT IMMUNOASSAY" patented technology

The utility model relates to the technical field of detection equipment, in particular to a fluorescence immunoassay analyzer. Comprising a machine body, a detection window is arranged on the front side of the machine body, a multi-channel reaction disc is arranged on one side of the detection window, a plurality of incubation grooves are formed in the front side of the multi-channel reaction disc, the multi-channel reaction disc comprises a plurality of independently arranged timing modules, buzzers and indicator lamps, and the buzzers and the indicator lamps are electrically connected with the timing modules. When the reaction time of the test card on a certain channel in the multi-channel reaction disc is over, the buzzer and the indicator light can give an alarm; the device can immediately detect a plurality of temperature-controlled test cards in sequence, and does not need to wait for the completion of the temperature control of the next test card, so that a large amount of time can be saved.

The application belongs to the field of nanometer material in environmental monitoring application, and discloses a fluorescence immunoassay method for detecting ovalbumin concentration based on carbon quantum dots, which comprises the following steps: using L-glutamic acid as a precursor, one-step preparation of carbon quantum dots with amino groups by ultrasonic hydrothermal method; using a reduced graphene oxide method to prepare graphene sheets, and optimizing the graphene by modifying polydiallyldimethylammonium chloride to obtain graphene sheets which can have good fluorescence resonance energy transfer with the carbon quantum dots with amino groups; and coupling the prepared carbon quantum dots with amino groups with a monoclonal antibody. The application directly prepares carbon quantum dots with amino groups by one-step hydrothermal method, and the quantum dots can be directly coupled with a monoclonal antibody. The carbon quantum dots involved in the application avoid the aminoization step, and the linear range can reach 0.1 ng / mL-3000 ng / mL.

The invention discloses a handheld fluorescence immunoassay analyzer with a dustproof function, and aims to solve the problem that dust and stains easily enter an existing handheld fluorescence immunoassay analyzer from a test paper insertion hole, so that the accuracy of a detection result is influenced. The analyzer comprises an analyzer body and a test paper insertion hole formed in one end of the analyzer body, a dustproof assembly is arranged at the end, close to the outer side of the analyzer body, of the test paper insertion hole, the dustproof assembly comprises a dustproof plate, a driving part and a stirring part, and the dustproof plate can elastically ascend and descend in the test paper insertion hole. During use, the stirring piece is rotated to drive the driving piece to enable the dustproof plate to open the test paper insertion hole, fingers do not need to contact the dustproof plate, insertion of test paper cannot be hindered, and the test paper insertion hole is closed after detection is completed, so that dust is effectively prevented from entering the analyzer in the detection process and after detection is completed; the accuracy of a detection result is improved.

The invention relates to a nano antibody for specifically recognizing a tacrolimus-antibody compound and application of the nano antibody, and belongs to the technical field of biological detection. A variable region of the nano antibody has three complementary determining regions CDR1, CDR2 and CDR3, can specifically recognize a tacrolimus-antibody compound, and can be used for detecting drugs by a double-antibody sandwich method; the kit is widely suitable for various immunodetection technologies such as an enzyme-linked immunosorbent assay (ELISA), a chemiluminescence immunoassay (CLIA), an electrochemical luminescence immunoassay (ECLIA), a fluorescence immunoassay (FIA), an immunochromatography test strip method and the like. According to the method, the limitation of a traditional competition method is overcome, high-sensitivity, high-specificity and high-stability detection is realized, the method can be adapted to various detection platforms, and a portable pretreatment process without centrifugation can also be realized.

The invention discloses a fluorescence immunoassay analyzer test fixture and a test method thereof, and belongs to the technical field of medical instrument tests, the fluorescence immunoassay analyzer test fixture comprises a first assembly body and a second assembly body movably connected with the first assembly body; the first assembly body is of a plate-shaped structure, the second assembly body is of a hollow box-shaped structure with a top cover and without a bottom cover, and the first assembly body is matched with the second assembly body; the first assembly body is of a double-layer structure and comprises a bottom plate and a fixing plate arranged on the bottom plate. Wherein the fixing plate is provided with a plurality of fixing grooves and fixing columns which are matched with the fluorescence immunoassay analyzer; clamping grooves matched with the second assembly bodies are further formed in the four corners of the fixing plate. The fluorescence immunoassay analyzer is accurately fixed and positioned through detachable connection of the second assembly body and the first assembly body, and the technical effects of simplifying the test operation process and improving the test stability and safety are achieved.

The invention discloses a near-infrared fluorescence immunoassay analyzer, and relates to the field of biomedical engineering.The near-infrared fluorescence immunoassay analyzer comprises an analyzer body, a dome-type fluorescence collector is installed in an inner cavity of a sealing seat, a narrow-band light filter is installed at the bottom of the dome-type fluorescence collector, and a piezoelectric focusing lens set is installed at the bottom of a light source sliding block; a light source sliding block is arranged in the analyzer body, a laser range finder and an optical fiber beam splitter are respectively arranged on two sides of the light source sliding block, a double-layer reagent card base body is arranged in the center of the top of the analyzer body, a snakelike microfluidic channel is formed in the top of the double-layer reagent card base body, a shape memory alloy is arranged at the bottom of the double-layer reagent card base body, and a thermosensitive actuator array is arranged at the bottom of the shape memory alloy. According to the near-infrared fluorescence immunoassay analyzer, eight-path parallel scanning is combined with dynamic focusing, so that the single-sample full-index detection time can be greatly shortened, the near-infrared fluorescence immunoassay analyzer can be compatible with various complex biological samples, a chemical color developing agent is not needed, and the experiment cost and the environmental pollution risk are reduced.

The invention discloses a fluorescence immunodetection analyzer and a detection analysis method thereof. The analyzer comprises a sheath flow pool which is vertically arranged, red and green laser excitation light sources which are horizontally arranged on the two sides of the sheath flow pool in the X-axis direction, and double independent light paths which are arranged on the two sides of the sheath flow pool in the Y-axis direction. A red laser light path separates fluorescence into three paths of signals of 730nm, 670nm and residual fluorescence through a series-connected dichotomy mirror group; the green laser light path collects diffuse reflection light. The four paths of optical signals are converted into voltage signals by four detectors, temperature compensation units integrated in the detectors correct the signals in real time based on a pre-calibrated dark current-temperature relation curve, and temperature drift is suppressed. And the signal processing system calls a pre-established signal model library to carry out comprehensive calibration and synchronous analysis on multi-channel signals, so that accurate distinguishing and counting of different fluorescent microspheres are realized. According to the invention, the problems of large temperature influence and low detection efficiency in the prior art are solved, and the stability, accuracy and multi-channel consistency of detection are remarkably improved.

The utility model discloses an optical detection structure and portable fluorescent immunoassay appearance. Optical detection structure includes first seat body, light source structure, second seat body, optical component, and the first seat body is formed with the first light guide hole and is linked with the first light guide hole first light hole, and second seat body is connected with first seat body, and is seted up with the second light guide hole opposite first light guide hole, and the light source structure's luminous part is close to first light hole setting, and optical component is located in first light guide hole and second light guide hole. The utility model integrates light source structure and optical component in one, compared with common 45 degree two -way spectroscope adds filter piece scheme, and the optical part required is less, and the structure is more compact, and the volume of whole optical detection structure is smaller, can be better applicable to portable fluorescent immunoassay appearance, and moreover, assembling is more convenient, can better guarantee the accuracy of the light hole and optical component alignment on light source structure.

1. The name of the design product: fluorescent immunoassay analyzer (RealG-FL12 Pro). 2. The use of the design product: a medical testing device for detecting the analyte in human whole blood, serum and plasma samples, etc., using fluorescent immunoassay technology to excite and measure the fluorescent signal, thereby realizing rapid identification and quantitative analysis of pathogens, and providing important information for the diagnosis and treatment of infectious diseases. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The utility model discloses an electronic fluorescence test paper disease detection pen type sensor, which adopts a dichroscope, a purple optical filter and a red optical filter to form an optical path, and reduces the use of a lens group compared with an existing fluorescence immunochromatography instant detector. A control circuit module and a photoelectric detection module are integrated in the electronic fluorescence test paper disease detection pen type sensor, and the sensor has the advantages of being small in size, compact in structure and convenient to carry.

The invention discloses a kit for rapidly detecting sST2. The kit comprises a test strip, the test strip comprises a back plate, and a water absorption pad, an NC membrane, a fluorescent pad and a sample pad which are sequentially laid on the back plate, the fluorescent pad is coated with a second monoclonal antibody which is labeled by fluorescent microspheres and is combined with a C-terminal unique region of sST2; the NC membrane is provided with a detection strip and a quality control strip, the detection strip is coated with a first monoclonal antibody combined with a third immunoglobulin structural domain of sST2, and the quality control strip is coated with a second antibody for resisting a second monoclonal antibody; according to the kit, rapid detection of sST2 can be completed within 6 minutes, cross reaction between isomers (such as soluble ST2 and transmembrane type ST2) is effectively eliminated, the detection accuracy and sensitivity are improved, the kit can directly use a whole blood sample for detection, centrifugation is not needed, and the detection time is saved; and the method is suitable for a full-automatic fluorescence immunoassay analyzer, and can realize automatic, convenient and rapid interpretation.

1. The name of the design product: fluorescent immunoassay analyzer (RealG-Fluo-2000). 2. The use of the design product: for detecting the measured object in human whole blood, serum and plasma samples, etc. by detecting the measured object in human whole blood, serum and plasma samples, etc. by using fluorescent immunoassay technology to excite and measure the fluorescent signal, so as to realize the rapid identification and quantitative analysis of pathogen. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The utility model provides a fluorescence immunoassay detector device which comprises a detector and a fluorescent reagent strip, and the detector is used for detecting the fluorescent reagent strip; a tooth-shaped structure is arranged at the bottom of the fluorescent reagent strip; the detector comprises an objective table, a reagent strip insertion opening, a detection device, a transmission gear and a clockwork spring device, wherein the detection device is provided with at least one detection site on the objective table; the reagent strip insertion opening allows the fluorescent reagent strip to enter or come out, the transmission gear is rotationally arranged on the objective table and is meshed with a tooth-shaped structure at the bottom of the fluorescent reagent strip, and the clockwork spring device drives the transmission gear to rotate; and the transmission gear rotates to drive the fluorescent reagent strip to enter the detection site or be separated from the detection site from the reagent strip insertion opening. According to the fluorescent reagent strip detection device, the damping spring structure is adopted for transmission for scanning detection of the fluorescent reagent strip, the electric quantity required by the whole detection device is small, the cost is greatly reduced, and the fluorescent reagent strip detection device is more suitable for household detection scenes.

The application discloses a reagent card bearing mechanism of a fluorescent immunoassay device, which comprises a support for providing basic support, a bearing plate rotationally connected with the support, the bearing plate comprising a main body part and a plurality of clamping parts arranged along the center of the main body part in a circumferential array, air passages being arranged between the clamping parts and the main body part, one end of each air passage being connected in communication with the center of the main body part, and adjacent two clamping parts and the main body part forming a card placement area of a reagent card; and a temperature control assembly comprising a heating plate, a temperature sensor, a fan and a control unit, the control unit controlling the working conditions of the heating plate and the fan according to the feedback of the temperature sensor, so that the card placement area is in a preset temperature interval. The reagent card bearing mechanism of the fluorescent immunoassay device has the beneficial effects of rapid temperature adjustment and excellent constant temperature effect.

The invention relates to the technical field of biological medicine detection, in particular to a method for detecting apramycin by using a quantum dot fluorescence immunoassay method. A sample and an apramycin monoclonal antibody solution are added into a coated elisa plate for incubation, then a fluorescent probe solution is subjected to incubation reaction, the fluorescence intensity is measured, and finally the apramycin content in the sample is calculated. CdSe / ZnS quantum dots and rabbit anti-mouse IgG are combined to serve as a novel fluorescent probe, a fluorescence immunoassay method is established and used for quantitative detection of apramycin in animal feed, and compared with a traditional indirect competitive enzyme-linked immunosorbent assay method, the fluorescence immunoassay method is more sensitive.

A sample solution preparation method, a measurement unit, a measurement kit, and a sample solution preparation device for measuring a target compound contained in a food by using a fluorescent immunoassay method. The features of the present disclosure include a dialysis step for bringing a target compound-containing solution including a target compound into contact with a dialysate through a dialysis membrane to transfer the target compound to the dialysate, wherein the molecular weight cut-off of the dialysis membrane is in the range of 2 x 10 2 to 2 x 10 5 , and the volume of the dialysate is relatively smaller than the volume of the target compound-containing solution.

The application provides a glycated hemoglobin dissociation reagent and application thereof, and belongs to the technical field of biological detection. The dissociation reagent comprises 10-50 mM Tris buffer, 0.2-0.5 M ammonium chloride, 0.5%-1.0% (v / v) triton X-100, 0.2 wt%-1.0 wt% BSA and 0.02-0.2 M sodium chloride. When the dissociation reagent is used to dissociate a whole blood sample, the hemoglobin can be dissociated by mixing the dissociation reagent with the whole blood sample for 20-60 s, and a clear and transparent solution is obtained. The dissociation reagent can be used to prepare a kit for dissociating or / and detecting glycated hemoglobin. The glycated hemoglobin obtained by using the dissociation reagent can be directly used for fluorescence immunoassay, the interference of the color of hemoglobin itself on immunochromatographic detection is avoided, and the sensitivity is high and the repeatability is good.

The utility model belongs to the technical field of quantum dot fluorescence immunoassay analyzers, and particularly relates to a sample test tube conveying rack of a quantum dot fluorescence immunoassay analyser, which comprises a mounting plate mounted on an analyzer body, a first magnetic plate arranged on the outer side of the mounting plate, a first sliding groove formed in the mounting plate, and a conveying assembly arranged in the first sliding groove, a threaded block is arranged on the conveying assembly, a second sliding groove is formed in the threaded block, an elastic assembly is arranged in the second sliding groove, a connecting block is installed on the elastic assembly, a test tube rack is installed on the connecting block, and a plurality of through holes allowing reagent tubes to enter are formed in the test tube rack; a second magnetic plate and a third magnetic plate which are connected with the connecting block are arranged on the outer side of each through hole, and the positions of the second magnetic plates and the positions of the third magnetic plates correspond to the positions of the first magnetic plates. According to the device, the reagent tube can be vibrated during conveying, a solution in the reagent tube is effectively prevented from precipitating, meanwhile, the cost is low, and subsequent maintenance is facilitated.

The application discloses a multifunctional portable single-channel detection device, which comprises a shell, a detection port and an incubation port, a fluorescent immunoassay component, a colloidal gold detection component, a constant-temperature incubation box, a mobile positioning mechanism, a microprocessor and a touch display screen arranged in the shell; a reagent card can be placed into the constant-temperature incubation box through the incubation port; the reagent card can be transferred to the fluorescent immunoassay component or the colloidal gold detection component by the mobile positioning mechanism for detection through the detection port; the microprocessor is electrically connected with other components, and the touch display screen realizes man-machine interaction; the application has the characteristics of multifunction, miniaturization and easy portability, and has a simple structure and is easy to mass produce.

This invention discloses a monoclonal antibody against rabies virus N protein, a detection reagent, and their applications. The monoclonal antibody against rabies virus N protein includes a heavy chain variable region and a light chain variable region; the amino acid sequence of the heavy chain variable region is shown in SEQ ID No. 1; the amino acid sequence of the light chain variable region is shown in SEQ ID No. 2. This invention provides a monoclonal antibody with high affinity and detection sensitivity against rabies virus N protein, laying the foundation for the research and development and promotion of colloidal gold test strips and fluorescent immunoassay strips.

The utility model discloses a fluorescence immunoassay reagent card detection device, which comprises a protective shell, a reagent card transmission inlet, a reagent card transmission outlet, a fluorescence immunoassay reagent card detection module, a fluorescence immunoassay reagent card detection module, a fluorescence immunoassay reagent card detection module and a fluorescence immunoassay reagent card detection module, the plurality of conveying channels are fixedly arranged in the protective shell, are used for conveying the reagent cards to be detected, are arranged in parallel, are positioned on the same horizontal plane, and are respectively communicated with the reagent card conveying inlet and the reagent card conveying outlet; the optical assembly is fixedly arranged in the protective shell, is positioned right above the plurality of conveying channels, and faces the detection areas of the conveying channels so as to perform fluorescence signal detection on the plurality of reagent cards at the same time; and the driving assemblies are arranged in the protective shell and are arranged in a one-to-one correspondence manner along the lower parts of the plurality of conveying channels so as to drive the reagent cards to move along the conveying channels.

The invention relates to the technical field of fluorescence immunoassay, in particular to a fluorescence immunoassay kit for detecting horse adrenocorticotropin and a preparation method of the fluorescence immunoassay kit. The kit comprises a sample diluent with specific components and a test strip, the test strip is provided with a pretreatment pad marker combination pad, and a fluorescent microsphere labeled antibody mixed solution sprayed on the test strip can be efficiently combined with a target substance. The pretreatment pad, the marker combination pad and a sample diluent have a synergistic effect, so that sample interference is effectively eliminated, the chromatography rate is regulated and controlled, the reaction microenvironment is stabilized, the activity loss of a target object is reduced, non-specific combination is realized, and an optimized preparation process is matched, so that the detection accuracy and reliability are remarkably improved, and the detection performance is excellent in a wide concentration range. Meanwhile, the standard preparation process ensures the product quality uniformity, is suitable for batch production, fills the blank in the related detection field, and has good practical application value and wide prospects.

This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a gold-plated solid surface bound with a fluorescent label, a light source, a light correcting lens, and an optical detector to detect the fluorescent emission light. The solid is made of plastic doped with glass fibers and the solid surface is coated with gold, and the hardness of the solid is at least 75 based on Rockwell M hardness scale. The system provides high sensitivity and low variability for fluorescent immunoassays.

The application belongs to the technical field of IVD detection, and particularly relates to a dry-type fluorescence immunoassay analyzer for IVD detection. The dry-type fluorescence immunoassay analyzer for IVD detection comprises a main shell, an information acquisition unit, a display unit and a driving unit. The main shell is provided with an access hole, and a bearing plate is arranged in the main shell. A reagent strip fixing table is arranged on the bearing plate and is arranged to slide along the depth direction of the access hole. A plurality of operation stations are arranged in the stroke of the reagent strip fixing table. The information acquisition unit is arranged on the bearing plate. The display unit is arranged on the main shell and is electrically connected with the information acquisition unit. The driving unit is arranged on the bearing plate and is connected with the reagent strip fixing table. The purpose is to solve the problems of complicated steps and inconvenient operation of the current dry-type fluorescence immunoassay analyzer in use.

The application discloses a modular and splicable multifunctional detection analyzer, and belongs to the technical field of micro-fluid detection and fluorescent immunoassay, which comprises a first detection component, a second mounting shell, a first mounting shell, a display component and a second detection component, a plurality of second mounting shells are mounted on the top of the first mounting shell, the display component is arranged on the side of the first mounting shell, the first detection component is mounted in the second mounting shell, the second detection component is mounted in the first mounting shell, and a part of the second detection components are transmissionally connected in the first mounting shell. The multifunctional detection analyzer solves the problems of large occupied area and low detection efficiency caused by the fact that a plurality of devices cannot be stacked and data transmission is difficult in the prior art. The multifunctional detection analyzer improves detection efficiency, improves universality and reduces cost.

The utility model discloses an auxiliary structure for fluorescent immune layer detection reagent calibration, which relates to the technical field of reagent calibration and comprises a guide hopper, a mixing cylinder is arranged in the middle of the bottom end of the guide hopper, a sealing tube is detachably mounted at the bottom of the mixing cylinder, a thin film is arranged at the bottom of the sealing tube, and the thin film is arranged in the guide hopper. Stand columns are arranged at the four corners of the bottom end of the guide hopper, the bottom ends of the four stand columns are jointly connected with an upper sealing plate, a crevasse converter is placed at the top end of the upper sealing plate, the top of the crevasse converter is matched with the bottom of a sealing pipe, a liquid outlet is formed in one side of the crevasse converter, and a liquid outlet is formed in the other side of the crevasse converter. A crevasse cone is arranged in the middle of the interior of the crevasse converter. According to the utility model, a series of structures are arranged, so that a reagent can be dropped on the test paper without a dropper or other tools during operation, the situation that liquid cannot accurately fall on the preset position of the test paper due to hand shaking or other factors of an operator is avoided, and the calibration efficiency is improved.

The invention belongs to the technical field of immunodetection analysis, and discloses a kit for quantitative detection of a thrombin-antithrombin III compound and a preparation method of the kit. The kit comprises a magnetic bead working solution, an acridinium ester marker working solution, a biotin marker working solution, a TAT calibrator and a TAT quality control product, and quantitative detection of TAT is realized by combining a double-antibody sandwich method with a chemiluminescence technology. The method overcomes the defects that an existing fluorescence immunoassay method is low in repeatability, and an immunoturbidimetry method is high in requirement for specimens and limited in low-concentration detection capacity, and has the advantages of being high in specificity, sensitivity and accuracy and easy, convenient and rapid to operate. The method can be used for clinical auxiliary diagnosis of thrombogenic diseases such as disseminated intravascular coagulation (DIC) and venous thromboembolism (VTE), and provides a basis for blood coagulation state evaluation and clinical decision making.

The invention provides a fluorescence immunoassay method and system based on big data. Belongs to the technical field of biosensing and big data analysis. The method comprises the following steps: comprehensively collecting physicochemical characteristics of different patient samples, key indexes of each batch of reagents and laboratory environment condition data, constructing a sample, reagent and environment multi-dimensional data association model, dynamically adjusting parameters of sample adding, incubation and washing links based on the constructed sample, reagent and environment multi-dimensional data association model, and calculating the sample, reagent and environment multi-dimensional data association model. Synchronously collecting an original fluorescence signal and operation timestamp data, removing background noise, and generating preliminary noise reduction fluorescence signal data; by comprehensively collecting sample physicochemical characteristics, reagent batch characteristics and environmental condition data, constructing a correlation model and dynamically adjusting analysis link parameters, interference of matrix effect and reagent batch drift on fluorescence immunoassay is eliminated, detection result deviation caused by sample difference and reagent batch change is avoided, and the detection accuracy is improved. And the accuracy and the reliability of a detection result are greatly improved.

The utility model discloses preparation equipment of a double-C-line fluorescence immune layer detection reagent, which relates to the technical field of preparation of the double-C-line fluorescence immune layer detection reagent, and comprises a soaking box, a plurality of partition plates are arranged in the soaking box, the partition plates divide an inner cavity of the soaking box into a plurality of soaking compartments, and the soaking compartments are arranged in the soaking box. Two electric push rods are mounted at the bottom end of the interior of the soaking compartment, a lifting frame is slidably connected to the interior of the soaking compartment, a mounting frame is mounted in the lifting frame, a nitrocellulose membrane is laid in the mounting frame, and sliding glass is detachably mounted on the surface of the mounting frame. According to the utility model, a series of structures are arranged, and different starting time is set for the electric push rods in different soaking compartments, so that the lifting frame can be pushed to the outside of the soaking box at different installation time in sequence in the same environment; the requirements of a user on the nitrocellulose membrane with different soaking time in the same environment can be met.

The application discloses a kind of gold cluster-manganese dioxide nanosheet-based pesticide fluorescence immunoassay method.Currently, it is still challenging to develop a high-sensitivity, strong anti-interference ability analysis method to detect pesticides in complex food matrix.In the present application, coated antigen competes with pesticide to recognize enzyme-labeled antibody.After competitive immune reaction, ALP catalyzes substrate to produce ascorbic acid, which triggers the decomposition of AuNCs-MnO2NFs and regulates fluorescence reaction.Methidathion is selected as the analyte to be detected by the method.The results show that the fluorescence immunoassay method has high detection sensitivity (IC 10 = 5.78 pg mL ‑1 ), which is about 56 times higher than the traditional enzyme-linked immunoassay.The present application is successfully used for precise monitoring of pesticide residues and degradation dynamics in pakchoi, and has good anti-interference ability and reproducibility, paving the way for pesticide residue determination in actual samples.