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6 results about "Carbohydrate-binding module" patented technology
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In molecular biology, a carbohydrate-binding module (CBM) is a protein domain found in carbohydrate-active enzymes (for example glycoside hydrolases). The majority of these domains have carbohydrate-binding activity. Some of these domains are found on cellulosomal scaffoldin proteins. CBMs were previously known as cellulose-binding domains. CBMs are classified into numerous families, based on amino acid sequence similarity. There are currently (June 2011) 64 families of CBM in the CAZy database.
A fusion cholesterol oxidase and its use in a biosensor element
PendingCN122103365AMicrobiological testing/measurementMaterial analysis by electric/magnetic meansCarbohydrate-binding proteinOxidative enzyme
The present application constructs a fusion protein by utilizing the specific affinity adsorption property of a carbohydrate binding module (CBM) to cellulose, the fusion protein comprising a cholesteric oxidase and a carbohydrate binding module (CBM), and then uses nanocellulose membranes as the immobilization carrier of the fusion protein for preparing a biosensor element, and the obtained biosensor element can exhibit good catalytic properties and detection stability.
Owner:BIOLOGY INST OF SHANDONG ACAD OF SCI
Alpha-amylases, baking compositions and methods
The present invention relates to isolated polypeptides having alpha-amylase activity, polypeptides comprising an alpha-amylase catalytic domain and a carbohydrate binding module, and polynucleotides encoding the polypeptides, compositions comprising said polypeptides, as well as polynucleotides and nucleic acid constructs or vectors encoding the polypeptides, and host cells comprising the polynucleotides or constructs / vectors, as well as methods of producing and using the polypeptides.
Owner:NOVOZYMES AS
A fusion uric acid oxidase and its use in a biosensing element
ActiveCN119613566BAntibody mimetics/scaffoldsMicroorganism based processesCarbohydrate-binding proteinOxidative enzyme
The application provides a fusion uric acid oxidase and application thereof in a biosensing element. The fusion uric acid oxidase comprises a carbohydrate binding module and a uric acid oxidase, and the fusion uric acid oxidase can be made into a biosensing element for uric acid detection, and has the advantages of high sensitivity and good stability.
Owner:BIOLOGY INST OF SHANDONG ACAD OF SCI
Thermostable binding scaffolds
The present invention features thermostable protein binding scaffolds containing framework regions and variable loop regions that can be mutagenized to bind a desired target. The scaffolds are derived from the Carbohydrate Binding Module Family 32 (CBM32) protein domain of Clostridium perfringens hyaluronidase (NagH). The robust framework of the scaffolds described herein allows for the development of custom, high performance affinity chromatography resins compatible with the harsh conditions of process-scale applications that can be adaptable to a wide diversity of target substrates.
Owner:NECTAGEN
Carbohydrate binding module and application thereof
The invention belongs to the technical field of biology, and particularly relates to a carbohydrate binding module and application thereof. The invention discloses a carbohydrate binding module for the first time, and the carbohydrate binding module can be bound with insoluble polysaccharide and can be used for preparing and / or preparing tag protein, immobilizing target protein and / or preparing an immobilized compound.
Owner:BEIJING DABEINONG TECHNOLOGY GROUP CO LTD +1
Carbohydrate binding module variants and hybrid polypeptides comprising same
The present invention relates to cellobiohydrolase variants and carbohydrate binding module variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Owner:NOVOZYMES AS