16 results about "Active enzyme" patented technology

This application discloses a method, a biosensor, and a related device for detecting the concentration of a target analyte. The biosensor includes a first working electrode and a second working electrode. When the tip of the biosensor is implanted subcutaneously with a hard needle, the second working electrode is closer to the needle tip than the first working electrode. An active enzyme is disposed on the first working electrode. After implantation, the first working electrode generates a first current, which includes the current generated by the reaction between the target analyte and the active enzyme, and the current generated by the reaction between an interfering substance and the first working electrode. An inactivating enzyme is disposed on the second working electrode. After implantation, the second working electrode generates a second current, which includes the current generated by the reaction between the interfering substance and the second working electrode. Thus, by using the first and second currents, the influence of interfering substances can be eliminated, improving the accuracy of the target analyte concentration calculation.

The application relates to the technical field of metal recovery, in particular to a metal removal and recovery method for waste titanium anode plates; nanometer particles are subjected to a coating operation, cations are introduced to graft the coated nanometer particles, grafted nanometer particles are obtained, and the grafted nanometer particles are pretreated to obtain initial nanometer particles. In the application, the metal recovery of the waste titanium anode plates is realized through the synergistic effect of magnetic nanometer particle synthesis, functional modification and biological enzyme directional degradation; the surface of the nanometer particles is subjected to double-layer coating of silicon dioxide-polyacrylic acid and synergistic modification of carboxyl and amino groups, the adsorption capacity and selectivity to noble metal ions are enhanced, the noble metal ions are reduced to metal states through catalysis of an active enzyme solution, an integrated process of adsorption to degradation and then magnetic separation is formed between the noble metal ions and the functional nanometer particles, and after the titanium matrix is regenerated through pickling, high-value recovery and utilization of metal materials can be realized through smelting or powder metallurgy.

The application discloses a plant selenoprotein enzyme peptide composition, effervescent tablets and application thereof, and relates to the field of functional food, and comprises the following components in parts by weight: selenium-rich shepherd's purse powder 5-12 parts; comprehensive fruit and vegetable fermentation powder 8-15 parts; soybean peptide powder 3-10 parts; comprehensive digestive enzyme 05-3 parts; and pharmaceutically or dietetically acceptable excipients; the plant selenoprotein enzyme peptide composition, the effervescent tablets and the application thereof are scientifically combined with organic selenium (selenium-rich shepherd's purse powder), active enzymes and small-molecule metabolites generated by fermentation of various fruits and vegetables, soybean peptides easy to absorb and prebiotics. Selenium is the core of the antioxidant system, peptides and fermentation products provide nutritional substrates and regulatory signals, prebiotics improve the intestinal microenvironment, and multiple components synergistically act on multiple pathways such as immunity and metabolism.

This invention discloses an intelligent fermentation device and method for processing plant active enzyme beverages, relating to the field of fermentation device technology. It includes a tank and a rotating stirring mechanism inside it. One end of the tank is equipped with a drive mechanism to rotate the stirring mechanism. The stirring mechanism inside the tank periodically stirs the fermenting material to promote fermentation. The device is designed as a drum, and the drive mechanism can drive the stirring mechanism to rotate independently or simultaneously drive the tank and stirring mechanism to rotate in opposite directions, completely removing sediment adhering to the bottom of the tank's inner wall, thus allowing for more complete fermentation. Furthermore, to prevent gas buildup inside the tank after fermentation, which could increase internal pressure and cause leakage of the fermentation liquid from the stirring mechanism's shaft, the shaft sleeve is pressurized. This utilizes greater external air pressure to reverse and confine the fermentation liquid inside the tank, effectively preventing contamination.

This invention provides a hydrophilic bifunctional probe and its synthesis and application. Specifically, this invention provides a hydrophilic bifunctional probe (6-(1-aminoethyl)naphthyl-2-yl)glycine (N1) and its corresponding fluorescent molecule (6-acetylnaphthyl-2-yl)glycine (N2). This pair of molecules in this invention exhibits high photostability and water solubility, and can be used in aqueous luminescent materials, biofluorescent probes, and biofluorescent imaging. It can be applied to fluorescence-based high-throughput screening systems to identify strains of highly active enzymes through quantitative detection in trace systems. The structures of the hydrophilic bifunctional probe and its corresponding fluorescent molecule are as follows: [structures omitted].

This application relates to the field of agricultural fertilizer technology and discloses a preparation process for a high-efficiency, low-temperature growth-promoting tetranitrogen liquid fertilizer, comprising the following steps: Step 1, selecting high-quality sheepskin as the organic nitrogen source and treating the sheepskin; Step 2, hydrolyzing the sheepskin at 55℃ using microorganisms and active enzymes for 24 hours; Step 3, performing preliminary filtration on the hydrolyzed mixture; Step 4, further purifying the amino acid liquid through multi-stage filtration; Step 5, concentrating the amino acid liquid to 40% concentration using vacuum evaporation technology; Step 6, mixing the concentrated amino acid liquid with urea ammonium nitrate solution, magnesium sulfate, ferrous sulfate, seaweed concentrate, nitrification inhibitor, and excipients in a specific ratio. By scientifically proportioning the high-concentration amino acid liquid with urea ammonium nitrate, magnesium sulfate, ferrous sulfate, seaweed concentrate, and nitrification inhibitor, comprehensive nutritional support is provided for crops, effectively improving crop growth rate, stress resistance, and yield.

This invention relates to the field of medicinal plant cultivation technology, specifically a cultivation method and application for significantly increasing the mannan content of Dendrobium officinale. The method includes: selecting a suitable cedar forest environment, fixing Dendrobium officinale seedlings using a soilless planting method, watering regularly, and spraying with a 200-fold diluted indigenous probiotic cultivation enzyme every 10 days, for a total of 10 sprays. The enzyme is fermented from native microorganisms in natural forest soil and plant materials, containing Bacillus amyloliquefaciens, yeast, active enzymes, and various minerals. This invention can significantly increase the yield of Dendrobium officinale and the content of active ingredients such as mannan and total amino acids, achieving green, efficient, and sustainable cultivation.

This invention provides an energy-saving and environmentally friendly negative oxygen ion air purification device, comprising a frame, a bioactive enzyme extraction composite material layer fixedly connected to the front surface of the frame, a silk cloth detachably connected to the front surface of the bioactive enzyme extraction composite material layer, ventilation holes evenly distributed at the bottom of the frame, and connecting plates symmetrically fixedly connected to the inner sidewalls of the frame, with hanging holes on the rear surface of the connecting plates. The bioactive enzymes in the bioactive enzyme extraction composite material layer of this invention target water and oxygen molecules in indoor air, continuously providing active electrons to oxygen molecules through biomimetic catalysis, promoting their conversion into negative oxygen ions. These negative oxygen ions can effectively decompose organic pollutants and promote the settling of suspended particulate matter in the air. The entire purification process requires no electricity, achieving zero power consumption and noiseless operation.

This invention relates to a highly active Cas enzyme, its preparation method, and its applications. Specifically, this invention provides a fusion protein containing a CL7 tag, from which high-purity LbCas12a nuclease can be obtained through only two chromatographic steps, including CL7 / Im7 chromatography. The LbCas12a nuclease obtained through the above steps exhibits high activity, stability, and thermostability.

This invention discloses a cephalothinyl synthase mutant and its application in the biosynthesis of cephalothinyl synthase, belonging to the fields of enzyme engineering and synthetic biology. Through rational design and mutation screening of tobacco-derived cephalothinyl synthase (NtCBTS), this invention obtained a mutant with significantly improved catalytic performance. Specifically, the cephalothinyl synthase yield of strain containing the mutant NtCBTS_K208R-A268C-K353R was increased by 231.18% compared to strains containing NtCBTS. Applying this mutant to a *Saccharomyces cerevisiae* expression system achieved highly efficient biosynthesis of cephalothinyl synthase, significantly improving the production level of the target product. The highly active enzyme mutant and its application strategy provided by this invention effectively improve the biosynthetic efficiency of cephalothinyl synthase, providing important technical support for constructing high-yield yeast cell factories and promoting its industrial production.

Disclosed in the present application are a method for measuring the concentration of a target analyte, a biosensor, and a related apparatus. The biosensor disclosed in the present application comprises a first working electrode and a second working electrode. When a tip portion of the biosensor is implanted subcutaneously along with a hard needle, the second working electrode is closer to a tip of the hard needle than the first working electrode. The first working electrode is provided with an active enzyme. The first working electrode generates a first current after being implanted subcutaneously, and the first current comprises: a current generated by the reaction between the target analyte and the active enzyme and a current generated by the reaction between an interferent and the first working electrode. The second working electrode is provided with an inactive enzyme. The second working electrode generates a second current after being implanted subcutaneously, and the second current comprises: a current generated by the reaction between the interferent and the second working electrode. In this way, the influence of the interferent can be eliminated by means of the first current and the second current, thereby improving the calculation accuracy of the concentration of the target analyte.

The present application relates to the field of freeze-dried preparations of leeches, and provides a freeze-dried powder containing multiple active enzymes and a preparation method thereof, to solve the problem that the prior art cannot simultaneously ensure the release of active ingredients and the maintenance of the stability of active ingredients, comprising the following steps: S100, cooling leeches to obtain freeze-dried leeches; S200, putting the freeze-dried leeches into a solution system, crushing, centrifuging, and taking the supernatant; the pH value of the solution system is 6.5-8.5; the solution system at least comprises water; the crushing speed is 150-250 r / min, and the time is 5-8 min; S300, after the supernatant is separated and purified, freeze-drying is performed to obtain the freeze-dried powder of leeches. The present application adopts a freeze-crushing process, and after freezing, not only can the crushing operation be simply realized, but also the loss of active ingredients caused by high temperature can be reduced by utilizing the crushing process at low temperature of raw materials.

This invention belongs to the field of aquaculture technology and discloses a method for preparing a compound metabiotic to improve the stress resistance of aquatic animals and its application in feed. The invention discloses a method for preparing a compound metabiotic that employs a spatiotemporal sequential fermentation strategy of "first propagation, then induction, and finally compensation": First, Bacillus subtilis aerobic fermentation degrades the substrate to produce specific signal peptides; then, Lactobacillus plantarum and Saccharomyces cerevisiae are introduced, utilizing the previous metabolites to induce Lactobacillus plantarum to efficiently synthesize antimicrobial peptides and organic acids, and combined with the metabolic compensation effect of Saccharomyces cerevisiae, significantly enhancing the functional component gradient of the metabiotic. The compound metabiotic prepared by this invention has high concentrations of organic acids, active enzyme systems, and high release rates of β-glucan, which can significantly improve the stress resistance and survival rate of aquatic animals such as shrimp under extreme environments such as low salinity, high ammonia nitrogen, and low oxygen, solving the industry pain point of probiotic activity loss during high-temperature feed pelleting.

This invention discloses a highly active cellulase preparation for the enzymatic hydrolysis of straw, belonging to the field of straw enzymatic hydrolysis technology. The highly active cellulase preparation comprises: an enzyme and an additive; the enzyme includes cellulase, xylanase, and pectinase; the additive is obtained by adding monomers via free radical polymerization using modified cyclodextrin as the initiating molecule; the monomers are quinoline monomers and hydrophilic monomers; the highly active cellulase preparation has a high efficiency in enzymatic hydrolysis and can efficiently and easily recover highly active enzyme preparations.

The application belongs to the field of pharmaceutical chemistry, and particularly relates to a method for biosynthesizing lanatoside C based on acetyltransferase to deacetylcardiopine, the application obtains soluble target protein with a purity of >90%, at least one candidate enzyme can catalyze the generation of lanatoside C, and the product can be confirmed by HPLC and LC-MS. The optimal reaction condition of each enzyme is obtained, and the target conversion rate is >50%. The Km and Vmax values of the best active enzyme are obtained, and the affinity and catalytic efficiency of the enzyme and the substrate are evaluated.

The application discloses a low-temperature fast-acting enzyme synergistic type concentrated laundry liquid and a preparation method thereof. The laundry liquid comprises an enzyme-carrier complex formed by reversible combination of a temperature-sensitive anchoring enzyme carrier and a low-temperature active enzyme; the temperature-sensitive anchoring enzyme carrier is a block copolymer with a three-segment structure of a hydrophobic block, a hydrophilic block and an enzyme anchoring block, wherein the hydrophobic block is polymerized from a methyl methacrylate monomer with 12-15 carbon atoms, the hydrophilic block is polymerized from a polyethylene glycol (methyl) acrylate monomer, and the enzyme anchoring block is polymerized from a vinyl monomer containing a phenylboronic acid group. The hydrophobic block is inserted into a sebum dirt crystal lattice through phase transition trapping at low temperature, and the enzyme anchoring block precisely anchors the enzyme to the dirt interface, so that the physical disturbance and enzymatic cutting are synergistically removed.