Gene chip and method for detecting classical swine fever virus (CSFV), porcine circovirus virus type 2 (PCV-2) and porcine reproductive and respiratory syndrome virus (PRRSV)

Abstract

The invention provides a gene chip detection method for a classical swine fever virus (CSFV), a porcine circovirus virus type 2 (PCV-2), a porcine reproductive and respiratory syndrome virus Europe type (PRRSVE) and a porcine reproductive and respiratory syndrome virus America type (PRRSVA). A gene chip is shown as a probe sequence in the table of the specification. The invention discloses a method for simultaneously detecting three diseases, namely classical swine fever (CSF), a porcine circovirus type 2 (PCV-2) and a porcine reproductive and respiratory syndrome (PRRS) by using the gene chip. By the method, the problems that the conventional detection technology is time-consuming and labor-consuming and has low specificity, or only can be used for detecting a single disease are solved. All probes provided by the invention are synthesized into a connecting amino group at a 5' end, a poly T connecting arm with the length of 15 bp is provided, and a result shows that fixing efficiency is relatively high. By a polymerase chain reaction (PCR) amplification technology with mark primers, various kinds of fluorescent mark deoxyribonucleic acid (DNA) complementary to corresponding probescan be amplified at a time, and the high specificity and sensitivity of nucleic acid hybridization in a solid-liquid phase can be ensured. In addition, by a mark method, detection time is greatly shortened, the cost of chip detection is lowered, and the detection method is more suitable to be popularized in clinical application.

Description

technical field [0001] The invention relates to the fields of biological gene technology and computer data analysis, in particular to a method for detecting classical swine fever virus (Classical Swine Fever Virus, CSFV), porcine circovirus type 2 (Porcine Circovirus type 2, PCV-2) and pig reproductive The invention relates to a gene chip of Porcine Reproductive and Respiratory Syndrome Virus Europe type and America type (PRRSE, PRRSVA) and a method for preparing the chip, and a method for detecting or diagnosing the virus by using the gene chip. Background technique [0002] With the increase of large-scale pig farms, the types of swine diseases are also increasing. The old diseases have not been eliminated, and new diseases continue to appear. With the variation of virus strains, the diseases of pigs are increasingly complicated and serious. Classical Swine Fever (CSF), porcine circovirus type 2 (PCV-2) and porcine reproductive and respiratory syndrome (Porcine Reproductiv...

AI Technical Summary

Problems solved by technology

[0004] In view of the time-consuming and labor-intensive detection and high cost in the prior art, and the deficiency that only a single disease can be detected, the purpose of the present invention is to provide a kind of swine fever, Porcine circovirus disease and porcine reproductive and respiratory syndrome virus gene chip, and the preparation method of the chip;

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