530 results about "Antibody detection" patented technology

The invention belongs to the technical field of medicines, and particularly relates to application of circRNA (Ribonucleic Acid) coded protein as a gastric cancer marker. The circRNA disclosed by the invention is provided with an ORF (Open Reading Frame) capable of coding a microprotein circUBE2G1-99aa of 99 amino acids, and the amino acid sequence of the protein is shown as SEQ ID NO: 1. According to the present invention, the designed circUBE2G1-99aa targeting antibody is adopted to detect the expression level of circUBE2G1-99aa in the collected clinical sample, and the expression level of circUBE2G1-99aa in the stomach cancer patient tissue is down-regulated, and is negatively correlated with the large tumor, the lymph node metastasis and the advanced pathological stage of the stomach cancer patient.

The application belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody pair for detecting feline immunodeficiency virus p24 protein and application thereof. The monoclonal antibody pair is monoclonal antibody 1D8 and monoclonal antibody 1H10, which can specifically recognize feline immunodeficiency virus p24 protein, and the heavy chain and light chain variable region complementarity determining region sequences of 1D8 and 1H10 are clear, and are respectively shown as SEQ ID NO. 1-12. The antibody pair can effectively avoid cross reaction, and significantly improve the accuracy and sensitivity of detection; the colloidal gold detection test strip constructed based on the antibody pair is simple and fast in operation, is suitable for early antigen detection of FIV infection, solves the problem that existing antibody detection cannot distinguish natural infection and vaccine immunization, and provides a reliable technical means for on-site screening and disease prevention and control of FIV.

The invention discloses a novel coronavirus neutralizing antibody, a detection kit and application of the novel coronavirus neutralizing antibody. The amino acid sequence of a heavy chain variable region of the neutralizing antibody is shown as SEQ ID No.1, and the amino acid sequence of a light chain variable region of the neutralizing antibody is shown as SEQ ID No.2. The antibody with mature affinity is screened through bioinformatics analysis of a single B cell, the antibody screening process is optimized by combining single cell RNA sequencing, VDJ rearrangement analysis and somatic cell hypermutation research, blindness of a traditional method is avoided, and the accuracy and effectiveness of antibody screening are improved. According to the neutralizing antibody GR12 provided by the invention, a heavy chain variable region and a light chain variable region of the neutralizing antibody GR12 can be specifically combined with an RBD structural domain of the SARS-CoV-2 and an S-Trimer structural domain of an Omicro variant, so that a broad-spectrum neutralizing effect on the SARS-CoV-2 virus and the variant thereof is realized. The binding activity of the antibody GR12 to S-Trimer and RBD under 2-fold and 300-fold dilution conditions is obviously superior to that of other antibodies, which indicates that the antibody GR12 has high affinity and dilution stability and is suitable for clinical large-dose administration.

The invention relates to the technical fields of gene engineering, antibody engineering and immunodiagnosis. The invention provides a recombinant humanized anti-Cpn IgM monoclonal antibody. The monoclonal antibody comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises three complementary determining regions CDR1, CDR2 and CDR3, and the amino acid sequences of the three complementary determining regions are respectively shown as SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5; the heavy chain variable region comprises three complementary determining regions CDR1, CDR2 and CDR3, and the amino acid sequences of the three complementary determining regions are shown as SEQ ID NO.6, SEQ ID NO.7 and SEQ ID NO.8 respectively. The invention also provides a method for preparing the monoclonal antibody. The recombinant humanized anti-Cpn IgM monoclonal antibody provided by the invention is expressed in mammalian cells by utilizing a gene recombination technology, has the characteristics of high purity, small batch difference and the like, and can be applied to a Cpn IgM antibody detection kit as a quality control product; as a quality control product, the recombinant humanized anti-Cpn IgM monoclonal antibody has the advantages of high sensitivity, high safety, inter-batch stability and the like.

The invention discloses a novel coronavirus neutralizing antibody, a detection kit and application of the novel coronavirus neutralizing antibody. The amino acid sequence of a heavy chain variable region of the neutralizing antibody is shown as SEQ ID No.1, and the amino acid sequence of a light chain variable region of the neutralizing antibody is shown as SEQ ID No.2. The antibody with mature affinity is screened through bioinformatics analysis of a single B cell, the antibody screening process is optimized by combining single cell RNA sequencing, VDJ rearrangement analysis and somatic cell hypermutation research, blindness of a traditional method is avoided, and the accuracy and effectiveness of antibody screening are improved. According to the neutralizing antibody GR75 provided by the invention, a heavy chain variable region and a light chain variable region of the neutralizing antibody GR75 can be specifically combined with an RBD structural domain of the SARS-CoV-2 and an S-Trimer structural domain of an Omicro variant, so that a broad-spectrum neutralizing effect on the SARS-CoV-2 virus and the variant thereof is realized. The binding activity of the antibody GR75 to S-Trimer and RBD under 2-fold and 300-fold dilution conditions is obviously superior to that of other antibodies, which indicates that the antibody GR75 has high affinity and dilution stability and is suitable for clinical large-dose administration.

The invention discloses a novel coronavirus neutralizing antibody GR46, a detection kit and application of the novel coronavirus neutralizing antibody GR46. The amino acid sequence of a heavy chain variable region of the neutralizing antibody is shown as SEQ ID No.1, and the amino acid sequence of a light chain variable region of the neutralizing antibody is shown as SEQ ID No.2. The antibody with mature affinity is screened through bioinformatics analysis of a single B cell, the antibody screening process is optimized by combining single cell RNA sequencing, VDJ rearrangement analysis and somatic cell hypermutation research, blindness of a traditional method is avoided, and the accuracy and effectiveness of antibody screening are improved. According to the neutralizing antibody GR46 provided by the invention, a heavy chain variable region and a light chain variable region of the neutralizing antibody GR46 can be specifically combined with an RBD structural domain of the SARS-CoV-2 and an S-Trimer structural domain of an Omicro variant, so that a broad-spectrum neutralizing effect on the SARS-CoV-2 virus and the variant thereof is realized. The binding activity of the antibody GR46 to S-Trimer and RBD under 2-time and 300-time dilution conditions is obviously superior to that of other antibodies, which indicates that the antibody GR46 has high affinity and dilution stability and is suitable for clinical large-dose administration.

The invention provides a characteristic protein of serotype I chicken Marek's disease virus and detection application, and belongs to the technical field of virus detection. The invention provides a recombinant gene derived from serum I type chicken Marek's disease virus. The nucleotide sequence of the recombinant gene is shown as SEQ ID No. 1. The recombinant gene disclosed by the invention is used for coding the MDV-1 recombinant PP38 protein, and the MDV-1 recombinant PP38 protein can be applied to enzyme-linked immuno sorbent assay (ELISA) detection. The invention also provides an ELISA detection kit constructed by using the MDV-1 recombinant PP38 protein, and the ELISA detection kit can be used for clinical MDV-1 serum antibody detection, SPF chicken screening, exogenous virus antibody detection of poultry live vaccines or virus seeds, epidemiological investigation and the like, so as to prevent and control transmission of MDV-1 infection.

The invention discloses a porcine delta coronavirus monoclonal antibody and application thereof in preparation of an antigen detection test strip. The detection test strip comprises a polyvinyl chloride bottom plate on the lowest layer, and a sample pad, a conjugate pad, a nitrocellulose membrane and a water absorption pad are sequentially laid on the polyvinyl chloride bottom plate in the flowing direction; the sample pad is partially overlapped on the combination pad, and the combination pad and the water absorption pad are respectively overlapped on two sides of the nitrocellulose membrane; a detection line and a quality control line are arranged on the nitrocellulose membrane along the flowing direction; an antibody marked by red latex microspheres is sprayed on the combination pad, a coated antibody is sprayed on the detection line, and coated commercial goat anti-mouse IgG is sprayed on the quality control line. The test strip adopts a double-antibody sandwich method for detection, and has the advantages of high detection speed, high sensitivity, strong specificity and the like. The detection result is visual, clear and easy to interpret; the detection process does not need any instrument and equipment, operators do not need professional training, operation is easy and convenient, and the method is particularly suitable for on-site rapid diagnosis.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody pair for detecting cat immunodeficiency virus p24 protein and application of the monoclonal antibody pair. The monoclonal antibody pair is a monoclonal antibody 1D8 and a monoclonal antibody 1H10 and can specifically recognize cat immunodeficiency virus p24 protein, and sequences of complementary determining regions of variable regions of a heavy chain and a light chain of the 1D8 and the 1H10 are clear and are respectively shown as SEQ ID NO.1-12. The antibody pair can effectively avoid cross reaction, and the accuracy and sensitivity of detection are remarkably improved; the colloidal gold test strip constructed on the basis of the antibody pair is simple, convenient and rapid to operate, is suitable for early antigen detection of FIV infection, solves the problem that natural infection and vaccine immunity cannot be distinguished in existing antibody detection, and provides a reliable technical means for FIV field screening and disease prevention and control.

The invention relates to the technical field of antibodies, in particular to an anti-African swine fever virus p30 protein monoclonal antibody and application thereof, and the anti-African swine fever virus p30 protein monoclonal antibody can be specifically combined with African swine fever virus p30 protein. The anti-ASFV p30 protein monoclonal antibody is prepared by immunizing a BALB / c mouse through an immunological method, and the antibody is high in specificity, titer and affinity and can be applied to immunological detection or antibody detection kits.

The invention relates to the technical field of antibody detection methods, in particular to a diagnosis method and a detection system based on autoimmune disease antibody detection, and the method comprises the following steps: S1, an analysis module obtains electrochemistry related information and light reflection related information through electrochemical analysis and light reflection analysis; related information of electrochemistry and related information of light reflection are transmitted to the control module; s2, the control module obtains an antibody concentration index according to the electrochemistry related information and the light reflection related information, and transmits the antibody concentration index to a diagnosis module; s3, the diagnosis module obtains strong or weak immunoreaction information according to the antibody concentration index, and transmits the strong or weak immunoreaction information to the communication module; and S4, the communication module transmits strong or weak immunoreaction information to the user side. Immunoassay is combined with electrochemical analysis and light reflection analysis for detection, and the accuracy and comprehensiveness of detection can be improved by analyzing in different directions and combining results.

The invention discloses an antibody for detecting indol sulfate, a detection kit and application of the antibody, and the antibody can be specifically combined with indol sulfate and has high combination activity. The detection kit detects the content of indoxyl sulfate in a to-be-detected sample through a competitive binding reaction of an immunomagnetic bead coated indoxyl sulfate antibody, an antigen in the sample and an alkaline phosphatase labeled indoxyl sulfate antigen, and has important economic value and social significance.

The invention discloses a monoclonal antibody, a hybridoma cell secreting the monoclonal antibody and application of the hybridoma cell, and belongs to the field of peptides. The invention aims to provide a monoclonal antibody and a hybridoma cell secreting the monoclonal antibody. The hybridoma cell secreting the monoclonal antibody disclosed by the invention is a hybridoma cell 3G3, and the preservation number of the hybridoma cell 3G3 in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.46345. The monoclonal antibody and the BVDV antibody detection kit based on the monoclonal antibody and the blocking ELISA method can be used for detecting a neutralizing antibody of the BVDV, and have the characteristics of strong specificity, good sensitivity, high sensitivity, high accuracy and the like.

The invention discloses a lateral flow chromatography test strip for detecting perfluorooctanoic acid by combining gold nanoparticles, a nucleic acid aptamer and an antibody. According to the test strip, a nucleic acid aptamer marked by gold nanoparticles is combined with PFOA molecules to form a nucleic acid aptamer with a G-quadruplex structure, and then the nucleic acid aptamer with the G-quadruplex structure is specifically recognized and captured by an anti-BG4 antibody, so that specific visual detection of perfluorooctanoic acid is realized. After a sample is injected, a G-quadruplex structure formed by the PFOA and the nucleic acid aptamer acts with the anti-BG4 antibody coated on the test strip, so that the gold nanoparticles are agglomerated to generate a chromogenic reaction, the signal intensity can be detected by naked eyes or reading equipment, the visual detection limit is 10 mu M, and the equipment detection limit is 304 nM. The test strip has the advantages of high detection speed, high specificity and high sensitivity, and is suitable for field detection and rapid screening. Compared with a traditional method, the method realizes simple, convenient and economical detection of PFOA, and has a wide application prospect.

The invention provides a CuCoNi-MOF aerogel nano-enzyme, a preparation method thereof and a detection reagent, and belongs to the technical field of antibody detection.The preparation method of the aerogel nano-enzyme comprises the following steps that bivalent copper salt, bivalent cobalt salt and bivalent nickel salt are mixed, then a reducing agent is added to reduce bivalent metal ions, then a dimethylimidazole solution is added, and the CuCoNi-MOF aerogel nano-enzyme is obtained. And reacting at 25-35 DEG C for at least 1 hour, finally taking out the precipitate after the reaction, washing with deionized water, and freeze-drying to obtain the target product. The detection reagent provided by the invention is prepared from the aerogel nano-enzyme as a raw material. The aerogel nano enzyme has high activity and strong stability, and can form a detection reagent with stable properties with a protein biomarker, and meanwhile, the preparation method is simple and convenient, and does not need expensive instruments, so that the manufacturing cost can be reduced. The detection reagent provided by the invention has very high sensitivity and accuracy.

The invention relates to a layered prediction method and system for the risk of malignant tumors related to dermatomyositis. The method comprises the following steps: collecting clinical index data of a patient suffering from dermatomyositis, the clinical index data comprising an anti-TIF1-gamma antibody detection result, an interstitial lung disease existence state, a dermatomyosis existence state, an anemia state and a dermatomyositis type; constructing a comprehensive scoring model to generate a risk stratification prediction result according to the clinical index data; the comprehensive scoring model is used for performing binary assignment on each index to obtain a score of a corresponding item; calculating the sum of scores of all indexes of the patient to obtain a total risk score; and layering the risk of the malignant tumor accompanied by the patient based on the total risk score, and outputting a risk layering result. According to the method, the cancer risk of the patient with dermatomyositis can be effectively predicted, and the reliability, convenience and practicability of layered prediction of the cancer risk are improved.

The invention discloses an anti-soluble transferrin receptor monoclonal antibody as well as a preparation method and application thereof, and belongs to the technical field of antibody detection. According to the antibody, an L101-F760 amino acid sequence of human TfR protein is used as an immunogen, specific screening of B cells, reverse RNA (Ribonucleic Acid) into cDNA (Complementary Deoxyribose Nucleic Acid) through RT-PCR (Reverse Transcription-Polymerase Chain Reaction), then homologous recombination is performed to construct heavy and light chain vectors, and finally multiple rounds of screening are performed to obtain the purified sTfR monoclonal antibody, so that the sTfR protein can be specifically recognized, and the antibody has the advantages of high specificity and high sensitivity and can be widely applied to the field of immunotherapy. The natural sTfR protein in human serum can be recognized. The kit is applied to the detection and screening fields of immunohistochemistry, immunofluorescence chemistry, western blot, co-immunoprecipitation, indirect ELISA, flow cytometry and the like, especially has low background and no interference of non-specific binding impure protein during immunohistochemistry detection, can obtain more accurate detection and evaluation results, avoids interference of false positive and false negative, and has high detection accuracy. The method has great application value in the aspects of basic scientific research and clinical pathology detection.

The invention relates to the technical field of immunodetection, and discloses a bovine nodular skin disease virus chimeric antigen and application thereof in an ELISA (enzyme-linked immuno sorbent assay) detection kit. According to the chimeric antigen, a gene of a dominant region of a bovine nodular skin disease virus antigen is selected to design a chimeric antigen gene, meanwhile, sequence optimization is performed, a chemical synthesis method is used for synthesizing a coding gene of the chimeric antigen of the bovine nodular skin disease virus, and then the chimeric antigen is obtained in a prokaryotic expression mode. An ELISA antibody detection kit developed on the basis of the chimeric antigen has extremely high sensitivity and specificity, can rapidly and specifically detect bovine nodular skin disease virus antibodies in serum, is suitable for monitoring and checking the nodular skin diseases of cattle in large-scale breeding, and has relatively high practical value.

The invention discloses an indirect ELISA (enzyme-linked immuno sorbent assay) detection method of a porcine epidemic diarrhea virus antibody SIgA, and belongs to the technical field of animal antibody detection. In order to solve the problems of limited detection range and single immune evaluation dimension of a porcine epidemic diarrhea virus detection method in the prior art, the invention provides an indirect ELISA detection method of a porcine epidemic diarrhea virus antibody SIgA, and the detection method adopts a pichia pastoris eukaryotic secretion platform to prepare a high-activity PEDV S1 antigen; meanwhile, a secreting type IgA antibody is used as a detection target; the method has the advantages of high detection sensitivity, low background noise, better variant compatibility and practical popularization value, and provides a clinical and industrial-level solution for maternal immunity monitoring, piglet passive protection evaluation and vaccine immunity strategy optimization.

The invention relates to the technical field of infectious disease detection, and discloses a new respiratory infectious disease early warning method, which comprises the following steps: S1, specimen collection and data collection: collecting biological samples of new respiratory infectious disease virus infected individuals and other respiratory infectious disease virus infected individuals, the biological sample comprises a blood sample and a swab sample; meanwhile, basic information of the individuals is collected, wherein the basic information comprises gender, age and respiratory symptom duration; s2, quantitative detection: the biological sample is subjected to quantitative detection by adopting reagents of various respiratory infectious disease viruses, the detection content comprises antigens and antibodies, and the antibodies comprise an I gM antibody, an I gG antibody and an I gA antibody; according to the invention, basic information of individuals and antigen-antibody detection reagent resources of various respiratory infectious disease viruses are deeply integrated, so that the recognition capability of new respiratory infectious disease virus infected individuals is effectively improved.

The invention provides an autoimmune disease antibody detection system based on a biosensing technology, which is characterized by comprising a data acquisition module, a data processing module, a detection analysis module and an interaction management module, the data acquisition module is used for acquiring comprehensive detection information of the autoimmune disease of a patient, the data processing module is used for preprocessing the comprehensive detection information, and the detection analysis module is used for analyzing and evaluating the autoimmune disease risk of the patient in combination with the preprocessed comprehensive detection information; the interaction management module is used for managing information data in the system and completing information interaction between the system and the outside; by integrating a biosensing technology, intelligent data analysis and dynamic health management, high-sensitivity detection, accurate risk assessment and personalized health intervention of autoimmune diseases are realized, and the early diagnosis rate and the health management level are improved.

The invention provides a biomarker for early Alzheimer's disease detection, a kit, a preparation method and application. The kit mainly contains seven capture antibodies and detection antibodies which can be specifically combined with human blood metabolites, has the characteristics of high detection sensitivity, high specificity and high accuracy, not only can distinguish blood samples of forgetting mild cognitive impairment patients, but also can identify early-stage Alzheimer's disease patients.

The invention relates generally to variant activatable antibodies that include a masking moiety (MM), a cleavable moiety (CM), and an antibody (AB) that specifically binds to epidermal growth factor receptor (EGFR), and to methods of making and using these variant anti-EGFR activatable antibodies in a variety of therapeutic, diagnostic and prophylactic indications.

The invention provides a portunus trituberculatus SCP antigen, antibody, kit and application, and belongs to the technical field of allergen detection. The antigen of the portunus trituberculatus SCP comprises an antigen epitope peptide of the portunus trituberculatus SCP and a carrier protein, and the amino acid sequence of the antigen epitope peptide of the portunus trituberculatus SCP is as shown in SEQ ID NO. 14. The antigen epitope peptide of the antigen portunus trituberculatus SCP has a relatively stable structure before and after thermal processing, the detection limit of an antibody prepared from the antigen for detecting a crustacean allergen is 0.32 mu g / mL, the quantitation limit is 1.06 mu g / mL, the crustacean allergen can be specifically detected, and the antibody can be used for detecting the crustacean allergen. In addition, when the antibody is used for detecting the crustacean allergen, the accuracy is good, the matrix interference resistance and the precision are high, and the high detection effect on the crustacean allergen in food processed at high temperature can still be kept.

The invention discloses a recombinant CapA protein of cat astrovirus. The recombinant CapA protein has an amino acid sequence as shown in SEQ ID NO.1 in a sequence table or is coded by a base sequence as shown in SEQ ID NO.2 in the sequence table. Therefore, a cat astrovirus indirect ELISA antibody detection kit is developed and comprises a coated ELISA plate, and the coated ELISA plate takes the recombinant CapA protein as a coating antigen. Meanwhile, a corresponding indirect ELISA antibody detection method is established. Research results show that the critical value of the indirect ELISA antibody detection method and kit is 0.523; the sensitivity is 1: 32000; the in-batch variation coefficient of detected samples is 2.679%-7.910%, the inter-batch variation coefficient is 3.627%-8.982%, both the in-batch variation coefficient and the inter-batch variation coefficient are smaller than 10%, and the clinical application effect is good. Therefore, the kit has the characteristics of high sensitivity, strong specificity, good repeatability and the like, can quickly and effectively detect the FAstV antibody, and provides technical support for accurate diagnosis, timely prevention and control and vaccine effect evaluation of FAstV.

The invention relates to a feline calicivirus VP1 recombinant protein, a detection antibody, a detection test strip, a preparation method and application thereof. The amino acid sequence of the feline calicivirus VP1 recombinant protein is as shown in SEQ ID NO. 1. On the basis, the invention provides a monoclonal antibody for specifically recognizing the feline calicivirus and application of the recombinant protein in preparation of a kit for detecting the feline calicivirus antibody, in particular to a test strip for detecting the feline calicivirus antibody. According to the application, the industry blank that VP1 protein is used for antibody diagnosis is filled, the problem of missing detection caused by incomplete coverage of an antigen region is effectively solved, a brand new technical path is provided for improving the sensitivity and accuracy of FCV antibody diagnosis, and a core foundation is laid for research and development of subsequent high-cost-performance FCV diagnostic kits.

The invention relates to the technical field of allergy detection, in particular to an inhalation allergen specific IgE antibody detection kit and application thereof. The kit is a colloidal gold detection kit. The detection objects are pollen, dust mites and animal scurf; the kit comprises a colloidal gold labeled mouse anti-human IgE antibody arranged on a test strip combination pad; the pollen antigens (Art v1, Amb a1 and Bet v2), the dust mite antigens (Der f1 and Der p1) and the animal scurf antigen (Can f1) are arranged on a nitrocellulose membrane of the test strip. The kit provided by the invention can be used for rapidly identifying the allergen of the inhalation allergy symptom and classifying the severity of the allergy symptom, and the identification result has relatively high accuracy.

The invention discloses a detection method based on combination of anti-NF155 antibody detection and enzyme-linked immunosorbent assay. According to the detection method, an analysis model based on an XGBoost model algorithm is established by adopting anti-NF155 antibody detection of a patient and detection data of an enzyme-linked immunosorbent assay in combination with autologous clinical data of the patient, so that CIDP subtype classification is performed on the patient, and personalized treatment scheme suggestions are provided in combination with illness state dynamic data of the patient. Meanwhile, the invention provides a corresponding detection system which comprises a laboratory, a data storage library and an analysis unit, the anti-NF155 antibody detection is executed through laboratory equipment, and detection data and patient autologous clinical data are stored in the data storage library. The analysis unit establishes an analysis model based on an XGBoost model algorithm based on a machine learning algorithm.

The invention provides a nano photon structure chip, a preparation method thereof, a detection method and related equipment, and relates to the technical field of medical detection equipment, and the preparation method of the nano photon structure chip comprises the following steps: vertically dropwise adding a mixed solution containing PS nanoparticles and SDS onto a silicon wafer substrate; covering the area of the micro-wall template above the area where the mixed solution is dropwise added on the silicon wafer substrate, so that the PS nanoparticles are self-assembled to form a regular chain structure under the guidance of the micro-wall template and the SDS solution; dividing the regular chain structure into a plurality of detection areas, and fixing a marker detection antibody in each detection area to obtain the nano photon structure chip containing a plurality of marker detection antibody detection areas. The nano photon structure chip provided by the invention enhances an optical signal, and when the nano photon structure chip is applied to detection of biomarkers, low-concentration biomarkers in a biological sample can be quickly identified.