3 results about "Peptide substrate" patented technology

The application discloses a fluorescence hybridization chain reaction biosensor based on the existence of granzyme B and application thereof, and realizes real-time detection of T cell effector molecules by establishing a fluorescence hybridization chain reaction biosensor based on the existence of active granzyme B, and has the characteristics of short amplification time, simple operation, high amplification efficiency, simple reaction system and high economic benefit. By introducing peptide nucleic acid into the hybridization chain reaction system, the cutting of the substrate by granzyme B is successfully used as the starting recognition event of the hybridization chain reaction, and after the cutting of the peptide substrate in the peptide nucleic acid by granzyme B, the foothold is exposed, the strand displacement reaction is mediated, the fluorescence signal is restored after amplification, and a new strategy of using the hybridization chain reaction for detecting active cytokines is provided. The strategy makes the hybridization chain reaction using fluorescence as a signal output mode applicable to active protein detection, and has the advantages of a simple reaction system, few operation steps and low detection cost.

Disclosed herein are compositions and methods for the generation of peptide macrocycles from peptide substrates for ATP-grasp enzymes. The peptide substrates contain sufficient linkage cites to produce monocyclic, bicyclic, tricyclic, or multicyclic peptides comprising macrocyclic linkages selected from ω-ester linkages, ω-amide linkages, ω-thioester linkages, and combinations thereof.

This invention discloses a highly active transglutaminase polypeptide substrate and a screening method, belonging to the field of polypeptide screening technology. The screening method is based on molecular docking to obtain five pairs of highly active transglutaminase polypeptide substrates. After enzyme activity determination of the five pairs of polypeptide substrates, the pair with the highest activity was finally determined. The polypeptide substrate includes an acyl donor and an acyl acceptor. The acyl donor sequence is AFQSAY; the acyl acceptor sequence is FMKHKFV. The highly active mTGase polypeptide substrate provided in this application exhibits a 100-fold increased sensitivity to mTG compared to collagen. Using it to test enzyme activity can avoid substrate overabundance caused by non-specific coupling in biological samples, improve the efficiency of enzymatic reactions, and achieve highly sensitive detection of mTGase in the field of food safety.