96 results about "Viral antibody" patented technology

The present application relates to the technical field of antibody drug determination, and provides a liquid chromatography-tandem mass spectrometry (LC-MS / MS) quantitative determination method for an anti-novel coronavirus bispecific antibody drug. According to the method, characteristic peptide segments EAYGMDVWGQGTTVTVSSASTK and SLSLSPGGGSQVQLVQSGAEVK are used, and on the basis that the characteristic peptide segments comprise a stable isotope internal standard, the content of the anti-novel coronavirus bispecific antibody drug is accurately quantified on an LC-MS / MS platform by means of the process of protein precipitation, enzymolysis and solid-phase extraction. According to the present application, the method and the characteristic peptide segments are highly selective and specific for anti-novel coronavirus bispecific antibody YBSW015, without being interfered by a pre-existing anti-novel coronavirus antibody in a patient, have the characteristics of high efficiency and simplicity, and can be used for clinical monitoring of the anti-novel coronavirus bispecific antibody.

The invention relates to a development method of an insect culture medium, which comprises the following steps: S1, constructing a double-promoter tandem vector by taking pFastBac plasmid as a skeleton; s2, establishing a baculovirus expression system; S21, transforming the double-promoter tandem vector into DH10Bac escherichia coli, so as to obtain recombinant Bacmid; s22, transfecting an Sf9 cell by using the recombinant Bacmid, so as to obtain a P0-generation baculovirus; s23, transfecting an Sf9 cell by using the P0-generation baculovirus to obtain a P1-generation baculovirus; s3, screening insect culture media: S31, providing culture media to be detected, and respectively inoculating Sf9 cells into the culture media to be detected; s32, respectively inoculating the P0-generation baculovirus or the P1-generation baculovirus into each to-be-detected culture medium for culturing; and S33, after culture is finished, detecting virus antibodies in the culture media to be detected, and screening the culture media. A comprehensive and quantitative evaluation system is established for development of the insect culture medium.

The invention discloses a system for evaluating an ADE effect and application thereof. The system comprises: (i) and (iii); (ii) and (iii); or (i), (ii) and (iii); (i) is a Middle East Respiratory Syndrome Coronavirus pseudovirus, (ii) is an antibody that targets the receptor binding domain of the Middle East Respiratory Syndrome Coronavirus, and (iii) is a cell that overexpresses CD32B; the cells are CHO cells; the pseudovirus contains a Spike protein coding gene of the Middle East Respiratory Syndrome Coronavirus. According to the invention, the ADE activity of the Middle East respiratory coronavirus antibody is identified in vitro by using MERS-CoV pseudovirus and cells for exogenous expression of CD32B. Compared with an immune cell Raji and an ADE evaluation system based on exogenous expression CD32A cells, the system provided by the invention is more sensitive, and is a potential system for evaluating the ADE effect of the Middle East respiratory coronavirus.

The invention provides a characteristic protein of serotype I chicken Marek's disease virus and detection application, and belongs to the technical field of virus detection. The invention provides a recombinant gene derived from serum I type chicken Marek's disease virus. The nucleotide sequence of the recombinant gene is shown as SEQ ID No. 1. The recombinant gene disclosed by the invention is used for coding the MDV-1 recombinant PP38 protein, and the MDV-1 recombinant PP38 protein can be applied to enzyme-linked immuno sorbent assay (ELISA) detection. The invention also provides an ELISA detection kit constructed by using the MDV-1 recombinant PP38 protein, and the ELISA detection kit can be used for clinical MDV-1 serum antibody detection, SPF chicken screening, exogenous virus antibody detection of poultry live vaccines or virus seeds, epidemiological investigation and the like, so as to prevent and control transmission of MDV-1 infection.

The invention discloses a specific monoclonal antibody for resisting a Nipah virus Post-F protein, the monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises HCDR1, HCDR2 and HCDR3, and the light chain variable region comprises LCDR1, LCDR2 and LCDR3; wherein the HCDR1, the HCDR2 and the HCDR3 respectively comprise the HCDR1, the HCDR2 and the HCDR3 in a heavy chain variable region of which the amino acid sequence is any one of SEQ ID NO: 4, 13, 20, 25, 30, 34 or 42, or respectively have at least 85% sequence identity with the HCDR1, the HCDR2 and the HCDR3; and / or the LCDR1, the LCDR2 and the LCDR3 respectively comprise LCDR1, LCDR2 and LCDR3 in a light chain variable region with the amino acid sequence of any one of SEQ ID NO: 9, 16, 23, 28, 32, 37 or 46, or respectively have at least 85% sequence identity with the LCDR1, the LCDR2 and the LCDR3. The anti-Nipah virus Post-F protein monoclonal antibody disclosed by the invention can be specifically combined with a Post-F conformation protein, and has the application potential of being developed into a Nipah virus antibody detection kit or a virus neutralizing antibody drug.

The invention relates to the technical field of immunodetection, and discloses a bovine nodular skin disease virus chimeric antigen and application thereof in an ELISA (enzyme-linked immuno sorbent assay) detection kit. According to the chimeric antigen, a gene of a dominant region of a bovine nodular skin disease virus antigen is selected to design a chimeric antigen gene, meanwhile, sequence optimization is performed, a chemical synthesis method is used for synthesizing a coding gene of the chimeric antigen of the bovine nodular skin disease virus, and then the chimeric antigen is obtained in a prokaryotic expression mode. An ELISA antibody detection kit developed on the basis of the chimeric antigen has extremely high sensitivity and specificity, can rapidly and specifically detect bovine nodular skin disease virus antibodies in serum, is suitable for monitoring and checking the nodular skin diseases of cattle in large-scale breeding, and has relatively high practical value.

The invention relates to the related technical field of detection of a porcine respiratory and reproductive disorder virus antibody, and particularly provides a recombinant antigen and an ELISA (enzyme-linked immunosorbent assay) detection kit for detecting the porcine respiratory and reproductive disorder virus antibody based on the recombinant antigen. The invention provides a recombinant antigen. The recombinant antigen is selected from a structure as shown in a formula (I), wherein the AAS1 and the AAS2 independently represent an amino acid sequence as shown in SEQ ID NO.8 or an amino acid sequence as shown in SEQ ID NO.9, and the AAS1 and the AAS2 represent different amino acid sequences; l represents an amino acid sequence # imgabs0 # in the direction from the N terminal to the C terminal, and n is selected from integers from 1 to 5. The recombinant antigen disclosed by the invention can be specifically combined with a porcine respiratory and reproductive disorder virus antibody, so that false positive detection results caused by interfering substances are greatly reduced. (1) AAS1-L-AAS2.

The invention provides a bovine akabane disease virus recombinant N protein antigen, a preparation method and an ELISA kit, relates to the field of biotechnology detection, and solves the problems that in the prior art, a competitive ELISA technical means is generally adopted in an ELISA detection method, the preparation cost is high, the price of a commercialized kit is high, and the detection cost is low. The development of beef cattle breeding enterprises in China is not facilitated. The amino acid sequence of the bovine akabane disease virus recombinant N protein antigen is as shown in SEQ ID No.2. The bovine akabane disease virus recombinant N protein antigen obtained in the invention can be specifically combined with an antibody of bovine akabane disease virus through verification, and can be used as a candidate antigen for bovine akabane disease virus detection; the ELISA detection kit developed by the invention can be used for detecting the bovine akabane disease virus antibody, and has the advantages of strong specificity, high sensitivity, good repeatability, low cost, large-scale detection and the like.

The invention discloses an Olvapu virus fusion protein, which is formed by connecting and fusing an Olvapu virus Gn protein, an Olvapu virus Gc protein and an Olvapu virus N protein by using GSGSG, and the fusion protein comprises an amino acid sequence as shown in SEQ ID NO.1. The invention further discloses a preparation method of the Olvapu virus fusion protein. The invention also discloses a nucleotide sequence corresponding to the fusion protein, construction of the gene expression vector, a host cell, and a preparation method and application of the fusion protein. Colloidal gold detection test paper for detecting the Oroptovirus antibody in a human body is researched and developed by utilizing the fusion protein, and the test paper has the advantages of high sensitivity, good specificity and high detection accuracy, does not need expensive instruments and equipment at an application end, and has huge clinical significance and wide application prospects.

The utility model discloses a rubella virus IGG antibody detection kit, and relates to the technical field of kits, in particular to a rubella virus IGG antibody detection kit which comprises a kit body, an adjusting sliding groove is formed in the inner bottom wall of the kit body, a supporting sliding rod is arranged in the adjusting sliding groove, the outer surface of the supporting sliding rod is connected with a connecting sliding block in a sliding mode, and the connecting sliding block is arranged in the adjusting sliding groove. And connecting springs are arranged on the outer surfaces of the connecting sliding blocks. According to the rubella virus IGG antibody detection kit, supporting sliding rods, connecting sliding blocks, connecting springs, a fixing frame, clamping plates, first springs and spring dampers are arranged in a matched mode, in the using process, after a reagent penetrates through a protective baffle, the clamping plates are extruded, the first springs are extruded, the distance between the clamping plates is adjusted, the reagent is inserted into the clamping plates, the clamping plates are clamped, the clamping plates are clamped, and the clamping plates are clamped. Meanwhile, the lower end of the kit is propped against the soft rubber protective pad, so that the rubella virus IGG antibody detection kit has the effects of conveniently and quickly fixing the reagent and improving the shock resistance.

The invention provides a joint detection test strip for dengue virus and Zika virus antigens and antibodies and a preparation method of the joint detection test strip. According to the combined detection test strip, the combined detection test strip with the dengue virus NS1 antigen and the dengue virus IgG / IgM antibody adopts a multi-site recombinant antigen labeling or multi-site recombinant antibody coating form, so that the sensitivity of a reagent is improved; the problems of poor specificity and release residue of latex microspheres are solved by optimizing the confining liquid; colored latex microspheres are used as a marker, a detection target is more intuitively distinguished according to line colors, clinical result interpretation is facilitated, the accuracy of dengue virus infection and Zika virus infection is more quickly improved, and the dengue virus NS1, the Zika virus NS1 antigen, the dengue virus IgG / IgM antibody and the Zika virus IgG / IgM antibody can be detected at the same time; and the kit is convenient to carry, simple to operate, convenient for on-site instant detection and capable of performing large-scale preliminary screening.

Provided herein are anti-adeno-associated virus (AAV) antibodies and antigen fragments thereof, as well as multispecific antibodies (e.g., bispecific antibodies) that bind to both a capsid of an AAV particle and a molecule on a cell surface, related molecular complexes, affinity matrices, and pharmaceutical compositions, and methods of use thereof.

The application discloses a kit for detecting equine infectious anemia virus antibody and belongs to the field of animal virus antibody detection. The kit comprises equine infectious anemia virus p26-gp90 recombinant protein, mouse IgG and goat anti-mouse IgG, and the equine infectious anemia virus p26-gp90 recombinant protein is composed of an amino acid sequence shown in SEQ ID NO. 1. The kit is used for detecting equine infectious anemia virus antibody, is convenient and fast, has high sensitivity, has no cross reaction with other pathogens, has high specificity, has great clinical significance and wide application prospect.

Disclosed herein are methods for inducing immunity against a virus such as a coronavirus in the mucosal tissue of a patient, include administering a vaccine composition to the patient by oral administration (e.g., nasal injection, nasal inhalation, oral inhalation, and / or oral ingestion). Also disclosed are compositions for assaying the presence of anti-viral antibodies induced by the administered vaccine or the presence of viral proteins in a saliva sample include a stabilizing solution and may also include the use of aragonite particle beads. Compositions and methods are presented for prevention and / or treatment of a coronavirus disease wherein the composition comprises a recombinant entity. The recombinant entity is bivalent, comprising a nucleic acid encoding a coronavirus 2 nucleocapsid protein CoV2 nucleocapsid protein fused to an endosomal targeting sequence, and a nucleic acid encoding a CoV2 spike protein sequence optimized for cell surface expression.

The invention relates to methods and reagents for analyzing samples for the presence of JC virus antibodies.

The application relates to the field of rapid detection of new coronaviruses, in particular to a single-layer MoS2 phase sensor detection box for a new coronavirus vaccine antigen protein and a detection method thereof. The process that a MoS2 test sample is combined with a standard new coronavirus SARS-CoV-2 antibody is as follows: firstly, single-layer MoS2 is grown through a CVD method; further, ZnO is deposited on SiO2 / Si through atomic layer deposition (ALD); the single-layer MoS2 is transferred to a substrate through PMMA (polymethyl methacrylate) assisted wet transfer; the surface of the MoS2 is subjected to surface treatment; and the problem that current detection methods can only qualitatively detect whether there is a new coronavirus and cannot measure a specific concentration is solved. On the premise of ensuring high sensitivity, the actual concentration of a sample can be quantitatively measured. The pretreatment process of the sample is simple, the detection process is short in time consumption, the measurement result is intuitive and accurate, and the application has important significance for quantitative detection of the concentration of a new coronavirus for a large number of samples.

The utility model relates to the technical field of hog cholera virus antibody detection, in particular to a hog cholera virus antibody detection device, which is provided with a sealing mechanism and a self-locking mechanism, so that in the hog cholera virus antibody detection process, the shaking of a test tube can be reduced when raw materials are stored, the raw materials are prevented from overflowing and being doped, and the detection result is not accurate. Comprising a storage mechanism; the device further comprises a heat preservation mechanism, a plurality of supporting mechanisms, a plurality of sealing mechanisms and a plurality of self-locking mechanisms, the heat preservation mechanism is installed at the lower end of the storage mechanism, the supporting mechanisms are installed in the storage mechanism, the sealing mechanisms are installed in the storage mechanism, and the self-locking mechanisms are installed in the storage mechanism. The storage mechanism is used for storage, the heat preservation mechanism is used for heat preservation, the supporting mechanism is used for storage, the sealing mechanism is used for sealing, and the self-locking mechanism is used for self-locking.

This invention relates to the field of biotechnology, and in particular to an antigenic epitope peptide, product, and application for detecting antibodies produced by porcine deltacoronavirus infection. This invention identified short peptide epitopes in PDCoV longitudinal challenge serum samples, obtaining 12 short peptides, which were then prepared into a porcine deltacoronavirus antibody detection kit. Finally, six field serum samples were selected for antibody detection, and the effectiveness was verified using a virus neutralization experiment. The results showed that the porcine deltacoronavirus antibody detection kit provided by this invention is effective. Furthermore, the porcine deltacoronavirus antibody detection kit provided by this invention, as a novel detection method, improves sensitivity and specificity while simultaneously providing multiple detection results, namely, determining whether viral infection has occurred, identifying the stage of infection, and achieving DIVA (Differentiation, Infection, and Variability) analysis.

The present invention discloses a system for evaluating the ADE effect and its application. The system comprises: (i) and (iii); (ii) and (iii); or (i), (ii) and (iii); (i) is a pseudovirus of Middle East respiratory syndrome coronavirus, (ii) is an antibody targeting the receptor binding domain of Middle East respiratory syndrome coronavirus, and (iii) is a cell overexpressing CD32B; the cell is a CHO cell; the pseudovirus comprises the Spike protein encoding gene of Middle East respiratory syndrome coronavirus. The present invention utilizes MERS-CoV pseudovirus and cells exogenously expressing CD32B to identify the ADE activity of Middle East respiratory coronavirus antibodies in vitro. The system provided by the present invention is more sensitive than the ADE evaluation system based on immune cell Raji and cells exogenously expressing CD32A, and is a potential system for evaluating the ADE effect of Middle East respiratory coronavirus.

The utility model relates to the technical field of detection kits, in particular to a virus antibody detection kit which comprises a virus antibody detection kit body, an edge groove is formed in one end of the virus antibody detection kit body, a reagent dropping area is arranged on the top surface of the edge groove, and a medical silica gel cover is fixedly sleeved on the surface of the edge groove. The medical silica gel cover covers the reagent dropping area, a notch is formed in the surface of the virus antibody detection kit body, a transparent sealing sheet is fixed on the surface of the notch, and a marking paper strip is inserted between the notch and the transparent sealing sheet; the virus antibody detection kit provided by the utility model has the beneficial effects that the medical silica gel cover is arranged at the end part of the kit body, and the silica gel patch matched with the notch of the reagent dropping area is arranged at the bottom of the silica gel cover, so that the reagent dropping area is effectively protected in an unused state, external pollution and damage are avoided, and the service life of the reagent dropping area is prolonged. Therefore, the accuracy of the detection result is improved.

The invention discloses a rabies virus antibody detection device, and belongs to the technical field of antibody detection.The rabies virus antibody detection device comprises a base, a lower cylinder located on the base and an upper cylinder located on the lower cylinder, the lower cylinder is of a cylindrical cavity structure with an opening in the top, and the upper cylinder is of a cylindrical cavity structure with openings in the top and the bottom; a centrifugal mechanism is arranged in the lower cylinder body, a plurality of mounting holes are formed in the side wall of the lower cylinder body in the circumferential direction, test paper placing blocks are movably arranged in the mounting holes, a plurality of magnetic blocks are fixed to the top wall of the upper cylinder body, adsorption rods are adsorbed to the bottoms of the magnetic blocks, a movable plate is movably arranged in the upper cylinder body, and the movable plate is fixed to the upper cylinder body. One end of the adsorption rod is fixedly connected with the movable plate; according to the invention, the blood sample of the testee can be rapidly detected, the test result can be obtained within half an hour to one hour, and the test result is accurate, convenient and rapid.

The invention belongs to the technical field of biology, and particularly relates to a Prussian blue nano test strip for detecting an African horse pestivirus antibody as well as a preparation method and application of the Prussian blue nano test strip. A sample pad, a combination pad, a chromatography detection membrane and a water absorption pad are sequentially fixed on the supporting bottom plate; the chromatography detection membrane is provided with a detection line and a quality control line, the detection line is coated with staphylococcus A protein, and the quality control line is coated with a monoclonal antibody of African horse pestivirus VP7 recombinant protein; and the combination pad is coated with an African horse pestivirus VP7 delta N1-129 recombinant protein fragment coupled with Prussian blue nanoparticles. According to the invention, Prussian blue nano is used as a coupling tracer, and the specific reaction of an antigen and an antibody is fully utilized to detect the African horse pestivirus antibody. After the conjugate is combined with a corresponding antibody, a result can be clearly observed, and the conjugate is good in stability, high in sensitivity and strong in specificity, has a clinical application function and huge development potential, and is an extremely ideal antibody test strip detection material.

The application provides a combined detection test strip for dengue virus, Zika virus antigen and antibody and a preparation method thereof; the combined detection test strip of the application adopts a multi-site recombinant antigen marking or multi-site recombinant antibody coating form for the combined detection test strip for dengue virus NS1 antigen and dengue virus IgG / IgM antibody, thereby improving the sensitivity of the reagent; the application also overcomes the problems of poor specificity and release residue of the latex microspheres by optimizing a blocking solution; color latex microspheres are used as the markers, the target is more intuitively detected according to the color of the lines, clinical result interpretation is facilitated, the accuracy of dengue virus infection and Zika virus infection is improved, dengue virus NS1, Zika virus NS1 antigen, dengue virus IgG / IgM antibody detection and Zika virus IgG / IgM antibody can be simultaneously detected; moreover, the application is convenient to carry and easy to operate, is convenient for on-site instant detection and can be used for large-scale preliminary screening.

The invention discloses a polypeptide-based avian reticuloendotheliosis virus indirect ELISA antibody detection method, and belongs to the technical field of biology. According to the kit, potential epitopes of REV gp90 protein are systematically screened to obtain three sections of specific polypeptide antigens for detecting the avian reticuloendotheliosis virus antibody, and a mixed product of the three sections of specific polypeptide antigens is used as a coating antigen to develop the indirect ELISA antibody detection kit for the avian reticuloendotheliosis virus. On the basis of the indirect ELISA antibody detection kit, the avian reticuloendotheliosis virus antibody detection method established by optimizing the coating concentration, the sealing condition, the serum incubation time and the like has the advantages of strong specificity, high sensitivity, good repeatability and convenience in operation; the kit can effectively and rapidly diagnose epidemic diseases caused by the avian reticuloendotheliosis virus, and is beneficial to popularization and application.

The application discloses an antibody against syncytial virus and application thereof, and relates to the field of antibodies. The antibody against syncytial virus disclosed by the application comprises a heavy chain complementarity determining region and a light chain complementarity determining region, the antibody provides an important raw material source for detection of syncytial virus, and has good activity and sensitivity.

The invention belongs to the technical field of biological medicine, and discloses a novel pathogen attenuated live vaccine and application thereof. The invention discloses an attenuated live vaccine, which comprises at least one pathogenic microorganism and a neutralizing antibody capable of carrying out a neutralizing reaction with the pathogenic microorganism, and provides a virus-antibody compound formed by combining the at least one pathogenic microorganism with the neutralizing antibody having a neutralizing capability on the pathogenic microorganism, the attenuated live vaccine, namely an attenuated live vaccine (such as an influenza attenuated live vaccine), has relatively good safety, immunogenicity and protectiveness, and has a better prevention or improvement effect compared with a traditional inactivated vaccine. Meanwhile, the preparation method of the attenuated live vaccine is simple, and compared with the traditional attenuated live vaccine, the attenuated live vaccine has the characteristics of quicker speed (the preparation time is about 1 hour) and easiness in preparation.

The present disclosure is directed to antibodies binding to and neutralizing henipavirus and methods for use thereof. Thus, in accordance with the present disclosure, there is provided a method of detecting a henipavirus infection in a subject comprising (a) contacting a sample from said subject with an antibody or antibody fragment having clone-paired heavy and light chain CDR sequences from Tables 3 and 4, respectively; and (b) detecting henipavirus in said sample by binding of said antibody or antibody fragment to a henipavirus antigen in said sample.

This disclosure belongs to the field of biotechnology, specifically relating to anti-P[8] genotype rotavirus antibodies or their antigen-binding fragments, their preparation methods, and applications. The antibody or its antigen-binding fragment can specifically recognize and bind to the VP8 protein of P[8] genotype rotavirus, and can be used for the diagnosis, prevention, and / or treatment of P[8] genotype rotavirus infection or diseases caused by it, the detection of the presence or level of P[8] genotype rotavirus, its VP8 protein, or fusion proteins containing its VP8 protein in samples, or the development or screening of drugs for the prevention and / or treatment of P[8] genotype rotavirus infection or diseases caused by it.

The invention belongs to the technical field of veterinary diagnosis, and particularly relates to a novel duck reovirus agar diffusion antigen as well as a preparation method and application thereof. The preparation method comprises the following steps: inoculating a novel duck reovirus GX2022 strain to an SPF (specific pathogen free) duck embryo at the age of 6-10 days, harvesting and inoculating a diseased duck embryo body within 24-144 hours, adding sterile normal saline, fully grinding, freezing and thawing (freezing at the temperature of-20 DEG C and thawing at the temperature of 25 DEG C), carrying out solid-liquid separation, collecting supernate, adding formaldehyde for inactivation, uniformly mixing the inactivated virus liquid with a freeze-drying protective additive, and freeze-drying to obtain the agar diffusion antigen. Compared with the prior art, the method has the advantages that the agar diffusion titer is as high as 1: 24, the specificity is strong, the stability is good, the titer is high, the yield is high, the reaction precipitation line is clear, the detection is convenient, the method can be used for evaluating the immune effect of the NDRV and diagnosing the infection of the NDRV, can also be used for screening NDRV antibody negative ducks, detecting exogenous virus antibodies of waterfowl live vaccines or virus seeds, performing epidemiological investigation and the like, and has a wide application prospect.

The invention belongs to the technical field of biology, and particularly relates to an anti-dengue virus antibody or an antigen binding fragment thereof as well as a preparation method and application thereof. The antibody or the antigen binding fragment thereof can specifically recognize and bind various serotype dengue viruses or E proteins thereof, and has good affinity with the various serotype dengue viruses or E proteins thereof; meanwhile, the compound has relatively good neutralizing activity on various serotype dengue viruses; the polypeptide can be used for preparing products for diagnosing, preventing and / or treating dengue virus infection or diseases caused by the dengue virus infection, detecting existence or level of the dengue virus or E protein of the dengue virus in a sample or screening drugs for preventing and / or treating the dengue virus infection or the diseases caused by the dengue virus infection.