23 results about "Classical swine fever" patented technology

The application discloses an African swine fever virus attenuated strain with I215L protein deletion, a live vaccine preparation method and application thereof, and belongs to the technical field of biotechnology, and particularly relates to an African swine fever virus attenuated strain with I215L protein deletion, a live vaccine preparation method and application thereof. The recombinant virus prepared by the application is an African swine fever virus I215L protein function deletion strain, the I215L partial gene is deleted, and the immunization of the weak strain with the gene deletion can achieve 100% immune protection effect on homologous II type ASFV and heterologous I / II type ASFV, and meanwhile, there is no residue in the body, so that the application has a wide application prospect.

This invention relates to the field of biotechnology, and aims to provide a monoclonal antibody 7A12 for identifying genotype 2 classical swine fever, along with its preparation and application methods. This monoclonal antibody contains an Ig domain V of the antibody heavy chain. H CDR1, V H CDR2 and V H CDR3, and the Ig domain V of the antibody light chain L CDR1, V L CDR2 and V L CDR3; wherein, the V H CDR1, V H CDR2 and V H The amino acid sequences of CDR3 are shown in SEQ ID NO:1-3, respectively; the V L CDR1, V L CDR2 and V L The amino acid sequences of CDR3 are shown in SEQ ID NO:4-6. The antibody provided by this invention has advantages such as high titer, strong specificity, and high affinity; it also has high sensitivity and specificity, and shows no cross-reactivity with type 1 classical swine fever vaccine strains. Serologically, it can differentiate and diagnose infection and vaccine immunization with genotype 2 classical swine fever wild-type strains. Furthermore, it can be used to develop a blocking ELISA kit for identifying genotype 2 classical swine fever, effectively diagnosing and purifying genotype 2 classical swine fever virus.

This utility model relates to the field of African swine fever detection technology and discloses an African swine fever detection device, including a detection platform and a detection slot opened at the top of the detection platform. The mixing component includes a mixing platform and a mixing hole slot opened at the top of the mixing platform. A dilution bottle is installed inside the mixing hole slot, and an internal threaded cap is installed at the top of the dilution bottle. A mixing cap is installed at the top of the mixing platform and at the top of the internal threaded cap. The capacity of the mixing component can be adjusted according to different detection items. By rotating the lower tube, the inner tube moves down from inside the outer threaded tube, and the capacity inside the upper tube and the lower tube increases. The enlarged dilution bottle is inserted into the mixing hole slot. The outer threaded tube is rotated downward, and the space for placing the dilution bottle increases. After increasing the space capacity for mixing blood samples and diluents, the space for placing dilution bottles for mixing is also deepened, which facilitates the mixing of different blood samples and diluents.

The application belongs to the technical field of animal vaccines, and particularly relates to an O-A-Dex-Ap for African swine fever immunization. Based on the good biocompatibility of Dextran, the application uses the Dextran as a nanoparticle to load a coupled CTL epitope peptide. Preliminary experimental results show that, compared with the epitope peptide alone, the O-A-Dex-Ap after loading and coupling can more effectively promote antigen presentation and activate more persistent CTL immune response, and has good application potential. Based on the results, a good technical foundation can be laid for subsequent preparation of African swine fever and other disease vaccines.

The present application belongs to the field of molecular biology detection, and particularly relates to a composition, method and use for simultaneous detection and differentiation of swine fever virus, psittacosis chlamydia, porcine reproductive and respiratory syndrome virus and porcine circovirus type II. The composition for simultaneous detection provided by the present application can realize rapid detection of four pathogen target genes in one tube of reaction solution by using multiplex fluorescence RPA detection technology, and has the advantages of high throughput, short time and low cost. On one hand, the present application can accurately detect pathogens and analyze mixed infection of pathogens, so as to achieve the effect of precise medication, reduce the use of antibiotics, save costs, and reduce the cost and increase the benefit of enterprises, and on the other hand, compared with fluorescence PCR, the detection time is greatly shortened, and the purpose of rapid detection is achieved.

This invention relates to the field of African swine fever (ASF) eradication technology. The invention provides a method for managing ASF-affected populations in a zoned manner while simultaneously managing production, comprising the following steps: (1) screening pigs with abnormal body temperature using a thermal infrared imager; (2) taking tonsil swab samples from pigs with abnormal body temperature and performing ASF qPCR testing; (3) designating positive pigs as "Pig Zero" and immediately initiating zoned management; (4) marking materials, tools, and other items that came into contact with "Pig Zero"; (5) screening areas that had air contact with "Pig Zero" within 7 days; and (6) dividing the pigsty into high-risk, medium-risk, and low-risk zones for separate treatment. The technical management method of this invention can effectively control the impact of ASF on production, is applicable to large-scale pig farms, and achieves precise control, dynamic management, and a closed-loop biosafety system, meeting the core demands of large-scale pig farms for "stable production, cost reduction, and rapid recovery."

The application provides a primer probe combination for identifying the genotype of African swine fever virus, comprising a primer probe combination a for identifying a B646L gene, a primer probe combination b for identifying an MGF-110-5L gene and a primer probe combination c for identifying an I177L gene. The method for identifying the genotype of African swine fever virus by using the primer probe combination is as follows: extracting the genomic DNA of a sample to be detected, using the extracted genomic DNA of the sample to be detected as a template, performing a triple fluorescent quantitative PCR reaction by using the primer probe combination, and judging the genotype of African swine fever virus according to the fluorescent signal. The application also provides the application of the primer probe combination to the preparation of a kit for identifying the genotype of African swine fever virus. The concentration and reaction conditions of the primer probe combination are optimized, so that the designed primer probe combination has strong specificity, sensitivity and repeatability, not only solves the identification problem of African swine fever virus, but also simultaneously realizes the synchronous identification of three kinds of epidemic strain genotypes.

The present invention relates to a biological detection chip for African swine fever virus and its application thereof. The biological detection chip comprises a plurality of transistors connected in parallel, each of which individually includes a substrate layer, a floating gate, a metal connecting channel, an extending gate, and a biological detection layer. The biological detection chip is functionalized through surface modification process to integrate biological probes, enabling the detection of African swine fever virus in test samples.

The application provides a method for diagnosing and differentiating classical swine fever based on a classical swine fever E2 subunit vaccine, which comprises the following steps: immunizing pigs in a pig farm with the classical swine fever E2 subunit vaccine, detecting E0 antibodies and E2 antibodies after one year of immunization, and eliminating the pigs with E0 antibodies until both the E0 antibodies and the CSFV nucleic acid detection are negative, which indicates that the classical swine fever is purified. The classical swine fever E2 subunit vaccine comprises a nucleic acid fragment encoding an antigen protein with the amino acid sequence of SEQ ID NO: 5 inserted into a recombinant expression vector. The classical swine fever E2 subunit vaccine provided by the application has good immunoprotective efficacy on pigs, and a classical swine fever epidemic disease purification method is established by using the subunit vaccine. The application effect of the method in a pig breeding company for two years shows that the method can effectively purify the classical swine fever, which indicates that the purification method provided by the application is feasible and can be popularized and applied.

The application relates to the technical field of prevention and treatment of porcine cecum membrane virus infection, and in particular to a lactobacillus rhamnosus engineering strain expressing small intestine trefoil factor TFF3 and application of the lactobacillus rhamnosus engineering strain in preparation of a porcine cecum membrane virus infection prevention and treatment composition. The lactobacillus rhamnosus engineering strain expressing small intestine trefoil factor TFF3 has the effects of enhancing the natural immune function of pigs, improving the antioxidant defense function and the antiviral activity. The engineering strain can effectively prevent and treat cecum membrane virus infections such as porcine epidemic diarrhea virus, porcine reproductive and respiratory syndrome virus and African swine fever virus, and significantly reduces the incidence of diarrhea of piglets and the mortality rate of infected pigs. Meanwhile, the strain has good biological safety, and can be used as a feed additive for long-term use to realize the prevention of diseases in addition to being used as an effective component of a medicine.

The application belongs to the technical field of biological medicine, and particularly relates to an African swine fever virus p30 protein antigen epitope peptide, a monoclonal antibody and application. The African swine fever virus p30 protein antigen epitope peptide is located at 140-155 aa of the p30 protein, and the amino acid sequence is shown as SEQ ID NO:1. The nucleotide sequence of the heavy chain variable region of the monoclonal antibody is shown as SEQ ID NO:3; and the nucleotide sequence of the light chain variable region is shown as SEQ ID NO:4. The monoclonal antibody can specifically recognize the antigen epitope peptide. The specific recognition of the antigen epitope peptide provides a basis for establishing an efficient method for detecting the African swine fever virus, and also lays a foundation for studying the structure and function of the p30 protein.

The invention pertains to a live attenuated African swine fever virus Georgia 2007 (ASFV-G) Δ9GL / ΔUK strain for use in a vaccine for protecting a pregnant swine against an infection with African swine fever virus (ASFV) by administering the vaccine comprising the live attenuated ASFV-G-Δ9GL / ΔUK strain to the pregnant swine.

The application discloses an African swine fever full-chain multi-link risk early warning method based on TAER-TCN, and belongs to the field of animal disease prevention and control and intelligent food safety monitoring. The method integrates full-link data of pork circulation, constructs an integrated risk control system, takes a time attention-error recursive time convolution network as a core, extracts long time sequence features, strengthens key risk information, suppresses prediction errors, and improves complex scene early warning accuracy. Relying on an asymmetric causal kernel function, the method analyzes risk cross-link transmission paths, completes risk traceability positioning, realizes four-level graded early warning according to a risk index, and matches corresponding disposal schemes. The application solves problems of traditional technology link fragmentation, early warning lag, data island, traceability difficulty, false and missed report rate and the like, has advantages of full-chain coverage, early prediction, high precision, easy deployment and the like, is suitable for pig slaughtering, cold chain transportation, processing and storage and market sales scenes, and provides intelligent technical support for pig industry safety and food disease prevention and control.

The present disclosure belongs to the field of biotechnology, and specifically relates to a seven-component antigen African swine fever subunit vaccine. The present disclosure first provides an African swine fever virus antigen protein combination composed of the African swine fever virus P34 protein, P30 protein, P54 protein, A104R protein, C129R protein, X protein, and Y protein. This African swine fever virus antigen protein combination can induce a strong immune response in the host. Furthermore, the present disclosure provides a seven-component antigen African swine fever subunit vaccine including the aforementioned African swine fever virus antigen protein combination. The seven-component antigen African swine fever subunit vaccine exhibits good immunoprotection rates against challenge with the parental virulent African swine fever virus strain, poses no biosafety risks, overcomes the difficulty that existing African swine fever subunit vaccines domestically and internationally cannot provide effective immunoprotection for pigs, and demonstrates the feasibility of the research approach for African swine fever subunit vaccines.

The present application relates to a kind of African swine fever monitoring purification method and its application, belong to animal epidemic prevention and control technical field.The present application provides a kind of African swine fever monitoring purification method, the monitoring purification method is to the group of African swine fever nucleic acid positive is managed, including the following steps, (1) the first 3 days screening body temperature high abnormal pig and in pig group is removed;(2) the 4th-20th, the breeding environment is purified, and African swine fever qPCR detection is carried out every day, and positive pig individual is removed;(3) the 21th-27th, qPCR detection is carried out to pig group and environment sample, and the detection result is all negative, indicating that purification is completed.The present application can accurately measure African swine fever by combining body temperature and qPCR detection, and environmental disinfection avoids infection again, and the qPCR detection result of pig individual and environment is used as index to determine purification degree, can quickly and effectively purify African swine fever nucleic acid positive pig and breeding environment in short time.

This invention belongs to the field of biomedical technology, specifically relating to a kaempferol-Fe(III) complex for combating African swine fever virus (ASFV), its preparation method, and its application. Kaempferol and Fe(III) are mixed under alkaline conditions at a weight ratio of 50-100:27. The deprotonated and activated phenolic hydroxyl groups in the kaempferol coordinate with Fe(III), driving self-assembly to form a nano-complex, thus obtaining the kaempferol-Fe(III) complex. The kaempferol-Fe(III) complex of this invention effectively overcomes the technical bottlenecks of poor biocompatibility, low water solubility, and significant cytotoxic side effects of kaempferol in practical applications, significantly improving its usability and safety as an antiviral drug. This invention provides a theoretical basis for the biological application of natural plant products and metal complexes, and also provides a basis for the prevention and treatment of ASFV and the development of novel antiviral agents.

The application provides an African swine fever virus detection method and kit based on the properties of four-strand DNA oxidoreductase. The method uses isothermal amplification technology to amplify the genomic fragment containing the four-strand DNA sequence in the African swine fever virus genome, and realizes rapid and visual detection of the African swine fever virus by adding hematin and oxidoreductase substrate for color development.

The present disclosure belongs to the technical field of biological feeds, and in particular relates to use of an Areca catechu product as a sole active ingredient in manufacturing a biological feed and a dinking preparation for preventing and controlling African swine fever. In the present disclosure, the Areca catechu product as the sole active ingredient is mixed with an African swine fever virus (ASFV) suspension, and then inoculated in cells. Evaluation by in vitro cell experiments demonstrates that the Areca catechu product as the sole active ingredient has a good killing effect on ASFV, where 100% of ASFV with a virus titer of 5×103 HAD50 / mL and can be killed within 30 minutes, and 100% of ASFV with a virus titer of 5×104 HAD50 / mL can be killed within 2 hours.

This invention relates to the field of biotechnology, and particularly to primers and methods for whole-genome sequencing of African swine fever virus (ASFV) based on next-generation sequencing platforms and targeted enrichment. This invention discloses a primer set and method for whole-genome sequencing of ASFV based on next-generation sequencing platforms and targeted enrichment, comprising 32 pairs of specific targeted amplification primers. These primers exhibit high specificity and sensitivity, fully leveraging the advantages of next-generation sequencing—high throughput, low cost, high accuracy, and mature technology—to accurately and completely obtain the entire ASFV genome sequence. This invention can obtain complete genetic information of ASFV, identify and diagnose infections of different ASFV genotypes, and, by analyzing the genetic differences among different strains, determine the geographical origin and transmission dynamics of circulating strains, tracing the evolution of recombinant strains. It provides resources for basic research on virus-host interactions and drug resistance, and offers a theoretical basis for developing long-term prevention and control strategies. It also promotes innovation in diagnostic methods.

The application relates to a quadruple fluorescent quantitative PCR detection kit for detecting African swine fever virus (ASFV) type I, type II wild strains and / or ASFV-G-DI177L strains, which comprises a set of specific primers and fluorescent probes for detecting the African swine fever virus type I, type II wild strains and / or ASFV-G-DI177L strains, the nucleotide sequence of the specific primers is shown in SEQ ID NO. 1, 2, 4, 5, 7, 8, 10 and 11; and the nucleotide sequence of the specific fluorescent probes is shown in SEQ ID NO. 3, 6, 9 and 12. The application designs a quadruple fluorescent quantitative PCR detection method, four genes can be detected at the same time, and the four pairs of primers and probes have relatively consistent amplification efficiency. The application establishes a method for identifying the African swine fever virus gene type I wild strain, the gene type II wild strain and the ASFV-G-DI177L strain based on the characteristics of domestic African swine fever epidemic strains.

The application discloses a CaO@SiO2 / multi-walled carbon nanotube composite material, a preparation method and application, and the preparation method comprises the following steps: step 1: CaO nanoparticles are dispersed in a solvent, a silicon source is added, and the CaO@SiO2 nanocomposite material is obtained through sufficient stirring reaction; and step 2: the CaO@SiO2 nanocomposite material and the multi-walled carbon nanotube are dispersed in a solvent, and the colloidal solution is obtained through ultrasonic mixing, and the colloidal solution is the required CaO@SiO2 / multi-walled carbon nanotube composite material; the electrochemical sensor prepared from the composite material obtained by the application can specifically detect hog cholera virus, the binding capacity of the composite material and a virus specific recognition element is improved, the interference of impurities such as impurities in a sample matrix is reduced, and the detection performance is excellent.

This invention discloses a primer and probe combination for detecting African swine fever virus (ASFV), a PCR detection product, and a method for detecting ASFV, relating to the field of animal disease prevention and control technology. It includes a first primer and probe set, a second primer and probe set, and a third primer and probe set targeting the MGF505 gene. The probes are shown in SEQ ID NO: 1. The first primer and probe set includes upstream and downstream primers as shown in SEQ ID NO: 2-3; the second primer and probe set includes upstream and downstream primers as shown in SEQ ID NO: 4-5; and the third primer and probe set includes an upstream primer as shown in SEQ ID NO: 2 and a downstream primer as shown in SEQ ID NO: 6. The primer and probe combination provided by this invention can detect multiple targets of the ASFV MGF505 gene with high sensitivity and high specificity.

The application provides application of thalidomide in preparation of antiviral drugs and belongs to the technical field of antiviral drugs. The application proves that thalidomide has inhibiting effect on viruses of various common infectious diseases of domestic pigs at the cellular level, especially has significant inhibiting effect on proliferation of African swine fever virus (ASFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2) and porcine epidemic diarrhea virus (PEDV) in vitro. The application also discloses an antiviral composition composed of active ingredient thalidomide or pharmaceutically acceptable salts thereof and pharmaceutically acceptable carriers and / or excipients, and discloses application of the composition in preparation of viral infection inhibitors and / or in preparation of pharmaceutical preparations for preventing and / or treating viral infectious diseases, and provides a basis for development of broad-spectrum antiviral new drugs.