Sandwich method for detecting double antigen by antibody indirectly marked with nanometer granule and kit thereof

Abstract

The invention provides an antibody detection double-antigen sandwich method using nanoparticles. A nanoparticle label and a label between the labeled antigens implement an indirect labeling by combining the label on an antigen and a ligand which is labeled on the nanoparticle label and can specifically recognize the label, wherein the labeled antigen is a gene engineering recombined antigen. Withthe indirect labeling manner, the sensitivity and the specificity of the method can be remarkably improved.

Description

Technical field [0001] The invention relates to the field of immunodetection, in particular to an antibody detection double antigen sandwich method for indirect labeling of nano particles, and an immunoassay kit prepared by the method. Background technique [0002] For the detection of the target antibody in the specimen, immunological methods can be used for detection. Several methods are now widely used: radioimmunoassay, immunofluorescence, ELISA, etc., but they all have different degrees of shortcomings and deficiencies. [0003] Radioimmunoassay is subject to radioactive contamination, the detection time is long, and it requires professional equipment and professionals to perform single-person detection; the immunofluorescence method takes a long time to detect, requires professional equipment and professional operations, and is easily interfered by interfering substances. Positive; Enzyme-linked immunosorbent test (ELISA) has the advantages of high sensitivity and good spec...

AI Technical Summary

Problems solved by technology

[0009] 3. Since the labeled antigen generally contains a certain amount of miscellaneous proteins, the miscellaneous proteins are also labeled on the marker together with the target protein during the labeling process, resulting in a decrease in the specificity of the labeling complex;

[0010] 4. Conditions such as buffer solution, pH, and ionic strength for direct labeling cannot guarantee the optimal activity conditions of the labeled antigen. Factors such as vigorous stirring, centrifugation, and blocking during labeling are also likely to affect the active epitope of the labeled antigen, resulting in Reduced sensitivity of final detection

[0013] In summary, the markers used in the double-antigen sandwich detection kit for antibody detection based on recombinant antigen nanoparticles are currently using direct labeling, which has methodological defects. Therefore, further research is urgently needed on this basis. Improved, increased kit detection sensitivity and improved specificity

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