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ELISA (enzyme linked immunosorbent assay) antibody detection kit for O type foot-and-mouth disease virus and detection method adopting ELISA antibody detection kit

A technology for antibody detection and detection methods, applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as slowing down the speed of experimental detection

Inactive Publication Date: 2015-11-18
LUOYANG LAIPSON INFORMATION TECH
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The virus neutralization test established in the 1970s is a relatively classic serological detection method, but the in vitro VNT test requires monolayer cell culture, which has great limitations
However, it requires multiple washing processes, which greatly slows down the speed of experimental detection.

Method used

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  • ELISA (enzyme linked immunosorbent assay) antibody detection kit for O type foot-and-mouth disease virus and detection method adopting ELISA antibody detection kit
  • ELISA (enzyme linked immunosorbent assay) antibody detection kit for O type foot-and-mouth disease virus and detection method adopting ELISA antibody detection kit
  • ELISA (enzyme linked immunosorbent assay) antibody detection kit for O type foot-and-mouth disease virus and detection method adopting ELISA antibody detection kit

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Embodiment Construction

[0036] The present invention will be further described below in conjunction with accompanying drawing and embodiment, the method in the embodiment, if no special instructions is conventional method:

[0037] A foot-and-mouth disease type O virus ELISA antibody detection kit comprises the following components:

[0038] (1) Elisa plate coated with recombinant RE2 protein antigen; (2) horseradish peroxidase-labeled monoclonal antibody; (3) negative control; (4) positive control; (5) washing solution; (6) sample Diluent; (7) Chromogenic Solution A and Chromogenic Solution B; (8) Stop Solution;

[0039] RE2 recombinant protein primer synthesis and protein expression

[0040] The recombinant RE2 protein is a multi-epitope gene recombinant RE2 protein including porcine foot-and-mouth disease O-type VP1 protein synthesized by genetic engineering technology;

[0041] According to foot-and-mouth disease O / HK / 02 gene sequence on GenBank, design 1 pair of expression primers, the prime...

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Abstract

The invention relates to an ELISA (enzyme linked immunosorbent assay) antibody detection kit for an O type foot-and-mouth disease virus and a detection method adopting the ELISA antibody detection kit. The kit comprises components as follows: (1) a recombinant RE2 protein antigen coated Elisa plate, (2) a horseradish peroxidase marked monoclonal antibody, (3) negative control, (4) positive control, (5) a washing solution, (6) a sample diluent, (7) a developing solution A and a developing solution B and (8) a stop solution. The detection kit combines a monoclonal antibody technology and a solid-phase ELISA detection technology, has the characteristics of rapidness, accuracy, stability and the like, and can be used for immune effect monitoring of a livestock farm as well as rapid and batch antibody detection of epidemic diseases of an inspection and quarantine department, an export port and the like.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a foot-and-mouth disease O-type virus ELISA antibody detection kit and a detection method thereof. Background technique [0002] Foot-and-mouth disease is a highly contagious cloven-hoofed animal disease caused by foot-and-mouth disease virus. Due to its strong transmission and high incidence, it seriously affects the development of animal husbandry in various countries. At the same time, it is also a zoonotic disease, which is extremely harmful to society. Has been classified as a class of infectious diseases by the Ministry of Agriculture. According to the serotype of FMD, it is divided into seven types: A, O, C, AsiaⅠ, SATⅠ, SATⅡ, and SATⅢ. Each type has several subtypes, and the antigenicity between them is very different. It is necessary for the monitoring of FMD. The prevention and control work has brought many difficulties, and the establishment of a fast, ...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68G01N2333/09
Inventor 李秀梅闫若潜岳华张小莺杨志谢彩华方先珍刘玉宝刘梅芳
Owner LUOYANG LAIPSON INFORMATION TECH
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