227 results about "Antibody detection" patented technology

The application belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody pair for detecting feline immunodeficiency virus p24 protein and application thereof. The monoclonal antibody pair is monoclonal antibody 1D8 and monoclonal antibody 1H10, which can specifically recognize feline immunodeficiency virus p24 protein, and the heavy chain and light chain variable region complementarity determining region sequences of 1D8 and 1H10 are clear, and are respectively shown as SEQ ID NO. 1-12. The antibody pair can effectively avoid cross reaction, and significantly improve the accuracy and sensitivity of detection; the colloidal gold detection test strip constructed based on the antibody pair is simple and fast in operation, is suitable for early antigen detection of FIV infection, solves the problem that existing antibody detection cannot distinguish natural infection and vaccine immunization, and provides a reliable technical means for on-site screening and disease prevention and control of FIV.

The invention relates to the technical fields of gene engineering, antibody engineering and immunodiagnosis. The invention provides a recombinant humanized anti-Cpn IgM monoclonal antibody. The monoclonal antibody comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises three complementary determining regions CDR1, CDR2 and CDR3, and the amino acid sequences of the three complementary determining regions are respectively shown as SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5; the heavy chain variable region comprises three complementary determining regions CDR1, CDR2 and CDR3, and the amino acid sequences of the three complementary determining regions are shown as SEQ ID NO.6, SEQ ID NO.7 and SEQ ID NO.8 respectively. The invention also provides a method for preparing the monoclonal antibody. The recombinant humanized anti-Cpn IgM monoclonal antibody provided by the invention is expressed in mammalian cells by utilizing a gene recombination technology, has the characteristics of high purity, small batch difference and the like, and can be applied to a Cpn IgM antibody detection kit as a quality control product; as a quality control product, the recombinant humanized anti-Cpn IgM monoclonal antibody has the advantages of high sensitivity, high safety, inter-batch stability and the like.

The invention discloses an indirect ELISA (enzyme-linked immuno sorbent assay) detection method of a porcine epidemic diarrhea virus antibody SIgA, and belongs to the technical field of animal antibody detection. In order to solve the problems of limited detection range and single immune evaluation dimension of a porcine epidemic diarrhea virus detection method in the prior art, the invention provides an indirect ELISA detection method of a porcine epidemic diarrhea virus antibody SIgA, and the detection method adopts a pichia pastoris eukaryotic secretion platform to prepare a high-activity PEDV S1 antigen; meanwhile, a secreting type IgA antibody is used as a detection target; the method has the advantages of high detection sensitivity, low background noise, better variant compatibility and practical popularization value, and provides a clinical and industrial-level solution for maternal immunity monitoring, piglet passive protection evaluation and vaccine immunity strategy optimization.

The invention relates to a feline calicivirus VP1 recombinant protein, a detection antibody, a detection test strip, a preparation method and application thereof. The amino acid sequence of the feline calicivirus VP1 recombinant protein is as shown in SEQ ID NO. 1. On the basis, the invention provides a monoclonal antibody for specifically recognizing the feline calicivirus and application of the recombinant protein in preparation of a kit for detecting the feline calicivirus antibody, in particular to a test strip for detecting the feline calicivirus antibody. According to the application, the industry blank that VP1 protein is used for antibody diagnosis is filled, the problem of missing detection caused by incomplete coverage of an antigen region is effectively solved, a brand new technical path is provided for improving the sensitivity and accuracy of FCV antibody diagnosis, and a core foundation is laid for research and development of subsequent high-cost-performance FCV diagnostic kits.

The application discloses a kit for detecting equine infectious anemia virus antibody and belongs to the field of animal virus antibody detection. The kit comprises equine infectious anemia virus p26-gp90 recombinant protein, mouse IgG and goat anti-mouse IgG, and the equine infectious anemia virus p26-gp90 recombinant protein is composed of an amino acid sequence shown in SEQ ID NO. 1. The kit is used for detecting equine infectious anemia virus antibody, is convenient and fast, has high sensitivity, has no cross reaction with other pathogens, has high specificity, has great clinical significance and wide application prospect.

The application relates to a detection method of anti-preS1 antibody, an anti-preS1 antibody detection kit and application, the application carries out multi-sequence comparison and conservation analysis on preS1 sequences of different genotypes (A, B, C, D and CD mixed type) of HBV, takes type A (AB697487.1), type C (FJ032351.1) and type D (LC519808.1) as reference sequences, based on the sequence consistency standard of 85% of full length and 90% of the key region of functional antibody binding, a highly conservative preS1 full-length antigen sequence is systematically screened out in natural variation, the sequence can cover antigen variation of A-D type HBV, and the sequence is taken as a coating antigen to establish an indirect ELISA detection system, a good linear relationship is presented in the range of 4.88-625 ng / mL, and the Cut-off value is 33.6 ng / mL; clinical verification shows that the method can effectively distinguish chronic hepatitis B patients from healthy control groups, compared with detection methods based on partial fragments or overlapping short fragments, the false negative rate can be significantly reduced, and the method is suitable for HBV infection immune state evaluation, anti-virus curative effect monitoring and prognosis analysis.

The invention discloses a real-time detection system and a monitoring and early warning method for African swine fever virus. The system comprises a latex microsphere labeled immunochromatography antibody detection card, a portable MINI-PCR instrument and a freeze-dried microsphere type fluorescent PCR detection reagent, the detection card takes the recombinant ASFV p30 protein as a target spot, antibody detection is completed within 10 minutes, the lowest detection limit is 1: 1024, and the specificity is greater than 99%; the PCR reagent adopts a double-primer double-probe design, and the lowest detection limit is 1 copy / microliter. According to the invention, through a collaborative process of antibody screening, nucleic acid detection and result evaluation, ASFV total infection cycle detection is realized, epidemic situation discovery time is advanced to 1-3 days after infection, and early warning response time is shortened by more than 60%; the system is simple to operate and low in cost, does not need professionals and laboratories, can be deployed in basic-level scenes such as pig farms and slaughter houses, effectively solves pain points of an existing detection system, and provides key technical support for prevention and control of African swine fever.

The invention relates to the technical field of detection kits, in particular to a milk specificity IgG antibody detection kit which comprises a detection kit body, a storage hole is formed in the bottom end of the detection kit body, the top end of the storage hole is communicated with a discharging cavity, and a feeding pipe fixedly penetrates through the middle of the side face of the detection kit body; the feeding pipe injects a sample solution into the discharging cavity, an extended cleaning mechanism is arranged on the inner wall of the storage hole, the top end of the discharging cavity is communicated with a detection cavity, and a cleaning mechanism, a sampling mechanism and a rotary detection mechanism are arranged on the inner wall of the detection cavity. The extended cleaning mechanism comprises a storage column arranged on the inner wall of the storage hole; the device can ensure that the cleaning scraping plate is continuously and tightly attached to the inner wall of the discharging cavity, the scraping efficiency is guaranteed, the cleaning quality is improved, the cleaning scraping plate can be stored and hidden after cleaning is completed, deformation caused by collision with a sampling rod during sampling is avoided, and the cleaning performance of the cleaning scraping plate is maintained.

This invention relates to the field of biotechnology, and in particular to an antigenic epitope peptide, product, and application for detecting antibodies produced by porcine deltacoronavirus infection. This invention identified short peptide epitopes in PDCoV longitudinal challenge serum samples, obtaining 12 short peptides, which were then prepared into a porcine deltacoronavirus antibody detection kit. Finally, six field serum samples were selected for antibody detection, and the effectiveness was verified using a virus neutralization experiment. The results showed that the porcine deltacoronavirus antibody detection kit provided by this invention is effective. Furthermore, the porcine deltacoronavirus antibody detection kit provided by this invention, as a novel detection method, improves sensitivity and specificity while simultaneously providing multiple detection results, namely, determining whether viral infection has occurred, identifying the stage of infection, and achieving DIVA (Differentiation, Infection, and Variability) analysis.

The application discloses a binding antibody against SARS-CoV-2 virus N protein and application thereof. The amino acid sequence of the heavy chain variable region of the monoclonal antibody N2E5 protected by the application is shown in SEQ ID NO. 1 in the sequence listing, and the amino acid sequence of the light chain variable region is shown in SEQ ID NO. 2 in the sequence listing; the amino acid sequence of the heavy chain variable region of the monoclonal antibody N2E5 is shown in SEQ ID NO. 5 in the sequence listing, and the amino acid sequence of the light chain variable region is shown in SEQ ID NO. 6 in the sequence listing. The kinetic constants K D of N2E5 and N8C6 with the N protein are 1.42*10 ‑8 M and 1.31*10 ‑8 M respectively. The antibody has high affinity with the N protein of the novel coronavirus and can be widely used in a novel coronavirus antibody detection kit and clinical treatment.

The utility model relates to the technical field of kits, and discloses an antibody detection kit. The protection assembly comprises a partition plate fixedly arranged in the box body, placement pads fixedly arranged in the box body and located on the left side and the right side of the partition plate, a top plate located above the partition plate, a pressing pad fixedly arranged below the top plate, a bandage located above the placement pads and hook-and-loop fasteners A fixedly arranged at the front end and the rear end of the bandage. The hook-and-loop fasteners B are fixedly arranged on the front side wall and the rear side wall in the box body, a plurality of containing grooves penetrating through the top wall are formed in the side wall, close to the partition plate, of the containing pad, the pressing pad is located over the containing pad, and the top plate is fixedly arranged between the partition plate and the box cover through buckles. The kit has the beneficial effect of preventing and protecting the reagent tube, has high protection performance, and solves the problem that the reagent tube collides with the inner wall of the kit when the kit is bumped in the transportation process; when the kit is violently collided, the antibody is damaged, and the subsequent use is influenced.

The application provides a quantum dot microsphere fluorescent immunochromatography test paper for combined detection of ASFV antibodies and nucleic acids and an application thereof, and belongs to the technical field of veterinary biological diagnosis products. The quantum dot microsphere fluorescent immunochromatography test paper for combined detection of ASFV antibodies and nucleic acids comprises a base plate, on the base plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are sequentially connected in a head-to-tail mode, from the combination pad to the water absorption pad, the nitrocellulose membrane is sequentially provided with an antibody detection line, a nucleic acid detection line and a quality control line, the antibody detection line is coated with a goat anti-pig IgG-Fc antibody, the nucleic acid detection line is coated with streptavidin, and the quality control line is coated with a rabbit anti-sheep IgG antibody; the combination pad is coated with a quantum dot microsphere labeled recombinant protein P22 and a quantum dot microsphere labeled sheep anti-digoxin antibody. The test paper can rapidly, highly specifically and highly sensitively detect ASFV antibodies and nucleic acids.

The present disclosure provides antibodies and polypeptides that specifically bind to nicotinic acid phosphoribosyltransferase (NAPRT). Also provided are nucleic acids encoding the antibodies, expression vectors and host cells for making the antibodies, methods of using the antibodies to detect NAPRT in a sample from a subject in need thereof, methods of using the antibodies to diagnose a subject having cancer or sepsis. Compositions comprising these antibodies are also provided. Also provided are compositions comprising a nicotinamide phosphoribosyltransferase inhibitor (NAMPTi), and methods of using the NAMPTi to treat cancer following diagnosis with the NAPRT antibody.

The utility model relates to the technical field of detection kits, in particular to a virus antibody detection kit which comprises a virus antibody detection kit body, an edge groove is formed in one end of the virus antibody detection kit body, a reagent dropping area is arranged on the top surface of the edge groove, and a medical silica gel cover is fixedly sleeved on the surface of the edge groove. The medical silica gel cover covers the reagent dropping area, a notch is formed in the surface of the virus antibody detection kit body, a transparent sealing sheet is fixed on the surface of the notch, and a marking paper strip is inserted between the notch and the transparent sealing sheet; the virus antibody detection kit provided by the utility model has the beneficial effects that the medical silica gel cover is arranged at the end part of the kit body, and the silica gel patch matched with the notch of the reagent dropping area is arranged at the bottom of the silica gel cover, so that the reagent dropping area is effectively protected in an unused state, external pollution and damage are avoided, and the service life of the reagent dropping area is prolonged. Therefore, the accuracy of the detection result is improved.

The invention belongs to the technical field of immunodetection, and discloses a VB12 sandwich method antibody mAb10 or an antigen binding fragment thereof, and a preparation method and application thereof. The VB12 sandwich method antibody mAb10 or the antigen binding fragment thereof provided by the invention comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises complementary determining regions LCDR1, LCDR2 and LCDR3, and the heavy chain variable region comprises complementary determining regions HCDR1, HCDR2 and HCDR3. When the VB12 sandwich method antibody mAb10 is used for detecting a VB12 standard antigen, the detection sensitivity is lower than 0.02 ng / mL, a clinical sample is detected through a magnetochemiluminescence method, the correlation with R clinical comparison is good in the sample range of 0-2 ng / mL, and the VB12 sandwich method antibody mAb10 has important significance in the field of diagnosis and treatment of VB12 deficiency related diseases.

The utility model discloses a flow antibody detection device, and relates to the technical field of detection devices.The flow antibody detection device comprises an operation panel, the operation panel is provided with a mixing assembly used for mixing an antibody and a fluorescent agent, the mixing assembly comprises a first motor installed on the upper surface of the operation panel, the output end of the first motor is fixedly connected with a rotating disc, and the rotating disc is provided with a clamping assembly; through cooperative arrangement of a rotating rod, a threaded rod, a sealing plate and other structures, during use, a moving block slides on the rotating rod, a moving ring sleeves a fixed rod through a sliding hole, then the rotating rod is rotated to enable the moving block to be in threaded connection with the threaded rod, and then the moving block is driven to descend; and the sealing plate is pressed above the vessel to seal the vessel, so that the problem of resource waste caused by easy spilling when the vessel is filled with too much water is avoided as much as possible.

This invention relates to the field of biopharmaceutical technology, and more particularly to a type of beeswax ( Fraxinus pennsylvanica This invention relates to a pollen allergen composition, its preparation method, and its application. It reveals that Chinese ash pollen contains more different allergenic proteins compared to European and American ash pollen. Therefore, using Chinese ash pollen as raw material, the resulting ash pollen allergen composition contains novel allergenic protein components that bind more strongly to the serum of Chinese ash pollen allergy patients, resulting in superior efficacy. This invention utilizes the ash pollen allergen composition to prepare a kit. This kit is highly sensitive, highly specific, and easy to operate. By changing the enzyme conjugate, it can be used for two purposes in one kit, achieving dual functions: detection of ash pollen allergen-specific IgE antibodies (in vitro allergen diagnosis) and quantitative detection of IgG antibodies (evaluation of desensitization therapy efficacy) in allergy patients.

The invention relates to the technical field of immunodetection, in particular to an anti-TPO antibody or an antigen binding fragment thereof. The anti-TPO antibody provided by the invention has the characteristics of high affinity and good stability, and provides a raw material source with excellent performance for the preparation of a calibration product / a positive control product / a quality control product of a TPO antibody detection kit.

The invention belongs to the technical field of biology, and particularly relates to a Prussian blue nano test strip for detecting an African horse pestivirus antibody as well as a preparation method and application of the Prussian blue nano test strip. A sample pad, a combination pad, a chromatography detection membrane and a water absorption pad are sequentially fixed on the supporting bottom plate; the chromatography detection membrane is provided with a detection line and a quality control line, the detection line is coated with staphylococcus A protein, and the quality control line is coated with a monoclonal antibody of African horse pestivirus VP7 recombinant protein; and the combination pad is coated with an African horse pestivirus VP7 delta N1-129 recombinant protein fragment coupled with Prussian blue nanoparticles. According to the invention, Prussian blue nano is used as a coupling tracer, and the specific reaction of an antigen and an antibody is fully utilized to detect the African horse pestivirus antibody. After the conjugate is combined with a corresponding antibody, a result can be clearly observed, and the conjugate is good in stability, high in sensitivity and strong in specificity, has a clinical application function and huge development potential, and is an extremely ideal antibody test strip detection material.

This invention discloses a kit for detecting porcine reproductive and respiratory syndrome virus (PRRSV) antibodies and its applications, belonging to the field of animal disease detection technology. The kit uses a mutant nucleocapsid protein (N protein) of the PRRSV-2 class NADC30 strain CHsx1401 as the coating antigen; the N protein mutant has mutations at amino acid positions 23, 75, and / or 90 compared to the wild-type N protein. High purity is achieved through optimized prokaryotic expression systems and purification processes. Under optimal detection conditions, the kit exhibits strong specificity, high sensitivity, and good reproducibility, with significantly superior detection performance compared to existing commercial kits. It can be used for PRRSV-2 antibody detection, epidemiological surveys, and disease control.

The invention discloses a platelet antibody detection kit and application thereof. Belongs to the technical field of detection kits. The kit comprises a microcolumn gel card, an enzyme-labeled secondary antibody, an enzyme-labeled secondary antibody diluent, a 20x washing solution, a developing solution, a fresh platelet treatment solution, a platelet buffer solution, a platelet preparation solution, a freeze-dried platelet reconstitution fluid, freeze-dried platelets and a negative control. The platelet antibody detection kit disclosed by the invention has the beneficial effects that the platelet antibody detection kit disclosed by the invention enables cross-matching of platelets to be possible; according to the invention, a micro-column gel immunoassay technology is combined with an enzyme immunolabeling technology, so that the limitation that only agglutination reaction can be carried out in a micro-column gel method is solved; according to the reaction card washing equipment disclosed by the invention, semi-automatic operation of micro-column gel method detection is realized; in the plasma, triglyceride is less than or equal to 5mg / mL, bilirubin is less than or equal to 20mg / dL, and hemoglobin is less than or equal to 5mg / mL, so that the result is not influenced; the kit has the advantages of good stability, long effective period, high sensitivity, strong specificity and simple operation.

The invention belongs to the technical field of detection, and particularly relates to an anti-swine IgA monoclonal antibody and application thereof in PEDV IgA antibody detection. The invention provides an anti-swine IgA monoclonal antibody and a sequence thereof, the monoclonal antibody is effective to swine IgA, but is ineffective to swine immune globulins IgG, IgM, IgD and IgE, and has good reactivity and specificity. According to the invention, an anti-swine IgA monoclonal antibody is labeled on lanthanide EU3 + fluorescent microspheres, and a time-resolved immunofluorescence test strip detection method is established and is used for qualitative detection of an IgA antibody. The detection method is wide in detection range, high in sensitivity and rapid in detection; good repeatability, accuracy and stability are achieved; in addition, the monoclonal antibody has no cross reaction with porcine immune globulins IgG, IgM, IgD and IgE, is high in specificity, has a clinical detection coincidence rate higher than 97.3%, and has strong clinical application potential.

The invention discloses an F1 monoclonal antibody and application thereof, the F1 monoclonal antibody is used for specifically binding a 6HB structural domain in a spike protein S2 subunit of SARS-CoV-2, the F1 monoclonal antibody comprises VHCDR1-3 of a heavy chain variable region and VLCDR1-3 of a light chain variable region, the amino acid sequences of the VHCDR1, the VHCDR2 and the VHCDR3 are respectively shown as SEQ ID NO.1, SEQ ID NO.3 and SEQ ID NO.5, and the amino acid sequences of the VLCDR1, the VLCDR2 and the VLCDR3 are respectively shown as SEQ ID NO.2, SEQ ID NO.4 and SEQ ID NO.6. The invention further discloses an application of the F1 monoclonal antibody. The F1 monoclonal antibody can neutralize SARS-CoV-2 virus infection, and has important application value in serological detection of new coronavirus, preparation of a reagent for new coronavirus infection detection and a reagent for new coronavirus antigen or antibody detection.

The invention provides an antibody detection kit for identifying porcine circovirus type 2 wild virus infection. The detection kit disclosed by the invention comprises a magnetic particle coupled PCV2Rep protein, a chemiluminescence labeled antibody and a chemiluminescence substrate solution. The detection kit disclosed by the invention takes PCV2Rep protein as an antigen, can rapidly and sensitively detect antibodies related to wild virus infection, can distinguish wild virus infection from vaccine immune response, and makes up the limitation of existing Cap protein antibody detection.

The utility model discloses an autoimmune liver disease antibody detection chip, which belongs to the technical field of biochips and diagnostic reagents and comprises a reaction membrane, an upper shell and a lower shell, the upper shell and the lower shell can be buckled together, a water absorption layer is arranged between the upper shell and the lower shell, a blood filtering component is movably arranged above the upper shell, and a water outlet is formed in the lower shell. The blood filtering assembly comprises a blood filtering cup and a blood filtering film, and the blood filtering film is fixedly connected with the blood filtering cup. According to the autoimmune liver disease antibody detection chip disclosed by the utility model, the blood filtering film can be flexibly mounted and dismounted, and the waste of the blood filtering film is avoided.

The application discloses an ELISA antibody detection kit for identifying Brucella gene deletion live vaccine (M5-90△26 strain) and wild strains and application. The kit comprises enzyme-linked reaction plate 1 and enzyme-linked reaction plate 2. The enzyme-linked reaction plate 1 is coated with LPS extracted from the Brucella gene deletion live vaccine (M5-90△26 strain), and the enzyme-linked reaction plate 2 is coated with a Brucella BP26 antigen epitope polypeptide combination composed of polypeptides with amino acid sequences shown in SEQ ID NO:1-2. The application is suitable for goats and sheep inoculated with the Brucella gene deletion live vaccine (M5-90△26 strain), can effectively distinguish the antibody produced by the Brucella gene deletion live vaccine (M5-90△26 strain) and the antibody produced by wild strain infection, thereby realizing identification and detection, being favorable for prevention and control of brucellosis and optimization of a vaccination strategy, and providing technical support for healthy and sustainable development of animal husbandry.

The invention belongs to the technical field of immunodetection, and discloses a folic acid sandwich method antibody mAb15 or an antigen binding fragment thereof as well as a preparation method and application of the folic acid sandwich method antibody mAb15 or the antigen binding fragment thereof. The folic acid sandwich antibody mAb15 or the antigen binding fragment thereof comprises a light chain variable region VL and a heavy chain variable region VH, the light chain variable region VL comprises complementary determining regions LCDR1, LCDR2 and LCDR3, and the heavy chain variable region VH comprises complementary determining regions HCDR1, HCDR2 and HCDR3. When the folic acid sandwich method antibody mAb15 is used for detecting a folic acid standard antigen, the detection sensitivity is lower than 0.15 ng / mL, a clinical sample is detected through a magnetochemiluminescence method, the correlation with R clinical comparison is good in the sample range of 0-20 ng / mL, and the folic acid sandwich method antibody mAb15 has important significance in the field of diagnosis and treatment of birth defects, cardiovascular diseases, tumors and neurodegenerative diseases.

The application discloses a reagent containing pig pseudorabies virus gB protein magnetic microparticle coating, a preparation method and a kit, and relates to the technical field of antibody detection. The preparation method of the reagent containing pig pseudorabies virus gB protein magnetic microparticle coating comprises the following steps: incubating magnetic microparticles and gB protein antibodies in a coating buffer solution; removing the reaction solution, and performing first sealing on the gB protein magnetic microparticle coating with a first sealing solution; after the first sealing is completed, the first sealing solution is washed away, and then the gB protein magnetic microparticle coating is sealed for the second time with a second sealing solution; after the second sealing is completed, the second sealing solution is washed away, and then resuspension is performed to obtain the reagent containing the gB protein magnetic microparticle coating. The two-step sealing method is adopted to prepare the reagent containing the pig pseudorabies virus gB protein magnetic microparticle coating, the sealing effect is good, and non-specific adsorption in the system is reduced. The kit provided by the application contains the reagent, and has the advantages of high analysis sensitivity, a wide linear range and high accuracy.

The invention discloses an ELISA (Enzyme-Linked Immunosorbent Assay) detection method for quantifying the content of an NKp46 antibody in an NK (Natural Killer) cell supernatant in vitro, and belongs to the technical field of antibody detection. The detection method comprises the following steps: capturing an NKp46 antibody in an NK cell supernatant through recombinant human NKp46 / NCR1 protein, carrying out combination by using an HRP-labeled goat anti-mouse IgG-Fc secondary antibody as an enzyme-labeled secondary antibody, carrying out catalytic color development by using a color development solution, fixing a color development result by using a stop solution, synchronously detecting an NKp46 antibody standard substance with a known concentration, drawing an OD450nm value-concentration standard curve, and quantifying the concentration of the NKp46 antibody according to the OD450nm value. The result shows that the content of the NKp46 antibody in the NK cell supernatant can be specifically, accurately and quantitatively detected by the detection method provided by the invention.