57 results about "FLUORESCENT IMMUNOASSAY" patented technology

Disclosed are an automated card withdrawal mechanism and a single-channel fluorescent immunoassay instrument. The automated card withdrawal mechanism comprises a base plate (10), a slide rail (20), a sliding mechanism (30), a position blocking mechanism (40) and a card pushing mechanism (50), wherein the base plate (10) is provided with a long strip-shaped hollowed part (11), a card withdrawal slideway (12) is fixed under the base plate (10), a first end of the card withdrawal slideway (12) is provided with a slideway entrance enabling a test card (70) to go in and out; the slide rail (20) is mounted on the base plate (10) and is close to the hollowed part (11); the sliding mechanism (30) is movably mounted on the slide rail (20) and can move in the direction of the slide rail (20), and the test card (70) can be placed on the sliding mechanism (30); the position blocking mechanism (40) is fixed on the base plate (10) and at a position near a second end of the card withdrawal slideway (12); and the card pushing mechanism (50) is mounted on the sliding mechanism (30) and partially extends into the card withdrawal slideway (12), and can push the test card (70) out of the slideway entrance of the card withdrawal slideway (12), realising automated card withdrawal. The mechanism can realise active card pushing with reduced card withdrawal failure and high stability, and testing efficiency can be improved.

The invention discloses a handheld fluoroimmunoassay device. The device comprises a shell and an upper cover, the shell and the upper cover enclose an accommodating chamber, a fluorescence data acquisition module, an photoelectric scanning module and a master control module are arranged in the accommodating chamber, and the surface of the upper cover is provided with a touch screen; the fluorescence data acquisition module is arranged above the photoelectric scanning module, and the master control module is respectively connected with the fluorescence data acquisition module, the photoelectricscanning module and the touch screen; the device also comprises a pedestal bottom with a reagent card slot integrated to the photoelectric scanning module; and the position of the fluorescence data acquisition module in the accommodating chamber is moving. The fluorescence data acquisition module includes a plurality of excitation light sources which are light sources having different wavebands respectively, so multi-purpose use of one machine is realized, the detection of multiple fluorescent band samples can be achieved, and the application range of the device is enlarged; and the device adopting the moving fluorescence data acquisition module and fixed reagent card structure has the advantages of compact integral structure layout, small size, and easiness in realization of handheld operation and carrying.

A fluorescence immunoassay device based on integration of a photonic crystal and magnetic beads and a method thereof are provided. Magnetic beads with high surface-to-volume ratio are used as carriers of fluorescent molecules to obtain higher fluorescence density. The electric field on the surface of the photonic crystal is enhanced through excitation of photonic crystal resonance. The intensity of the fluorescence signal excited by the enhanced electric field is increased. Moreover, through interaction with the photonic crystal, some fluorescent signals that originally cannot be received by the fluorescent sensor are coupled to the photonic crystal resonant modes and reradiate toward the fluorescent sensor, thereby increasing collection efficiency. The fluorescence signals generated by fluorescent molecules on the magnetic beads are significantly intensified, which could lower the detection limit. Furthermore, the magnetic beads aggregation method can achieve the detection capability that cannot be achieved by the current fluorescent immunoassay.

The invention relates to a method for judging the hook effect of homogeneous time-resolved fluorescence immunoassay. The fluorescence intensity ratio of two wavelengths is used as the signal value; (2) by measuring the signal values ​​at least two moments in the initial stage of the reaction, by linear fitting and four-parameter fitting, the absolute value of the signal intensity and the rate value of the signal change are respectively established. and the standard curve of the sample concentration; (3) detect the signal value of the unknown sample, and obtain the concentration of the unknown sample according to the fluorescence intensity of the unknown sample through the standard curve, thereby avoiding the hook effect. Using the ratio of the absolute value of the signal intensity and the value of the rate of change of the signal to the background signal, the relative signal intensity and the relative value of the rate of change of the signal are calculated to achieve consistent results on different instruments. The concentration interpretation of more samples can be completed within a given time, so that the system has higher detection throughput.

The invention discloses a PCT (Procalcitonin) and CRP (C-Reactive Protein) double-label time resolution fluorescence immunoassay method for simultaneously detecting bacterial meningitis and viral meningitis. A recombinant polypeptide antigen is included and the amino acid sequence is shown as SEQ ID NO: 1. The recombinant polypeptide antigen is used as an immunogen, and a PCT antibody and a CRP antibody are obtained by screening respectively through immune treatment; double-label time resolution fluorescence immunoassay is carried out. The method disclosed by the invention is high in sensitivity, is rapid, is simple and convenient and is easy to popularize clinically. The whole detection time of a kit utilizing the method is less than 2 hours, and suspected patients with the meningitis can be rapidly screened at an early stage. Reliable evidences are provided for specific treatment of children with the meningitis; pains of the children with the meningitis are alleviated and the diagnosis time of diseases is shortened; valuable time for the specific treatment is won, so that medical cost and a lot of manpower and material resource expenditures are saved, and worries and panics of parents are also alleviated.