70 results about "Active enzyme" patented technology

The invention discloses an ecological planting method for remarkably improving the amino acid content of citrus, which comprises the following steps: (1) mixing and fermenting pruned citrus branches, weed straws, livestock and poultry manure and indigenous probiotic ferment powder to prepare a basic microbial fertilizer, and adding a calcium magnesium phosphate fertilizer and monopotassium phosphate to prepare a special microbial fertilizer for citrus, applying as a base fertilizer in winter; (2) after citrus pruning, in key phenological periods such as a flower bud period, a young fruit period and a fruit swelling period, an indigenous probiotic enzyme diluent with a specific dilution ratio is used for systematic foliage spraying on a tree body. The indigenous probiotic enzyme contains bacillus amyloliquefaciens, saccharomycetes, active enzymes and a plurality of nutrient elements. The method can significantly increase the total amino acid content of citrus fruits, greatly increase the yield, improve the taste of the fruits, enhance the tree body resistance, reduce the use of chemical pesticides, improve soil, and realize quality improvement, yield increase and green sustainable development of citrus planting.

The invention discloses an efficient extraction method of large-molecular-weight astragalus polysaccharide, which belongs to the technical field of plant extraction, and comprises the following steps: cleaning astragalus, filtering, and crushing; the preparation method comprises the following steps: mixing a beta-1, 3-glucanase aqueous solution and trehalose ethylene diamine, and stirring for 10-30 minutes to obtain an enzyme protection system; soaking the radix astragali powder in a citric acid solution, adding an enzyme protection system, applying a bipolar pulsed electric field, and reacting at 40-60 DEG C for 30-40 minutes; adjusting the concentration of the ethanol to 40-50%, standing, centrifuging and collecting crude astragalus polysaccharide; purifying, decolorizing, concentrating, freeze-drying and crushing the crude astragalus polysaccharide to obtain refined astragalus polysaccharide; the mass ratio of the trehalose ethylene diamine to the beta-1, 3-glucanase is (15: 1) to (30: 1). The enzyme protection system maintains the activity of the enzyme, the enzyme and the pulsed electric field act synergistically, the extraction time is shortened, and the extraction rate is increased while the astragalus polysaccharide with large molecular weight is obtained.

The invention belongs to the technical field of bioengineering and microbiomics, and particularly relates to an acquisition method and application of a specific metabolic substrate of lactobacillus crispatus and application of a specific carbohydrate active enzyme CAZzyme gene and a polysaccharide utilization site (PUL). Genomes of lactobacillus crispatus and lactobacillus inertus are obtained from a public database, gene annotation is carried out on the genomes of the lactobacillus crispatus and the lactobacillus inertus, and a random forest model and a KEGG metabolic pathway diagram are combined to find specific CAZzyme genes, PUL and other genes with metabolic functions of the lactobacillus crispatus; a substrate which only lactobacillus crispatus can metabolize is searched based on the genome difference of the two bacteria, and the ability of the obtained substrate for promoting the growth of lactobacillus crispatus and inhibiting the growth of inert lactobacillus is evaluated through experiments. Growth and reproduction of the lactobacillus crispatus are promoted through the substrates, so that the lactobacillus crispatus accounts for a higher proportion in vagina microecology, the vagina is protected, and reproduction of other unfavorable bacteria is reduced. The assisted reproduction success rate is improved.

The invention discloses nutrient liquid for supplementing energy to human cells and a preparation method of the nutrient liquid, and belongs to the field of functional foods and beverages. The preparation method comprises the following steps: equivalently mixing fructus hippophae, longan pulp, Chinese dates, Chinese wolfberry fruits, mulberries, malt, raspberries and honey, sealing and fermenting, and filtering to obtain an active enzyme a stock solution; mixing Chinese yam, hawthorn, wheat, cinnamon, mulberry leaf, emblic leafflower fruit, kelp, momordica grosvenori, honeysuckle, kudzuvine root, rhizoma polygonati, black sesame, houttuynia cordata, purslane, dandelion, lucid ganoderma, ginseng, American ginseng, dogwood, eucommia ulmoides leaf and cistanche according to an equal amount to obtain a fermentation base material, mixing the fermentation base material with the active enzyme a stock solution, performing anaerobic fermentation, and performing low-temperature drying to obtain enzyme b; the stock solutions of the enzyme b and the active enzyme a are mixed, cooked and filtered to obtain mixed juice, a unique formula raw material is developed, and the nutrient solution with the good dissolved oxygen content is obtained through the sealed fermentation technology and the anaerobic fermentation technology.

The invention relates to the technical field of nutrient synergistic chelation and slow release, in particular to a preparation process of an organic water-soluble fertilizer containing polymeric amino acid, which comprises the following steps: preparing the polymeric amino acid, modified potassium fulvate, nanoscale microelement chelate and other raw materials in percentage by weight, and further comprises a bioactive enzyme compound; the preparation method comprises the following steps: modifying potassium fulvate, and adding nano titanium dioxide and carbon nanotubes; preparing a chelate, and adding a novel surfactant; when the compound is synthesized, biological active enzyme is added; finally, mixing and preparing, sequentially adding the materials, stirring, adding the preservative, and filtering to obtain the water-soluble fertilizer, and each step has clear temperature, time and operation requirements. The water-soluble fertilizer is scientific in formula, polymeric amino acid and the like have a synergistic effect, and the utilization rate of the fertilizer is increased; the water-retaining agent is good in water retention, and the biological active enzyme improves the soil; the preparation process is stable and environment-friendly, can improve crop yield and quality, reduces pollution, and brings economic and ecological double benefits.

This application discloses a method, a biosensor, and a related device for detecting the concentration of a target analyte. The biosensor includes a first working electrode and a second working electrode. When the tip of the biosensor is implanted subcutaneously with a hard needle, the second working electrode is closer to the needle tip than the first working electrode. An active enzyme is disposed on the first working electrode. After implantation, the first working electrode generates a first current, which includes the current generated by the reaction between the target analyte and the active enzyme, and the current generated by the reaction between an interfering substance and the first working electrode. An inactivating enzyme is disposed on the second working electrode. After implantation, the second working electrode generates a second current, which includes the current generated by the reaction between the interfering substance and the second working electrode. Thus, by using the first and second currents, the influence of interfering substances can be eliminated, improving the accuracy of the target analyte concentration calculation.

This invention discloses a highly efficient extraction method for plant cell vesicles based on the synergistic effect of three-dimensional gradient microvortex and acoustic fluidization, and its application. The plant cell vesicle extraction method provided by this invention achieves high yield, high purity, and high activity of plant cell vesicles through a full-chain process of active enzymatic hydrolysis, acoustic lysis, and three-dimensional gradient separation, enabling large-scale production. This method overcomes the shortcomings of traditional extraction methods, such as low yield and purity, chemical contamination, low extraction efficiency, uncontrollable vesicle size and easily damaged structure, and long extraction time. It allows for the large-scale production of more cosmetics and pharmaceuticals containing plant cell vesicles. This invention systematically solves the global challenge of plant vesicle extraction and application through multidisciplinary innovation (acoustics, fluid mechanics, and molecular biomimetic), achieving high extraction efficiency, good activity, and leading technical parameters, which is of great significance for the extraction and development of plant cell vesicles.

The invention discloses a tea tree pruning efficient degradation microbial combined bacterium, a microbial agent and application of the microbial agent, the tea tree pruning efficient degradation microbial combined bacterium comprises bacillus JKB11 and paenibacillus polymyxa JKB16, and the JKB11 and JKB16 combination in the tea tree pruning efficient degradation microbial combined bacterium can effectively deal with degradation of tea tree pruning; although the living enzymes of cellulose degrading enzyme (CMCase) and lignin degrading enzyme (lignin peroxidase (LiP) and manganese peroxidase (MnP)) are similar to those of JKB16, the three-element content and infrared spectrum indexes of the degradation product prove that the tea tree pruning degradation effect of the combination of JKB11and JKB16 is better.

The invention discloses a slow-digestion and high-satiety hydrolyzed polysaccharide-egg white protein compound and a preparation method thereof, and belongs to the technical field of modified protein. The preparation method comprises the following steps: dissolving polysaccharide in water, then adding active enzyme to carry out enzymolysis reaction, then carrying out enzyme deactivation, then adding egg white protein, and finally carrying out thermal crosslinking and drying to obtain the hydrolyzed polysaccharide-egg white protein compound. By regulating the hydrolysis degree of the polysaccharide and the glycosylation reaction degree of the polysaccharide and the protein, the digestion rate of the protein in the gastrointestinal tract can be effectively regulated, the sustained release of amino acid is realized, and the satiety maintenance time is prolonged. Meanwhile, the preparation method can also improve the structural characteristics and sensory quality of the compound and improve the acceptability of consumers.

The application relates to the technical field of metal recovery, in particular to a metal removal and recovery method for waste titanium anode plates; nanometer particles are subjected to a coating operation, cations are introduced to graft the coated nanometer particles, grafted nanometer particles are obtained, and the grafted nanometer particles are pretreated to obtain initial nanometer particles. In the application, the metal recovery of the waste titanium anode plates is realized through the synergistic effect of magnetic nanometer particle synthesis, functional modification and biological enzyme directional degradation; the surface of the nanometer particles is subjected to double-layer coating of silicon dioxide-polyacrylic acid and synergistic modification of carboxyl and amino groups, the adsorption capacity and selectivity to noble metal ions are enhanced, the noble metal ions are reduced to metal states through catalysis of an active enzyme solution, an integrated process of adsorption to degradation and then magnetic separation is formed between the noble metal ions and the functional nanometer particles, and after the titanium matrix is regenerated through pickling, high-value recovery and utilization of metal materials can be realized through smelting or powder metallurgy.

The application discloses a plant selenoprotein enzyme peptide composition, effervescent tablets and application thereof, and relates to the field of functional food, and comprises the following components in parts by weight: selenium-rich shepherd's purse powder 5-12 parts; comprehensive fruit and vegetable fermentation powder 8-15 parts; soybean peptide powder 3-10 parts; comprehensive digestive enzyme 05-3 parts; and pharmaceutically or dietetically acceptable excipients; the plant selenoprotein enzyme peptide composition, the effervescent tablets and the application thereof are scientifically combined with organic selenium (selenium-rich shepherd's purse powder), active enzymes and small-molecule metabolites generated by fermentation of various fruits and vegetables, soybean peptides easy to absorb and prebiotics. Selenium is the core of the antioxidant system, peptides and fermentation products provide nutritional substrates and regulatory signals, prebiotics improve the intestinal microenvironment, and multiple components synergistically act on multiple pathways such as immunity and metabolism.

The invention discloses an efficient composite immobilized enzyme for preparing ethyl R (-)-4-cyano-3-hydroxybutyrate and a preparation method of the efficient composite immobilized enzyme, and relates to the technical field of biological catalysis. The composite immobilized enzyme comprises an active enzyme component, a Nicoordination modified mesoporous silica-chitosan composite carrier, and a sodium alginate auxiliary embedding-glutaraldehyde covalent cross-linking composite immobilization mode. The preparation method comprises the following steps: preparing a modified carrier, preparing an active enzyme mixed solution, carrying out an embedding-crosslinking reaction, collecting and drying. The compound immobilized enzyme can catalytically prepare ethyl R (-)-4-cyano-3-hydroxybutyrate, the substrate conversion rate is still kept at an extremely high level after the compound immobilized enzyme is repeatedly used for more than 15 times, and the compound immobilized enzyme is stable in enzyme activity, low in cost and suitable for industrial green production of statin drug intermediates.

The invention discloses a flux cleaning agent for PCB assembly and a preparation method thereof, and relates to the technical field of flux cleaning agent preparation, and the preparation method comprises the following steps: adding xanthan gum into deionized water, adding an ion adsorbent and an antioxidant, and stirring and mixing uniformly to obtain a water phase; the preparation method comprises the following steps: adding a surfactant and oleic acid bis-imidazoline quaternary ammonium salt into an organic solvent, and uniformly stirring and mixing to obtain an organic phase; adding the organic phase into the water phase, uniformly stirring and mixing, adding the metal organic framework-active enzyme compound, uniformly stirring and mixing, clarifying and defoaming to obtain the flux cleaning agent. The flux cleaning agent prepared by the invention can clean residual flux in gaps and adsorb residual halogen elements, so that the corrosion risk is reduced.

The invention provides cranberry honey ice cream and a preparation method thereof, and the cranberry honey ice cream is prepared from the following raw materials based on 100 jin of finished products: 8 jin of powdered sugar, 2.5 jin of non-dairy creamer, 12 jin of honey, 65 g of a stabilizer, 15 g of a composite additive, 3 g of ethyl maltol, 3.5 g of vanillin, 15 g of molasses element, 80 jin of water, 50 g of essence, a proper amount of carmine, 3 jin of cranberry freeze-dried powder, 5 jin of natural cranberry juice and 2 g of sodium ascorbate. The stabilizer is formed by compounding xanthan gum and guar gum according to the mass ratio of 2: 1; the composite additive is prepared by compounding monoglyceride and polyglycerol fatty acid ester according to a mass ratio of 3: 1; the Changbai mountain linden honey, the cranberry freeze-dried powder and the natural cranberry juice are fused and matched with the high-purity sodium ascorbate, so that the nutritional structure is enriched, and functional components such as honey active enzyme and cranberry anthocyanin are effectively reserved; a compounding system of xanthan gum and guar gum and a compounding system of monoglyceride and polyglycerol fatty acid ester are adopted, so that the stability of a raw material system is remarkably improved.

The invention discloses a belt dryer for drying fruit and vegetable enzyme residues, the belt dryer comprises a mounting base, the top of the mounting base is fixedly provided with a drying box through bolts, the drying box is internally provided with a conveying mechanism, and the drying box is provided with a driving mechanism, a first drying mechanism, a second drying mechanism, a blowing mechanism and an exhaust mechanism; a first adsorption mechanism is arranged at the other end of the drying box, a second air suction mechanism is arranged at the bottom of an exhaust mechanism, and a dehumidification mechanism is arranged on the outer side of the mounting base. The first drying mechanism and the second drying mechanism are arranged, and a layered gradient temperature control structure is adopted, so that thermal degradation of enzyme active ingredients is avoided, the problem of local over-dryness or residual wet spots caused by single traditional drying temperature is solved, and the drying treatment uniformity of the fruit and vegetable enzyme residues is improved; and drying operation in multiple directions is adopted, a penetrating type air channel can be formed for the fruit and vegetable enzyme residues, the caking condition of the fruit and vegetable enzyme residues is avoided, and the drying operation efficiency is improved.

The invention discloses a method for extracting procyanidine from green kernels and black beans, and belongs to the technical field of plant active ingredient extraction. Mixing and dispersing the green kernel and black bean skin powder, the immobilized active enzyme and water to form a first mixed solution, performing ultrasonic treatment on the first mixed solution, and performing centrifugal separation to obtain a first extracting solution and filter residues; mixing and dispersing the filter residues and a mixed solvent to form a second mixed solution, performing hydrothermal treatment on the second mixed solution, and performing centrifugal separation to obtain a second extracting solution; mixing the first extracting solution and the second extracting solution, filtering to obtain filtrate, and purifying the filtrate through macroporous resin to obtain procyanidine-containing filtrate so as to finish extraction. By means of the preparation technology, safe and environment-friendly extraction of the procyanidine is achieved, and the extraction efficiency of the procyanidine is good.

The application provides a control method and device of a cooking appliance and the cooking appliance. The control method of the cooking appliance comprises the following steps: in response to a cooking instruction, the temperature in a cooking cavity is controlled to be kept in a first preset temperature range; when the temperature in the cooking cavity reaches the first preset temperature range, a UV light source is turned on and kept for a first preset time length; and the first preset temperature range is a range of active temperatures corresponding to active enzymes in food to be cooked.

The invention relates to the technical field of mesona chinensis jelly, and discloses a preparation process of mesona chinensis jelly, mesona chinensis jelly is prepared from mesona chinensis powder, and the mesona chinensis powder is prepared by the following steps: (1) raw material acquisition: picking mesona chinensis leaves, cleaning and disinfecting; (2) inactivating active enzyme: treating the mesona chinensis leaves obtained in the step (1) for 80-120s at the temperature of more than or equal to 90 DEG C to inactivate the active enzyme in the mesona chinensis leaves, and then quickly cooling to protect chlorophyll; (3) controlling the moisture content: freezing the mesona chinensis leaves obtained in the step (2), and then drying under a vacuum condition to remove the moisture of the mesona chinensis leaves; and (4) crushing: crushing the mesona chinensis leaves treated in the step (4), sieving with a 40-mesh sieve, and then carrying out ball-milling superfine crushing to obtain the green mesona chinensis powder. The green mesona chinensis powder is prepared by inhibiting polyphenol oxidase, protecting a chlorophyll structure and other dimensions, and the fresh green mesona chinensis jelly can be prepared by taking the green mesona chinensis powder as a raw material.

This invention discloses an intelligent fermentation device and method for processing plant active enzyme beverages, relating to the field of fermentation device technology. It includes a tank and a rotating stirring mechanism inside it. One end of the tank is equipped with a drive mechanism to rotate the stirring mechanism. The stirring mechanism inside the tank periodically stirs the fermenting material to promote fermentation. The device is designed as a drum, and the drive mechanism can drive the stirring mechanism to rotate independently or simultaneously drive the tank and stirring mechanism to rotate in opposite directions, completely removing sediment adhering to the bottom of the tank's inner wall, thus allowing for more complete fermentation. Furthermore, to prevent gas buildup inside the tank after fermentation, which could increase internal pressure and cause leakage of the fermentation liquid from the stirring mechanism's shaft, the shaft sleeve is pressurized. This utilizes greater external air pressure to reverse and confine the fermentation liquid inside the tank, effectively preventing contamination.

This invention provides a hydrophilic bifunctional probe and its synthesis and application. Specifically, this invention provides a hydrophilic bifunctional probe (6-(1-aminoethyl)naphthyl-2-yl)glycine (N1) and its corresponding fluorescent molecule (6-acetylnaphthyl-2-yl)glycine (N2). This pair of molecules in this invention exhibits high photostability and water solubility, and can be used in aqueous luminescent materials, biofluorescent probes, and biofluorescent imaging. It can be applied to fluorescence-based high-throughput screening systems to identify strains of highly active enzymes through quantitative detection in trace systems. The structures of the hydrophilic bifunctional probe and its corresponding fluorescent molecule are as follows: [structures omitted].

The invention discloses a stomach-protecting compound beverage taking elaeagnus angustifolia and honey as core functional raw materials and a preparation method thereof, and belongs to the technical field of foods. The elaeagnus angustifolia is a mature fruit of elaeagnus elaeagnus deciduous tree in the family of elaeagnus pungens, and is rich in polysaccharides, polyphenols and dietary fibers; the honey contains fructo-oligosaccharide and a plurality of active enzymes. The two components are compounded to achieve a synergistic effect, mildly assist in improving gastrointestinal functions, repairing mucous membranes and regulating flora balance. The beverage is prepared from the following components in percentage by weight: 25 to 60 percent of elaeagnus angustifolia fruit extracting solution, 0.25 to 6 percent of (honey and cane sugar), 0.0025 to 0.025 percent of sucralose, 0.05 to 0.3 percent of organic acid system (citric acid monohydrate, DL-tartaric acid and sodium citrate), 0.005 to 0.015 percent of vitamin C, 0.001 to 0.04 percent of iodine-free edible salt and the balance of purified water. The health-care beverage is prepared by scientific compatibility and a mild process, keeps fresh and natural taste, has multiple effects of promoting digestion, repairing gastrointestinal mucosa and regulating intestinal flora, and can be safely drunk for a long time.

This invention discloses a mesoporous silica nanoparticle, its preparation method, pharmaceutical composition, and applications. The nanoparticle is epoxy-modified, with its mesoporous channels loaded with polypeptide precursor molecules grafted onto chemotherapeutic drug molecules. Its outer layer is coated with an acid-sensitive polymer layer, enabling it to trigger drug release in the slightly acidic environment of the tumor. Furthermore, under the catalysis of highly expressed active enzymes within the nanoparticle, in-situ gelation occurs at the lesion site, thereby prolonging the retention time of the nanomedicine, improving the bioavailability of chemotherapeutic drugs, and enhancing the efficacy of chemotherapy.

The invention relates to a lipase mutant as well as a preparation method and application thereof, and the lipase mutant is obtained by performing single-site mutation or double-site mutation on 138-site arginine and 251-site tyrosine on the basis of lipase of which the nucleotide sequence is SEQ ID No.1 and the amino acid sequence is SEQ ID No.2. The lipase mutant or a recombinant expression vector or recombinant engineering bacteria thereof is used as an active enzyme, and a powerful biocatalyst is provided for synthesis of (S)-parachloro-beta-phenylalaninol. The invention provides a lipase mutant with remarkably improved catalytic activity, a nucleotide sequence of the lipase mutant, and a recombinant expression vector and recombinant engineering bacteria containing corresponding mutant genes, and the lipase mutant or recombinant cells containing corresponding mutant proteins are subjected to asymmetric reduction to obtain the lipase mutant. And the chiral (S)-p-chloro-beta-phenylalaninol with high optical purity can be prepared.

The invention relates to collagen hydrogel, a preparation method thereof and application of the collagen hydrogel in delivery of hangover-alleviating active enzyme. According to the collagen hydrogel provided by the invention, the collagen-based hydrogel is combined with the micro-scale grid and the artificial villus structure, so that the effects of prolonging the retention time in the stomach, enhancing the mechanical toughness and improving the compressive strength are achieved under physiological conditions, and the long-standing problem of balancing between the mechanical stability and the catalytic efficiency is solved; meanwhile, the excellent biocompatibility is realized. Taking hangover alleviating application as an example, the acetaldehyde removal efficiency of the system is improved by 30 times in a simulated stomach environment by fixing a double-enzyme cascade system of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH), and good quick hangover alleviating application potential is shown.

This application relates to the field of agricultural fertilizer technology and discloses a preparation process for a high-efficiency, low-temperature growth-promoting tetranitrogen liquid fertilizer, comprising the following steps: Step 1, selecting high-quality sheepskin as the organic nitrogen source and treating the sheepskin; Step 2, hydrolyzing the sheepskin at 55℃ using microorganisms and active enzymes for 24 hours; Step 3, performing preliminary filtration on the hydrolyzed mixture; Step 4, further purifying the amino acid liquid through multi-stage filtration; Step 5, concentrating the amino acid liquid to 40% concentration using vacuum evaporation technology; Step 6, mixing the concentrated amino acid liquid with urea ammonium nitrate solution, magnesium sulfate, ferrous sulfate, seaweed concentrate, nitrification inhibitor, and excipients in a specific ratio. By scientifically proportioning the high-concentration amino acid liquid with urea ammonium nitrate, magnesium sulfate, ferrous sulfate, seaweed concentrate, and nitrification inhibitor, comprehensive nutritional support is provided for crops, effectively improving crop growth rate, stress resistance, and yield.

The invention relates to the technical field of biology, in particular to a molecular marker related to the content of linarin in chrysanthemum indicum, a primer and application of the molecular marker. The molecular marker provided by the invention is located in an active enzyme which directly participates in the last step of a linarin synthesis step, and can more directly, accurately and quickly identify whether the linarin content in a chrysanthemum indicum variety is high or low. The invention also provides a method for detecting chrysanthemum indicum with high linarin content or chrysanthemum indicum with low linarin content by using the molecular marker, the operation is simple and easy, the result is stable and reliable, and a technical guarantee is provided for the protection of germplasm resources and new varieties of chrysanthemum indicum.

The application discloses a kind of cultivation methods of high SOD content sugarcane and bamboo cane intercropping, comprising the following steps: seedling treatment;Land preparation planting;Planting period management;According to mature condition, batch harvest bamboo cane and sugarcane.The application is by applying specific fertilizer multiple times in the growth cycle of sugarcane, and using the mode of sugarcane and bamboo cane intercropping, increase the absorption effect of fertilizer in soil in the root of sugarcane, promote the generation of high content SOD active enzyme in sugarcane.

The invention belongs to the field of flower preservation, and provides a fresh flower nutrition preservation SAA active enzyme + which is prepared from the following raw materials in parts by mass: 0.05-3.5 parts of enzyme, 0.01-2.2 parts of food coloring, 1-3 parts of vitamin C, 0.5-3 parts of ferrous sulfate, 1-10 parts of glucose, 1-5 parts of xylitol, 0.5-3 parts of trehalose, 1-5 parts of a pH regulator, 0.5-2 parts of a pearling agent, 0.1-2 parts of a touch regulator and 100 parts of water. The anti-gilding flower essence does not contain toxic components damaging plants, has a gilding sense, and can delay flower aging by proportioning the nutritional agent, the pH regulator, the bacteriostatic agent and the antioxidant according to a certain ratio.

The invention provides a daidzein reductase mutant as well as a construction method and application thereof, and belongs to the technical field of active enzymes. The invention provides a daidzein reductase mutant. The daidzein reductase mutant is obtained by mutation on the basis of wild daidzein reductase. Compared with a wild type DZNR, the daidzein reductase mutant disclosed by the invention has higher catalytic activity. Moreover, the mutant disclosed by the invention shows stronger tolerance in the presence of high-concentration equol, and the application of the daidzein reductase mutant can promote the preparation efficiency of equol and derivatives thereof.

This invention relates to the field of medicinal plant cultivation technology, specifically a cultivation method and application for significantly increasing the mannan content of Dendrobium officinale. The method includes: selecting a suitable cedar forest environment, fixing Dendrobium officinale seedlings using a soilless planting method, watering regularly, and spraying with a 200-fold diluted indigenous probiotic cultivation enzyme every 10 days, for a total of 10 sprays. The enzyme is fermented from native microorganisms in natural forest soil and plant materials, containing Bacillus amyloliquefaciens, yeast, active enzymes, and various minerals. This invention can significantly increase the yield of Dendrobium officinale and the content of active ingredients such as mannan and total amino acids, achieving green, efficient, and sustainable cultivation.