12 results about "Carbohydrate-binding protein" patented technology

The invention discloses an endoglucanase truncated mutant and an application of the endoglucanase truncated mutant. According to the invention, based on endoglucanase BsEGL from bacillus subtilis DLG, a truncated mutant is constructed by deleting a carbohydrate binding domain (CBM) of the endoglucanase BsEGL, and the truncated mutant is named as BsEGL [delta] CBM. Compared with BsEGL, the truncated mutant BsEGL delta CBM has the advantages that the thermal stability, the pH stability, the organic solvent tolerance and the like are remarkably improved, and the activity of degrading phosphoric acid expanded cellulose and corncob xylan is obtained. In addition, the enzymatic activity of the truncated mutant BsEGL delta CBM is remarkably improved by 6.41%, and the catalytic efficiency is remarkably improved. Due to the characteristics, the mutant can be suitable for industrial processes such as feed processing, papermaking, biological energy sources, spinning and food brewing, and is particularly suitable for enzyme catalytic reaction under extreme environments such as high temperature and low pH. The invention provides an enzyme preparation tool with excellent performance for efficient degradation of cellulose biomass, and has important application value.

The present application constructs a fusion protein by utilizing the specific affinity adsorption property of a carbohydrate binding module (CBM) to cellulose, the fusion protein comprising a cholesteric oxidase and a carbohydrate binding module (CBM), and then uses nanocellulose membranes as the immobilization carrier of the fusion protein for preparing a biosensor element, and the obtained biosensor element can exhibit good catalytic properties and detection stability.

The present invention relates to isolated polypeptides having alpha-amylase activity, polypeptides comprising an alpha-amylase catalytic domain and a carbohydrate binding module, and polynucleotides encoding the polypeptides, compositions comprising said polypeptides, as well as polynucleotides and nucleic acid constructs or vectors encoding the polypeptides, and host cells comprising the polynucleotides or constructs / vectors, as well as methods of producing and using the polypeptides.

The present invention provides a modified protein sequence, namely a recombinantly expressed glycan-binding protein. This glycan-binding protein is a variant of the Sclerotium Rolfsii lectin and has been modified to include features such as increased molecular stability, a reduction in N-terminal methionine impurities, and improved protein conjugation with other biological and chemical agents.

The invention provides a novel streptococcus group A oligosaccharide antigen and glycobinding protein vaccine, which is characterized in that oligosaccharide antigen containing 30-70% of N-acetyl glucamine multi-rhamnose core skeleton structure is accurately prepared through chemical synthesis, and is efficiently coupled with KLH (the loading capacity is about 215 oligosaccharide / KLH molecules); safe, efficient and broad-spectrum immune protection is realized; safety: the GlcNAc side chain modification design avoids autoimmune risks (such as acute rheumatic fever heat); high efficiency: the animal experiment antibody titer reaches 1: 12, 800; the broad spectrum is as follows: the highly conservative universal GAS oligosaccharide antigen can universally cover various serotypes, and the key synthetic route (Scheme 2, 3, 16 and 20, the yield is 22%-96%) and the loading capacity verification (HPAEC) support the technical feasibility.

The present invention aims to provide a fucose-binding protein that shows improved productivity in cases of expression in a host such as Escherichia coli, improved binding affinity to a fucose-containing sugar chain such as a sugar chain containing a structure composed of Fucα1-2Galβ1-3GlcNAc and / or Fucα1-2Galβ1-3GalNAc, and / or improved thermal stability. The above object is achieved by deleting a plurality of amino acid residues in the C-terminal side of the amino acid sequence of the fucose-binding protein BC2LCN of SEQ ID NO: 1, and, when necessary, substituting the glycine residue at position 36 in SEQ ID NO: 1 with a cysteine residue, substituting the glutamine residue at position 39 in SEQ ID NO: 1 with a leucine residue or methionine residue, substituting the glutamine residue at position 65 in SEQ ID NO: 1 with a leucine residue, substituting the cysteine residue at position 72 in SEQ ID NO: 1 with a glycine residue or alanine residue, substituting the glutamic acid residue at position 81 in SEQ ID NO: 1 with a cysteine residue, glutamine residue, histidine residue, or methionine residue, and / or substituting the glycine residue identified as the residue at position 36 in SEQ ID NO: 1 with a cysteine residue.

The application provides a fusion uric acid oxidase and application thereof in a biosensing element. The fusion uric acid oxidase comprises a carbohydrate binding module and a uric acid oxidase, and the fusion uric acid oxidase can be made into a biosensing element for uric acid detection, and has the advantages of high sensitivity and good stability.

The present invention features thermostable protein binding scaffolds containing framework regions and variable loop regions that can be mutagenized to bind a desired target. The scaffolds are derived from the Carbohydrate Binding Module Family 32 (CBM32) protein domain of Clostridium perfringens hyaluronidase (NagH). The robust framework of the scaffolds described herein allows for the development of custom, high performance affinity chromatography resins compatible with the harsh conditions of process-scale applications that can be adaptable to a wide diversity of target substrates.

The invention discloses a method for identifying glycosyltransferase or glycosidase specific enzyme participating in DNA / RNA glycosylation, which is characterized in that oxidation labeling is carried out on glycosylation modification of DNA / RNA molecules, and solid phase coupling and enzyme digestion strategies are combined, so that enzyme or sugar binding protein specifically bound with glycosylation DNA / RNA can be efficiently and accurately captured, and the specific enzyme of glycosyltransferase or glycosidase can be identified. The interference caused by non-specific binding is obviously reduced. The method is not only suitable for various biological samples including tissue slices, blood and cell samples, but also compatible with subsequent mass spectrometric analysis, and high-throughput identification and function annotation of targeted proteins are realized. The technology provides a brand new tool for analyzing the action mechanism of DNA / RNA sugar modification in epigenetic regulation and control, and has important value for promoting the research of related disease mechanisms and the development of targeted therapy strategies.

The invention belongs to the technical field of biology, and particularly relates to a carbohydrate binding module and application thereof. The invention discloses a carbohydrate binding module for the first time, and the carbohydrate binding module can be bound with insoluble polysaccharide and can be used for preparing and / or preparing tag protein, immobilizing target protein and / or preparing an immobilized compound.

The application provides a kind of nanobody specifically binding maltose binding protein and application, belong to the field of biotechnology.Nanobody can recognize the new epitope on MBP different from maltose binding pocket, so its binding with MBP is not affected by the presence or absence of maltose.This makes the purification process can be carried out in the buffer containing maltose, and elution without adding maltose, significantly simplify the process and avoid foreign sugar pollution, realize the efficient purification without sugar elution, stable protein complex formation and target protein release under mild conditions, significantly improve the applicability and reliability of MBP fusion protein in purification, structure analysis and function research.

The present invention relates to cellobiohydrolase variants and carbohydrate binding module variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.