163 results about "Active enzyme" patented technology

The invention relates to the field of enzyme engineering, in particular to modified xylanase, a DNA (deoxyribonucleic acid) molecule, a recombinant plasmid and a transfected cell. The modified xylanase protected by the application is formed by replacing a deletion segment of a receptor enzyme with a substitution segment, the receptor enzyme is wild type xylanase, the sequence of the receptor enzyme is SEQ ID NO: 1, the deletion segment comprises a carbohydrate binding module of the receptor enzyme, the substitution segment is a carbohydrate binding module of a donor enzyme, the donor enzyme is a carbohydrate active enzyme, and the donor enzyme is a carbohydrate active enzyme. The sequence of the donor enzyme is one of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5. The carbohydrate binding module of the carbohydrate active enzyme with relatively high activity is replaced into the receptor enzyme, so that the binding capacity of the receptor enzyme and the carbohydrate is improved, and the activity of the receptor enzyme is improved.

The invention provides an additive composition containing a bioactive enzyme and a preparation method of the additive composition, and belongs to the technical field of biological enzymes. The preparation method comprises the following steps: fixing phytic acid and cyclodextrin on hydroxyapatite, modifying with tannic acid, reacting with dithiothreitol modified lysozyme to prepare an immobilized compound, adding 2, 2-dimethoxy-2-phenylacetophenone and methyl methacrylate, and carrying out irradiation reaction with an ultraviolet lamp to prepare the additive composition containing the bioactive enzyme. The broad-spectrum antibacterial property and the antiviral property of the lysozyme are greatly improved, the sterilization effect on gram negative bacteria is obviously improved, meanwhile, the stability of enzyme is greatly improved, the enzyme is not prone to inactivation, the preservation time of the enzyme is prolonged, the application effect of the enzyme is widened, meanwhile, the lysozyme has good killing capacity on oral bacteria and viruses, the addition of preservatives is reduced, and the cost is reduced. The cleaning effect and stability of the product are improved, tooth demineralization can be prevented, decayed teeth can be prevented, and wide application prospects are achieved.

The present application relates to the technical field of daily chemical cleaning products, and discloses a kind of wormwood leaf, enzyme laundry detergent, including the following components, by weight percentage: wormwood leaf enzyme: 30-50wt%, environmental protection enzyme: 10-20wt%, composite surfactant: 5-10wt%, including fatty alcohol polyoxyethylene ether sulfate and alkyl glycoside, wherein fatty alcohol polyoxyethylene ether sulfate accounts for 60-80wt% of the total amount of composite surfactant, and alkyl glycoside accounts for 20-40wt% of the total amount of composite surfactant; Dispersant: 0.3-1wt%; Enzyme-surfactant composite catalyst: 1-5wt%. The present invention adopts the technical scheme of combining wormwood leaf enzyme with environmental protection enzyme, extracts active enzyme and natural functional ingredients by fermentation, and realizes efficient decomposition of organic stains such as grease and protein. Compared with the scheme of using only chemical surfactant in the prior art, the problem of environmental pollution and skin irritation of chemical detergent is solved, and the safety and environmental protection of washing are significantly improved.

The invention discloses an ecological planting method for remarkably improving the amino acid content of citrus, which comprises the following steps: (1) mixing and fermenting pruned citrus branches, weed straws, livestock and poultry manure and indigenous probiotic ferment powder to prepare a basic microbial fertilizer, and adding a calcium magnesium phosphate fertilizer and monopotassium phosphate to prepare a special microbial fertilizer for citrus, applying as a base fertilizer in winter; (2) after citrus pruning, in key phenological periods such as a flower bud period, a young fruit period and a fruit swelling period, an indigenous probiotic enzyme diluent with a specific dilution ratio is used for systematic foliage spraying on a tree body. The indigenous probiotic enzyme contains bacillus amyloliquefaciens, saccharomycetes, active enzymes and a plurality of nutrient elements. The method can significantly increase the total amino acid content of citrus fruits, greatly increase the yield, improve the taste of the fruits, enhance the tree body resistance, reduce the use of chemical pesticides, improve soil, and realize quality improvement, yield increase and green sustainable development of citrus planting.

The invention provides a bean product fermentation influence factor analysis method and system, and relates to the technical field of food safety detection.The method comprises the steps that multiple sets of key parameters are set within a preset range, physical and chemical index changes under the parameters are monitored in real time, and the physical and chemical index changes comprise the tannin variation, the acid production capacity, the fermentation rate and the active enzyme variation; the method comprises the following steps: establishing a key parameter, constructing a physical and chemical index prediction model, training by taking the key parameter as input, evaluating a fermented bean product, training a sensory evaluation index prediction model by taking the physical and chemical index as input, and finally, determining an optimal combination of the key parameter through a genetic algorithm by taking maximization of the sensory evaluation index as a target, thereby optimizing a fermentation process. By setting and adjusting key fermentation parameters in real time and combining physical and chemical index monitoring and sensory evaluation, the purposes of optimizing the fermentation process and improving the final quality of bean products are achieved.

The invention relates to the technical field of data processing in the preparation process of probiotic high-activity enzyme freeze-dried powder, provides probiotic high-activity enzyme freeze-dried powder fermentation equipment and a fermentation process, and aims to solve the problem of inaccurate data caused by reduction of sensor precision in the fermentation process. Through integration of data acquisition, task generation, equipment execution, data processing, data classification, error range determination, sensor parameter adjustment and other modules, comprehensive monitoring of the fermentation process and intelligent adjustment of sensor configuration are realized, fermentation equipment control parameters are optimized by using a random forest model, a quality database is established to trace and analyze quality problems, and the intelligent control of the fermentation equipment is realized. The sensor configuration is intelligently adjusted according to historical data and real-time data, and the accuracy of sensor data acquisition is improved, so that the fermentation efficiency and the product quality stability are improved.

The invention discloses an efficient extraction method of large-molecular-weight astragalus polysaccharide, which belongs to the technical field of plant extraction, and comprises the following steps: cleaning astragalus, filtering, and crushing; the preparation method comprises the following steps: mixing a beta-1, 3-glucanase aqueous solution and trehalose ethylene diamine, and stirring for 10-30 minutes to obtain an enzyme protection system; soaking the radix astragali powder in a citric acid solution, adding an enzyme protection system, applying a bipolar pulsed electric field, and reacting at 40-60 DEG C for 30-40 minutes; adjusting the concentration of the ethanol to 40-50%, standing, centrifuging and collecting crude astragalus polysaccharide; purifying, decolorizing, concentrating, freeze-drying and crushing the crude astragalus polysaccharide to obtain refined astragalus polysaccharide; the mass ratio of the trehalose ethylene diamine to the beta-1, 3-glucanase is (15: 1) to (30: 1). The enzyme protection system maintains the activity of the enzyme, the enzyme and the pulsed electric field act synergistically, the extraction time is shortened, and the extraction rate is increased while the astragalus polysaccharide with large molecular weight is obtained.

A coupled enzyme reaction point of care assay system detects and measures an active enzyme biomarker from a patient's blood sample. The assay system has a point of care blood collector for collecting blood into a blood collection vial. A biomarker detection mechanism has at least three fluid flow entities. Each fluid flow entity has a fluid input zone and a corresponding reaction zone. The fluid flow entities include a test strip, a negative control strip and a positive control strip. The reaction zones include components from a multiplicity of substrates, co-factors, buffers and cryoprotectants, and a multiplicity of tethered enzymes. At least one of the tethered enzymes is an enzyme that produces luminescence. The tethered enzymes are adapted to react to a constituent which may be a biomarker. A photonic luminescence reader measures light emitted from an active enzyme biomarker present in the patient's blood.

The invention belongs to the technical field of artificial wetland regenerated water treatment, and particularly relates to an enhanced removal method for nitrate nitrogen and organic matters in an artificial wetland regenerated water source. According to the method, glucose, manganese ions and copper ions are added into a regenerated water source, heterotrophic bacteria are enriched in a constructed wetland packed bed, metal ions are loaded in the constructed wetland packed bed, extracellular active enzymes are generated through stress reaction of the heterotrophic bacteria to the metal ions, and then the enzymolysis process of humus organic matters difficult to biodegrade is achieved. The biodegradability of the recycled water is improved, and finally, the removal process of nitrate nitrogen and organic matters is enhanced.

The invention discloses a plant cell vesicle efficient extraction method based on the synergistic effect of three-dimensional gradient micro vortex and sound wave fluidization and application of the plant cell vesicle efficient extraction method. According to the plant cell vesicle extraction method provided by the invention, high-yield, high-purity and high-activity extraction of plant cell vesicles is realized through an active enzymolysis-acoustic cracking-three-dimensional gradient separation full-chain process, and the plant cell vesicle extraction method can be used for large-scale production. The method overcomes the defects of low yield and purity, chemical pollution, low extraction efficiency, uncontrollable vesicle size, easily damaged structure, long time and the like of the traditional extraction method, and can be used for producing and preparing more cosmetics and medicines containing plant cell vesicles on a large scale. The method systematically solves the global problem of extraction and application of the plant vesicles through multidisciplinary cross innovation (acoustics, fluid mechanics and molecular bionics), is high in extraction efficiency, good in activity effect and comprehensive and advanced in technical parameters, and has important significance on extraction and development of the plant cell vesicles.

The invention relates to the field of functional food and pharmaceutical preparations, and discloses an active multienzyme preparation with spleen and stomach strengthening effects. The solid beverage is prepared from the following components in parts by weight: 10 to 20 parts of radix astragali seu hedysari extract, 5 to 10 parts of rhizoma atractylodis macrocephalae extract, 10 to 20 parts of poria cocos extract, 5 to 10 parts of pericarpium citri reticulatae extract, 5 to 10 parts of fructus crataegi extract, 5 to 10 parts of malt extract, 5 to 10 parts of alpha-amylase, 5 to 10 parts of protease, 2 to 5 parts of cellulase, 2 to 5 parts of pectinase, 2 to 5 parts of lipase, 10 to 15 parts of prebiotics, 2 to 5 parts of xylitol and 3 to 8 parts of microcapsule wrapping materials. The compound preparation with high stability and multiple functions is prepared by scientifically combining traditional Chinese medicine extracts and multiple active enzymes, combining with reasonable addition of prebiotics and auxiliary materials, adopting a micro-capsule wrapping technology to protect enzyme activity and optimizing traditional Chinese medicine extraction, component mixing and forming processes.

The invention discloses a preparation method of a dry chemical reagent for creatine content determination. The preparation method comprises the following steps: S1, preparing a buffer reagent and a protective component; s2, dissolving the biological enzyme and the chromophoric substrate to form a dissolving reagent; s3, coating by using a biological film-forming material to form a coating reagent; s4, coating and printing the coating reagent in the pore channel of the specified bottom plate base material in a volume quantitative manner by virtue of equipment; the equipment comprises a single-channel type pipettor, a multi-channel type pipettor, a continuous multi-gear distributor or automatic dot printing equipment. S5, placing the specified bottom plate base material printed with the coating reagent in forced air drying equipment or drying equipment with the same efficiency; and S6, measuring the moisture content after drying and film forming, and judging whether substances containing biologically active enzymes and substrates in the reagent are completely locked to the position of the net-shaped cavernous body structure of the biological film forming material or not based on the moisture content. The invention further discloses application of the dry chemical reagent for determining the creatine content, and the dry chemical reagent is applied to a sample formed by urine, serum or tissue.

The invention discloses a nanofiber based on a polypeptide derivative and an enzymatic self-assembly method and application of the nanofiber based on the polypeptide derivative, the sequence of the polypeptide derivative is Nap-Gly-Phe-Phe-pTyr-Lys-Trp-Tyr-Gln-Asn-Met-Ile-Arg, and the Gly-Phe-Phe-pTyr is of an L configuration or a D configuration. The self-assembled polypeptide derivative disclosed by the invention contains an alkaline phosphatase restriction enzyme cutting site, and the self-assembled polypeptide derivative is self-assembled at a specific part with high enzyme specificity expression through enzyme catalysis to form nanofibers. The nanofiber hydrogel obtained through enzymatic treatment is carried out under physiological conditions, high temperature or ultraviolet irradiation is not needed, damage of high temperature to bioactive substances is avoided, the nanofiber hydrogel is more suitable for a temperature-sensitive in-vivo environment, toxic chemical cross-linking agents or photoinitiators do not need to be introduced, the cytotoxicity of the material is remarkably reduced, active enzymes or drugs can be embedded in situ, and the nanofiber hydrogel can be used for in-situ treatment. The biological function is maintained.

The invention provides a method for catalytically synthesizing phytosterol ester by using ionic liquid modified magnetic nano composite material immobilized lipase, which comprises the following steps: taking imidazolium ionic liquid modified magnetic beta-cyclodextrin organic-inorganic nano composite material immobilized lipase as a catalyst; esterification of phytosterol and fatty acid is catalyzed in a solvent-free system to synthesize the phytosterol ester. The carrier prepared by the method combines the characteristics of good biocompatibility of the beta-cyclodextrin and the ionic liquid, capability of stabilizing the active conformation of enzyme and the like, and also combines the characteristic of easy separation of a magnetic material. The immobilized enzyme has the characteristics of convenience in separation, high catalytic activity, good stability and the like, and can be used for catalyzing esterification reaction of phytosterol and fatty acid to prepare phytosterol ester. The invention provides a novel green and efficient phytosterol ester synthesis method.

The invention discloses a preparation method of a dry chemical reagent for determining the content of beta-hydroxybutyric acid in urine. The preparation method comprises the following steps: S1, preparing a buffer reagent and a protective component; s2, dissolving the biological enzyme and the chromophoric substrate to form a dissolving reagent; s3, coating by using a biological film-forming material to form a coating reagent; s4, by means of equipment, the coating reagent is coated and printed in a pore channel of a specified bottom plate base material in a volume quantification mode; the equipment comprises a single-channel type pipettor, a multi-channel type pipettor, a continuous multi-gear distributor or automatic dot printing equipment. S5, placing the specified bottom plate base material printed with the coating reagent in forced air drying equipment or drying equipment with the same efficiency; and S6, measuring the moisture content after drying and film forming, and judging whether substances containing biologically active enzymes and substrates in the reagent are completely locked to the position of the net-shaped cavernous body structure of the biological film forming material or not based on the moisture content. The invention further discloses application of the dry chemical reagent for determining the content of beta-hydroxybutyric acid in urine, and the dry chemical reagent is applied to a sample formed by urine.

The invention belongs to the technical field of molecular biology, and particularly relates to a gene for encoding glucose dehydrogenase, a biological material and application of the gene and the biological material. The invention provides a gene for encoding glucose dehydrogenase. The gene comprises a nucleotide sequence as shown in SEQ ID NO: 1. By expressing the gene, the obtained glucose dehydrogenase can better catalyze NAD < + > or NADP < + > to generate NADPH (Nicotinamide Adenine Dinucleotide Phosphate). According to the invention, escherichia coli Rosetta (DE3) is utilized to express the gene for coding the glucose dehydrogenase, and the activity of the generated active enzyme is 3285.714 U / mg, which is obviously higher than the catalytic activity of the glucose dehydrogenase generated by escherichia coli BL21 (DE3).

The invention belongs to the technical field of bioengineering and microbiomics, and particularly relates to an acquisition method and application of a specific metabolic substrate of lactobacillus crispatus and application of a specific carbohydrate active enzyme CAZzyme gene and a polysaccharide utilization site (PUL). Genomes of lactobacillus crispatus and lactobacillus inertus are obtained from a public database, gene annotation is carried out on the genomes of the lactobacillus crispatus and the lactobacillus inertus, and a random forest model and a KEGG metabolic pathway diagram are combined to find specific CAZzyme genes, PUL and other genes with metabolic functions of the lactobacillus crispatus; a substrate which only lactobacillus crispatus can metabolize is searched based on the genome difference of the two bacteria, and the ability of the obtained substrate for promoting the growth of lactobacillus crispatus and inhibiting the growth of inert lactobacillus is evaluated through experiments. Growth and reproduction of the lactobacillus crispatus are promoted through the substrates, so that the lactobacillus crispatus accounts for a higher proportion in vagina microecology, the vagina is protected, and reproduction of other unfavorable bacteria is reduced. The assisted reproduction success rate is improved.

The invention relates to a detergent composition which can be produced using a first and a second enzyme preparation, wherein the first enzyme preparation comprises a cellulase, and the second enzyme preparation comprises an amylase, characterized in that the first and second enzyme preparations at least 0.5% by weight of the respective enzyme, based on the total mass of active enzyme, at least 45% by weight water and 5% to 45% polyol by weight, based on the total mass of the enzyme preparation. The detergent composition is characterized by improved storage stability.

The present invention relates to the field of fertilizers, and specifically discloses a small molecule peptide organic composite synergistic foliar fertilizer and a preparation method thereof. The foliar fertilizer comprises the following raw materials, calculated by mass percentage of 100%, including 2-6% small molecule peptides, 15-25% fulvic acid, 4-7% microbial natural active enzymes, 3-8% adhesives, 1-3% stabilizers, and the remainder is deionized water; the adhesive is prepared by oxidation and phosphorylation of non-monosaccharides. The composite synergistic foliar fertilizer of the present invention is mainly composed of small molecule peptides, fulvic acid and microbial natural active enzymes, and improves the growth quality of plants through the interaction between the three, and improves the utilization rate of foliar fertilizer on the leaf surface through the adhesive.

The invention relates to a preparation method and application of a high-activity SOD enzyme compound raw material. The preparation method of the high-activity SOD enzyme compound raw material comprises the following steps: (1) adding SOD enzyme into a zinc glycinate solution, and carrying out ultrasonic treatment; (2) collecting the reaction solution, and performing dialysis and / or ultrafiltration to obtain filtrate; (3) adjusting the pH value of the filtrate, adding a hydrolyzed protein zinc solution into the filtrate, and fully stirring to obtain a mixed solution; (4) adding a sodium carbonate and / or sodium bicarbonate buffer solution into the mixed solution, and adjusting the pH value; and (5) performing ultrafiltration concentration and freeze-drying to obtain the SOD enzyme compound raw material. According to the preparation method, high activity of SOD can be kept in the preparation and storage processes of the cosmetics, so that the anti-aging effect and the whitening effect of the SOD applied to the cosmetics are enhanced.

The invention belongs to the technical field of enzyme baths, and particularly relates to a compound conditioning type enzyme bath composition and a fermentation method thereof.The compound conditioning type enzyme bath composition is prepared from cedarwood powder, folium artemisiae argyi powder, safflower powder, dried ginger powder, radix angelicae sinensis powder, radix salviae miltiorrhizae powder, herba leonuri powder, rhizoma chuanxiong powder, radix saposhnikoviae powder, garden balsam stem powder, natural active enzymes, mineral substances, nutrient substances and water; the health care product is prepared from the cedarwood powder, the folium artemisiae argyi powder, the safflower powder, the dried ginger powder, the radix angelicae sinensis powder, the radix salviae miltiorrhizae powder, the herba leonuri powder, the rhizoma chuanxiong powder, the radix saposhnikoviae powder, the phryma leptostachya powder, the natural active enzyme, the mineral substances and the nutrient substances, can comprehensively condition the body, promote blood circulation and relieve fatigue, and has positive influence on body health.

The invention discloses nutrient liquid for supplementing energy to human cells and a preparation method of the nutrient liquid, and belongs to the field of functional foods and beverages. The preparation method comprises the following steps: equivalently mixing fructus hippophae, longan pulp, Chinese dates, Chinese wolfberry fruits, mulberries, malt, raspberries and honey, sealing and fermenting, and filtering to obtain an active enzyme a stock solution; mixing Chinese yam, hawthorn, wheat, cinnamon, mulberry leaf, emblic leafflower fruit, kelp, momordica grosvenori, honeysuckle, kudzuvine root, rhizoma polygonati, black sesame, houttuynia cordata, purslane, dandelion, lucid ganoderma, ginseng, American ginseng, dogwood, eucommia ulmoides leaf and cistanche according to an equal amount to obtain a fermentation base material, mixing the fermentation base material with the active enzyme a stock solution, performing anaerobic fermentation, and performing low-temperature drying to obtain enzyme b; the stock solutions of the enzyme b and the active enzyme a are mixed, cooked and filtered to obtain mixed juice, a unique formula raw material is developed, and the nutrient solution with the good dissolved oxygen content is obtained through the sealed fermentation technology and the anaerobic fermentation technology.

The invention relates to the technical field of nutrient synergistic chelation and slow release, in particular to a preparation process of an organic water-soluble fertilizer containing polymeric amino acid, which comprises the following steps: preparing the polymeric amino acid, modified potassium fulvate, nanoscale microelement chelate and other raw materials in percentage by weight, and further comprises a bioactive enzyme compound; the preparation method comprises the following steps: modifying potassium fulvate, and adding nano titanium dioxide and carbon nanotubes; preparing a chelate, and adding a novel surfactant; when the compound is synthesized, biological active enzyme is added; finally, mixing and preparing, sequentially adding the materials, stirring, adding the preservative, and filtering to obtain the water-soluble fertilizer, and each step has clear temperature, time and operation requirements. The water-soluble fertilizer is scientific in formula, polymeric amino acid and the like have a synergistic effect, and the utilization rate of the fertilizer is increased; the water-retaining agent is good in water retention, and the biological active enzyme improves the soil; the preparation process is stable and environment-friendly, can improve crop yield and quality, reduces pollution, and brings economic and ecological double benefits.

This application discloses a method, a biosensor, and a related device for detecting the concentration of a target analyte. The biosensor includes a first working electrode and a second working electrode. When the tip of the biosensor is implanted subcutaneously with a hard needle, the second working electrode is closer to the needle tip than the first working electrode. An active enzyme is disposed on the first working electrode. After implantation, the first working electrode generates a first current, which includes the current generated by the reaction between the target analyte and the active enzyme, and the current generated by the reaction between an interfering substance and the first working electrode. An inactivating enzyme is disposed on the second working electrode. After implantation, the second working electrode generates a second current, which includes the current generated by the reaction between the interfering substance and the second working electrode. Thus, by using the first and second currents, the influence of interfering substances can be eliminated, improving the accuracy of the target analyte concentration calculation.

This invention discloses a highly efficient extraction method for plant cell vesicles based on the synergistic effect of three-dimensional gradient microvortex and acoustic fluidization, and its application. The plant cell vesicle extraction method provided by this invention achieves high yield, high purity, and high activity of plant cell vesicles through a full-chain process of active enzymatic hydrolysis, acoustic lysis, and three-dimensional gradient separation, enabling large-scale production. This method overcomes the shortcomings of traditional extraction methods, such as low yield and purity, chemical contamination, low extraction efficiency, uncontrollable vesicle size and easily damaged structure, and long extraction time. It allows for the large-scale production of more cosmetics and pharmaceuticals containing plant cell vesicles. This invention systematically solves the global challenge of plant vesicle extraction and application through multidisciplinary innovation (acoustics, fluid mechanics, and molecular biomimetic), achieving high extraction efficiency, good activity, and leading technical parameters, which is of great significance for the extraction and development of plant cell vesicles.

The invention belongs to the technical field of beverage processing, and discloses a natural electrolyte functional vinegar salt honey beverage and a non-thermal sterilization preparation process thereof. The natural electrolyte functional vinegar salt honey beverage comprises the following components in percentage by mass: 25-35% of millet vinegar, 12-18% of pagoda flower honey, 0.02-0.05% of table salt, 0.3-1% of auxiliary materials and the balance of water, the auxiliary materials are selected from at least two of mulberry fruits, fried almonds and cane sugar. According to the invention, a'vinegar soaking-salt explosion 'synergistic process is adopted, so that non-thermal sterilization is realized, thermosensitive components are prevented from being damaged, and the problems that existing vinegar drinks are too strong in irritation and single in flavor and active enzymes are easily damaged by high-temperature processing of honey can be effectively solved; the prepared natural electrolyte functional vinegar salt honey beverage can be drunk as supplement of electrolyte after exercise, does not cause metabolic burden, and is suitable for long-term drinking.

The present invention discloses a method for enzymatic hydrolysis extraction of a wild ginseng-like product, belonging to the technical field of wild ginseng. Some rare saponins in wild ginseng can promote cell growth and division and improve the body. Through specific active enzymes, the prototype saponins can be enzymatically hydrolyzed to generate different rare saponins. In the present invention, through the action of radio frequency plasma, a mixed gas of α-linolenic acid vapor and nitrogen is used to treat the ultrafiltration membrane, thereby introducing double bond groups onto the ultrafiltration membrane; an esterification reaction occurs between p-hydroxycinnamic acid and cellulase to generate a product with reactive activity; these products undergo graft polymerization with the double bonds on the surface of the ultrafiltration membrane to form a more stable and more functional modified ultrafiltration membrane; the present invention can effectively separate and extract the target substances through a combination of enzymatic hydrolysis and ultrafiltration membrane fractionation; the molecular weight distribution of the peptides released during the enzymatic hydrolysis process is within a certain range, which helps to precisely separate these substances through the ultrafiltration membrane, and the extraction rate is high.

A multi-nozzle food 3D printing device with a controllable freezing and curing function comprises a cooling control mechanism used for curing food and a rotating mechanism installed in a 3D printer nozzle, the cooling control mechanism comprises a condenser, the condenser surrounds the periphery of a charging barrel of a 3D printer, and a refrigerant is liquefied through the condenser to release heat, so that the temperature of the nozzle is increased; the rotating mechanism comprises an outer shell and a driving mechanism, the driving mechanism drives the outer shell to rotate, a plurality of conveying pipes are arranged in the outer shell, one ends of the conveying pipes are connected, openings of the conveying pipes are rotationally connected with an opening in the bottom of the charging barrel, and the other ends of the conveying pipes are communicated with the nozzle. According to the invention, the cooling temperature of the freezing chamber is controlled by changing the pressure intensity of the refrigerant, and the problem of shape fixation of food materials such as protein and active enzyme is solved; the rotating mechanism is arranged in the spray head, the motor drives the material to flow out through the spray nozzle in a rotating mode, and the printed material is rapidly formed in cooperation with the cooling control mechanism.

The present invention relates to a biocatalyst for organic synthesis, comprising a controlled porosity silica (CPS) as a support material, wherein the pore diameter is from about 20 to about 100 nm, the support material comprises an amino-functionalized surface, and one or more catalytically active enzymes immobilized on the support material. The present invention also relates to the use of such biocatalysts and a method for their production.

The present invention relates to a biocatalyst for organic synthesis comprising controlled porosity silica (CPS) as a support material wherein the pore diameter is between about 20 and about 100 nm; the support material comprises an amino-functionalized surface; and one or more catalytically active enzymes immobilized on the support material. The invention also relates to the use of such a biocatalyst and to a method for the production thereof.