Matrix metalloproteinase-2 specific multi-modality molecular image probe and preparation method and application in preparation of tumor imaging agent thereof

Abstract

The invention discloses a matrix metalloproteinase-2 specific multi-modality molecular image probe preparation method and application thereof. The preparation method comprises the following steps: 1)preparing peptide substrates for matrix metalloproteinase-2(MMP-2) specific identification; 2) modifying near infrared fluorescent dye on peptide substrate; 3) modifying fluorescent quenching group onpeptide substrate; 4) connecting different molecular weight PEG or tumor targeting group RGD on peptide substrate modified with near infrared fluorescent dye and fluorescent quenching group; 5) labeling the nuclide on the above modified peptide substrate side chain tyrosine. Compared with the prior art, the matrix metalloproteinase-2 specific multi-modality molecular image probe preparation method has the advantages that: 1) by utilizing the specificity and responsiveness of the MMP-2 protease by the probe, so that the probe is selectively enriched at the tumor site so as to improve the targeting property of the probe to the tumor; 2) performing multi-modality imaging in a living body tumor by using advanced molecular imaging technology such as optical, opto-acoustic, SPECT and the like,improving the sensitivity and accuracy of tumor imaging, and finally achieving accurate positioning of the tumor; 3) providing a new train of thought and method for early diagnosis, process research and prognosis evaluation of tumor.

Description

technical field

[0001] The invention belongs to the technical field of small-molecule fluorescent probes, and in particular relates to a method for preparing a matrix metalloproteinase-2-specific multimodal molecular imaging probe, a small-molecule probe prepared by the method, and such a small-molecule probe Application in imaging agents for early diagnosis of tumors. Background technique

[0002] Matrix metalloproteinase-2 (MMP-2) is a class of proteolytic enzymes that can degrade extracellular matrix, and plays a very important role in the growth, invasion, metastasis and angiogenesis of malignant tumors. Clinically, MMP-2 has become a reliable and valuable tumor biomarker, widely used in the early diagnosis and identification of tumors. The construction of MMP-2 protease-responsive molecular probes can not only improve the accuracy and sensitivity of tumor detection, but also use the properties of materials to treat tumors, which provides new ideas and methods for early...

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